Fisher Scientific
Fisherbrand Centrifuges
Fisherbrand GT1 and GT1R Centrifuge Instruction Manual Rev b Nov 2020
Instruction Manual
39 Pages
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Instruction manual
Fisherbrand GT1 Centrifuge Fisherbrand GT1R Centrifuge 50156465-b • 11 / 2020 15878722 15868722
IMPORTANT Read this instruction manual. Failure to follow the instructions in this manual can result in damage to the unit, injury to operating personnel and poor equipment performance. CAUTION All internal adjustments and maintenance must be performed by qualified service personnel. Material in this manual is for informational purposes only. The contents and the product it describes are subject to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this manual. In no event shall be held liable for any damages, direct or incidental, arising from or related to the use of this manual. HIGHConic™, CLINIConic™, MicroClick™ and Auto-Lock™. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise specified.
© 2020 Thermo Fisher Scientific Inc. All rights reserved.
Table of Contents Preface ... 1 Items Supplied ... 1 Intended Use... 1 Precautions ... 1 Symbols used on the Centrifuge... 2 Technical Specifications... 3 Technical Data ... 3 Directives, Standards and Guidelines ... 4 Mains Supply ... 4 Refrigerant ... 4 Rotor Selection ... 5 Transport and Set Up ... 6 Before Setting Up... 6 Location ... 6 Transporting... 6 Leveling... 6 Mains Connection ... 7 Storage ... 7 Shipping... 7 Control Panel ... 8 Control Panel ... 8 Keys... 8 Operation... 9 Switching on the Centrifuge ... 9 Open the Centrifuge Door ... 9 Close the Centrifuge Door ... 9
Rotor Installation ... 9 Acceleration / Deceleration Rates... 10 Programs ... 12 Centrifugation... 12 Short-term Centrifugation... 13 Removing the Rotor ... 13 Aerosol-Tight Rotors ... 14 Switch off Centrifuge ... 14 System Menu... 15 Flowchart System Menu... 15 Maintenance and Care... 16 Cleaning Intervals ... 16 Basics ... 16 Cleaning... 16 Disinfection ... 17 Decontamination ... 18 Autoclaving ... 18 Maintenance and Repair Services ... 18 Shipping and Disposal... 19 Troubleshooting ... 20 Mechanical Emergency Door Release ... 20 Troubleshooting by Guide ... 21 When to contact Customer Service ... 22 Chemical Compatibility Chart ... 23
Preface Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions.
Set Up Conditions
The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is forbidden to copy or pass on this information without explicit approval.
•
Plug the centrifuge only into sockets which have been properly grounded.
•
Turn OFF the centrifuge at the main switch. The mains plug must be freely accessible at all times.
Failure to follow the instructions and safety information in this instruction manual may void of the sellers warranty.
Press the STOP key to shut down the centrifuge. Pull out the power supply plug or disconnect the power supply in an emergency.
Items Supplied
•
Items
Quantity
Centrifuge GT 1 / 1R
1
Power supply cable
1
Instruction manual
1
CD
1
Check
If any parts are missing, contact your nearest Fisher Scientific sales representative.
As safety zone maintain a clear radius of at least 30 cm around the centrifuge. Do not place any dangerous substances within this security zone.
•
Set up in a well-ventilated environment, on a horizontally leveled and rigid surface with adequate load-bearing capacity.
Preparation •
It is the obligation of the operator to make sure, that the proper protective clothing is used. Mind the “Laboratory Biosafety Manual” of the World Health Organization (WHO) and the regulations in your country.
•
Do not make any changes to the mechanical components of the rotor.
•
Do not touch the electronic components of the centrifuge nor alter any electronic or mechanical components.
•
Use only with rotors which have been properly installed. Follow the instructions on the Thermo Scientific™ Auto-Lock™ rotor exchange in section Rotor Installation.
•
The centrifuge is to be used for its intended use only. Improper use can cause damages, contamination, and injuries with fatal consequences.
Do not use rotors, buckets or accessories that show any signs of removed protective coating, corrosion or cracks. Contact customer service for further advice or inspections.
•
Use only with rotors which have been loaded properly.
The centrifuge should be operated by trained specialists only.
•
Never overload the rotor.
In order to ensure safe operation of the Fisherbrand GT 1 / 1R centrifuge, general safety regulations must be followed. Mind the regulations in your country.
•
Always balance the samples.
•
Use only rotors and accessories approved for this centrifuge. Exceptions to this rule are commercially available glass or plastic centrifuge labware, provided they have been designed to fit rotor or adapter cavities
Intended Use This centrifuge is designed to separate sample mixtures of different densities like chemicals, environmental samples and other non-human body samples.
Precautions Observe the safety instructions. Not following these instructions can cause damage.
Centrifuge GT 1 / 1R
Preface |
1
and are approved for the speed or the RCF value of the rotor.
•
Never start the centrifuge when the centrifuge door is open.
•
Make sure the rotor is locked properly into place before operating the centrifuge.
•
Do not move the centrifuge while it is running.
•
Do not lean on the centrifuge.
•
Implement measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it is running.
•
Do not place anything on top of the centrifuge during a run.
•
If used for foodstuffs machinery, for cosmetics or pharmaceutical products, only use closed or aerosoltight containers for centrifugation.
•
Never open the centrifuge door until the rotor has come to a complete stop and this has been confirmed in the display.
•
The emergency door release may be used in emergencies only to recover the samples from the centrifuge, e.g. during a power failure (refer to the section Mechanical Emergency Door Release).
Hazardous Substances •
Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts and vessel have to be cleaned thoroughly.
•
Do not centrifuge explosive or flammable materials or substances.
•
The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone environment.
•
Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety precautions.
If centrifuging any hazardous materials mind the “Laboratory Biosafety Manual” of the World Health Organization (WHO) and any local regulations. When centrifuging microbiological samples from the Risk Group II (according to the “Laboratory Biosafety Manual” of the World Health Organization (WHO)), aerosol-tight biological seals have to be used. Look on the internet page of the World Health Organization (www.who.int) for the “Laboratory Biosafety Manual”. For materials in a higher risk group, extra safety measures have to be taken. •
•
•
If toxins or pathogenic substances have contaminated the centrifuge or its parts, appropriate disinfection measures have to be taken (refer to the section Disinfection). Extreme care should be taken with highly corrosive substances which can cause damage and impair the mechanical stability of the rotor. These should only be centrifuged in fully sealed tubes. If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area immediately.
Operating •
2 |
Do not open the centrifuge, while it is running. In any case of severe mechanical failure, such as rotor or bucket crash, the centrifuge is not aerosol-tight. In case of rotor failure the centrifuge can be damaged. Leave the room. Inform customer service.
Maintenance The centrifuge housing is not to be opened by the operator.
Symbols used on the Centrifuge This symbol refers to general hazards. CAUTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. This symbol refers to information on hazards, described within the manual. This symbol demands to disconnect mains before transporting or servicing the centrifuge.
Never use the centrifuge if parts of its cover panels are damaged or missing.
Preface
Centrifuge GT 1 / 1R
This symbol demands to check, if the rotor is installed correct by lifting it slightly at the handle. Refer to the section Rotor Installation. This symbol refers to electrical hazards.
Technical Specifications Technical Data The technical data of the Fisherbrand GT 1 / 1R Centrifuge is listed in the following table.
Feature
GT 1
GT 1R
Use in interior spaces Environmental conditions
Altitudes of up to 2 000 m above sea level Max. relative humidity 80 % up to 31 °C; decreasing linearly up to 50 % relative humidity at 40 °C.
Environmental Conditions during Storage and Shipping
Temperature: -10 °C to +50 °C Temperature: -10 °C to +50 °C Humidity: 15 % to 85 %
Humidity: 15 % to 85 %
+2 °C to +35 °C
+2 °C to +35 °C
Ventilated
Refrigerated
230 V
230 V
1 060 Btu/h;
1 170 Btu/h;
Overvoltage Category
II
II
Pollution Degree
2
2
IP
20
20
Running Time
99 h 59 min 50 sec, hold
99 h 59 min 50 sec, hold
Maximum Speed nmax
16 000 rpm
17 850 rpm
Minimum Speed nmin
300 rpm
300 rpm
Maximum RCF Value at nmax
24 328 x g
30 279 x g
Noise Level at Maximum Speed1
< 58 dB (A) (measured with a TX-150 rotor); < 61 dB (A) < 56 dB (A) (measured with a TX-150 (measured with a MicroClick™ rotor) 24x2 rotor)
Maximum Kinetic Energy
8.12 kJ
Permissible Ambient Temperature during Operation
Heat Dissipation1
Temperature Setting Range
Centrifuge GT 1 / 1R
10.1 kJ -10 °C to +40 °C
Technical Specifications |
3
Feature
GT 1
GT 1R
Height (open door / closed door)
660 mm / 310 mm
700 mm / 320 mm
Width
370 mm
460 mm
Depth
490 mm
670 mm
35 kg
74 kg
Dimensions
Weight without rotor 1
Front Side Measurement, 1 m in front of the instrument at 1.6 m height
Directives, Standards and Guidelines Region
Directive
Standard
Europe
2006/42/EC Machinery
EN 61010-1, 2nd Edition
220-230 V
2014/35/EU Low Voltage
IEC 61010-2-020, 2nd Edition
50 / 60 Hz
(Protective Goals)
EN 61326-1
2014/30/EC Electromagnetic Compatibility (EMC)
EN ISO 14971
2011/65/EC RoHS
EN ISO 9001
Directive on the Restriction of the use of certain Hazardous Substances in electrical and electronic equipment
Mains Supply The following table contains an overview of the electrical connection data. This data is to be taken into consideration, when selecting the mains connection socket.
Unit
Mains Voltage
Frequency
Rated Current
Power Consumption
Equipment Fuse
Building Fuse
Fisherbrand GT 1 Centrifuge
230 V
50 / 60 Hz
2A
310 W
5 AT
16 AT
Fisherbrand GT 1R Centrifuge
230 V
50 / 60 Hz
4A
750 W
15 AT
16 AT
Refrigerant Order no.
centrifuge
Refrigerant
Quantity
Pressure
GWP
CO2e
75007234
Fisherbrand GT 1R Centrifuge
R-134a
0.315 kg
21 bar
1430
0.45 t
Refrigerant contains fluorinated greenhouse gases in a hermetically sealed system.
4 |
Technical Specifications
Centrifuge GT 1 / 1R
Rotor Selection Various rotors are available to choose from the following: 75005701
TX-150 swinging bucket rotor
75005702
TX-150 round buckets
75005703
TX-150 50mL conical buckets
75005704
TX100S clinical swinging bucket rotor with sealed carriers
75005705
TX100 clinical swinging bucket rotor with carriers
75005706
M10 microplate swinging bucket rotor
75005723
M10 buckets
75005721
M10 sealed buckets
75005600
MT12 microtube swinging bucket rotor
75005709
HIGHConic™ III fixed angle rotor
75003623
CLINIConic™ fixed angle rotor
75005715
MicroClick™ 24x2 microtube rotor
75005719
MicroClick™ 30x2 microtube rotor
75005720
8x8 PCR Strip rotor
75003694
8 x 50 mL Individually Sealed rotor (only usable in the Centrifuge GT 1R)
75005765
MicroClick™ 18x5 microtube rotor (only usable in the Centrifuge GT 1R)
75003602
Microliter 48x2 microtube rotor
For further details on rotor use and safety, additional accessories, adapters and spare parts, refer to the rotor manual. For more information visit our website at fishersci.com
Centrifuge GT 1 / 1R
Technical Specifications |
5
Transport and Set Up Before Setting Up
•
To prevent possible injuries, at least two people should lift and carry the centrifuge by holding it at the bottom from opposite sides.
1. Check the centrifuge and the packaging for any shipping damage. Inform Fisher Scientific Customer service immediately if any damage is discovered. 2. Remove the packaging. 3. Check, if the items supplied are complete. Refer to the section Items Supplied. If the items supplied are incomplete, please contact Fisher Scientific.
Location The centrifuge is only to be operated indoors. The set-up location must fulfill the following requirements: •
A safety zone of at least 30 cm must be maintained around the centrifuge. People and hazardous substances must be kept out of this safety zone while centrifuging.
•
The supporting structure must be stable and free of resonance.
•
The supporting structure must be suitable for horizontal setup of the centrifuge.
•
The centrifuge is not to be exposed to heat and strong sunlight. WARNING: UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.
•
The set-up location must be well-ventilated at all times.
Transporting
Figure 1. Lifting the centrifuge at both sides. •
Transport the centrifuge and accessories upright within the associated packaging, if possible.
Note: Store the original centrifuge packaging. Contact a shipping company or the customer service for the transport. Always remove the rotor before moving the centrifuge. If you do not remove the rotor you might damage the centrifuge drive or centrifuge spindle.
Leveling CAUTION: If the centrifuge isn’t leveled, imbalances can occur and the centrifuge can be damaged. Do not place anything under the centrifuge feet to level the centrifuge. The centrifuge must be placed on horizontal and level supporting structures or benching. Horizontal level must be checked when moving the centrifuge to a new location.
WARNING: Always lift the centrifuge from both sides. Never lift the centrifuge by its front panel, its back panel or at its door. Always remove the rotor before moving the centrifuge.
6 |
Transport and Set Up
Centrifuge GT 1 / 1R
Mains Connection
Storage
Note: Plug the centrifuge into grounded electrical sockets only.
WARNING: When removing the centrifuge and accessories from use if necessary clean, disinfect or decontaminate the entire system. In doubt contact the Fisher Scientific customer service.
1. Turn off the power supply switch.
•
Before storing the centrifuge and the accessories, it must be cleaned and if necessary disinfected and decontaminated. Centrifuge, rotors, buckets and accessories have to be thoroughly dried before storage.
•
Store the centrifuge in a clean, dust-free location.
•
Be sure to place the centrifuge on its feet.
•
Avoid direct sunlight.
Shipping WARNING: Before shipping the centrifuge and accessories you have to clean and if necessary disinfect or decontaminate the entire system. In doubt contact the Fisher Scientific customer service.
Figure 2. Power supply switch on the backside of the Centrifuge GT 1
Before shipping the centrifuge please bear the following in mind: •
The centrifuge must be clean and decontaminated.
•
The decontamination must be confirmed in a decontamination certificate. Contact customer service for more details.
Figure 3. Power supply switch on the right side of the Centrifuge GT 1R 2. Check whether the cable complies with the safety standards of your country. 3. Make sure that the voltage and frequency correspond to the figures on the rating plate. 4. Establish the connection to the power supply with the connecting cable.
Centrifuge GT 1 / 1R
Transport and Set Up |
7
Control Panel Control Panel The control panel contains the keys and displays of the centrifuge (only the power switch is located on the right side (Centrifuge GT 1R) or on the backside (Centrifuge GT 1) of the device). Program Keys
Status
Speed / RCF value
Acceleration / Deceleration Profiles
Running Time
Temperature
1
Pulse Key
SOFT ACC
2
SOFT DEC
3 SPEED
TIME
Modify the displayed value
Speed / RCF value
PULSE
OPEN
START
STOP
TEMP
XG RPM
Changing the display mode
Open key
Start key
Stop key
Prewarming or Precooling
Keys The keys allow user intervention for controlling the operating mode as follows:
Key
8 |
Display contents Start Key
Press the START key to start a centrifugation run or to accept the current settings
Stop Key
Press the STOP key to manually end the centrifugation run
Open Key
Press the OPEN key to activate the automatic door release (possible only when device is switched on and when the rotor is fully stopped). Refer to the section Mechanical Emergency Door Release
Pulse Key
Press the PULSE key to immediately start the centrifugation run and accelerate up to the maximal permissible end speed (depending on the used rotor). Releasing the key initiates a stopping process at the highest braking curves
Arrow Keys
Use these keys in order to modify the displayed value
Snowflake Key
Press the snow symbol key for prewarming or precooling the centrifuge
Toggle Key
Use the Change key to change the display mode (Speed / RCF value)
Control Panel
Centrifuge GT 1 / 1R
Operation Note: Only display pictures of the Centrifuge GT 1R are shown. The display of the Centrifuge GT 1 is the same, except missing the details for temperature.
Switching on the Centrifuge 1. Turn on the power switch on the back of the device. The device performs a self-check of its software. a.
When the centrifuge door is closed the display shows:
The speed and time displays read 0. The current temperature inside the rotor chamber is displayed. b. When the centrifuge door is open the display shows:
Keep hands and objects well clear of the underside and side of the centrifuge door when closing. Close the centrifuge door by pressing down on it lightly in the middle or on both sides of it. The centrifuge door mechanism will click and lock in place. Lids should not be slammed as excessive force may cause damage or disrupt samples. Note: The centrifuge door should audibly click into place.
Rotor Installation The approved rotors for the Centrifuge GT 1 / 1R are listed in section Rotor Selection. Use only the rotors from this list in the centrifuge.Permitted accessories are listed in the rotor manuals. CAUTION: Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge. The centrifuge is equipped with an Auto-Lock locking system. This system is used to automatically lock the rotor to the centrifuge spindle. The rotor does not have to be bolted on to the centrifuge spindle. Proceed as follows:
The speed and time displays show the preset values; the set temperature inside the rotor chamber is displayed.
1. Open the centrifuge door and if necessary remove any dust, foreign objects or residue from the chamber. Auto-Lock and O-Ring must be clean and undamaged.
Open the Centrifuge Door Press the OPEN
key.
Close the Centrifuge Door Auto-Lock WARNING: Do not reach into the gap between the centrifuge door and the housing.
O-Ring
Use the emergency release only for malfunctions and power failures (refer to the section Mechanical Emergency Door Release). Ensure that the centrifuge platform is clear from objects.
Figure 4. Auto-Lock Centrifuge GT 1 / 1R
Operation |
9
2. Place the rotor over the centrifuge spindle and let it slide slowly down the centrifuge spindle. If necessary a light push may be required to ensure connection. The rotor clicks automatically into place. CAUTION: Do not force the rotor onto the centrifuge spindle. If the rotor is very light, then it may be necessary to press it onto the centrifuge spindle with little pressure. 3. Check if the rotor is properly installed by lifting it slightly on the handle or from beneath the rotor. If the rotor can be pulled up, then it must be reconnected to the centrifuge spindle. WARNING: If the rotor cannot be properly locked in place after several attempts, then the Auto-Lock is defective and you are not permitted to operate the rotor. Check for any damage to the rotor and the spindle. Damaged rotors must not be used. Keep the centrifuge spindle area of the rotor clear of objects. If in doubt, call Fisher Scientific Customer Service. CAUTION: Check that the rotor is properly locked on the centrifuge spindle before each use by pulling it at its handle. 4. If available close the rotor with the rotor lid. 5. Check the according rotor manual for further details on requirements and methods of closure. CAUTION: Be sure to check all sealing before starting any aerosol-tight applications. See the information in the rotor instruction manual. 6. Close the centrifuge door.
the chosen settings. The last profile is saved, if you restart the centrifuge.
LED Light Settings
Description
OFF
Acceleration and Deceleration with max. Power = Standard
SOFT ACC
Acceleration = Soft
SOFT DEC
Deceleration = Soft
SOFT ACC and SOFT DEC
Acceleration and Deceleration = Soft
Pre-Selecting Speed / RCF RPM stands for Revolutions Per Minute. RCF stands for Relative Centrifugal Force and allows better transfer of protocols between centrifuges and rotors of differing size. Ensure that the rpm or RCF is correctly set. 1. Press the TOGGLE key below the SPEED display to cycle through the RPM / RCF selection. The LED light will indicate if “RPM” or “RCF” is selected. RPM / RCF can be viewed during a run by pressing the toggle button. 2. Enter the desired value by holding the arrow keys below SPEED in the corresponding direction, until the desired value shows. First RPM / RCF will change in steps of 10. Holding a key pressed will change the runtime then in steps of 100 and then in steps of 1000. Press the START key to accept or wait 4 seconds until the centrifuge automatically saves the chosen values. Moving to setting time or temperature also automatically stores the set value. Note: The minimum motor speed is 300 rpm. Any extremely low rcf settings will be automatically increased to the minimum rcf at 300 rpm.
Acceleration / Deceleration Rates
Explanation of RCF-Value
The Fisherbrand GT 1 / 1R Centrifuge offers you 2 profiles: standard and soft. The setting is displayed above the ACCELERATION / DECELERATION key .
The Relative Centrifugal Force (RCF) is given as a multiple of the force of gravity g. It is a unit less numerical value which is used to compare the separation or sedimentation capacity of various centrifuges, since it is independent of the type of device. Only the centrifuging radius and the speed are used for its calculation:
Press the ACCELERATION / DECELERATION key to cycle through and set the available profiles. The LEDs show
r = centrifuging radius in cm 10 |
Operation
Centrifuge GT 1 / 1R
2
n RCF = 11.18 ------------- r 1000
the desired temperature is displayed. Temperature will change in steps of single degrees celsius.
n = rotational speed in rpm The maximum RCF value is related to the maximum radius of the tube opening. Remember that this value is reduced depending on the tubes and adapters used. This can be accounted for in the calculation above if required.
Running Time Pre-Selection 1. Press the key. This allows to change the set time using the arrow keys until the desired time is displayed. First runtime will change in steps of 10 second. Holding a key pressed will change the runtime by steps of single minutes, followed by steps of 10 minutes, followed by steps of single hours and at least by steps of 10 hours. This will continue until the limit of 99 hours and 59 minutes is reached. Enter the desired runtime in hh:mm or mm:ss.
The temperature cannot be adapted until the rotor has been positively identified; the speed display will then show END. When the rotor is not recognized (centrifuge door closed and START key not yet pressed, speed display “0”), the centrifuge ensures that the sample cannot freeze regardless of the rotor being used.
Pre-warming or Pre-cooling the Centrifuge Ensure the rotor, buckets and accessories are correctly in place and securely attached in the chamber. For setting the pretemp value for the centrifuge proceed as follows: 1. Press the SNOWFLAKE key in order to open the temperature selection menu. The display shows the message “PreTemp”. 2. Enter the desired value by pressing the repeatedly, until the desired value shows.
2. Press the START key to accept or wait 4 seconds until the centrifuge automatically saves the chosen values. Moving to setting speed / RCF or temperature also automatically stores the set value.
3. Press the START key
key
.
Continuous Operation 1. Press either arrow keys
until HOLD is displayed.
2. Press the START key to accept or wait 4 seconds until the centrifuge automatically saves the chosen values. During continuous operation, the centrifuge will continue running until you stop it manually.
Preselecting the Temperature You can preselect temperatures between -10 °C and +40 °C. To set the temperature, proceed as follows:
4. The centrifuge motor will start and a specific speed defined by the rotor. This improves air circulation within the chamber, resulting in improved temperature control throughout the chamber and rotor. The rotor chamber is cooled down or heated up to the preset temperature. 5. When the set temperature is reached, the centrifuge will beep and continue to hold the temperature. 6. Press the STOP key to end the pre-warming or precooling.The display shows the current temperature inside the rotor chamber.
1. Press the TEMPERATURE arrow keys. This allows to change the set temperature using the arrow keys until
Centrifuge GT 1 / 1R
Operation |
11
Programs The Fisherbrand GT 1 Centrifuge is able to save up to 4 programs; the Fisherbrand GT 1R Centrifuge is able to save up to 99 programs. It is only possible to save a program if the centrifuge is in standstill. Loading or saving of programs is not possible if the centrifuge is spinning.
Saving a Program Modify the speed, time and temperature to the desired setting.
Rotors can run at high speeds. Each rotor is specifically designed to run at its maximum speed with a defined load. For further details refer to the rotor manual supplied with the rotor. The rotors are designed to work with substance mixtures with a density of up to 1.2 g/ml. Above this density or if total load is above the maximum weight the following steps should be taken: •
Reduce the fill level.
•
Reduce the speed.
Use the formula:
For Direct Access Programs 1, 2, 3: Press and hold the desired program key 1, 2 or 3 for 4 seconds.
nadm = admissible speed
For Programs 4-99:
nmax = maximum speed
1. Press the folder key for 4 seconds. Use the SPEED arrow
Once the rotor has been properly installed, the main switch turned on and the centrifuge door closed, you may start centrifuging.
key , to scroll through until the desired number is selected. 2. Press the START key
Use of Tubes and Consumables
to confirm.
3. The program can now be named using up to 12 alphanumeric characters. Use the SPEED arrow key to scroll through the characters. Use the TIME arrow keys to move left or right. 4. Press the START key program.
to confirm and save the
To abort at any point press the STOP key
.
Press one of the direct access program keys 1, 2, 3.
•
They are being used at or above there minimum fill volume
•
They are not being used above their design life (age or number of runs)
•
They are inspected for damage
Press the START key on the control panel. The centrifuge accelerates to the pre-set speed with the time display active.
For Programs 4-99 to scroll
Centrifugation Maximum Loading WARNING: Injuries with fatal consequences can occur when using substance mixtures with a higher density than 1.2 g/cm3 at maximum speed.
Operation
Rated to or above the selected rcf to be spun at
Starting Centrifuge Program
For Direct Access Programs 1, 2, 3
12 |
•
Refer to the manufacturers data sheets for further information.
Selecting a Program
Press the folder key. Use the SPEED arrow keys through until the desired program is selected.
Care should be taken to ensure that the tubes and bottles used in the centrifuge are:
If the speed setting is higher than the maximum permissible speed or RCF-value for the particular rotor, then after starting the display will show the message “Limit” – followed by the maximum RPM- or RCF-value of the inserted rotor once the centrifuge has been started. Within 10 seconds of the error message it is possible to accept the highest RPM / RCF of the inserted rotor by pressing START key . The centrifuge will then continue run for the set time and at the set temperature. If no action is taken within 10 seconds, the centrifuge will continue to decelerate. A new speed setting can be selected using the SPEED arrow keys and has to be confirmed by pressing the START key
.
Centrifuge GT 1 / 1R
If no action is taken, the centrifuge will decelerate until stop, then the lid should be opened and the rotor checked.
Imbalance Indicator The centrifuge is fitted with an imbalance detector, to ensure safety. If an imbalance is detected at speeds higher than approx. 300 rpm an error message “Imbalance load” will be displayed. Imbalance at high speed may indicate a tube breakage or leak or rotor crash. Therefore additional care should be taken depending on the samples loaded. The run will terminate. Once the run is complete, the rotor and load should be checked, ensuring that all buckets are greased and can swing free and that the tubes are balanced by following the rotor instruction manual. For information on troubleshooting, refer to the section Troubleshooting by Guide.
Stopping the Centrifugation Run
Short-term Centrifugation For quick centrifugation, the Centrifuge GT 1 / 1R has a PULSE-function. By holding down the PULSE key , spinning will start at maximum acceleration rate, continue at maximum rotor speed until the key is released. The rotor then decelerates at maximum rate. Preset values for acceleration, deceleration and speed or RCF are disregarded for the PULSE function. The centrifuge accelerates and brakes at maximum power. Any rpm or RCF entered beforehand is overridden. Note: The centrifuge accelerates to maximum speed, according to the rotor used. Check carefully whether you have to maintain a certain speed for your application. During the acceleration process, time is counted forwards in seconds. The reading stays displayed until the centrifuge door is opened.
Removing the Rotor To remove the rotor, proceed as follows:
With Pre-Set Running Time If the run time is preset, the centrifuge will run at the selected speed until the desired run time is reached. It will then automatically decelerate and stop. Once stopped “RUN COMPLETED” will be displayed and if selected the display will flash. Access to the chamber and rotor can be gained by pressing the OPEN key . If selected the door will open automatically.
1. Open the centrifuge door. 2. Grab the rotor handle and fully depress the Auto-Lock button. At the same time, pull the rotor vertically upwards and remove it from the centrifuge spindle. Make sure not to tilt the rotor while doing this.
You can also stop the centrifuging program manually at any time by pressing the STOP key .
Continuous Operation If you selected continuous operation (refer to the section Continuous Operation), you will have to stop the centrifuge manually. Press the STOP key on the control panel. The centrifuge will be decelerated at the set rate. The message “RUN COMPLETED” will be displayed. After pressing the OPEN key , the centrifuge door will open and you can remove the centrifuged samples.
Centrifuge GT 1 / 1R
Figure 5. Removing the rotor
Operation |
13
Aerosol-Tight Rotors For your protection when using an aerosol tight lid the rotor should only be removed with the lid closed. Note: Rotors supplied with a lid for aerosol-tight applications come with a mandrel, which belongs to the Auto-Lock. Be sure not to place the lid onto this mandrel to prevent it from being damaged.
Figure 6. Auto-Lock lid for aerosol-tight rotors CAUTION: Skin can be pierced by sharp mandrel tip. Do not touch the mandrel.
Switch off Centrifuge To turn off the centrifuge push the mains switch to “0”.
14 |
Operation
Centrifuge GT 1 / 1R
System Menu To get into the system menu, press and hold any key on the front panel turning on the centrifuge. Keep this key depressed until system menu appears in display. Navigate through the system menu using the TIME arrow keys .
Language
End of run beep?
Flowchart System Menu The system menu can be navigated using the SPEED arrow keys . The shown entry can be changed using the TIME and TEMP arrow keys . Press the START key to save this edit and quit the system menu. Press the STOP key to quit the system menu.
End of run Flash?
YES or NO
YES or NO
Keypad beep?
YES or NO
Beeper Volume
0-5
LCD powersave?
YES or NO
Door Auto open?
YES or NO
Program only mode
YES or NO
Values shown at some entries in the picture below are only examples. Note: A total number of cycles completed on the centrifuge is counted, since installation or a new main board was installed. Cycle counts should be periodically recorded to help with the identification of the total number of runs a rotor has completed.
English, Deutsch, Francaise Español, Italiano, Nederlands, Росский
Software ID: 0602 V01
NVRAM ID: 4753 V05
Cycle count total: 123456
Centrifuge GT 1 / 1R
System Menu |
15
Maintenance and Care Cleaning Intervals For the sake of personal, environmental, and material protection, it is your duty to clean and if necessary disinfect the centrifuge on a regular basis.
Maintenance
Recommended Interval
Rotor Chamber (Bowl)
Daily or when polluted
Rotor
Daily or when polluted
Accessories
Daily or when polluted
Filter Mat (Capacitor)
Every six months by a service technician.
Cabinet
Once per month
Ventilation Holes
Every six months
Rotor and Accessories Inspection After thoroughly cleaning rotors, they should be inspected for damage, wear and corrosion.
Metal Parts Ensure that the black protective coating is complete. It can be removed through wear and chemical attack and can lead to unseen corrosions. Any signs of corrosions, such as rust or white / metalic pitting, the rotor or accessories should be immediately removed from service. Particular attention should be taken with the bottom of buckets on swing out rotors and tube cavities on fixed angle rotors.
Plastic Parts
Basics CAUTION: Not rated procedures or agents could deteriorate the materials of the centrifuge and lead to malfunction. Refrain from using any other cleaning or decontamination procedure than those recommended here, if you are not entirely sure that the intended procedure is safe for the equipment.
Check for signs plastic crazing, fading, bruising or cracking. CAUTION: Do not run any rotor or accessories with sign of damage. Ensure that the rotor, buckets and accessories are within the service life and number of cycles. It is recommend that you have rotors and accessories inspected yearly as part of your routine service to ensure safety.
Use only approved cleansers. If in doubt, contact Fisher Scientific. •
Use warm water with a neutral detergent that is suitable for use with the materials. If in doubt contact the manufacturer of the cleaning agents.
•
Never use caustic cleaning agents such as soap suds, phosphoric acid, bleaching solutions or scrubbing powder.
•
Remove rotor and clean bowl with a small amount of cleaning agent, applied to a clean cloth.
•
Use a soft brush without metal bristles to remove stubborn residue. Afterwards rinse with a small amount of distilled water and remove any excess with absorbent towels.
•
Use only disinfectants with a pH of 6 to 8.
16 |
Maintenance and Care
Cleaning CAUTION: Before using any cleaning methods except those recommended by the manufacturer, users should check with the manufacturer of the cleaning agents that the proposed method will not damage the equipment. Clean as follows: 1. Clean rotor, buckets and accessories outside of the centrifuge bowl. 2. Separate all rotors, buckets, lids, adapters and tubes to allow thorough cleaning. 3. Rinse rotor and all accessories with warm water and a neutral detergent that is suitable for use with the
Centrifuge GT 1 / 1R