Instructions for Use
65 Pages
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Instructions for use
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Biofuge
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Safety regulations Instrument description Rotor program and accessories Transportation and hook-up Use of the centrifuge Maintenance and care Troubleshooting Technical data Index
Overleaf you will find a graphic representation of the control panel of the Biofuge fresco with a survey of the most important functions
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Use this manual to get acquainted with your centrifuge and its accessories. The manual helps you to avoid inappropriate handling. Make sure to keep it always close to the centrifuge. A manual that is not kept handy cannot provide protection against improper handling and thus against damage to persons and objects. This manual comprises chapters on
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How to use this manual
temperature
rotor turns
quick run
open lid stop start
“set“ keys
mains switch
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run time
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speed
Speed Resting: Run: End: Stop/run: Time Resting/end: During run: Temperature Resting/end/ run:
preset speed current speed; rotating light: rotor turns "End" error codes preset run time remaining run time or (with quick start) run time passed
current temperature of sample
Keys Start: Quick run:
normal start short-term acceleration as long as key is pressed, with indication of run time passed
Stop: "Set" keys:
open lid (possible only with mains switch ON) manual stop stepwise increase/decrease of preset values, accelerated change when pressed permanently
Short pressing of any of the "set" keys: switch from current to preset value
Error codes (troubleshooting see chapter "Troubleshooting") E-00: motor blockage (transport protection removed?) E-4: error in temperature measurement E-7: actual temperature out of range E-8: excess voltage E-10: internal error (call Service) E-11: internal error (call Service) E-23: deviation in internal temperature calibration br: power turned off during run or power failure Lid: lid turned loose or opened during run OPEN: with lid closed: safety circuit triggered Warnings can span several display panels
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Display
Open lid:
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The control panel of the Biofuge fresco
Contents
Proper use... 3 Improper use ... 3 Centrifuging hazardous materials ... 3 Handling ... 4 Conformity to current standards... 5 Safety instructions in this manual ... 5
The Biofuge fresco ... 7 Safety systems... 7 Features ... 8 Temperature regulation of the Biofuge fresco ... 9 "Quick run" operation ... 9 Pieces delivered... 10
Accessories ... 11 Rotor program ... 12 Adapters for rotor order no. 7500 3328 ... 13
Before use... 15 Where to install the centrifuge ... 15 Mains connection ... 15 Removing the transport protection... 15
Transport and installation... 17 Mains connection ... 17 Opening the lid ... 18 Emergency lid release... 18 Inserting the rotor ... 20 Permissible rotor temperature... 21 Lifetime of the rotor ... 21 Removing the rotor... 22 Loading the rotor ... 22 Maximum loading ... 22 Filling the centrifuge tubes ... 23 Aerosol-tight application... 24 Checking for aerosol tightness... 25 Placing the tubes in the rotor ... 26 Selecting the speed... 27 Selecting the run time ... 27 Preselected run time ... 27 Continuous operation ... 28 Setting the temperature... 28 Bringing the rotor to the desired temperature in the centrifuge ... 28 Starting the centrifuge ... 29 Changing the settings during the run ... 29 Stopping the centrifuge ... 30 Stopping with preset time ... 30 Stopping with continuous operation ... 30
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For your safety... 3
Operation ... 17
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Contents
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Contents
Maintenance and care ... 33 Maintenance to be performed by the customer . 33 Cleaning... 33 Disinfection ... 34 Decontamination... 36 Autoclaving ... 37 The Service of KENDRO... 38 Warranty conditions ... 38
Technical data... 45 Component parts and performance ... 45 The "Easycontrol" user interface... 47 Electrical connections/fuses... 49
Index ... 51 Autoclaving protocol... 57 Speed / RCF diagrams... 58
Troubleshooting ... 39
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Problems you can handle yourself... 39 In case you must call the Service... 43
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Short-time centrifugation ... 30 RCF value ... 31
For your safety
For your safety
Proper use
Heraeus centrifuges are manufactured according to current technical standards and regulations. Nonetheless, centrifuges may pose dangers if
The centrifuge is designed to separate liquidsuspended materials having different densities and particle size, respectively. The maximum sample density is 1.2 g/cm3 at maximum speed.
• their design is improperly changed • the safety instructions are not heeded Therefore anybody concerned with operation and maintenance of the centrifuge must read and follow the safety instructions. In addition, the pertinent regulations for prevention of accidents must be strictly followed. This manual is an integral part of the centrifuge assembly and must be kept close at hand at all times.
Improper use During a run, a safety zone of 30 cm around the centrifuge must be maintained where neither persons nor hazardous materials may be stationed. The centrifuge may cause harm to you or other persons and may damage material goods if you do not respect the following safety measures:
Centrifuging hazardous materials
• The centrifuge is neither made inert, nor is it explosion-proof. Therefore never use the centrifuge in an explosion-prone environment. • Explosive or flammable substances must not be centrifuged. The same holds for substances prone to react briskly with each other.
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• they are operated by untrained personnel
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• they are not used as designed
For your safety
• Strongly corrosive substances that may cause damage to materials and impair the mechanical strength of the rotor may be centrifuged only inside protective vessels.
Handling • Never use the centrifuge unless the rotor is properly mounted. • Never manually open the lid if the rotor still turns. • Use only original parts for the centrifuge. The only exception are common glass or plastic centrifuge tubes if these are approved for the rotor speed and RCF values of your rotor, respectively. • Never use the centrifuge with the lid open. • Never use the centrifuge if the paneling has been partially or totally removed. 4
• You may use the centrifuge only with a properly loaded rotor. You must not overload the rotor. • If the rotor or the lid shows visible traces of corrosion or wear, you must stop using it. • Strictly follow the rules and regulations for cleaning and disinfection.
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• Should toxins or pathogenic substances enter the centrifuge or its parts, you must carry out the proper procedures for disinfection (see "Maintenance and care – Disinfection").
• Changes in mechanical or electrical components may be carried out only by persons authorized to this effect by KENDRO Laboratory Products.
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• Do not centrifuge toxic or radioactive substances or pathogenic microorganisms unless you have taken proper precautions. Such precautions can e.g. consist of biological seals.
For your safety
Conformity to current standards Heraeus centrifuges are manufactured and tested according to the following standards and regulations:
Safety instructions in this manual This symbol denotes potential hazards to persons. This symbol denotes potential damage to the centrifuge or parts in its immediate surroundings.
• IEC 1010-1 / EN 61010-1
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for all voltages: General hints are marked with this symbol.
• IEC 1010-2 / EN 61010-2-020 − Pollution degree 2 In addition, you are asked to adhere to the pertinent regulations, in Germany
• CAN/CSA-C22.2 No. 1010.1-92
• Regulations for prevention of accidents BGV A2
• CAN/CSA-C22.2 No. 1010.2.020-94
• Regulations for prevention of accidents VBG 5
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− Overvoltage category II for 110 V only:
• Regulations for prevention of accidents VBG 7z • Regulations for prevention of accidents BGV D4
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For your safety
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for your notes
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The Biofuge fresco
The Biofuge fresco is equipped with a number of safety systems. Rotor chamber The rotor chamber consists of a stainless steel case which is sealed against the motor with a rubber cover. When the lid is closed, the rotor chamber is sealed against the surroundings by a rubber ring with a special profile. The rotor chamber is wrapped in evaporating tubing filled with the ecologically harmless cooling agent R134a, which is free from fluorinated/chlorinated hydrocarbons. Warning if lid is manually opened during a run, or if drive is overheated If the lid is manually opened during a run, or if the temperature of the drive exceeds a critical value, a corresponding message appears in the display ("Lid" and "OPEN", respectively). Lid lock You can open the lid only when the power is turned on and the rotor has practically come to a halt (< 80 rpm). You can start the centrifuge only if the lid is properly closed. 7
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The figure below shows the Biofuge fresco with the lid opened. In this state the standard display is speed x 1,000 and "OPEN".
Safety systems
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The Biofuge fresco
Features The Biofuge fresco is a refrigerated benchtop centrifuge for the preparation of sensitive samples in the biochemical and medical laboratory. The powerful refrigeration permits, at a room temperature of 25 °C, to maintain a sample temperature of 0 °C over prolonged periods of time even at the maximum speed of 13,000 rpm. The preset speed is reached in seconds. You can also spin samples for only a few seconds using the "quick run" key ( ) if this is required for the task in question. The extremely long-lived, maintenance-free induction motor provides quiet and vibration-free operation even at high speeds. The user-friendly "Easycontrol" control panel permits easy operation. With the centrifuge turned on and the lid closed, the preset speed and run time and the actual temperature are displayed before the run. During operation, the control panel shows the actual values; or upon briefly pressing any one of the "set" keys
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the preset values for speed, run time and temperature are indicated instead. After the run, the speed control panel displays "End". keys repeatedly, you increase or If you press the the corresponding preset value stepwise. If you press and hold down the chosen key, the respective value increases continuously, at first slowly and, after a few seconds, at an accelerated pace.
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Emergency lid release In order to permit you to remove samples even after a power failure, the centrifuge is equipped with an emergency lid release.
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The Biofuge fresco
The Biofuge fresco
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run time
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temperature of the environment
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location of the centrifuge
• rotor speed The Biofuge fresco is equipped with a powerful compression-type refrigeration. Possible settings are from -9 °C to +40 °C. For short-term operation requiring precise temperature control, both the rotor and the rotor chamber must be preadjusted to the desired temperature.
) is pressed, the As long as the "quick run" key ( rotor is accelerated with maximum power, potentially up to the maximum speed.
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During a run, the spinning rotor creates frictional heat. This leads to a temperature increase of the rotor, the tubes and finally of the samples. The extent of warming depends on:
"Quick run" operation
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Temperature regulation of the Biofuge fresco
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The Biofuge fresco
Pieces delivered The Biofuge fresco is delivered complete with: • a special cap nut for fixing the rotor • 10-mm tubular socket wrench for fastening the cap nut • cable for mains connection
tubular socket wrench order no. 2036 0072
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• this Manual
cap nut order no. 70056208
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• fixed-angle rotor 24 x 1,5 / 2 ml 7500 3328
Accessories
Accessories
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The Biofuge fresco is delivered complete with a fixedangle rotor with 24 holes for placing microliter tubes with a volume of 1.5 or 2.0 ml. In addition you may order three sets of adapters containing 24 reduction sleeves each. With these adapters you can centrifuge all commercially available microliter tubes with a volume between 0.2 and 0.6 ml as well as 0.2-ml PCR reaction vessels.
Please consult our sales documentation for a complete collection of accessories including technical data, order numbers and special low-cost package offers.
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A further option is a PCR-Strip rotor.
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Accessories
Rotor designation order no. places / volume
PCR-rotor
7500 3328
7500 3327
24 x 1,5 / 2 ml
4 x PCR-Strip
24 x 4
4x4
-1
13 000
13 000
-1
minimum speed nmin [ min ]
2 000
2 000
maximum RCF value at nmax
16 060
12 846
( 11 524* )
maximum radius [ cm ]
8,5
6,8
( 6,1* )
minimum radius [ cm ]
5,9
6,1
( 4,7* )
angle
40
45
acceleration / deceleration time [ s ]
15 / 16
15 / 16
min. temperature at nmax [ °C ]
0
0
aerosol-tight
yes (reduced filling)
yes (reduced filling)
permissible temperature range autoclavable (number of cycles)
-4 °C to +40 °C 121°C, (10 cycles)
-4 °C to +40 °C 121°C, (10 cycles)
maximum speed nmax [ min ]
[°]
relative to room temperature 25°C
* The values relate to vessel places 4 and 5 in the PCR-Rotor 12
( 32 x 0,5 )
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maximum permissible load [ g ]
Microliter rotor 24 x 2 ml PP
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Rotor program
Accessories
Dimensions (∅ x H)
Capacity
Number per Set
Color
Order No.
reduction sleeve PCR
6,2 x 20 mm
0,2 ml
24
gray
7600 3750
reduction sleeve
8 x 43,5 mm
0,5/0,6 ml
24
turquoise
7600 3758
reduction sleeve
6 x 46 mm
0,25/0,4 ml
24
red
7600 3759
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Adapter
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Adapters for rotor order no. 7500 3328
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for your notes
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Before use
The centrifuge may only be used indoors. Its location must meet the following criteria: • A safety zone of 30 cm around the centrifuge must be maintained. Hazardous materials must not be kept within this zone during centrifugation. • The substructure must be stable and resonancefree. A good support is provided by a plane laboratory bench or a large laboratory carriage with casters that may be locked. • To ensure sufficient air circulation, a minimum distance from the wall of 10 cm at the back and of 15 cm on each side must be kept. • The centrifuge must be protected from heat and direct sunshine. • The location should be well ventilated.
Make sure that the mains supply you use for the centrifuge meets the specifications printed on the type plate. Turn the mains switch off (press "0"); only then connect the centrifuge with the mains supply using the power cord supplied with the instrument.
Removing the transport protection Turn the instrument on. The display panel shows for about 6 s the routine internal software check sequence. Open the lid by pressing the "open lid" key and remove the transport protection for the rotor. Check that the rotor moves freely by lightly turning it, and make sure the rotor is tightly screwed on.
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Where to install the centrifuge
Mains connection
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Before use
Before use
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for your notes
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