Heraeus Fresco 17-21 Instruction Manual Aug 2012

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©2013 Thermo Fisher Scientific Inc. All rights reserved. HERAEUS is a registered trademark of Heraeus Holding GmbH licensed to Thermo Fisher Scientific. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd. Hypaque is a registered trademark of Amersham Health As. RULON A and Tygon are registered trademarks of Saint-Gobain Performance Plastics. Alconox is a registered trademark of Alconox. Ficoll is a registered trademark of GE Healthcare. Haemo-Sol is a registered trademark of Haemo-Sol. Triton X-100 is a registered trademark of Sigma-Aldrich Co. LLC. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Manufacturer: Thermo Fisher Scientific Robert-Bosch-Straße 1 D - 63505 Langenselbold Germany

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc. The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser. Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document, even if the information in the document is followed properly. This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents

ThermoFisher SCIENTIFIC

KON FORM ITATSE RKLARU NG

DECLARATION OF CONFORMITY

(€

Name und Anschrift des Herstellers und des Bevollmächtigten für die Zusammenstellung der relevanten technischen Unterlagen: Name and address of the manufacturer and of the authorized representative to compile the relevant technical documentation:

Thermo Electron LED GmbH Zweigniederlassu n g Osterode Am Kalkberg 37520 Osterode Germany Hiermit erklären wir, dass die nachstehend beschriebene Maschine Herewith we declare, that the machinery described below Besch rei bunq /descri otion

Marke / brand

Modellbezeichnunq / model name Modell Nr. / model no. Gültig ab Equipmentnr. Valid from equipment no.

: Labor-Zentrifuqe / centrifuqe : Heraeus : FRESCO 17, FRESCO 21 : 75002420 75002423 75002425 : 41385539

mit allen einschlägigen Bestimmungen der Richtlinie über ln-vitro-Diagnostika 98/79/EG in Übereinstimmung ist. is in conformity with all relevant terms of directive for in vitro diagnostic medical devices 98n9/EC. Die Maschine ist auch in Übereinstimmung mit allen einschlägigen Bestimmungen der Richtlinie 2004n 0 8/EG ü be r e I e ktrom ag n eti sc h e V e rt räg I i c h ke i t. The machinery is in accordance with all relevant terms of directives for electromagnetic compatibility 2004/108/EC.

Die Schutzziele nach Anhang 1 der Maschinenrichtlinie 2006/42/EG und der Niederspannungsrichtlinie 2006/9 s/EG we rden ei nge h alte n. The protection goals of Annex 1 for the directives machinery 2006/42/EC and low voltage 2006/95/EC are met.

Angewandte harmonisierte Normen/ Harmonized standards used: EN 61010-1:2004 EN 61010-2-020: 2006 EN 61010-2-101:2002 EN 61326-1: 2006 EN 61326-2-6: 2006 EN 5501 18:2007 Osterode. den O9.O7 .2012

,'+r--d.aqrz

id

Dr. Andreas Karl, Director R&D

Ort, Datum, Name, Funktion und Unterschrift / Place. date. name, function and

WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96. It is marked by the following symbol:

Thermo Fisher Scientific has entered into agreements with recycling and disposal companies in all EU Member States for the recycling and disposal of this device.

T Table of Contents Preface ... iii Scope of Supply ... iii Intended Use ...iv Accident Prevention ...iv Precautions ...v

Thermo Scientific

Chapter 1

Introduction and Description ... 1-1 Characteristics of the Heraeus Fresco 17 / 21 ... 1-2 Technical Data ... 1-3 Directives, Standards and Guidelines ... 1-4 Functions and Features ... 1-5 Mains Supply ... 1-5 Rotor Selection ... 1-6

Chapter 2

Before Use ... 2-1 Before Setting up ... 2-2 Transporting the Centrifuge ... 2-2 Location ... 2-2 Aligning the Centrifuge ... 2-3 Mains Connection ... 2-3 Storage ... 2-4

Chapter 3

Control Panel ... 3-1 Control Panel ... 3-2 Keys ... 3-2

Chapter 4

Operation ... 4-1 Switch on Centrifuge ... 4-2 Door Opening ... 4-2 Close Door ... 4-2 Rotor Installation ... 4-2 Entering Parameters ... 4-4 Centrifugation ... 4-8 Short-term Centrifugation ... 4-8 Removing the Rotor ... 4-9 Aerosol-tight Rotors ... 4-9 Audible Alarm ... 4-9 Turning off the Centrifuge ... 4-10

Chapter 5

Maintenance and Care ... 5-1 Cleaning Intervals ... 5-2 Cleaning ... 5-2 Disinfection ... 5-4

Heraeus Fresco 17 / 21

i

Contents

Decontamination ... 5-5 Autoclaving ... 5-5 Service of Thermo Fisher Scientific ... 5-6 Shipping and Deposing of Accessories ... 5-6 Chapter 6

Troubleshooting ... 6-1 Mechanical Emergency Door Release ... 6-2 Troubleshooting by User ... 6-3 When to contact Service Technician ... 6-5

Chemical Compatibility Chart ...A-1 Contact Information ...B-1

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P

Preface Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions. The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is forbidden to copy or pass on this information without explicit approval. Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty.

Scope of Supply Article number

Quantity Centrifuge Heraeus Fresco 17 / 21

1

Power Supply Cable

1

Rotor

1

20360104

Allen Wrench

1

50133376

CD with Instruction Manual

1

Check

If any parts are missing, please contact your nearest Thermo Fisher Scientific representative. This symbol refers to general hazards. WARNING means that injuries or material damage or contamination could occur. CAUTION means that material damage could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. This symbol refers to hot surfaces Observe the information contained in the instruction manual to keep yourself and your environment safe.

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P Intended Use

Intended Use • This centrifuge is a laboratory product designed to separate components by generation of Relative Centrifugal Force. It separates human samples (e.g. blood, urine and other body fluids) collected in appropriate containers, either alone or after addition of reagents or other additives. • As general purpose centrifuge, it is designed to also run other containers filled with chemicals, environmental samples and other non-human body samples. • Together with the hematocrit rotor 75003473 the centrifuge can be used as an in-vitro diagnostics device to determine the haematicrit value by using centrifugation. Apply DIN 58933 to determine the hematocit value. • The centrifuge is to be used for separating materials of different density or particle size suspended in a liquid. g

Maximum sample density at maximum speed: 1.2 ---------3 cm

Accident Prevention WARNING If a hazardious situation occurs, turn off the power supply to the centrifuge and leave the area immediately.

WARNING In case of a rotor failure the centrifuge can be damanged. The cooling agent can escape. Ventilate the room well and leave it. Inform Customer Service. Prerequisite for the safe operation of the Heraeus Fresco 17 / 21 is a work environment in compliance with standards, directives and trade association safety regulations and proper instruction of the user. The safety regulations contain the following basic recommendations: • Maintain a radius of at least 30 cm around the centrifuge. Remaining risk: Inproper use can cause damages, contamincation, and injuries with fatal consequences. • Implementation of special measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it is running. • The mains plug must be freely accessible at all times. Pull out the power supply plug or disconnect the power supply in an emergency.

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P Precautions

Precautions In order to ensure safe operation of the Heraeus Fresco 17 / 21, the following general safety regulations must be followed: • The centrifuge should be operated by trained specialists only. • The centrifuge is to be used for its intended use only. • While handling centrifuge, rotor, and samples you must wear laboratory clothing (e.g. gloves). • Plug the centrifuge only into sockets which have been properly grounded. • Do not move the centrifuge while it is running. • Do not lean on the centrifuge. • Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific. Exceptions to this rule are commercially available glass or plastic centrifuge tubes, provided they have been approved for the speed or the RCF value of the rotor. • Do not use rotors which show any signs of corrosion and/or cracks. • Do not touch the mechanical components of the rotor and do not make any changes to the mechanical components. • Use only with rotors which have been properly installed. Follow the instructions in section “Rotor Installation” on page 4-2. • Use only with rotors which have been loaded properly. Follow the instructions given in the rotor manual. • Never overload the rotor. Follow the instructions given in the rotor manual. • Never open the lid until the rotor has come to a complete stop and this has been confirmed in the display. • The lid emergency release may be used in emergencies only to recover the samples from the centrifuge, e.g. during a power failure (see section “Mechanical Emergency Door Release” on page 6-2). • Never use the centrifuge if parts of its cover panels are damaged or missing. • Do not touch the electronic components of the centrifuge or alter any electronic or mechanical components. • Please observe the safety instructions.

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P Precautions

Please pay particular attention to the following aspects: • Location: well-ventilated environment, set-up on a level and rigid surface with adequate load-bearing capacity. • Rotor installation:Check that the rotor is locked properly into place before operating the centrifuge. • Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts and rotor chamber have to be cleaned carefully. • Always balance the samples.

Centrifuging hazardous substances: • Do not centrifuge explosive or flammable materials or substances which could react violently with one another. • The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone environment. • Do not centrifuge inflammable substances. Remaining risk: Inproper use can cause damages, contamincation, and injuries with fatal consequences. • Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety precautions. When centrifuging microbiological samples from the Risk Group II (according to the Biosafety Manual" of the World Health Organization (WHO)), aerosol-tight biological seals have to be used. For materials in a higher risk group, extra safety measures have to be taken. • If toxins or pathogenic substances have gotten into the centrifuge or its parts, appropriate disinfection measures have to be taken (see “Disinfection” on page 5-4). Remaining risk: Inproper use can cause damages, contamincation, and injuries with fatal consequences. • Highly corrosive substances which can cause material damage and impair the mechanical stability of the rotor, should only be centrifuged in corresponding protective tubes.

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Introduction and Description Contents

• “Characteristics of the Heraeus Fresco 17 / 21” on page 1-2 • “Technical Data” on page 1-3 • “Directives, Standards and Guidelines” on page 1-4 • “Functions and Features” on page 1-5 • “Mains Supply” on page 1-5 • “Rotor Selection” on page 1-6

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1 Introduction and Description Characteristics of the Heraeus Fresco 17 / 21

Characteristics of the Heraeus Fresco 17 / 21 You can use various rotors with commercially available containers. The set speed is reached within seconds. The maintenance-free induction motor ensures quiet and low-vibration operation even at high speeds, and guarantees a very long lifetime. The user-friendly control panel makes it easy to pre-set the speed, RCF value, running time, and temperature. You can choose between the display of speed and RCF or the entry mode. These settings can be changed even while the centrifuge is running. With the help of the

key you can also centrifuge a sample for just a few seconds, if called for.

The Heraeus Fresco 17 / 21 is equipped with various safety features: • The housing is made of high-impact resistant plastic and the interior of armour steel. • The lid is equipped with a view port and a lock. • The lid of the centrifuge can only be opened while the centrifuge is switched on and the rotor has come to a complete stop. The centrifuge cannot be started until the lid has been closed properly. • An electronic imbalance recognition in ventilated centrifuges. • For emergencies only, e.g. during power failures (see “Mechanical Emergency Door Release” on page 6-2)

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1 Introduction and Description Technical Data

Technical Data The technical data of the Heraeus Fresco 17 / 21 is listed in the following table. Table 1-1. Technical Data Heraeus Fresco 17 Feature

Value

Environmental Conditions

-Use in interior Spaces -Altitudes of up to 2,000 m above Sea Level -Max. relative Humidity 80% up to 31°C; Decreasing linearly up to 50% relative Humidity at 40°C

Permissible Ambient Temperature during Operation

+5°C bis +40°C

+5°C bis +40°C

+5°C bis +40°C

Permissible Ambient Temperature during Storage and Shipping

+2°C bis +50°C

+2°C bis +50°C

+2°C bis +50°C

Overvoltage Category

II

II

II

Pollution Degree

2

2

2

Heat Dissipation

Heraeus Fresco 17

Heraeus Fresco 17

Heraeus Fresco 17

230 V

120 V

100 V

614 BTU/h

614 BTU/h

580 BTU/h

IP

20

20

20

Run Time

unlimited

unlimited

unlimited

Max Speed nmax

13 300 rpm (Depending on Rotor)

13 300 rpm (Depending on Rotor)

13 300 rpm (Depending on Rotor)

Min Speed nmin

300 rpm

300 rpm

300 rpm

Maximum RCF Value at nmax

17 000 x g

17 000 x g

17 000 x g

Maximum Kinetic Energy

<1,90 kNm

<1,90 kNm

<1,90 kNm

Noise Level at maximum Speed

< 56 dB (A)

< 56 dB (A)

< 56 dB (A)

Hight Width Depth

230 mm 240 mm 350 mm

230 mm 240 mm 350 mm

230 mm 240 mm 350 mm

Weight without Rotor

10.5 kg

10.5 kg

10.5 kg

Dimensions

Table 1-2. Technical Data Heraeus Fresco 21 Feature

Value

Environmental Conditions

-Use in interior Spaces -Altitudes of up to 2,000 m above Sea Level -Max. relative Humidity 80% up to 31°C; Decreasing linearly up to 50% relative Humidity at 40°C

Thermo Scientific

Permissible Ambient Temperature during Operation

+5°C bis +40°C

+5°C bis +40°C

+5°C bis +40°C

Permissible Ambient Temperature during Storage and Shipping

+2°C bis +50°C

+2°C bis +50°C

+2°C bis +50°C

Heraeus Fresco 17 / 21

1-3

1 Introduction and Description Directives, Standards and Guidelines

Table 1-2. Technical Data Heraeus Fresco 21 Feature

Value

Overvoltage Category

II

II

II

Pollution Degree

2

2

2

Heat Dissipation

Heraeus Fresco 21

Heraeus Fresco 21

Heraeus Fresco 21

230 V

120 V

100 V

785 BTU/h

751 BTU/h

785 BTU/h

IP

20

20

20

Run Time

unlimited

unlimited

unlimited

Max Speed nmax

14 800 rpm (Depending on Rotor)

14 800 rpm (Depending on Rotor)

14 800 rpm (Depending on Rotor)

Min Speed nmin

300 rpm

300 rpm

300 rpm

Maximum RCF Value at nmax

21 100 x g

21 100 x g

21 100 x g

Maximum Kinetic Energy

<2,35 kNm

<2,35 kNm

<2,35 kNm

Noise Level at maximum Speed

< 50 dB (A)

< 50 dB (A)

< 50 dB (A)

Hight Width Depth

230 mm 240 mm 350 mm

230 mm 240 mm 350 mm

230 mm 240 mm 350 mm

Weight without Rotor

10.5 kg

10.5 kg

10.5 kg

Dimensions

Directives, Standards and Guidelines Table 1-3. Directives, Standards and Guidelines Tension / Frequency 230V 50Hz/60Hz,

Marked

Produced and inspected according to the following standards and guidelines

EC:

EN 61010-1, 2nd Edition

98/79/EC In-vitro-Diagnostica (IvD)

EN 61010-2-020, 2nd Edition

2006/42/EC Machine Directive

EN 61010-2-101

2004/108/EC EMC Directive

EN 61326-1

2006/95/EC Low Voltage Directive

EN 61326-2-6 EN 55011B EN 61000-6-2 EN ISO 13485

100V 50Hz

cCSAus:

UL Std. No. UL 61010-1, 2nd Edition CAN/CSA-C22.2 No. 61010-1-04 CAN/CSA-C22.2 No. 61010-2-020-09 Part 2-020: IEC 61010-2-020 2nd Edition (Pollution degree 2, Overvoltage category II) IEC 61010-2-101

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1 Introduction and Description Functions and Features

Functions and Features The following table contains an overview of the electrical connection data for the Heraeus Fresco 17 / 21. Table 1-4. Functions and Features Component / Function

Description / Features

Structure / Housing

Sheet steel chassis with attached plastic housing and steel reinforcement

Rotor Chamber

Plastic Up to 48 ml of spilled liquids are retained in the chamber and cannot enter the instrument.

Drive

Induction drive without carbon brushes

Keys and Display

Easy-to-clean keypad and display surface

Controls

Microprocessor-controlled

Internal Memory

Most recent data is saved

Functions

RCF-selection, temperature control, pretemp

Imbalance Recognition

Electronic in ventilated centrifuges

Door Lock

Automatic door closing and locking starting from an initial hold position

Mains Supply The following table contains an overview of the electrical connection data for the der Heraeus Fresco 17 / 21.This data is to be taken into consideration when selecting the mains connection socket. Table 1-5. Electrical connection data Cat.

Mains voltage

Frequency

Rated current

Power consumption

Equipment fuse

Building fuse

75002420

FRESCO 17

230 V

50 / 60 Hz

1.9A

330 W

4A, 2-pole circuit breaker

16 AT

75002421

FRESCO 17

120 V

60 Hz

2.2 A

330 W

8A, 2-pole circuit breaker

15 AT

75002422

FRESCO 17

100 V

60 Hz

2.4 A

330 W

8A, 2-pole circuit breaker

16 AT

75002425

Fresco 21

230 V

50 / 60 Hz

2.2 A

370 W

4A, 2-pole circuit breaker

16 AT

75002421

Fresco 26

120 V

60 Hz

4.4 A

370 W

8A, 2-pole circuit breaker

15 AT

75002422

Fresco 27

100 V

60 Hz

5.1 A

360 W

8A, 2-pole circuit breaker

16 AT

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1-5

1 Introduction and Description Rotor Selection

Rotor Selection The Heraeus Fresco 17 / 21 is supplied with a rotor Various rotors are available to choose from. Microliter 24 x 2

7500 3424

Microliter 36 x 0.5

7500 3436

Dual Rotor 18x2 / 0.5 ml

7500 3418

PCR Rotor 4x8

7500 3440

PCR Rotor 8x8

7500 3489

Hematocrit Rotor

7500 3473

The technical data of the rotors and the corresponding adapters and reduction sleeves for various commercially available containers can be found in the corresponding rotor operating manuals. For more information visit our website at: www.thermoscientific.com/centrifuge.

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Before Use Contents

• “Before Setting up” on page 2-2 • “Transporting the Centrifuge” on page 2-2 • “Location” on page 2-2 • “Aligning the Centrifuge” on page 2-3 • “Mains Connection” on page 2-3 • “Storage” on page 2-4

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Heraeus Fresco 17 / 21

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2 Before Use Before Setting up

Before Setting up 1. Check the centrifuge and the packaging for any shipping damage. Inform the shipping company and Thermo Fisher Scientific immediately if any damage is discovered. 2. Remove the packaging. 3. Remove the rotor transport protection. The cardboard shall prevent damages due to impacts. 4. Check the order for completeness (see “Scope of Supply” auf Seite iii). If the order is incomplete, please contact Thermo Fisher Scientific.

Transporting the Centrifuge • Due to its weight (see “Technical Data” on page 1-3), the centrifuge should be carried by several people. • Always lift the centrifuge at both sides on the bottom plates.

Figure 2-1. Lifting the centrifuge at both sides.

• The centrifuge can get damaged due to impacts.

Location The centrifuge should only be operated indoors. The set-up location must fulfil the following requirements: • A safety zone of at least 30 cm must be maintained around the centrifuge. People and hazardous substances must be kept out of the safety zone while centrifuging. • The supporting structure must be stable and free of resonance, for example a level laboratory bench. • The supporting structure must be suitable for horizontal setup of the centrifuge. • The centrifuge should not be exposed to heat and strong sunlight. WARNING UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.

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2 Before Use Aligning the Centrifuge

• The set-up location must be well-ventilated at all times.

Aligning the Centrifuge The horizontal alignment of the centrifuge must be checked every time after moving it to a different location. The supporting structure must be suitable for horizontal setup of the centrifuge. CAUTION If the centrifuge is not level, imbalances can occur and the centrifuge can be damaged. Do not place anything under the feet to level the centrifuge.

Mains Connection

Main Power Switch Mains plug Figure 2-2. Mains Connection 1. Turn off the power supply switch on the back (press "0"). 2. Plug the centrifuge into grounded electrical sockets only. Note The centrifuge must be connected directly to a socket. Extensions and conneters are not allowed. 3. Check whether the cable complies with the safety standards of your country. 4. Make sure that the voltage and frequency correspond to the figures on the rating plate. Establish the connection to the power supply with the connecting cable.

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2 Before Use Storage

Storage • Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected and decontaminated. • Store the centrifuge in a clean, dust-free location. • Be sure to place the centrifuge on its feet. • Avoid direct sunlight.

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