UltraCW Operation Manual Ver B

Contents

Contents

i

About this manual... iv 1 Working safely... 1 1.1 1.2 1.3

General safety... 1 Electrical safety... 1 Chemical and biological safety... 2

2 Touring the cell washer... 3 2.1 2.2 2.3 2.4 2.5

Touring the front, lid, and bowl... 3 Touring the side... 4 Touring the rear... 4 Touring the control panel... 5 Touring the rotor... 6

3 Installing the cell washer...7 3.1 3.2 3.3

3.4 3.5 3.6 3.7

Recording identification information... 7 Selecting an appropriate location... 7 Powering the cell washer... 8 3.3.1 Installing the power cord... 8 3.3.2 Connecting to power... 8 3.3.3 Turning the power on and off... 8 Opening and closing the lid... 9 Removing packaging materials... 9 Removing the transport bolts... 10 Connecting to the saline supply and drain... 11 3.7.1 Installing and removing the supply and drain tubing... 11 3.7.2 Connecting to the saline supply... 12 3.7.3 Connecting to the drain... 12

4 Programming the cell washer... 13 4.1

4.2 4.3

4.4

Understanding processes and process steps... 13 4.1.1 Understanding processes... 13 4.1.2 Understanding process steps... 13 Understanding programs and parameters... 16 4.2.1 Understanding global parameters... 16 4.2.2 Understanding programs and program parameters... 18 Viewing and changing global parameters... 20 4.3.1 Returning to display mode... 20 4.3.2 Viewing global parameters... 20 4.3.3 Changing global parameters... 21 Working with programs and program parameters... 21 4.4.1 Selecting a program... 21 4.4.2 Viewing program parameters... 22 4.4.3 Changing program parameters... 23

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5 Using the cell washer... 24 5.1

5.2

5.3

5.4

Preparing the rotor... 24 5.1.1 Installing and removing the rotor... 24 5.1.2 Installing and removing the tube holder inserts... 25 5.1.3 Selecting the rotor type... 25 5.1.4 Confirming that the rotor is balanced... 26 5.1.5 Loading tubes... 26 Calibrating the saline volume... 27 5.2.1 Determining the saline volume to dispense into each tube... 27 5.2.2 Dispensing and measuring the total saline volume... 28 5.2.3 Determining how much to adjust the volume... 29 5.2.4 Adjusting the saline volume... 29 Starting and stopping processes... 30 5.3.1 Starting a process... 30 5.3.2 Pausing and resuming a wash process... 31 5.3.3 Stopping a process... 31 Controlling audible alerts for process completion... 31

6 Maintaining the cell washer... 32 6.1 6.2

6.3 6.4

Reviewing the preventive maintenance schedule... 32 Cleaning the cell washer... 33 6.2.1 Cleaning the exterior... 33 6.2.2 Cleaning the interior... 33 6.2.3 Flushing the system... 35 6.2.4 Cleaning the fill ports... 37 Replacing the pump tubing... 38 Replacing tube holders... 40

7 Understanding and addressing operational issues... 41 7.1 7.2 7.3 7.4

Understanding and addressing error messages... 41 Addressing performance problems... 44 Addressing equipment problems... 45 Releasing the lid lock... 46

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About this manual

iii

8 Reference information... 47 8.1

8.2

Technical Specifications... 47 8.1.1 Power... 47 8.1.2 Weight...47 8.1.3 Size... 47 8.1.4 Other specifications... 47 8.1.5 Operating conditions... 47 8.1.6 Energy conservation and regulatory compliance... 47 8.1.7 Supplies... 48 Sample programs... 49 8.2.1 Sample single-cycle washing process... 49 8.2.2 Sample multiple-cycle washing process... 50 8.2.3 Sample suspension-only process... 51 8.2.4 Sample suspension with agitation process... 52 8.2.5 Sample spinning-only process... 53 8.2.6 Sample spinning with decanting process... 54 8.2.7 Sample spinning after agitation process... 55 8.2.8 Sample agitation-only processes... 56

Warranty... 58

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UltraCW™ Automatic Cell Washing System Operation Manual

About this manual

Welcome to the UltraCW™ Automatic Cell Washing System Operation Manual. This section explains the symbols and conventions used in this manual, copyright information about this document, and trademark information for products supplied by Helmer.

Symbols and conventions Several symbols and conventions are used in this manual.

Warnings A Warning is used to call attention to a condition or possible situation that could cause injury to the operator. Warnings are identified as follows: Warning:

!

This is a sample of a warning: Follow all chemical handling and disposal requirements and procedures specified by your organization.

Cautions A Caution is used to call attention to a condition or possible situation that could damage or destroy the equipment or the operator’s work. Cautions are identified as follows:

!

Caution:

This is a sample of a caution: Be sure the tubing is free of obstructions. Blocked tubing can cause fluid to back up and cause motor failure.

Notes Notes contain additional information about a topic. Notes are used to provide information about how a topic relates to another topic, or background information about a design characteristic. Notes are identified as follows: NOTE:

This is a sample of a note: Tubing kits are available for purchase through Helmer.

Model references Generic references are used throughout this manual to group models that contain similar features. For example, “UltraCW” refers to both the 115 V and 230 V models. If a feature or procedure applies to a specific voltage, it is stated as such.

Copyright and trademark information Copyright © 2008 Helmer, Inc. UltraCW™ is a registered trademark of Helmer, Inc. in the United States of America. All other trademarks and registered trademarks are the property of their respective owners.

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Working safely

Working safely

This section describes general safety information for installing, using, and maintaining the UltraCW Automatic Cell Washing System (“cell washer”). Your organization may provide additional safety information.

1.1

General safety To avoid injury to yourself and the cell washer, follow these safety instructions: ► Use the cell washer for the purpose for which it was designed. Avoid using the cell washer to process materials that are flammable, explosive, highly corrosive, or materials that produce high levels of energy when they react with one another. ► Do not use the cell washer if its components are damaged. Notify the appropriate personnel in your organization for guidance regarding usage and maintenance. ► Never attempt to physically restrict any of the moving components. ► Do not move or bump the cell washer during operation. ► Before performing the procedures in this manual, review the specific safety instructions for them. ► Perform only the maintenance described in this manual. Maintenance other than that specified in this manual should only be performed by technical service representatives authorized by Helmer.

1.2

Electrical safety !

Warning:

The cell washer has the potential of being a shock hazard. Review all safety instructions.

Review the following safety instructions before installing, using, or maintaining the cell washer: ► Inspect all electrical equipment and address any problems prior to installation. ► Avoid removing covers from the cell washer unless instructed to do so. ► Use only the power cords supplied with the cell washer.

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2

UltraCW™ Automatic Cell Washing System Operation Manual

1.3

Chemical and biological safety Review the following safety instructions before installing, using, or maintaining the cell washer: ► Only use cleansers and disinfectants with a pH of 5 to 8. ► Do not use an autoclave to clean any components of the cell washer.

!

Warning:

In addition to the instructions included in this manual, follow all chemical handling and disposal requirements and procedures specified by your organization.

Chemical handling and disposal requirements for your organization may include the following: ► Use of appropriate protective equipment when cleaning the cell washer ► Use of specific cleansers and cleaning supplies ► Appropriate handling and disposal of materials that are infectious, toxic, pathological, radioactive, or otherwise biologically or environmentally harmful ► Notification of appropriate chemical and biological safety personnel when handling or disposing of materials that are infectious, toxic, pathological, radioactive, or otherwise biologically or environmentally harmful

!

Warning:

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Before sending parts to Helmer or your distributor for service or repair, decontaminate them as appropriate. Any items that have not been decontaminated appropriately will not be accepted. Documentation stating that the contents are not contaminated and are safe to handle must accompany all returns. Contact Helmer or your distributor for decontamination instructions and a return authorization number.

2

Touring the cell washer

Touring the cell washer

3

Congratulations on your purchase of an UltraCW Automatic Cell Washing System. The cell washer provides advanced capabilities and outstanding benefits to complete your cell washing tasks quickly, easily, and dependably. This section gives a brief overview of the components of the cell washer, as well as how to find identifying information. Note that some components vary by options selected at the time of purchase.

2.1

Touring the front, lid, and bowl A B

G H

C D

I J

E

F

K

Left: Front of cell washer with lid open and rotor installed. Right top: Bowl with rotor removed. Right bottom: Access hole Label A B C D E F G H I J K

Description Lid handle Latch Sight window Nozzle Rotor Control panel Drainage system Bowl Drain Rotor shaft Access hole

Function Releases the lid lock and opens the lid Latches the lid in the closed position Provides visual access to the cell washer during operation. Used for measuring rotor speed. Directs the saline wash solution into the rotor Holds the tubes Controls and displays the status of the cell washer Contains the liquid used during processing Contains the liquid used during processing Drains waste fluid from the bowl Spins the rotor Provides access to the lid lock

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2.2

UltraCW™ Automatic Cell Washing System Operation Manual

Touring the side A

B

C

D

View of right side of cell washer Label A B C D

2.3

Description Fuse Power switch Power connector Product Specification label

Function Prevents current overload Turns the cell washer on and off Interface for the power cord Provides the model number, serial number, and electrical requirements for the cell washer

Touring the rear A

B C

View of rear of cell washer Label A B C

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Description Access door Drain connector Supply connector

Function Provides access to the pump and pump tubing Drains saline solution from the cell washer Supplies saline solution to the cell washer

Touring the cell washer

2.4

5

Touring the control panel A

B

C

D E

F

G

H

I

J

K

L

M

N

O

Control panel (shown with cell washer turned off) Label A

Description Message screen

B

SPIN button

C

START WASH button

D

Stop lamp

E

STOP button

F G H I

Imbalance lamp Spin lamp Lid Ready lamp Parameter selection button Parameter value buttons

J, K

L M

Saline lamp SALINE button

N O

Check lamp CHECK button

Function Displays process information, programming prompts, and error messages In display mode: Starts the process that uses the spinning group of parameters of the selected program In display mode: Starts the process that uses the washing group of parameters of the selected program In programming mode: Saves any changes being made to values in the current parameter menu Indicates when the rotor is moving after a process has been paused or stopped In processing mode: Stops the current process In programming mode: Cancels any changes being made to values in the current parameter menu In calibration mode: Stops the flow of saline solution Indicates when the rotor is not balanced Indicates when the rotor is moving Indicates when the lid is ready to be opened In display mode: Enters programming mode In programming mode: Selects a parameter In programming mode: Increases or decreases the value for the selected parameter. Also toggles between the Shakings and ShakeT(min) global parameters In display mode: Selects a program Indicates when saline solution is being dispensed In display mode: Enters calibration mode In processing mode: Starts the Refill sequence of the cleaning program Indicates when a process has been paused In processing mode: Pauses the process if it was started using the START WASH button In calibration mode: Starts the flow of saline solution 360084-1/H

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2.5

UltraCW™ Automatic Cell Washing System Operation Manual

Touring the rotor D A

E

B

F G

C

H

Left: Top view of 12-place rotor with inserts installed. Right: Bottom view of 12-place rotor Label A

Description Tube holder insert

B C D

Optical reference Fill port Alignment markings

E F

Tube holder Rotor lock

G H

Clip Ring

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Function Holds 10 mm x 75 mm tubes in the tube holders. Removed when using 12 mm x 75 mm tubes Provides a reference for testing the rotor speed Directs saline solution into the tubes Provides a reference to align the rotor with the rotor shaft Holds the tube to be processed Holds the tube holder during the decant step of the wash process Secures the tube holder to the ring on the rotor Holds the tube holders on the rotor

Installing the cell washer

3

Installing the cell washer

7

After you record identifying information and select an appropriate location for the cell washer, install the cell washer.

3.1

Recording identification information For easy reference, write the serial number on the front of this manual. The serial number is needed to provide efficient service. The Product Specification label is located on the right side of the cell washer next to the power connector.

A

Type: UltraCW

B

Serial Number 0000000

C

110-127Vac

Volts

Freq

60 Hz

Kinetic Energy 250Nm

Amps 2.5

Power

Noblesville, IN USA www.helmerinc.com

200 Watts

Max. Density 1.2Kg/dm3

2006 RPM 3500R/min Made in Switzerland

D

Product Specification label (sample) Label A B C D

3.2

Description Type (Model) Serial number (S/N) Power requirements Date of manufacturing

Selecting an appropriate location To optimize cell washer operation, the location for your cell washer must meet the following requirements: ► A sturdy, level surface to provide support and proper drainage ► Has a minimum of 5 inches (12 cm) of space around all sides of the cell washer for ventilation ► Has a minimum of 13 inches (33 cm) of space above the cell washer for opening the lid ► Has access to a grounded outlet meeting the electrical requirements as listed on the Product Specification label ► Has access to a saline supply ► Has access to a waste container or drain suitable to receive decanted saline and human blood waste product. For proper drainage, keep the waste container below the cell washer and the drain line as short as possible. ► Meets any additional requirements specified for your organization

!

Warning:

Some saline solution contains a sodium azide preservative which may react with the drain plumbing to form dangerous explosive azide salts. Check with the saline solution supplier before discharging waste solutions directly into normal drains. 360084-1/H

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UltraCW™ Automatic Cell Washing System Operation Manual

3.3

Powering the cell washer

3.3.1

Installing the power cord The power cord is included but packaged separately from the cell washer.

To install the power cord

► On the right side of the cell washer, connect the power cord to the connector.

3.3.2

Connecting to power

!

Caution:

As with any electronic device, before connecting to power, make sure the cell washer is at room temperature.

To connect to power

► Plug the power cord into a grounded outlet that meets the electrical requirements that appear on the Product Specification label on the cell washer.

3.3.3

Turning the power on and off The power switch is located on the right side of the cell washer.

To turn the power on

► Press the switch to the On ( │ ) position. The software version appears on the message screen and all lamps are lit. After about eight seconds, the control panel changes to one of the following views: ► If the lid was open when the power was turned on, a summary of the washing parameters for the selected program appears on the message screen and no lamps are lit.

Control panel after initialization when the lid was open. ► If the lid was closed when the power was turned on, OPEN LID appears on the message screen and the Lid Ready lamp is lit.

Control panel after initialization when the lid was closed. 360084-1/H

Installing the cell washer

9

To turn the power off

► Press the switch to the Off (  ) position.

3.4

Opening and closing the lid The cell washer is equipped with an electronically controlled lock that prevents the lid from being opened during operation. The Lid Ready lamp must be lit for you to open the lid. NOTE:

If the lid is closed and there is no power to the cell washer, the lock prevents the lid from being opened. If you must open the lid in this circumstance, or if the lock is not working properly, see Section 7.4, “Releasing the lid lock” for instructions to open the lid.

Left: Handle with latch in closed position. Right: Handle with latch in open position.

To open the lid 1 2 3

On the control panel, confirm that the Lid Ready lamp is lit. Lift the handle to the open position to release the latch. The Lid Ready lamp clears. Raise the lid.

To close the lid

!

Caution: 1 2

3.5

To prevent damage to the cell washer and its contents, avoid dropping or slamming the lid.

With the handle in the open position, close the lid. Press down on the handle to move the latch to the closed position. OPEN LID appears on the message screen and the Lid Ready lamp lights.

Removing packaging materials Before using the cell washer, open the lid and remove all packaging materials from the rotor and bowl. NOTE:

Keep the packaging materials for future use.

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UltraCW™ Automatic Cell Washing System Operation Manual

3.6

Removing the transport bolts NOTE:

Keep the transport bolts and removal tool for future use.

The transport bolts are located on the bottom of the cell washer. They keep the motor from moving during transport, and must be removed prior to use. You will need the transport bolt removal tool to perform this procedure. The tool is included but packaged separately from the cell washer.

To remove the transport bolts

!

Caution: 1 2 3 4 5

To avoid damaging parts, do not tip the cell washer on its back or side while removing the transport bolts.

With the power to the cell washer on, open the lid and remove the rotor. For more information and instructions, see Section 5.1.1, “Installing and removing the rotor.” Close the lid and place the cell washer upside down so that the bottom faces upward. On the bottom of the cell washer, locate the two transport bolts. Use the bolt removal tool to remove the bolts. Return the cell washer to the upright position. A B

View of right side of cell washer. A: Transport bolt removal tool. B: Transport bolts.

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Installing the cell washer

3.7

Connecting to the saline supply and drain

11

After installing the supply and drain tubing, connect to the saline supply and drain.

3.7.1

Installing and removing the supply and drain tubing Tubing is used to connect to the saline supply and to the drain. The tubing is included but packaged separately from the cell washer. The smaller-diameter tubing is for the saline supply. The larger-diameter tubing is for the drain. The supply and drain tubing should be replaced on a regular basis to prevent unexpected failure. The tubing may need to be replaced more frequently depending on how frequently the cell washer is used and policies for your organization. You will need the Drain/Fill Tubing Assembly to replace the tubing. This kit is available for purchase from Helmer. For the recommended replacement schedule, see Section 6.1, “Reviewing the preventive maintenance schedule.” For replacement part numbers, see Section 8.1.7 “Supplies.”

To install the saline supply tubing

► On the rear of the cell washer, on the supply connector, firmly press the smaller-diameter tubing around the connector until the tubing covers the barb. Twisting the tubing while pressing it onto the connector facilitates installation.

To install the drain tubing

► On the rear of the cell washer, on the drain connector, firmly press the larger-diameter tubing around the connector until the tubing covers the barb. Twisting the tubing while pressing it onto the connector facilitates installation.

To remove supply or drain tubing

!

Caution:

If the vacuum is not released, the connector could break off, requiring part replacement.

► While twisting to release the vacuum, pull the tubing off the connector.

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UltraCW™ Automatic Cell Washing System Operation Manual

3.7.2

Connecting to the saline supply If the connector or tubing on your saline supply is larger than that for the cell washer, you can use an adapter. The adapter is included but packaged separately from the cell washer.

To connect the adapter 1 2

3.7.3

Connect the smaller end of the adapter to the end of the supply tubing. Connect the larger end of the adapter to the connector on the saline supply.

Connecting to the drain The cell washer has a gravity drain. Ensure that the drain tubing flows downward, is as short as possible, and is not restricted in any way.

!

Warning:

Some saline solution contains a sodium azide preservative which may react with the drain plumbing to form dangerous explosive azide salts. Check with the saline solution supplier before discharging waste solutions directly into normal drains.

!

Caution:

If the drain line becomes kinked or restricted, drained liquid can accumulate in the bowl and enter the tubes during the wash process, yielding unacceptable results. Excessive liquid in the bowl may also enter the motor compartment, causing motor failure.

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Programming the cell washer

Programming the cell washer

13

When you program the cell washer, you are defining a process which will later be automatically executed when you start the program. You control the steps of the process by setting parameters, which are viewed and changed from two menus: the Global menu and the Program menu. This section describes the major types of processes, the steps that make up those processes, and the parameters used to control the steps. Once you are familiar with the concepts, you will be ready to plan programs for your processes, then program the cell washer to execute them at the touch of a button. For sample programs, see Section 8.2, “Sample programs.”

4.1

Understanding processes and process steps The cell washer is capable of executing five major types of processes. Each process consists of one or more process steps.

4.1.1

Understanding processes The major types of processes are as follows: Washing processes. Washing processes include at least one basic wash cycle, which is composed of three steps: Fill, Spin, and Decant. Washing processes may also include one or more of the following steps: Agitation, Drop spin-down, Suspension, and Suspension-agitation. Suspension processes. Suspension processes include one Suspension step, but exclude Fill, Spin, and Decant steps. Suspension processes may also include a Suspension-agitation step after the Suspension step. Spinning processes. Spinning processes include one Spin step, but exclude a Fill step. Some Spinning processes may also include one or more of the following steps: Agitation and Decant. Agitation processes. Agitation processes include one Agitation step, but no other steps. Cleaning process. The cleaning process is a preset sequence that requires user intervention. After the user prepares the system for cleaning, the following steps are executed: Fill, Spin, Decant, Agitation, Decant. The user must then prepare the system for normal operation, then start a Refill sequence to complete the process.

4.1.2

Understanding process steps Each process consists of one or more process steps. Each process step is controlled by setting a combination of global and program parameters. For more information about global and program parameters, see Section 4.2, “Understanding programs and parameters.”

4.1.2.1

Understanding the Fill step During the Fill step, the tubes containing cells are filled with a specified amount of saline solution. As the rotor spins at 1100 r/min, saline is pumped from the reservoir through the flow meter to the saline dispensing nozzle. The nozzle directs the saline into the rotor, then the fill ports direct the saline into the tubes. Saline enters the tubes in a direct stream to maximize cell re-suspension. For washing processes, this step is always executed first in the basic wash cycle (Fill, Spin, Decant). You specify the amount of saline solution with the SalWash/ml program parameter. For sample processes that include this step, see Section 8.2.1, “Sample single-cycle washing process” and Section 8.2.2, “Sample multiple-cycle washing process.” 360084-1/H

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UltraCW™ Automatic Cell Washing System Operation Manual For the cleaning process, this step is always executed first. The amount is preset at 10 ml per place on the rotor. The fill volume exceeds the capacity of the tube to aid in flushing the drainage system.

4.1.2.2

Understanding the Spin step During the Spin step, the rotor accelerates to the specified speed, spins for the specified time, then stops quickly. This step separates out unwanted particles and creates a button of red blood cells at the bottom of each tube. The quick stopping action prevents the re-suspension of the red blood cells and dislodging of the cell button. For washing processes, this step is always executed second in the basic wash cycle (Fill, Spin, Decant). You specify the speed with the Wash(rpm) parameter, and the duration with the Twash(min) and Twash(sec) parameters. For sample processes that include this step, see Section 8.2.1, “Sample single-cycle washing process” and Section 8.2.2, “Sample multiple-cycle washing process.” For spinning processes, this step is always executed either first, or immediately after the optional Agitation step. You specify the speed with the Spin(rpm) parameter, and the duration with the Tspin(min) and Tspin(sec) parameters. For sample processes that include this step, see Section 8.2.5, “Sample spinningonly process,” Section 8.2.6, “Sample spinning with decanting process,” and Section 8.2.7, “Sample spinning after agitation process.” For the cleaning process, this step is always executed after the Fill step. The speed is preset at 1500 r/min, and the duration is preset at 10 seconds.

4.1.2.3

Understanding the Decant step During the Decant step, the rotor rapidly accelerates to the specified speed and decelerates, allowing the tubes to move back to the neutral position so the rotor lock can engage properly. The rotor spins in the opposite direction at the specified speed, while the rotor lock holds the tubes at a slight negative angle. Waste solution is expelled from the tubes by low centrifugal force, while virtually all the cells are retained in the tubes in the form of cell buttons. The waste solution is directed to the drain hole in the bowl by the drainage system. From there, the spent solution flows out of the cell washer into the drain tubing to the waste container or drain. For washing processes, this step is always executed third in the basic wash cycle (Fill, Spin, Decant). You specify the speed with the Decant(rpm) global parameter. For sample processes that include this step, see Section 8.2.1, “Sample single-cycle washing process” and Section 8.2.2, “Sample multiple-cycle washing process.” For spinning processes, this step is optional. For this step to be executed, both the SpinDecantM. global parameter and the SpinDecant program parameter must be enabled. This step is executed after the Spin step. You specify the speed with the Decant(rpm) global parameter. For a sample process that includes this step, see Section 8.2.6, “Sample spinning with decanting process.” For the cleaning process, this step is executed twice: once after the Spin step, then again after the Agitation step. The speed is preset at 600 r/min.

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Programming the cell washer

4.1.2.4

15

Understanding the Drop spin-down step During the Drop spin-down step, the rotor spins at 2000 r/min for the specified time. This action forces drops that are clinging to the sides of the tubes to the bottom of the tubes, thereby increasing the sample yield. This step is optional and available only for washing processes. For this step to be executed, both the DSpinDown(s) global parameter and the DSpinDown program parameter must be enabled. This step is executed after the Decant step. You specify the time with the DSpinDown(s) global parameter. For a sample process that includes this step, see Section 8.2.1, “Sample single-cycle washing process.”

4.1.2.5

Understanding the Agitation step During the Agitation step, the rotor accelerates to 200 r/min then stops, frequently and briefly, disrupting (breaking apart) the cell buttons. You must specify either the number of agitation cycles per agitation step with the Shakings global parameter, or the duration of each agitation step with the ShakeT(min) global parameter. For washing processes that consist of one basic wash cycle (Fill, Spin, Decant), this step is not executed. For washing processes that have multiple basic wash cycles, this step is executed between each basic wash cycle. You must specify either the number of agitation cycles per agitation step with the Shakings global parameter, or the duration of each agitation step with the ShakeT(min) global parameter. For a sample process that includes this step, see Section 8.2.2, “Sample multiple-cycle washing process.” For spinning processes, this step is optional. For this step to be executed, both the AgitSpinM. global parameter and the Agit.Spin program parameter must be enabled. This step is executed before the Spin step. You must specify either the number of agitation cycles per agitation step with the Shakings global parameter, or the duration of each agitation step with the ShakeT(min) global parameter. For a sample process that includes this step, see Section 8.2.7, “Sample spinning after agitation process.” For agitation processes, this step must be executed. You must set a series of global and program parameters to certain values depending on how you intend to start the process. For sample agitation-only processes, see Section 8.2.8, “Sample agitation-only processes.” For the cleaning process, this step is always executed between the Decant steps. The number of agitation cycles is preset at 5 cycles.

4.1.2.6

Understanding the Suspension step The Suspension step works the same as the Fill step, but is executed differently. For this step to be executed, both the SuspensionM. global parameter and the SalSusp/ml program parameter must be enabled. You specify the amount of saline that is dispensed with the SalSusp/ml program parameter. For washing processes, this step is optional. If enabled, this step is executed after the final Decant step, preceded by the optional Drop spin-down step. For a sample process that includes this step, see Section 8.2.1, “Sample single-cycle washing process.” For suspension processes, this step must be executed. This step is either the first or the only step. For sample processes that include this step, see Section 8.2.3, “Sample suspension-only process” and Section 8.2.4, “Sample suspension with agitation process.”

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