Cepheid
SmartCycler Dx Operator Manual v3.0 Rev C
Operator Manual
182 Pages
![Table of Contents Preface: Safety Information... 1 Chapter 1: Theory of Operation... 7 1.1 Introduction... 8 1.2 Heating/Cooling... 8 1.3 Optical System... 9 1.4 Reaction Tube... 10 1.5 Calibration...11 Chapter 2: Installation... 13 2.1 Installing the SmartCycler Dx System... 15 2.2 Installing additional SmartCycler Processing Blocks... 17 2.3 Opening the SmartCycler Dx Software... 19 2.4 Closing the SmartCycler Software...20 2.5 Uninstalling or reinstalling SmartCycler Dx Software... 21 2.6 Using the SmartCycler Reaction Tubes... 21 2.7 Installation qualification...24 2.8 Defining users and permissions (Administrator)... 25 2.9 Password Management... 31 2.10 Logging on and off... 31 2.11 Configuring the system... 32 Chapter 3: IVD Assays... 35 3.1 Introduction... 37 3.2 Definitions... 37 3.3 Importing IVD Assays... 38 3.4 IVD Assay Run Setup... 39 3.5 Adding and Removing Selected Sites...44 3.6 Cancelling Run Setup...45 3.7 Viewing Run Results...45 3.8 IVD Assay Troubleshooting... 56 SmartCycler Dx Operator Manual 300-5010 Rev. C iii](https://images.bioclinicalservices.com.au/r5ujDXg1zDKTCd7jYzrUvVd9/320w/Cepheid%20-%20SmartCycler%20Dx%20Operator%20Manual%20v3.0%20Rev%20C.png)
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Dx Operator Manual SmartCycler® v3.0
SmartCycler Dx Operator Manual 300-5010 Rev. C
Warranty The following information constitutes the Product-specific warranties referenced in the purchase agreement (typically bearing a title such as “Purchase Agreement” or “Sales Agreement”) under which the SmartCycler® Instrument and Software described herein were purchased from Cepheid®. Purchaser must not alter or remove any labels, signs, symbols, serial numbers, copyright, patent, trademark, trade secret, proprietary and/or other legal notices contained on or in this Manual, the SmartCycler® Instrument, SmartCycler Software, and related documentation.
SmartCycler Instrument Limited Warranty Cepheid warrants that (i) the SmartCycler Instrument (the “Instrument”) is free from defects in material and workmanship, (ii) the Instrument together with the SmartCycler Dx System Software (the “Product”) conforms to Cepheid's published specifications, and (iii) the Product conforms to the labeling claims that accompany the Instrument. This Warranty is for a period of 12 months from the date of shipment to the Purchaser (the Warranty Period). During the Warranty Period, if the Instrument's hardware is found to be defective or if the Product is found to be non-conforming under item (ii) or (iii) above, Cepheid will repair or replace it, at a site determined by Cepheid at Cepheid's expense. This warranty extends to Purchaser only and not to any other parties, except as agreed to in writing by Cepheid, and applies only to new products manufactured by Cepheid. Cepheid does not warrant any defects in the Instrument caused by (i) improper installation, removal or testing, (ii) Purchaser's failure to provide a suitable operating environment for the Instrument, (iii) use of the Instrument for purposes other than that for which it was designed, (iv) unauthorized attachments, (v) unusual physical or electrical stress, (vi) modifications or repairs done by other than Cepheid or a Cepheid authorized service provider, or (vii) any other abuse, misuse, or neglect of the Instrument. The Instrument is designed and certified with applicable regulatory authorities as part of an integrated instrument/reagent/consumable system. Use of unapproved parts, reagents or other materials with the Instrument will void any warranty and any service contract between Cepheid and the Purchaser that pertains to the Instrument. OTHER THAN ANY EXPRESS WARRANTY PROVIDED IN THE ORIGINAL PURCHASE AGREEMENT WITH CEPHEID, THERE ARE NO WARRANTIES WHICH EXTEND BEYOND THE FACE HEREOF, AND CEPHEID DISCLAIMS ALL OTHER REPRESENTATIONS AND WARRANTIES, WHETHER EXPRESS OR IMPLIED, REGARDING THE PRODUCT, INCLUDING ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. CEPHEID AND ITS DIRECTORS, OFFICERS, EMPLOYEES AND AGENTS SHALL HAVE NO LIABILITY FOR GENERAL, CONSEQUENTIAL, INCIDENTAL OR SPECIAL DAMAGES ARISING FROM A DEFECT IN THE INSTRUMENT. FURTHERMORE, CEPHEID SHALL HAVE NO STRICT LIABILITY, PRODUCTS LIABILITY OR NEGLIGENCE LIABILITY, WHETHER ACTIVE OR PASSIVE, AS TO THE PURCHASER. Purchaser's exclusive remedy for any defective Instrument or non-conforming Product is limited to the repair or replacement of any defective Instrument. If Cepheid cannot or does not repair or replace a defective Instrument or nonconforming Product, Cepheid will remove same and return the purchase price. If Cepheid cannot or does not repair or replace any defective Instrument or non-conforming Product or if Purchaser's exclusive remedy fails of its essential purpose, Cepheid's entire liability shall in no event exceed the purchase price for any defective Instrument or non-conforming Product.
Cepheid SmartCycler Dx System Software License Agreement Cepheid is the exclusive owner of the Cepheid SmartCycler Dx System Software program (the "Software"), related documentation and physical media, and of all copyright, trade secret, patent, trademark and other intellectual or industrial property rights therein. Physical media and copies of the Software, whether in diskette, tape, paper or other form, shall remain the property of Cepheid, and such copies are deemed to be on loan to Purchaser during the term of the License granted hereby. Purchaser expressly acknowledges that no title to or ownership of the Software, or any copy or portion thereof, is transferred to Purchaser. The ideas and expressions thereof contained in the Software are confidential, proprietary information and trade secrets of Cepheid that are provided to Purchaser. Purchaser shall not cause or permit decompilation, disassembly, or reverse engineering of the Software or disclosure, copying, display, loan, publication, transfer of possession (whether by sales, exchange, gift, operation of law or otherwise) or other dissemination of the Software and related documentation, in whole or part, to any third party without the prior written consent of Cepheid. License Grant: Cepheid grants Purchaser a non-exclusive, non-transferable license (the "License") to use only one (1) copy of the Software on the computer provided by Cepheid with the SmartCycler Instrument(s) and connected to the SmartCycler Instrument(s), and to make only one (1) copy solely for back-up purposes. Purchaser shall not otherwise copy and shall not modify, duplicate, translate, disassemble, or decompile the Software without Cepheid's prior written
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consent. If the Software is used on a computer other than the one provided by Cepheid for the SmartCycler Instrument, Cepheid will not guarantee performance and cannot provide technical support for problems arising therefrom. Purchaser may not use this single-user product on a network. This License entitles Purchaser to use toll-free telephone support as provided by Cepheid. This License is effective until terminated. Cepheid may terminate this License if Purchaser fails to comply with any of the terms or conditions of this License or of the original purchase agreement. If this License is terminated, Purchaser must destroy all copies of the Software and its related documentation. For Government Purchasers, the Software is commercial computer software subject to restricted rights under FAR 52.227-19 (C) (1, 2).
Patent Statements NOTICE TO PURCHASER This real-time thermal cycler is licensed under U.S. Patent No. 6,814,934 and corresponding claims in any foreign counterpart patent thereof owned by Applera Corporation, for use in research and development and all other applied fields including human in vitro diagnostics. No rights are conveyed expressly, by implication or estoppel to any patents on real-time methods, including but not limited to 5' nuclease assays, or to any patent claiming a reagent or kit. For further information, contact the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA. Licensed under all applicable claims of U.S. Patent Nos. 5,589,136, 6,524,532, and 6,602,473, and corresponding claims in their non-U.S. counterparts. The purchase of this instrument includes a limited, non-transferable license under U.S. Patents Nos. 6,787,338; 6,503,720 and 6,303,305, and claims 9, 10, 11, 56, 76, 80 and 107 of U.S. Patent No. 6,174,670, and corresponding claims in patents and patent applications outside the United States, owned by the University of Utah Research Foundation and licensed to Idaho Technology, Inc. No right is conveyed, expressly, by implication or estoppel, under any other patent or patent claims owned by the University of Utah Research Foundation or Idaho Technology, Inc. Without limiting the foregoing, no right, title or license is herein granted with respect to the uses that are proprietary to Idaho Technology or the University of Utah Research Foundation of fluorescent double stranded nucleic acid binding dyes, specifically including but not limited to SYBR® Green I, LCGreen® I, or LCGreen® Plus. The purchase of this product alone does not imply any license under patents owned by Roche Molecular Systems, Inc. or F. Hoffmann-La Roche Ltd.
Trademark and Copyright Statements Cepheid®, the Cepheid logo, SmartCycler® and I-CORE® are trademarks of Cepheid. Adobe® and Acrobat® are registered trademarks of Adobe Systems Incorporated. Alexa Fluor® and Texas Red® are registered trademarks of Molecular Probes, Inc. Microsoft® and Windows® are registered trademarks of Microsoft Corporation. All other trademarks are the property of their respective owners. This Manual contains information protected by copyright. No part of this Manual may be photocopied or reproduced in any form without prior written consent from Cepheid.
© Cepheid 2009 - All rights reserved.
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SmartCycler Dx Operator Manual 300-5010 Rev. C
Table of Contents Preface: Safety Information... 1 Chapter 1: Theory of Operation... 7 1.1 Introduction... 8 1.2 Heating/Cooling... 8 1.3 Optical System... 9 1.4 Reaction Tube... 10 1.5 Calibration...11
Chapter 2: Installation... 13 2.1 Installing the SmartCycler Dx System... 15 2.2 Installing additional SmartCycler Processing Blocks... 17 2.3 Opening the SmartCycler Dx Software... 19 2.4 Closing the SmartCycler Software...20 2.5 Uninstalling or reinstalling SmartCycler Dx Software... 21 2.6 Using the SmartCycler Reaction Tubes... 21 2.7 Installation qualification...24 2.8 Defining users and permissions (Administrator)... 25 2.9 Password Management... 31 2.10 Logging on and off... 31 2.11 Configuring the system... 32
Chapter 3: IVD Assays... 35 3.1 Introduction... 37 3.2 Definitions... 37 3.3 Importing IVD Assays... 38 3.4 IVD Assay Run Setup... 39 3.5 Adding and Removing Selected Sites...44 3.6 Cancelling Run Setup...45 3.7 Viewing Run Results...45 3.8 IVD Assay Troubleshooting... 56
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Table of Contents
Chapter 4: Research and Reference Assays... 59 4.1 Introduction... 61 4.2 Definitions... 61 4.3 Background Subtraction... 65 4.4 Cycle Threshold...68 4.5 Cycle Threshold Setting...68 4.6 Curve Analysis Settings...69 4.7 Melt Analysis...70 4.8 Define Assays Screen... 71 4.9 Defining a New Research Assay... 72 4.10 Thermal Cycling Protocols... 73 4.11 Analysis Settings... 77 4.12 Manual, Run-based Auto, and Site-based Auto Thresholds... 81 4.13 Internal Control Settings...84 4.14 Customizing Result Text... 85 4.15 Control Settings... 91 4.16 Probe Check Settings... 93 4.17 Advance to New Stage...94 4.18 Standard Curve (Quantitative Assay)...96 4.19 Advanced Settings... 100 4.20 Defining a Reference Assay... 100 4.21 The Create Run Screen... 102 4.22 The View Results screen... 107 4.23 Selecting graphs to associate with a run...117 4.24 Exporting Data... 118 4.25 Printing a Run Report... 120 4.26 The Define Graphs Screen...121 4.27 The Check Status Screen... 128 4.28 Stop Run... 128 4.29 The Maintenance Screen... 129
Chapter 5: Logs, Reports and Data Management... 131 5.1 Generating Logs and Reports... 133 5.2 Data Management... 140 5.3 Getting Help... 145
Chapter 6: Maintenance...147 6.1 Removing and Replacing I-CORE® Modules... 149 6.2 I-CORE Calibration... 154 6.3 Disinfecting the SmartCycler Processing Blocks and I-CORE Modules... 154 6.4 Disinfecting the Tube Rack and Cooling Block... 155
Chapter 7: Troubleshooting... 157 Appendix A: Windows 2000/XP with SmartCycler Dx Software... 169 Appendix B: SmartCycler Technical Specifications... 171 Index... 173 iv
SmartCycler Dx Operator Manual 300-5010 Rev. C
Caution: This heading and symbol are used to indicate that non-compliance with instructions or procedures may cause damage to the instrument. Vorsicht: Dieser Kopftext und Symbol werden benutzt, um anzuzeigen, daß Zuwiderhandlung gegen Anweisungen oder Prozeduren Beschädigung des Instrumentes verursachen kann.
Note: This heading is used to bring your attention to topics of importance. Safety Labels There are two safety labels on the SmartCycler instrument. The labels contain symbols that indicate the type of potential hazard, as well as signal words accompanied by definitions of the particular hazard. Es gibt zwei Sicherheit Kennsätze auf dem SmartCycler-Instrument. Die Kennsätze enthalten Symbole, die die Art der möglichen Gefahr anzeigen, sowie die Kennzeichenwörter, die von den Definitionen der bestimmten Gefahr begleitet werden. This safety label below is located inside the SmartCycler lid. Dieser Sicherheit Kennsatz befindet sich innerhalb der Kappe des SmartCycler.
The safety label below is located on the back panel of the SmartCycler instrument. Dieser Sicherheit Kennsatz ist auf der Rückplatte des SmartCycler.
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Note on Use with Infectious Material Anmerkung über Gebrauch mit Ansteckendem Material The instrument may not be used to analyze infectious materials, unless additional safety measures to ensure safe sample handling (e.g., placing the instrument in a laminar flow biological safety cabinet) are taken beforehand. Das Instrument darf nicht benutzt werden, um ansteckende Materialien zu analysieren, es sei denn zusätzliche Sicherheitsmaßnahmen die sichere Probebehandlung sicherzustellen (z.B., das Instrument in einen blätterigen Luftstrom biologischen-Sicherheit Schrank legend), vorher genommen werden.
Marks of Conformity Markierungen der Übereinstimmung The SmartCycler instrument has been manufactured according to EN 61010-1 (Safety Regulations for Measuring, Control, and Laboratory Instruments); Part 1: General Requirements [IEC 1010-1 + A1: 1992, modified]). The SmartCycler has been checked in accordance with all relevant safety standards before leaving the factory. Das SmartCycler-Instrument ist hergestellt worden entsprechend en 61010-1 (Sicherheit Regelungen für Messen, Steuer- und Laborinstrumente); Teil 1: Allgemeine Anforderungen [IEC 1010-1 + A1: 1992, geändert]. Das SmartCycler ist in Übereinstimmung mit allen relevanten Sicherheit Standards überprüft worden, bevor man die Fabrik verließ.
Electrical Symbols Elektrische Symbole The symbols shown below are affixed to the SmartCycler. Read the explanations and make sure you understand what the symbols mean before you interact with the instrument in any way. Die Symbole, die auf dieser Seite gezeigt werden, werden zum SmartCycler hinzugefügt. Lesen Sie die Erklärungen und verstehen was die Symbole bedeuten bevor Sie auf das Instrument in jeder Hinsicht einwirken. This symbol indicates the on position of the main power switch. Dieses Symbol zeigt die Arbeitsstellung des Betriebsschalters an.
This symbol indicates the off position of the main power switch. Dieses Symbol zeigt die Ausschaltstellung des Betriebsschalters an.
This symbol indicates that a protective grounding terminal must be connected to earth ground before any other electrical connections are made to the instrument. Dieses Symbol zeigt an, daß ein Schutzleiteranschluß an Masse Boden angeschlossen werden muß, bevor alle anderen elektrischen Beziehungen zum Instrument hergestellt werden.
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SmartCycler Dx Operator Manual 300-5010 Rev. C
This symbol indicates that this terminal either receives or delivers alternating current or voltage. Dieses Symbol zeigt an, daß dieses Terminal entweder empfängt oder liefert Wechselstrom oder Spannung.
Contact Information Cepheid 904 Caribbean Drive Sunnyvale, CA 94089-1189 Tel: (408) 541-4191 Fax: (408) 541-4192 Technical Support toll-free: (888) 838-3222 Option 2 6:00 am to 5:00 pm, Pacific time, Monday–Friday) Email: [email protected]
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Chapter 1: Theory of Operation 1.1 1.2 1.3 1.4 1.5
Introduction... 8 Heating/Cooling... 8 Optical System... 9 Reaction Tube... 10 Calibration... 11
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Chapter 1: Theory of Operation
1.1
Introduction
The Cepheid SmartCycler System is an integrated DNA/RNA amplification and detection instrument system based on the proprietary microprocessor-controlled I-CORE® (Intelligent Cooling/Heating Optical Reaction) module. Ease of use is designed into the system through the SmartCycler Dx Software. Each SmartCycler processing block contains sixteen independently controlled, programmable I-CORE modules, each with one reaction site. Thermally optimized proprietary reaction tubes combined with the unique design of the I-CORE modules allow very rapid cycling and faster amplification and detection. Up to six SmartCycler processing blocks can be daisy-chained together, allowing simultaneous analysis of up to 96 discrete samples. The SmartCycler Dx System is suited to in vitro diagnostic assays that require automatic, repeated, rapid heating and cooling cycles for test samples, such as PCR and RT-PCR. Specific sequences can be amplified and detected. The system has the capacity to store any number of runs, limited only by available disk space. All assay data and results are stored in a database. Reaction tube
Heater Fan
Emission detection optical block
Excitation optical block I-CORE circuit board
Notch
Figure 1-1: Schematic diagram of the major components of an I-CORE module.
1.2 Heating/Cooling The disposable reaction tube is inserted into the I-CORE thermal cycling module for amplification. The chamber consists of two heater plates made of a ceramic material that has high thermal conductivity to assure temperature uniformity and rapid heat transfer. Resistive heater elements are deposited on the ceramic plates using thick film technologies and a thermistor, attached directly to each plate, monitors its temperature. Cooling is accomplished with a high-efficiency fan that moves ambient air across the heater plates. The thermal cycling chamber's temperature is controlled by the instrument's firmware. The firmware incorporates a control loop to ensure rapid heating of the plates and to control temperature overshoot around the desired point, allowing the temperature of the fluid in the SmartCycler reaction tube to be changed rapidly and precisely.
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Optical System
1.3 Optical System The SmartCycler optical system uses high intensity light-emitting diodes (LEDs), silicon photodetectors, and appropriate filters for excitation and detection of four different spectral bands. The optical system includes two optical blocks: (Figure 1-2), a four-color excitor module and, a four-color detector module. These blocks are positioned within the device such that their apertures mate with the optical windows of the SmartCycler reaction tube, allowing excitation and emission detection of the reaction mixture. Excitation Optical Block
Reaction tube Lens
LED
Filters
Lens
Mirror
Emission Detection Optical Block
Reaction tube
Lens
Mirror
Filters
Detector
Figure 1-2: Schematic diagram of the SmartCycler optical blocks.
The optical system has four optical channels. The excitation and detection spectral bands for the four channels using the standard filter set for FAM™, TET™, Cy™3, Alexa Fluor® 532, Texas Red®, Cy™5 and Alexa Fluor® 647 are as follows:
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Chapter 1: Theory of Operation
Optical Channel
Excitation (nm)
Emission (nm)
Calibrated Dye(s)
1
450–495
510–527
FAM, Intercalating Dye
2
500–550
565–590
Cy3, TET Alexa Fluor 532
3
565–590
606–650
Texas Red
4
630–650
670–750
Cy5 Alexa Fluor 647
Table 1–1: Excitation and detection ranges for SmartCycler optical channels.
By using probes labeled with different fluorescent dyes, up to four dyes and targets can be amplified and detected simultaneously in a single reaction mixture. The optical system always collects data from all four channels. Because the emission spectra of fluorescent dyes can overlap, appropriate calibration and data analysis algorithms are used to separate the signal from each of the dyes.
1.4 Reaction Tube The unique SmartCycler polypropylene reaction tube (Figure 1-3) is designed for optimal thermal and optical characteristics. The thin SmartCycler reaction tube allows rapid rates of heating and cooling of the reaction mixture, and hence rapid amplification. The SmartCycler reaction tube also includes two optical excitation/detection windows at a 90° angle to each other along the bottom edges of the tube. These optical windows interface with the I-CORE optical blocks to allow fluorescence excitation and emission detection to occur.
Cap front
Cap back
Optical excitation/detection window
Optical excitation/detection window
Figure 1-3: SmartCycler 25 μL reaction tube.
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Calibration
1.5 Calibration The thermistors used to monitor the reaction chamber temperature are calibrated to ±0.50 °C using National Institute of Standards and Technology (NIST)-traceable standards. During the manufacturing process, the temperature of the heating system is measured at two temperatures, 60 °C and 95 °C. Calibration coefficients which correct for small errors in the raw thermistor readings of the heaters are stored in the memory of each I-CORE module. The optical system is calibrated using standard concentrations of the individual unquenched dyeoligos to be detected. For each optical channel, the signal produced by buffer alone (the blank signal) is subtracted from the raw signal produced by the dye-oligo standard to determine the spectral characteristics. Using the individual spectral characteristics of the pure dye-oligos, signals from an unknown mixture of dye-oligos can be resolved into corrected signals for the individual dye-oligos in the mixture.
Important: The recommended I-CORE® module calibration interval is 1000 runs or one year of use, whichever occurs first. Please contact Cepheid Service to schedule recalibration for your instrument.
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Chapter 1: Theory of Operation
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SmartCycler Dx Operator Manual 300-5010 Rev. C
Chapter 2: Installation 2.1
Installing the SmartCycler Dx System... 15 • Setting up the SmartCycler Computer... 15 • Setting up the SmartCycler Processing Block... 16 2.2 Installing additional SmartCycler Processing Blocks... 17 2.3 Opening the SmartCycler Dx Software... 19 2.4 Closing the SmartCycler Software... 20 2.5 Uninstalling or reinstalling SmartCycler Dx Software... 21 2.6 Using the SmartCycler Reaction Tubes... 21 • Handling the Reaction Tubes... 21 • Using the tube puller... 22 • Using the Tube Rack and Cooling Block... 23 2.7 Installation qualification... 24 2.8 Defining users and permissions (Administrator)... 25 • User types... 26 • Specifying user permissions... 27 • Managing users... 28 • Adding new users... 28 • Editing user profiles... 29 • Removing users... 30 2.9 Password Management... 31 • Changing your password... 31 • Forgotten passwords... 31 2.10 Logging on and off... 31 2.11 Configuring the system... 32 • System Configuration... 33 • Auto Export Configuration... 34
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Chapter 2: Installation
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Installing the SmartCycler Dx System
2.1 Installing the SmartCycler Dx System Setting up the SmartCycler Computer Notes: The SmartCycler Dx Software is pre-installed on the SmartCycler computer. Do not install other software programs on the SmartCycler computer. If other programs are installed on the computer they may interfere with the SmartCycler Dx Software and data loss may occur. Do not connect additional equipment to the SmartCycler computer by a USB connection, for example, USB hub or Zip® drive. Set up the SmartCycler Dx System on a level, hard surface. Multiple processing blocks should be placed at least 2 inches apart for efficient cooling. Do not cover or obstruct air vent holes on the sides of the processing blocks. Processing blocks can be placed adjacent to each other but be careful not to block the airflow between them (for example, with folders or papers).
Important: Do not install other software programs on the SmartCycler computer. If you install other programs on the computer they can interfere with the SmartCycler Dx Software and data loss can occur. 1. Set up the computer as directed in the manufacturer's instructions. 2. Turn on the computer.
Important: If a user name other than Administrator (Windows 2000) or Cepheid (Windows XP) is used at log in, the power management settings will be incorrect. Set all of the power management settings to Never for every user. Windows 2000: select Start > Settings > Control Panel > Power Options. Windows XP: select Control Panel > Power Options. 3. Confirm that the SmartCycler Dx Software icon is on the computer desktop (Figure 2-1). If the icon is present, the software has been installed successfully.
Figure 2-1: The SmartCycler Dx Software icon.
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Chapter 2: Installation
Setting up the SmartCycler Processing Block 1. Unpack the SmartCycler processing block. The following should be included: 1 SmartCycler processing block 1 power cord 1 USB cable
1 cooling block 4 tube racks 2 tube pullers
2. Connect the power cord to the SmartCycler processing block and into a surge protector power strip or into a power outlet.
Note: The SmartCycler processing block power requirements are 100–240VAC, 50–60Hz.
Note: The acceptable operating temperature range for the SmartCycler Dx System is 15–35 °C. 3. Insert the flat end of the USB cable into the connector labeled with the USB icon on the back of the computer as shown in Figure 2-2. Insert the square end of the USB cable into the square USB connector on the back panel of the SmartCycler processing block.
Computer
SmartCycler processing block
To monitor To AC outlet
USB cable To mouse
To AC outlet
Figure 2-2: Connecting the first SmartCycler processing block to the computer via a USB cable.
4. Turn on the SmartCycler processing block. 5. Double-click on the SmartCycler icon on the Windows desktop (Figure 2-1) or, from the Windows Start menu select Programs > Cepheid > SmartCycler Dx. 6. The SmartCycler Dx splash screen will appear on the computer, and the letter “A” will light up and momentarily flash in the small round display areas on the top left side of the SmartCycler processing blocks. The flashing “A” indicates that the firmware is downloading from the computer to the processing block. After a short time, the View Results screen will be displayed.
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SmartCycler Dx Operator Manual 300-5010 Rev. C
Installing additional SmartCycler Processing Blocks
7. The first time the SmartCycler Dx Software is opened the user will not be asked to enter a user name and password. Some assays will require that user administration to be activated and that all users log in to the SmartCycler Dx software to create and start runs. See “Defining users and permissions (Administrator)” on page 25 for detailed information. 8. To shut down the SmartCycler Dx Software, choose Exit from the User menu, or click the close box in the top right corner of the SmartCycler Dx Software window.
2.2 Installing additional SmartCycler Processing Blocks Note: Windows® 2000/XP users must be logged in as an administrator to install additional processing blocks. For Windows 2000 the user name is Administrator and the Password field is left blank. For Windows XP the user name is Cepheid and the Password is CPHD. 1. Unpack the additional SmartCycler processing blocks, power cords and USB cables. Set up the SmartCycler processing blocks on a level hard surface at least 2 inches apart on all sides for efficient cooling. Do not cover or obstruct air vent holes of the processing blocks. Processing blocks can be placed adjacent to each other but be careful not to block air flow between them, for example, with folders or papers. 2. Close the SmartCycler Dx Software. 3. Turn the previously installed SmartCycler processing block’s power off. 4. Connect the power cord to the second SmartCycler processing block and into a surge protector power strip or into a power outlet (Figure 2-3). First SmartCycler processing block (1)
Second SmartCycler processing block (2)
Computer
USB cable
To monitor To AC outlet
To mouse To AC outlet
To AC outlet
Figure 2-3: Connect the power cord to the second SmartCycler processing block and into a power outlet.
5. Insert the flat end of the USB cable into the second connector labeled with the USB icon as shown in Figure 2-4. Insert the square end of the USB cable into the square USB connector on the back panel of the second SmartCycler processing block.
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