UltraCW Automatic Cell Washing System Ver B Rev O Operation Manual June 2014

Document History Revision

Date

CO

Supersession

Revision Description

05 SEP 2013* 8686

M supersedes Revised layout for ease of navigation and locating information. F, G, H, I, J, K, L

N

07 FEB 2014* 9132

► Defined safety statements according to ANSI Z535.4 standards. N supersedes M ► Added caution statements as suggested by cell washer manufacturer (pages 12 and 34). ► Corrected Example 2 in section 10.2.

O

05 JUN 2014* 9553

► Corrected typographical error in section 10.2. O supersedes N ► Added instructions for installation of transport bolts, prior to moving/shipping the cell washer.

M

* Date submitted for Change Order review. Actual release date may vary.

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Contents Section I: General Information... 5 1 About this Manual... 5 1.1 1.2 1.3

Intended Audience... 5 Model References... 5 Copyright and Trademark... 5

2 Safety... 5 2.1 2.2 2.3

Safety Definitions... 5 Product Labels... 5 Avoiding Injury... 6

3 General Recommendations... 6 3.1 3.2

Intended Use... 6 General Use... 6

4 Specifications... 7 5 Compliance... 8 5.1 5.2

Regulatory Compliance... 8 WEEE Compliance... 8

6 Installation... 8 6.1

Location Requirements... 8 6.1.1 Placement... 8

7 Maintenance Schedule... 9

Section II: Configuration... 10 8 Cell Washer Installation... 10 8.1 8.2 8.3 8.4

8.5 8.6 8.7

Remove Packaging Materials... 10 Remove Transport Bolts... 10 Install Transport Bolts... 11 Connect the Saline and Drain Tubes... 11 8.4.1 Install Saline Supply Tubing... 11 8.4.2 Install Drain Tubing... 11 8.4.3 Connect the Cell Washer to the Saline Supply... 12 8.4.4 Connect the Cell Washer to the Drain... 12 8.4.5 Remove Saline Supply Tubing or Drain Tubing... 12 Initial Start Up... 12 Open and Close the Lid... 13 Release the Lid Lock... 14

9 Rotor Installation... 15 9.1 9.2 9.3 9.4 9.5

Install and Remove the Rotor... 15 Select the Rotor Type... 15 Balance the Rotor... 16 Install and Remove Tube Holder Inserts... 17 Load Tubes... 18

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10 Calibrate Saline Volume... 18 10.1 Dispense and Measure Saline Volume... 18 10.2 Determine Saline Volume Adjustment... 19 10.3 Change the Saline Volume... 20

11 Components... 21 11.1 11.2 11.3 11.4 11.5

Front, Lid, and Bowl... 21 Control Panel... 22 Rotor... 23 Side... 23 Rear... 24

Section III: Programming... 25 12 Processes and Process Steps... 25

12.1 Processes... 25 12.2 Process Steps... 26 12.2.1 Fill Step... 26 12.2.2 Spin Step... 26 12.2.3 Decant Step... 27 12.2.4 Drop Spin-Down Step... 27 12.2.5 Agitation Step... 27 12.2.6 Suspension Step... 28 12.2.7 Suspension-Agitation Step... 28

13 Programs and Parameters... 29

13.1 Display Mode... 29 13.2 Global Parameters... 29 13.2.1 View and Change Global Parameters... 31 13.3 Programs and Program Parameters... 31 13.3.1 Programs... 32 13.3.2 Program Parameters... 32 13.3.3 Select a Program... 33 13.3.4 View Parameter Summary for a Selected Program... 34 13.3.5 View all Program Parameters for a Selected Program... 34 13.3.6 Change Program Parameters... 35

Section IV: Operation... 36 14 Start and Stop Processes... 36 14.1 14.2 14.3 14.4

Start a Process... 36 Stop a Process... 37 Pause and Resume a Process... 37 Audible Alerts for Process Completion... 38

15 Sample Programs... 38 15.1 15.2 15.3 15.4 15.5

Sample Single-Cycle Washing Process... 39 Sample Multiple-Cycle Washing Process... 40 Sample Suspension-Only Process... 41 Sample Suspension with Agitation Process... 42 Sample Spinning-Only Process... 43

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15.6 Sample Spinning with Decanting Process... 44 15.7 Sample Spinning After Agitation Process... 45 15.8 Sample Agitation-Only Process... 46 15.8.1 Sample Agitation-Only Process (Started with the START WASH Button)... 46 15.8.2 Sample Agitation-Only Process (Started with the SPIN Button)... 47

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General Information

Section I: General Information 1

About this Manual

1.1

Intended Audience This manual is intended for use by end users of the UltraCW™ automatic cell washing system and authorized service technicians.

1.2

Model References Generic references are used throughout this manual to group models that contain similar features. For example, “UltraCW” refers to both the 115 V and 230 V models. If a feature or procedure applies to a specific voltage, it is stated as such.

1.3

Copyright and Trademark Helmer® and UltraCW are registered trademarks or trademarks of Helmer, Inc. in the United States of America. Copyright © 2014 Helmer, Inc. All other trademarks and registered trademarks are the property of their respective owners. Helmer, Inc., doing business as (DBA) Helmer Scientific and Helmer.

2

Safety The operator or technician performing maintenance or service on Helmer Scientific products must (a) inspect the product for abnormal wear and damage, (b) choose a repair procedure which will not endanger his/her safety, the safety of others, the product, or the safe operation of the product, and (c) fully inspect and test the product to ensure the maintenance or service has been performed properly.

2.1

Safety Definitions The following general safety alerts appear with all safety statements within this manual. Read and abide by the safety statement that accompanies the safety alert symbol.

2.2

WARNING

The safety statement that follows this safety alert symbol indicates a hazardous situation which, if not avoided, could result in serious injury.

CAUTION

The safety statement that follows this safety alert symbol indicates a hazardous situation which, if not avoided, could result in minor or moderate injury.

NOTICE

The safety statement that follows this safety alert symbol indicates a situation which, if not avoided, could result in damage to the product or stored inventory.

Product Labels The following general safety and information alerts appear on the product to identify potential hazards to the operator or service technician. Caution: Risk of damage to equipment or danger to operator

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Caution: Biohazard

5

General Information

2.3

Avoiding Injury ► Review safety instructions before installing, using, or maintaining the equipment. ► Do not move or bump the cell washer during operation. ► Before moving unit, disconnect the AC power cord and secure the cord. ► Never physically restrict any moving component. ► Avoid removing electrical service panels and access panels unless so instructed. ► Use supplied power cords only. ► Using the equipment in a manner not specified by Helmer may impair the protection provided by the equipment. ► Decontaminate parts prior to sending for service or repair. Contact Helmer or your distributor for decontamination instructions and a Return Authorization Number.

3

General Recommendations

3.1

Intended Use The Helmer UltraCW™ cell washing system is intended for use in blood banking and other laboratory procedures.

3.2

General Use Allow the cell washer to come to room temperature before switching power on.

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General Information

4

Specifications UltraCW™ Physical Height (1)

11.0” (280 mm)

Width

13.0” (331 mm)

Depth

15.5” (394 mm)

(2)

Weight

51.5 lbs (23.5 kg)

Maximum RPM

3500 ± 20

Kinetic Energy

250 Nm

Maximum Density

1.2 kg/dm³

Noise Level

≤ 44 dB(A)

(3)

Electrical Input Voltage and Frequency

110 V to 127 V, 60 Hz / 207 V to 253 V, 50-60 Hz

Fuses

2.5 A (115 V, 230 V)

Power Consumption Power Source

2.5 A (115 V), 1.5 A (230 V)

(4)

Varies (refer to product specification label)

(5)

Control and Monitoring Interface

Keypad with LCD display

Audible Alerts

Process complete, process stopped, error

Environmental

Application

► ► ► ►

Indoor use only Altitude (maximum): 2000 m Ambient temperature range: 5 °C to 40 °C Relative humidity (maximum for ambient temperature): 80% for temperatures up to 31 °C, decreasing linearly to 50% at 40 °C ► Transient overvoltages typically present on the MAINS supply ► Do not exceed maximum loaded weight (including tubes and contents). Maximum weight is printed on rotor.

(1) Height is 22.5” (572 mm) with lid open. (2) Add 1.25” (32 mm) to depth for saline and drain connectors. (3) Dependent on rotor. (4) Power consumption is measured in full-load Amperes. (5) Product specification label is located on the right side of the cell washer.

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General Information

5

Compliance

5.1

Regulatory Compliance Pollution degree: 2 (for use in USA and Canada only) This product is certified to applicable UL and CSA standards by a NRTL. Sound level (dependent on rotor): ≤ 44 dB(A)

5.2

WEEE Compliance The WEEE (waste electrical and electronic equipment) symbol (right) indicates compliance with European Union Directive WEEE 2002/96/EC and applicable provisions. The directive sets requirements for labeling and disposal of certain products in affected countries. When disposing of this product in countries affected by this directive: ► Do not dispose of this product as unsorted municipal waste. ► Collect this product separately. ► Use collection and return systems available locally. For more information on the return, recovery, or recycling of this product, contact your local distributor.

6

Installation

6.1

Location Requirements ► Has a sturdy, level surface ► Has a grounded outlet meeting national electric code (NEC) and local electrical requirements ► Minimum 13” (331 mm) above ► Minimum 5” (127 mm) on all sides ► Has access to a supply of saline solution ► Has access to a waste container or drain suitable to receive decanted saline and human blood waste product.

6.1.1

7

WARNING

Some saline solution contains a sodium azide preservative which may react with drain plumbing to form dangerous explosive azide salts. Check with the saline solution supplier before discharging waste solutions directly into normal drains.

NOTICE

For proper drainage, keep the waste container below the cell washer and the drain line as short as possible.

Placement WARNING

Unplug unit from AC power before moving.

NOTICE

Ensure rotor is empty before moving.

Maintenance Schedule Maintenance tasks should be completed according to the following schedule. Refer to the service manual for more detail on the various tasks.

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General Information

NOTE

These are recommended minimum requirements. Regulations for your organization or physical conditions at your organization may require maintenance items to be performed more frequently, or only by designated service personnel. Frequency Task

Daily

Inspect the tubing and drain and clear obstructions if necessary.



Inspect the tubing connections and secure them if necessary.



Clean and dry the interior after normal usage to prevent corrosion and contamination.



Weekly

Flush the system.



Clean the fill ports on the rotor.



Check the saline volume setting and calibrate it if necessary. Frequency varies by length of service.

 (1)

Monthly

SemiAnnually

Annually

 (2)

Check the rotor speed and calibrate it if necessary.



Inspect the rotor for wear, corrosion, and damage. Replace the rotor if these conditions exist.



Inspect the tube holders for wear and damage. Replace tube holders if they are worn or damaged, or after they have been in use for two years.



Clean the exterior.



Replace the supply and drain tubing.



Replace the pump tubing.



Replace the tube holder inserts for 10 mm x 75 mm tubes.



(1) During first month of usage. (2) After first month of usage. NOTE

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► Cell washers shipped before 04 October 2012: Replace the rotor every five years. ► Cell washers shipped 04 October 2012 and later: Replace the rotor every four years.

9

Configuration

Section II: Configuration 8

Cell Washer Installation

8.1

Remove Packaging Materials Before using the cell washer, open the lid and remove all packaging materials from the rotor and bowl. NOTE

8.2

Keep the packaging materials for future use.

Remove Transport Bolts The transport bolts are located on the bottom of the cell washer. Transport bolts prevent the motor from moving during shipping, and must be removed prior to use. The transport bolt removal tool is needed to perform this procedure. The tool is included but packaged separately from the cell washer. NOTICE

► Keep the transport bolts and removal tool for future use. ► Do not tip the cell washer on its back or side while removing the transport bolts.

Bolt removal procedure: 1 With the cell washer powered on, open the lid and remove the rotor. 2 Close the lid. Place the cell washer upside-down so the bottom faces upward. 3 On the bottom of the cell washer, locate the 2 transport bolts. 4 Use the bolt removal tool to remove the bolts. 5 Return the cell washer to the upright position. A B

Transport bolt removal tool (A). Transport bolts (B).

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Configuration

8.3

Install Transport Bolts Transport bolts prevent the motor from moving during shipping, and must be installed prior to moving or shipping the cell washer. The transport bolt removal tool is needed to perform this procedure. The tool is included but packaged separately from the cell washer. NOTICE

► The transport bolts must be installed prior to moving or shipping the cell washer. ► Do not tip the cell washer on its back or side while installing the transport bolts.

Bolt installation procedure: 1 With the cell washer powered on, open the lid and remove the rotor. 2 Close the lid. Place the cell washer upside-down so the bottom faces upward. 3 On the bottom of the cell washer, locate the threaded holes for the 2 transport bolts. 4 Use the bolt removal tool to install the bolts. 5 Return the cell washer to the upright position. NOTE

If the cell washer is being shipped to Helmer for service, pack the cell washer in the original packing materials. A B

Transport bolt removal tool (A). Transport bolts (B). 8.4

Connect the Saline and Drain Tubes Tubing is installed between the saline supply source and the cell washer, and between the cell washer and the drain. ► Small-diameter tubing is for the saline supply ► Larger-diameter tubing is for the drain The supply and drain tubing should be replaced according to the maintenance schedule. The tubing may need to be replaced more frequently depending on frequency of use and organizational requirements.

8.4.1

Install Saline Supply Tubing On the rear of the cell washer, firmly press the small-diameter tubing onto the saline supply hose barb. The tubing should cover the entire length of the hose barb. Twist the tubing while pressing it onto the hose barb for easier installation.

8.4.2

Install Drain Tubing On the rear of the cell washer, firmly press the large-diameter tubing onto the drain hose barb. The tubing should cover the entire length of the hose barb. Twist the tubing while pressing it onto the hose barb for easier installation.

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Configuration

8.4.3

Connect the Cell Washer to the Saline Supply If the fitting on the saline supply container is larger than the saline supply tubing, install an adapter between the saline supply container and the saline supply tubing. The adapter is included with the cell washer but is packaged separately. Connect the adapter: 1 Insert the small end of the adapter into the open end of the saline supply tubing. 2 Connect the large end of the adapter to the connector on the saline supply container.

8.4.4

Connect the Cell Washer to the Drain The cell washer has a gravity drain. Ensure the drain tubing flows downward, is as short as possible, and is not restricted.

8.4.5

WARNING

Some saline solutions contain a sodium azide preservative which may react with the drain plumbing to form dangerous explosive azide salts. Check with the saline solution supplier before discharging waste solution directly into sanitary drains.

NOTICE

► Keep the drain line clean and free of restrictions to avoid liquid accumulation in the bowl. Accumulated liquid may enter the tubes during the wash process, yielding unacceptable results. Excessive liquid in the bowl may also enter the motor compartment, causing motor failure. ► Ensure the entire length of drain tubing is lower than the bottom of the bowl so that liquid does not accumulate in the bowl.

Remove Saline Supply Tubing or Drain Tubing NOTICE

If the cell washer has been run, the saline supply tubing will be under a vacuum. If the vacuum is not released, the saline supply hose barb may be damaged when the saline supply tubing is removed.

Twist the tubing from side to side while simultaneously pulling the tubing off of the hose barb. 8.5

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Initial Start Up 1 Plug the power cord into a grounded outlet that meets the electrical requirements on the product specification label. 2 Switch the AC ON/OFF switch ON. ► The software version is displayed and all lamps are lit. ► After approximately 8 seconds, the control panel displays one of the following: ► If the lid was open when the power was turned on, a summary of the washing parameters for the selected program is displayed and no lamps are lit. ► If the lid was closed when the power was turned on, “OPEN LID” is displayed and the Lid Ready lamp is lit.

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Configuration

8.6

Open and Close the Lid The cell washer is equipped with an electronic lock that prevents the lid from being opened during operation. The Lid Ready lamp must be lit before the lid can be opened. NOTE

► If the lid is closed and there is no power to the cell washer, the lock will prevent the lid from being opened. ► If the lid must be opened when the cell washer is not powered on, or if the electronic lock is not working correctly, the lid can still be opened.

Left: Handle with latch in closed position. Right: Handle with latch in open position. Open the lid: CAUTION 1 2 3

Before opening the lid, ensure the rotor has stopped spinning.

On the control panel, confirm the Lid Ready lamp is lit. Lift the handle to release the latch. The Lid Ready lamp clears. Raise the lid.

Close the lid: CAUTION

Ensure that the lid handle is firmly latched in the downward position prior to operation.

NOTICE

To prevent damage to the cell washer or the contents, do not drop or slam the lid.

1 2

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With the handle in the open position, close the lid. Press down on the handle to close the latch. Parameters are displayed and the Lid Ready lamp lights.

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Configuration

8.7

Release the Lid Lock The cell washer equipped with an electronically-controlled lock that prevents the lid from being opened during operation. If the lock is not working properly, or if the lid is closed and there is no power to the cell washer, the lock may be released manually. The bypass tool is needed to perform this procedure. The bypass tool is included but packaged separately from the cell washer.

Left: Releasing the lid lock. Right: Bypass tool. Release the lid lock with the bypass tool: CAUTION 1 2 3 4

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Before releasing the lid lock, ensure the rotor has stopped spinning.

Switch the AC ON/OFF switch OFF. Disconnect the cell washer from AC power. Ensure the rotor has stopped spinning. On the front of the cell washer, insert the bypass tool into the access hole until it stops. While lifting the lid handle, gently press downward on the handle of the bypass tool until the lock releases.

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Configuration

9

Rotor Installation Either a 12-place rotor or a 24-place rotor may be installed in the cell washer. Both rotors can hold either 10 mm x 75 mm tubes or 12 mm x 75 mm tubes.

9.1

Install and Remove the Rotor The rotor must be installed for the cell washer to operate.

Left: Rotor shaft with alignment slot visible. Right: Grasping the rotor on the grip area. Rotor installation procedure: 1 Grasp the rotor on the grip area and place the rotor over the shaft. 2 Align the markings on the top of the rotor with the slots on the rotor shaft. 3 Lower the rotor onto the shaft. Rotor removal procedure: 1 Open the lid. 2 Grasp the rotor on the grip area and lift the rotor straight up. 9.2

Select the Rotor Type Either a 12-place rotor or a 24-place rotor may be installed in the cell washer. Both rotors can hold either 10 mm x 75 mm tubes or 12 mm x 75 mm tubes. The rotor type must be specified in the cell washer control system so the correct amount of saline solution is dispensed. The default factory setting is based on the type of rotor specified at the time of purchase. Specify the rotor: 1 Ensure the screen is in Display mode. If the screen displays a completion message, open the lid to clear the message and return to Display mode. 2 On the control panel, press and hold the parameter selection button () for about eight seconds. ► The VOLUME ADJUST XX parameter is displayed. 3 Press and release the parameter selection button () until the ROTOR: XX PLACE parameter is displayed. 4 Press and release the parameter value buttons ( or ) until the desired value is displayed. ► Select “12” for a 12-place rotor. ► Select “24” for a 24-place rotor. 5 Press the START WASH button to save the rotor configuration.

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Configuration

9.3

Balance the Rotor The rotor must be balanced for the cell washer to operate correctly. If the rotor is not balanced, the process is stopped, “IMBALANCE” is displayed and the Imbalance lamp illuminates. Tube holders and inserts must be installed correctly. If the empty rotor passes the balancing operation but the screen displays an imbalance message during normal operation, the tubes may be loaded unevenly or maintenance has not been performed correctly or at the specified interval. NOTE

Refer to the service manual for cleaning instructions.

Rotor balancing procedure: 1 Ensure all tubes have been removed from the tube holders. 2 Install the rotor and close the lid. 3 Change the Spin parameter values to perform the balance check. a Ensure the screen is in Display mode. If the screen displays a completion message, open the lid to clear the message and return to Display mode. b Press and release the parameter selection button () until each of the parameters in the following table are displayed. Parameter

Value

Spin(rpm)

3500

Tspin(min)

0

Tspin(sec)

20

d 4

For each parameter, press and release the parameter value buttons ( or ) until the values in the table above are displayed. Press the SPIN button to start the spin process. ► If “OPEN LID” is displayed and the Lid Ready lamp is illuminated, the rotor is balanced. ► If “IMBALANCE” is displayed and the Imbalance lamp is illuminated, the rotor is not balanced.

NOTE 5

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Refer to the service manual for additional instructions in balancing the rotor.

Reset the Spin parameters to their original values.

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Configuration

9.4

Install and Remove Tube Holder Inserts By default, the rotor is configured to use 10 mm x 75 mm tubes. If 12 mm x 75 mm tubes are used, the inserts must be removed from all tube holders on the rotor. The inserts secure the 10 mm tubes to prevent damage during operation. When the cell washer is shipped from the factory, inserts are installed. Use a small flat-head screwdriver to remove them. NOTE

► Tube holder inserts are available for purchase from Helmer. For replacement part numbers, refer to the service manual. ► Helmer recommends that empty tubes be installed in unused tube holders to avoid splashing saline solution inside the bowl.

Left: Slot in tube insert (circled). Right: Rotor with tube inserts (no insert, partially installed, fully installed). Tube holder insert installation procedure: 1 Open the lid. 2 Grasp the rotor on the grip area and lift the rotor straight up. 3 Place the rotor on a solid surface. 4 Align the key on the insert with the groove in the tube holder. 5 Press the insert into the tube holder all the way down, as shown above. Tube holder insert removal procedure: 1 Place the rotor on a solid surface. 2 While holding the tube holder with one hand, insert a flat-head screwdriver into the slot and slightly twist the screwdriver to raise the top of the insert. 3 Grasp the top of the insert and pull it straight out.

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Configuration

9.5

Load Tubes NOTICE

► Using 10 mm x 75 mm tubes without the tube holder inserts could result in tube damage or rotor imbalance. ► The acceptable tube height is 75 mm ± 1.5 mm. Tubes that are out of tolerance may not move freely around the fill port and may be damaged.

If loading fewer tubes than the rotor can hold, distribute the tubes evenly around the rotor to maintain balance. For example, if adding only three tubes to a 12-place rotor, position them at positions 1, 5, and 9.

12-place rotor with six tubes evenly distributed (positions 1, 3, 5, 7, 9, and 11).

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Calibrate Saline Volume

10.1

Dispense and Measure Saline Volume To calibrate the dispensed saline volume, the amount of dispensed saline must be measured and compared to the value that is displayed. The displayed value is the saline volume setting per tube multiplied by the number of places in the rotor. NOTE

Refer to chapter 7 (Maintenance Schedule) for the recommended calibration interval.

The following table shows the values that would be displayed if the SalWash/ml parameter were set to the recommended value: Rotor Type 12-place 24-place 12-place 24-place

Tube Size

Volume Setting (Per Tube)

10 mm x 75 mm 3.2 mL 12 mm x 75 mm 4.7 mL

Total Saline Volume Dispensed (±5%) 38.4 mL 76.8 mL 56.4 mL 112.8 mL

A volumetric measuring device (such as a graduated cylinder) of the appropriate capacity is necessary to accurately measure the amount of solution. Dispensing and measurement procedure: 1 Confirm the rotor type is set to the same as the rotor installed in the cell washer (12-place or 24-place). NOTE 2 3

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Refer to chapter 9 (Rotor Configuration) for rotor configuration instructions.

Select the program that contains the desired saline volume value. Open the lid.

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Configuration

4

5

On the control panel, press and hold the SALINE button for about four seconds. ► “CALIBRATE XXX.X ml” is displayed. ► The cell washer is now in Calibration mode. ► XXX.X represents the total saline volume that is dispensed. Hold the measuring device under the nozzle in the lid of the cell washer.

Use the volumetric measuring device to capture the dispensed saline solution. 6

7

On the control panel, press the CHECK button. The Saline lamp illuminates. The volume of saline solution that was displayed is dispensed from the nozzle into the volumetric measuring device. When the process is complete, the Saline lamp clears. Observe the saline volume in the volumetric measuring device. Use this value to adjust the volume.

NOTE 10.2

To stop the flow of saline solution, press the STOP button on the control panel.

Determine Saline Volume Adjustment After measuring the total saline volume, compare the displayed value to the measured value. If the measured value is within ±5% of the displayed value, no change is needed. If the measured value varies more than ±5% from the displayed value, the saline volume must be calibrated. The saline volume can only be adjusted in increments equivalent to 0.5% of the measured value. Adjustment determination procedure: 1 Compare the total volume displayed to the total volume measured. ► Example 1: A check is performed for a 12-place rotor with 10 mm x 75 mm tubes and a volume of 3.2 mL per tube. The displayed value is 38.4 mL, but the measured volume is 41.0 mL. ► Example 2: A check is performed for a 24-place rotor with 12 mm x 75 mm tubes and a volume of 4.7 mL per tube. The displayed value is 112.8 mL, but the measured volume is 106.8 mL. ► Example 3: A check is performed for a 12-place rotor with 10 mm x 75 mm tubes and a volume of 3.2 mL per tube. The displayed value is 38.4 mL, but the measured volume is 38.6 mL. 2 Determine if an adjustment is needed. If the measured volume is within ±5% of the displayed value, an adjustment is not needed. ► Example 1: ±5% of 38.4 = 1.92. 38.4 ± 1.92 = 36.5 to 40.4. Because 41.0 is above the tolerance, an adjustment is needed. ► Example 2: ±5% of 112.8 = 5.64. 112.8 ± 5.64 = 107.2 to 118.4. Because 106.8 is below the tolerance, an adjustment is needed. ► Example 3: ±5% of 38.4 = 1.92. 38.4 ± 1.92 = 36.5 to 40.4. Because 38.6 is within the tolerance, an adjustment is not needed.

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