Guide
4 Pages
Preview
Page 1
Image Exposure Guide
EyeSuite IM900 LED Illumination v1.1 Diffuse Illumination Magnification
10 x or 16 x
Slit Illumination Level Background Level
Open @ 45 degrees, Diffused 7 2
Aperture
5
EyeSuite Exposure
1/90 Preset 1
Diffuse - Conjunctiva Magnification
10x or 16x
Slit Illumination Level Background Level
Open @45 degrees, Diffused, 4 1
Aperture
6
EyeSuite Exposure
1/125 Preset 1
Tips & Technique *Low magnification – overview images *Open slit fully – slit width can also be used to control exposure * Beware of unwanted reflection artefacts
Narrow Slit – Cornea Magnification
16x or 25x
Slit Illumination Level Background Level
< 0.2mm wide >60 degrees from mic 10 1
Aperture
1
EyeSuite Exposure
1/30 Preset 1
Image Exposure Guide
EyeSuite IM900 LED Illumination v1.1
Narrow Slit - Lens Magnification
16x or 25x
Slit Illumination Level Background Level
<0.2mm wide >60 degrees from mic 10 1
Aperture
1
EyeSuite Exposure
1/30 Preset 3 (25x); Preset 2 (16x)
Tips & Technique *Slit width must be less than 0.2mm to produce optical section *Maximise angle between illumination and microscope *consider the background *beware of specular reflection Wide Slit – Cornea Magnification 16x or 25x Slit Illumination 1-2mm wide, >60 degrees from mic Level 10 Background Level 1 Aperture
3
EyeSuite Exposure
1/90 Preset 2 (16x); Preset 3 (25x)
Magnification
16x or 25x
Slit Illumination Level Background Level
2-4mm wide, >60 degrees from mic 10 1
Aperture
4
EyeSuite Exposure
1/45 Preset 2
Wide Slit - Lens
Tips & Technique *Maximise angle of illumination * beware of specular reflections *reduce aperture to improve depth of field *experiment with position of background illumination
Image Exposure Guide
EyeSuite IM900 LED Illumination v1.1 Tangential – Cornea Magnification
16x or 25x
Slit Illumination Level Background
>4mm wide, >60 degrees from mic 10 Off
Aperture
3
EyeSuite Exposure
1/45 Preset 2
Tangential – Iris Magnification
16x or 25x
Slit Illumination Level Background
Wide Open, >60 degrees from mic 10 Off
Aperture
5
EyeSuite Exposure
1/125 Preset 1
Tips & Technique *Maximise angle to produce cross lighting *defocus slit can help with iris images * small aperture increases depth of field *
Sclerotic Scatter Magnification
16x or 25x
Slit Illumination Level Background
2 – 3mm defocused on limbus, 10 Off
Aperture
3
EyeSuite Exposure
1/20 Preset 1
Image Exposure Guide
EyeSuite IM900 LED Illumination v1.1 Indirect - Cornea Magnification Slit Illumination Level Background
16x or 25x 1-3mm wide, Decentred, 10 Off
Aperture
3
EyeSuite Exposure
1/45 Preset 2
Retro - Lens Magnification
16x or 25x
Slit Illumination Level Background
1-2mm wide, < 5 degrees, 10 Off
Aperture
3
EyeSuite Exposure
1/90 Preset 1
Fundus Magnification
10x or 16x
Slit Illumination Level Background
2-4mm wide 10 Off
Aperture
3
EyeSuite Exposure
1/90 Preset 1
These setting are provided as a guide only and changes to slit illumination level and exposure will be required in most cases to produce the optimum image All images on the RIGHT of the page were captured with BQ 900 and IM 900 using EyeCap. Images on the LEFT are for illustrative purposes only All images are copyright Haag-Streit AG and may only be used with their permission