Gel Doc XR and ChemiDoc XRS Systems Hardware Instruction Manual Rev G

Regulatory Notice IMPORTANT: The Gel Doc XR, and ChemiDoc XRS are designed and certified to meet ENC1010, the internationally accepted electrical safety standards and EMC regulations and TUV. Certified products are safe to use when operated in accordance with the instruction manual. This instrument should not be modified or altered in any way. Modification or alteration of this instrument will: 1. 2. 3.

Void the manufacturer’s warranty. Void the regulatory certifications. Create a potential safety hazard.

NOTE: Bio-Rad Laboratories is not responsible for any injury or damage caused by use of this instrument for purposes other than those for which it is intended, or by modifications of the instrument not performed by Bio-Rad Laboratories or an authorized agent.

Richtlinien WICHTIG: Dieses Geraet, von Bio-Rad Laboratories konstruiert, besitzt das Zertifikat ENC1010 anerkannt von dem internationalen elektrischen Sicherheitsstandard EMC und unterliegt dessen Richtlinien und TUV. Die Geraete sind sicher im Gebrauch, wenn die vorliegende Bedienungsanleitung beachtet wird. Dieses Geraet darf auf keinen Fall veraendert werden. Eventuelle Veraenderungen fuehren zu: 1. 2. 3.

Ungueltigkeit der Herstellergarantie Ungueltigkeit des Zertifikats Eventuelles Sicherheitsrisiko

Bemerkung: Bio-Rad Laboratories haftet nicht fuer Verletzungen an Personen oder Sachschaeden, hervorgerufen durch eine nicht vorhergesehene Benutzung des Geraetes. Ebenso uebernimmt BioRad Laboratories keine Haftung fuer Veraenderungen, die von nicht authorisierten Personen durchgefuehrt wurden.

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TABLE OF CONTENTS HARDWARE INSTRUCTION MANUAL ...1 SECTION 1 INTRODUCTION...7 1.1 COMPUTER REQUIREMENTS ...7 1.2 PC ...7 1.3 MACINTOSH ...7 SECTION 2 IMPORTANT SAFETY INFORMATION...8 2.1 IMPORTANT NOTICE ...8 2.2 POWER SAFETY INFORMATION ...8 SECTION 3 PRODUCT DESCRIPTION ...9 3.1 CCD CAMERA AND LENSES ...9 3.2 DARKROOM ENCLOSURE, EMISSION FILTERS, AND ILLUMINATION SOURCES ...9 3.3 PCI INTERFACE CARDS ...10 3.4 QUANTITY ONE SOFTWARE ...10 3.5 PRINTER (OPTIONAL) ...10 3.6 PACKAGING ...10 SECTION 4 GETTING STARTED ...12 4.1 SELECTING A LOCATION ...12 4.2 ASSEMBLING THE SYSTEM ...12 4.2.1 Assembling the Universal Hood II ...12 4.2.2 Assembling the CCD Camera ...13 4.2.3 Connecting the cabling harnesses...15 4.2.4 Installing the White Light Transilluminator (Optional)...16 4.2.5 Installing the Optional 17 mm or 25 mm Wide-Angle Lenses (ChemiDoc XRS only):...16 4.3 Description of System Function and System Initialization...18 4.3.1 Control panel ...18 4.3.2 System Initialization ...21 4.3.3 Aligning the Camera assembly: ...24 SECTION 5 OPERATION OF THE UNIVERSAL HOOD II...25 5.1 OPERATING THE UNIT ...25 5.1.1 Switch on the Universal Hood II...25 5.1.2 Position your gel ...25 5.1.3 Acquire an image ...25 5.1.4 Acquiring an image with Flat Fielding:...25 5.2 CUTTING GELS ...28 SECTION 6 TROUBLE SHOOTING...29 SECTION 7 ACCESSORIES AND REPLACEMENT PARTS...30 Accessories and Replacement Parts...30 SECTION 8 MAINTENANCE AND PART REPLACEMENT...32 8.1 EPI-ILLUMINATION LAMP REPLACEMENT ...32 8.2 FUSE REPLACEMENT...32 8.3 UV TRANSILLUMINATOR ...33 8.3.1Lamp replacement...33 8.3.2 Starter replacement (P/N 100-1370)...34 APPENDIX A: GEL DOC XR SYSTEM CABLE CONFIGURATION...35 APPENDIX B: CHEMIDOC XRS SYSTEM CABLE CONFIGURATION ...36 APPENDIX C: INSTALLING SOFTWARE AND DRIVERS ...37 Gel Doc XR and ChemiDoc XRS- PC: ...37 Gel Doc XR and ChemiDoc XRS- Mac: ...39

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ALSO READ THE RELEASE NOTES FILE FOR LAST MINUTE UPDATES FOR THE SOFTWARE. ...40 APPENDIX D: PCI CARD INSTALLATION...41 Gel Doc XR – PC:...41 Gel Doc XR - Mac: ...41 ChemiDoc XRS- PC:...41 Installing the PCI Digitizing Card ...41 APPENDIX E: TECHNICAL SPECIFICATIONS ...42

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Section 1 Introduction The Bio-Rad Gel Doc XR, and the ChemiDoc XRS Gel Documentation systems are easyto-use, high-performance systems. They use a CCD Camera to capture images in real time, which allows you to more accurately position and focus the image. While using Bio-Rad Quantity One software, acquired images can be optimized, annotated, analyzed, and printed. The Bio-Rad Gel Doc XR, and the ChemiDoc XRS systems utilize an enclosure (Universal Hood II) that is light tight and contains UV illumination and white light illumination, Please note that both the Gel Doc XR and ChemiDoc XRS Cameras have external power supplies.

1.1 Computer Requirements This software will run under Windows 2000 or XP, or on a Macintosh PowerPC. The amount of computer memory required for using the program is mainly determined by the size of the images you will scan and analyze. Images scanned at high resolution can be quite large. For this reason, we recommend that you archive images on a network file server or removable storage media.

1.2 PC The following is the recommended system configuration for installing and running on a PC: Operating system: Processor: RAM: Hard disk space: Monitor: Printer:

Windows 2000, Windows XP, Intel Pentium 400 MHz or better 128 MB or more for Gel Doc XR, and ChemiDoc XRS systems. 3 GB or greater 17" monitor or better, 1024 x 768 resolution (absolutely required), True color. Optional.

1.3 Macintosh The following is the recommended system configuration for installing and running on a Macintosh: Operating system: Processor/Model: RAM: Hard disk space: Monitor: Printer:

System Mac OS 10 PowerPC G3 processor or better. 256 MB or more for Gel Doc XR, and ChemiDoc XRS systems. 3 GB 17" monitor, 1024 x 768 resolution (absolutely required), Millions of colors. Optional.

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Section 2 Important Safety Information 2.1 Important Notice Use of the Gel Doc XR, and ChemiDoc XRS involves UV illumination. Proper precautions must be taken to avoid eye and skin exposure to the UV radiation. This instrument is meant for use only by specialized personnel that know the health risks associated with UV radiation and the reagents that are normally used with this instrument. The acrylic shield provides some UV protection. However, it does not guarantee complete protection, and it is designed to shield only the person working in front of the system. WARNING: The operator should wear appropriate safety glasses or a protective mask and gloves in addition to using the UV Safety Shield provided with this instrument. Anmerkung: Dieses Geraet ist nur fuer Forschungszwecke geeignet. Ausserdem ist die Benutzung nur fuer spezialisiertes Personal gedacht, das mit den Gesundheitsrisiken vertraut ist, die an die UV-Strahlung gebunden sind und den Reagentien, die normalerweise mit diesem Geraet benutzt werden. Die Benutzung eines Plexiglasschutzschildes garantiert dem Benutzer keinen Schutz vor UVStrahlung. Die Benutzung von Schutzbrille oder Schutzmaske ist strengstens empfohlen.

2.2 Power Safety information a) Voltage Setting Information: The Universal Hood II has a power supply that automatically chooses the correct voltage for your country or region. b) Fusing: The Universal Hood II has two user serviceable fuses. These are located at the following location: •

F1 and F2 are located on the bottom rear panel and are a part of the power entry module, please see picture below for details: Fuse F1 and F2 are located in this drawer. Use a screwdriver and pull the drawer out to see the fuses

Main power entry module

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Section 3 Product Description 3.1 CCD Camera and Lenses A CCD Camera is placed on top of the Universal Hood II for capturing images. The Camera comes with a Motorized Zoom Lens (MZL) that allows a remote adjustment of the lens control functions viz. zoom, focus and iris. For ChemiDoc XRS systems optional lenses with low F-number are available that offer extreme light collection efficiency for low light applications like chemiluminescence. Lens f 0.95, 25 mm, Wide Angle Lens f 0.95, 17 mm, Wide Angle

1708072 1708073

3.2 Darkroom Enclosure, Emission Filters, and Illumination Sources The Universal Hood II is designed to capture fluorescence and chemiluminescence images without using a photographic darkroom. The enclosure has built-in white light epi-illumination and a UV transillumination. An optional White Light Transilluminator (170-7950) or a White Light Conversion Screen (170-8001) may also be used with the system. For easy sample loading, the UV transilluminator is located in the drawer of the Universal Hood II and can accessed from the front of the enclosure. For your convenience, the lights in the darkroom enclosure turn off automatically after about 15 minutes. This time period can be extended indefinitely by pressing the HOLD button. The Universal Hood II has a three position Filter Slider that offers the flexibility of using two different emission filters for fluorescence applications as well as the ability to image chemiluminescence samples (no filter). Bio-Rad Laboratories offers a selection of filters and illumination sources. Standard Filter: 548-630nm

Comes with each system. Amber Filter (Ethidium Bromide)

Optional Filters: 520DF30 nm

Can be purchased for Optimal performance. Optional Filter for SYBR Green/ SYBRGold/ GFP/ Fluorescein 170-8074 Optional Filter for CY3/Rhodamine/Sypro Orange 170-8075 Optional Filter for SYPRO Ruby/Texas Red 170-8076 Optional Filter for Hoechst/Coumarin 170-8077

560DF50 nm 630BP30 nm 480BP70 nm

Optional Illumination Sources: White Light Transilluminator White Light Conversion Screen 302 nm UV lamps 6 ea. (shipped standard with each system) 254 nm UV lamps 6 ea 365 nm UV lamps 6 ea

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170-8081

170-7950 170-8001 170-8097 170-8098 170-6887

3.3 PCI interface Cards Gel Doc XR:

The Gel Doc XR system requires a Fire Wire port to communicate with the computer where the user interface software is installed. In case of computers that do not offer a Fire Wire (IEEE1394) interface, a PCI Fire Wire card (included with the system) needs to be installed in the computer to communicate with the Camera. Note: Read and follow the instructions for installing the PCI card, drivers and Software in Appendix C and D. ChemiDoc XRS:

The ChemiDoc XRS system requires a PCI Digitizing card to communicate with the Camera. This card needs to be installed in all PC and Mac systems with appropriate drivers (included in the software). Note: Read and follow the instructions for installing the PCI card, drivers and Software in Appendix C and D.

3.4 Quantity One Software Each system ships with 1 full version of Quantity One and unlimited copies of Quantity One Basic Mode. The software can be used to annotate and document images, analyze molecular weights, print, and perform a host of other applications. See the software manual for detailed instructions on how to install and operate the software. Note: Read and follow the instructions in Appendix C and D prior to installing Quantity One software.

3.5 Printer (Optional) For your convenience, Bio-Rad offers an optional USB printer for use with the Gel Doc XR and ChemiDoc XRS: Sony UPD895 (recommended for both the systems)

170-8066

3.6 Packaging The Gel Doc XR, or ChemiDoc XRS systems consist of multiple components. Each of them may be in a separate box or in one box with a label describing the catalog numbers and its contents. Please make sure that all the system components are in your shipment. Please unpack each component carefully and verify that you have the correct one. Each system includes the following:

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Part # 170-8170/170-8171 170-8062 170-8172 170-8173 170-8080 170-7964/7963 170-9600 170-8009

Part # 170-8070/170-8071 170-8062 170-8088 170-8080 170-8008 170-7964/7963 170-9600 170-8009

Descriptions Gel Doc XR System PC/Mac Universal Hood II W/TLUM 100/240 Vac Gel Doc XR Camera and Cable w/MZL Gel Doc XR Fire Wire card (PC System only) Kit Accessory, Universal Hood II Cable, Serial, MZL, PC/Mac Quantity One Software PC/Mac Adapter, USB to Serial (Mac System only)

Descriptions ChemiDoc XRS System PC/Mac Universal Hood II W/TLUM 100/240 Vac ChemiDoc XRS Camera W/MZL (including PCI card and Camera Controller Cable) Kit Accessory, Universal Hood II Fluorescent Reference Plate Cable, Serial, MZL, PC/Mac Quantity One Software PC/Mac Adapter, USB to Serial (Mac System only)

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Section 4 Getting Started 4.1 Selecting a Location The Gel Doc XR and ChemiDoc XRS systems can be placed on any bench top. The best position is near the computer where Quantity One will be operating. Remember to leave space to easily access the power switch on the Universal Hood II, which is located at the rear bottom left side of the enclosure

4.2 Assembling the System 4.2.1 Assembling the Universal Hood II Important: It is recommended that the Universal Hood II be carried by at least two people holding the instrument from the bottom side. Be sure that the door and the drawer are completely closed. Wichtig: Es wird empfohlen , dass mindestens zwei Personen den Hood transportieren und dabei das Geraet am Geraeteboden halten.

a) Open the box and review the unpacking instructions. b) Carefully remove all of the components of your system. c) Remove the top foam and the plastic wrapping from the Universal Hood II. d) Carefully pull the enclosure out of the bottom foam and place it in a suitable location. Note: Please do not connect the power cable to a power source until all connections are made. The power source has to be grounded and protected by a circuit breaker.

e) Locate the Amber Filter in the Accessory Kit and thread it onto one of the two 62 mm rings of the Filter Slider (inside of the hood). The Filter Slider facilitates the selection between two filters and an open position for chemiluminescence. f) The three selectable positions are indicated by three symbols O (no filter), I (filter 1) or II (filter 2) on the right side of the Camera support. Note the position of the Amber Filter for future use. See pictures below.

Three positions sliding filter

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4.2.2 Assembling the CCD Camera Gel Doc XR

a) Place the Camera on the bench so that the two tripod mount holes are facing you. b) Using one of the two knurled screws and washers attach the Gel Doc bracket to the Camera as shown in the picture below. Use the tripod mounting hole that is closest to the top of the Camera. Do not tighten the screws yet. c) The black adapter ring provided (3 millimeters thickness) has a soft and a hard side to it. d) Position this adapter plate on top of the Universal Hood II such that the soft side of the ring is facing up and the ring is concentric with the round opening on the universal hood.

Gel Doc  XR Bracket

Gel Doc XR bracket, washers, screws and adapter

Gel Doc XR Camera and bracket

e) Place the Camera over the Universal Hood II by inserting the lens into the adapter ring. f) Make sure that the front of the lens is in direct contact with the black adapter ring. g) Verify that the lens is centered in the opening on top of the enclosure. To do this you may need to move the entire body of the Camera and the ring so that the lens is also in the center of the adapter ring. WARNING: Failing to center the lens in the adapter ring and the hood can cause the focusing ring located on the front side of the lens to mechanically rub or bind.

h) Secure the Gel Doc XR bracket (attached to the Camera) to the bracket of the Universal Hood II using the screw and knurled knob (see pictures below) i) Tighten the two screws of the Gel Doc XR Camera bracket. Check that the lens is well positioned on the black adapter ring for a complete light seal. j) A completely assembled Camera will look like the picture shown below.

Screw, washer and knurled knob

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Gel Doc XR Camera assembled

Upon completion of the Camera installation, refer to Section 4.2.3 and Appendix A for a complete description of the cable connections. ChemiDoc XRS a) Place the Camera on the bench so that the locking hole of the tripod mount is facing you. b) Fix the ChemiDoc XRS bracket to the Camera, do not tighten the screw. c) Place the black adapter ring (6 millimeters thickness) on the top of the hood with its soft side facing up.

Tripod Mount

ChemiDoc XRS Camera with bracket

Black adapter ring for ChemiDoc XRS Camera and its positioning on the top of the Hood

d) Position the ChemiDoc XRS Camera over the opening on the top of the enclosure by inserting the lens through the adapter ring. Make sure that the lens is in close contact with the black adapter ring. Adjust the Camera position such the lens is well centered in the opening of the hood (see below).

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Center the lens in the opening of the hood as viewed from inside of the hood

e) Fix the Camera bracket to the bracket on the Universal Hood II using the screw, washer and knurled knob.

f) Lower the Camera so that the lens fit properly on the gasket of the black adapter ring then tighten the black screw on the tripod mount Upon completion of the Camera installation, refer to Section 4.2.3 and Appendix B for a complete description of the cable connections.

4.2.3 Connecting the cabling harnesses The cable connections are different if you are installing a Gel Doc XR or a ChemiDoc XRS Camera. Please refer to the instructions included in Appendix A, B respectively for Gel Doc XR/ ChemiDoc XRS cable wiring. The controls for the Motorized Zoom Lens can be operated from the membrane touch pad of the Universal Hood II or from the PC using the buttons that appear in the window when the option Gel Doc XR or ChemiDoc XRS is selected in the File Menu of the Quantity One program. To use this option it is necessary to connect the Universal Hood II serial port (DB25) (on the back left side) to the COM1 of the computer. The PC/Mac cable is included.

Note: In the case when a Mac has a USB port instead of the serial port, use the USB to serial adapter (included with Mac systems) to control the lens via software.

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4.2.4 Installing the White Light Transilluminator (Optional) To install the White Light Transilluminator, open the door to the enclosure and pull the drawer completely toward you. Locate the power cable positioned on the left side (inside of the enclosure) behind the slide of the drawer. Remove the black rubber that covers the banana plug and insert it into the White Light Transilluminator outlet. Make sure that the main power switch on the transilluminator is in the ON position. The transilluminator will not illuminate. Power to the White Light Transilluminator is controlled from the membrane touch pad. To use the White Light Transilluminator, place it horizontally on the UV Transilluminator and press the TransWhite button on the membrane touch pad. When the White Light Transilluminator is not in use, store it in the two plastic holders located at the rear of the enclosure.

Storage of the White Light Transilluminator

4.2.5 Installing the Optional 17 mm or 25 mm Wide-Angle Lenses (ChemiDoc XRS only): This installation guide covers installation of the optional lenses for the ChemiDoc XRS system. The following catalog numbers are covered under this category: i. ii.

Lens f 0.95, 25 mm, Wide Angle Lens f 0.95, 17 mm, Wide Angle

The lens kit includes a lens and the Light Seal adapter ring. See pictures below:

25 mm Wide Angle Lens with Light Seal Adapter ring

17 mm Wide Angle Lens with Light Seal Adapter ring

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Setting Up the Lens: 1. Turn off the power to the enclosure and Camera 2. Disconnect the Camera and lens cables 3. Remove the ChemiDoc XRS Camera from the bracket. 4. Remove the existing Motorized Zoom Lens from the Camera by turning the lens counterclockwise. Attach the 25 mm or 17 mm lens to the Camera as shown below:

Remove existing Motorized Zoom Lens

Install the Wide Angle Lens

5. The Wide Angle Lenses comes with a Light Seal that is donut shaped. One side is soft and has a groove around the inner opening. The other is hard. See picture below

Light Seal soft side (Note groove)

Light Seal hard side

6. Position the Light Seal on the top of the hood. Make sure that the “hard side” is against the top of the enclosure. Secure the Camera (with wide-angle lens attached) to the Camera bracket with the black washer and the thumbscrew. Before tightening the screw make sure that the wide-angle lens is positioned in the round slot in the Light Seal (soft side up). Carefully push the Camera down so that the lens goes into the slot all the way and comes to a stop. See pictures below:

Make sure that the lens is seated properly in round slot in the soft side of the Light Seal

7. Adjust the position of the bracket so that the Light Seal and lens are well centered in the round opening in the Universal Hood II and tighten the screws to secure the Camera tightly to the hood. 8. The Wide Angle lenses have an Iris and Focus ring that allows users to adjust for optimum light collection and focal plane. See image on the next page.

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Focusing Ring

Iris Control Ring

9. For imaging of chemiluminescent samples, make sure that the Iris is opened all the way.

4.3 Description of System Function and System Initialization 4.3.1 Control panel The front membrane touch pad control panel of the Universal Hood II allows full control of the Motorized Zoom Lens, UV transillumination, White Epi illumination and White Light transillumination. Please read the following section to become familiar with each function.

Power Indicator LED Imaging Indicator LED

Trans UV LED and button Prep UV LED and button Epi-White LED and button Trans White LED and button Hold LED and button

Membrane Touch Pad

The Power light tells you whether the system is turned on or not. The power on/off switch is located at the rear bottom left side of the enclosure.

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The Imaging led blinks when you are acquiring an image with the Camera (this function is not present using the Gel Doc XR or ChemiDoc XRS Cameras) indicating integration by the Camera. It also blinks if the computer is turned off.

Light Sources The Epi-White button controls the Epi White illumination. Press the button to turn ON EpiWhite; press the button again to turn it OFF. Epi illumination will automatically turn off after 15 minutes, unless the Hold button is activated. When the UV transilluminator switch is activated Epi-White is automatically turned off. The White Light Trans button controls the White Light Transilluminator when installed (optional). It will automatically turn off after 15 minutes, unless the Hold button is activated. The UV Trans button controls the UV Transilluminator with full intensity. The transilluminator will automatically turn off after 15 minutes unless the Hold button is activated. For safety purposes, this switch is subject to the following automatic controls: • •

It is automatically turned off when the front door is open. It is automatically turned off when the drawer is open.

If the door or drawer is opened while the transilluminator is ON, the blinking LED will warn you that the transilluminator has been turned OFF. After the door or the drawer is closed, you have to press the UV Trans switch again to turn it ON.

Important: For the purposes of band cut applications, it is possible to turn ON the UV light with the drawer fully open. You must wear all possible UV protections, especially for your eyes, when the transilluminator switch is turned ON with the drawer fully open. The UV shield has to be used but glass or mask and gloves are recommended to block the UV radiations. This option is not applicable to the door. Wichtig: Wer die Absicht hat, Banden aus dem Gel zu schneiden, kann bei eingeschaltetem UV-Licht die untere Schublade oeffnen. Sie muessen Schutzkleidung und die notwendigen UV-Schutzmassnahmen tragen, besonders Augenschutz, wenn der transilluminator bei offener Schublade eingeschaltet ist. Das Plexiglasschutzschild muss benutzt werden, ebenso Schutzbrille oder Schutzmaske und Einmalhandschuhe, um sich vor der UV-Strahlung zu schuetzen. Diese Moeglichkeit ist von der Geraetetuer aus nicht anwendbar. The UV Prep switch is used to decrease the light output of the UV Transilluminator. The UV Preparative function is a lower intensity light designed to minimize the effects of UV exposure on DNA. The Preparative mode is recommended for applications that require longer UV exposure times. In order to activate the UV Prep switch, the UV Trans button must be on. The Hold key disables the automatic shut-off of the UV and White light transilluminators and the Epi-illumination; the lights will remain active until the hold status is cancelled. When the Hold key is pressed, the orange LED lights, indicating that the Hold function is active.

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If opening the door or drawer (activating the UV interlock, which turns off the UV), you will need to turn off the Hold button before pressing any other switch. The control panel has a second section that includes the buttons to run all the MZL functions (see the Lens Control Section below).

Camera Lens and Filters The MZL functions are operated from the membrane touch pad or through the acquisition window in the software. To control the lens through the Quantity One acquisition window, it is necessary to connect the Universal Hood II with a null modem cable (serial cable) from the COM1 port on your computer to the serial port of the hood itself. A serial cable is supplied with each instrument. If the only port present is the USB port then it is necessary to use a USB To Serial converter (Part # 170-8009) for PC and for Mac (all Mac systems ship with a converter).

Lens Control The membrane pad has a Fast and a Slow button to obtain rapid or fine adjustments of the lens. An image can be optimized with the following lens adjustments:

Aperture is adjusted using the “Iris” buttons on the membrane touch pad. The size of the aperture opening allows the CCD sensor to take in more or less light. The Iris has two buttons: One button, Close (-), reduces the size of the aperture decreasing the amount of light coming into the sensor, thereby making the image darker. The other button, Open (+), opens the aperture increasing the amount of light coming into the sensor, resulting in a brighter image. Focus is adjusted using the “Focus” buttons on the keypad. Pushing the Near (+) button or the Far (-) button changes the focal point of the lens and allows the user to focus on the sample. Zoom is adjusted using the “Zoom” buttons on the keypad. It allows you to change the size of the image on the screen. Pushing the Wide (-) button zooms out (far field). Pushing the Tele (+) button zooms in (close up).

Membrane touch pad – Lens Control Section

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Filters: A +1 Diopter is factory installed on the lens. This Diopter should always remain on the lens assembly. An Amber Filter is included in the Accessory Kit. Place the Amber filter in position 1 or 2 of the Filter Slider (See 4.2.1). The Amber filter should be used for fluorescence (UV) and colorimetric (White light) applications.

4.3.2 System Initialization Before starting the initial test please make sure that: 1. The software and the digitizing board are installed properly (See Appendix C and D). 2. The cabling harness is connected properly. (See Appendix A or B) Please follow the procedure in the table to ensure that the Universal Hood II is functioning properly.

STEP 1: Initial test for the Universal Hood II: Procedure Control Panel Make sure the door and drawer are closed and the computer is switched on. Turn on the system. Power LED turns on after short blinking. Wait for 30 minutes for Camera to Nothing will change except the Camera will warm warm up up. Press the Epi-White key. Epi-White LED turns on. Open the door. Epi-lights are on, Trans UV LED blinks. Press the Epi-White key again. Epi-lights and LED are turned off. Close the door. Trans UV LED turns off. Press the Trans UV key. Trans UV LED turns on. Open the door. Trans UV LED blinks. Close the door. Trans UV LED turns off. Press the Trans UV key. Trans UV LED turns on. Open the drawer. Trans UV LED blinks. Close the drawer. Trans UV LED turns off. Press the Trans UV key. Trans UV LED turns on. Press the Prep UV key Prep UV LED turns on. Press the Hold key. Hold LED turns on. Open the drawer. Trans UV LED blinks, Prep UV LED and Hold LED turns off. Close the drawer. Trans UV LED turns off. Note: The Light Source buttons on the control panel are disabled when the Imaging LED blinks (indicating that the computer is switched Off).

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STEP 2: Initialization of the Camera and lens assembly Procedure (for Gel Doc XR/ ChemiDoc XRS systems): 1. Press the Epi-Illumination button. 2. Open the door and determine if lights are on. 3. Place the focusing target on the UV transilluminator. 4. Double click on Quantity One software icon on the desktop. From “File Menu” select “Gel Doc XR” or “ChemiDoc XRS”.

NOTE: Dark Reference comes up on first use for both Gel Doc XR and ChemiDoc XRS systems. Please refer to next step for details about “Reference Dark” image acquisition. 5. Select Live/Focus. 6. An image of the target will appear on the computer screen. Using the lens control buttons on the membrane touch pad or the control panel that appears in the software, find the best Iris, Focus, and Zoom conditions.

Note: Working with a widely open Iris and changing the Zoom may require you to adjust the Focus. STEP 3: Dark Reference Acquisition: Note: Please note that the steps described below must be performed in either of the following scenarios: a. When installing the system with a new computer or Camera b. When software is first installed or reinstalled for any reason

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