Heraeus Pico 17-21 Instruction Manual Sept 2012

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©2013 Thermo Fisher Scientific Inc. All rights reserved. HERAEUS is a registered trademark of Heraeus Holding GmbH licensed to Thermo Fisher Scientific. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd. Hypaque is a registered trademark of Amersham Health As. RULON A and Tygon are registered trademarks of Saint-Gobain Performance Plastics. Alconox is a registered trademark of Alconox. Ficoll is a registered trademark of GE Healthcare. Haemo-Sol is a registered trademark of Haemo-Sol. Triton X-100 is a registered trademark of Sigma-Aldrich Co. LLC. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Manufacturer: Thermo Fisher Scientific Robert-Bosch-Straße 1 D - 63505 Langenselbold Germany

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T Table of Contents Preface ... iii Scope of Supply ... iii Intended Use ...iv Accident Prevention ...iv Precautions ...v

Thermo Scientific

Chapter 1

Introduction and Description ... 1-1 Characteristics of the Heraeus Pico 17 / 21 ... 1-2 Technical Data ... 1-2 Directives, Standards and Guidelines ... 1-4 Functions and Features ... 1-4 Mains Supply ... 1-5 Rotor Selection ... 1-5

Chapter 2

Before Use ... 2-1 Before Setting up ... 2-2 Transporting the Centrifuge ... 2-2 Location ... 2-2 Leveling the Centrifuge ... 2-3 Mains Connection ... 2-3 Storage ... 2-4

Chapter 3

Control Panel ... 3-1 Control Panel ... 3-2 Keys ... 3-2

Chapter 4

Operation ... 4-1 Switch on Centrifuge ... 4-2 Lid Opening ... 4-2 Close Door ... 4-2 Rotor Installation ... 4-2 Entering Parameters ... 4-4 Centrifugation ... 4-6 Short-term Centrifugation ... 4-7 Removing the Rotor ... 4-8 Aerosol-tight Rotors ... 4-8 Audible Alarm ... 4-8 Turning off the Centrifuge ... 4-9

Chapter 5

Maintenance and Care ... 5-1

Heraeus Pico 17 / 21

i

Inhalt

Cleaning Intervals ... 5-2 Cleaning ... 5-2 Disinfection ... 5-3 Decontamination ... 5-4 Autoclaving ... 5-5 Service of Thermo Fisher Scientific ... 5-5 Shipping and Deposing of Accessories ... 5-6 Chapter 6

Troubleshooting ... 6-1 Mechanical Emergency Door Release ... 6-2 Troubleshooting by User ... 6-3 When to contact a Service Technician ... 6-6

Chemical Compatibility Chart ...A-1 Contact Information ...B-1

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P

Preface Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions. The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is forbidden to copy or pass on this information without explicit approval. Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty.

Scope of Supply Article Number

Quantity Centrifuge Heraeus Pico 17 / 21

1

Power supply cable

1

Rotor

1

20360104

Square box wrench

1

50134706

CD with Manual

1

Check

If any parts are missing, please contact your nearest Thermo Fisher Scientific representative. This symbol refers to general hazards. WARNING means that injuries or material damage or contamination could occur. CAUTION means that material damage could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. This symbol refers to hot surfaces. Observe the information contained in the instruction manual to keep yourself and your environment safe.

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P Intended Use

Intended Use • This centrifuge is a laboratory product designed to separate components by generation of a relative centrifugal force. It separates human samples (e.g. blood, urine and other body fluids) collected in appropriate containers, either alone or after addition of reagents or other additives. • As general-purpose centrifuge, it is designed to also run other containers filled with chemicals, environmental samples and other non-human body samples. • Together with the hematocrit rotor 75003473 the centrifuge can be used as an in-vitro diagnostics device to define the hematocrit value by centrifugation. Apply DIN 58933 to define the hematocrit value. • The centrifuge is to be used for separating materials of different density or particle size suspended in a liquid. g

Maximum sample density at maximum speed: 1.2 ---------3 cm

Accident Prevention WARNING If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area immediately.

WARNING In case of rotor failure the centrifuge can be damaged. The coolant can escape. Ventilate the room well and leave it. Inform customer service.

WARNING Plug the centrifuge only into sockets which have been properly grounded.

Prerequisite for the safe operation of the Heraeus Pico 17 / 21 is a work environment in compliance with standards, directives and trade association safety regulations and proper instruction of the user. The safety regulations contain the following basic recommendations: • Maintain a radius of at least 30 cm around the centrifuge. • Implementation of special measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it is running. • The mains plug must be freely accessible at all times. Pull out the power supply plug or disconnect the power supply in an emergency.

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P Precautions

Precautions In order to ensure safe operation of the Heraeus Fresco 17 / 21, the following general safety regulations must be followed: • The centrifuge should be operated by trained specialists only. • The centrifuge is to be used for its intended use only. • While handling centrifuge, rotor, and samples you must wear laboratory clothing (e.g. gloves). • Do not move the centrifuge while it is running. • Do not lean on the centrifuge. • Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific. Exceptions to this rule are commercially available glass or plastic centrifuge tubes, provided they have been approved for the speed or the RCF value of the rotor. • Do not use rotors which show any signs of corrosion and/or cracks. • Do not touch the mechanical components of the rotor and do not make any changes to the mechanical components. • Use only with rotors which have been properly installed. Follow the instructions in section “Rotor Installation” on page 4-2. • Use only with rotors which have been loaded properly. Follow the instructions given in the rotor manual. • Never overload the rotor. Follow the instructions given in the rotor manual. • Never open the lid until the rotor has come to a complete stop and this has been confirmed in the display. • The lid emergency release may be used in emergencies only to recover the samples from the centrifuge, e.g. during a power failure (see section “Mechanical Emergency Door Release” on page 6-2). • Never use the centrifuge if parts of its cover panels are damaged or missing. • Do not touch the electronic components of the centrifuge or alter any electronic or mechanical components. • Please observe the safety instructions.

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P Precautions

Please pay particular attention to the following aspects: • Location: well-ventilated environment, set-up on a level and rigid surface with adequate load-bearing capacity. • Rotor installation: make sure the rotor is locked properly into place before operating the centrifuge. • Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts and vessel have to be cleaned carefully. • Always balance the samples.

Centrifuging hazardous substances: • Do not centrifuge explosive or flammable materials or substances which could react violently with one another. • The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone environment. • Do not centrifuge inflammable substances. Remaining risk: Improper use can cause damages, contamination, and injurieswith fatal consequences. • Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety precautions. When centrifuging microbiological samples from the Risk Group II (according to the Bio-safety Manual" of the World Health Organization (WHO)), aerosol-tight biological seals have to be used. For materials in a higher risk group, extra safety measures have to be taken. • If toxins or pathogenic substances have gotten into the centrifuge or its parts, appropriate disinfection measures have to be taken (see “Disinfection” on page 5-3). Remaining risk: Improper use can cause damages, contamination, and injurieswith fatal consequences. • Highly corrosive substances which can cause material damage and impair the mechanical stability of the rotor, should only be centrifuged in corresponding protective tubes.

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1

Introduction and Description Contents

• “Characteristics of the Heraeus Pico 17 / 21” on page 1-2 • “Technical Data” on page 1-2 • “Directives, Standards and Guidelines” on page 1-4 • “Functions and Features” on page 1-4 • “Mains Supply” on page 1-5 • “Rotor Selection” on page 1-5

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Heraeus Pico 17 / 21

1-1

1 Introduction and Description Characteristics of the Heraeus Pico 17 / 21

Characteristics of the Heraeus Pico 17 / 21 Several rotors with a wide range of tubes can be used. The set speed is reached within seconds. The maintenance-free induction motor ensures quiet and low-vibration operation even at high speeds, and guarantees a very long lifetime. The user-friendly control panel makes it easy to pre-set the speed, RCF value, run time, and temperature. You can choose between the display of speed and RCF or the entry mode. These settings can be changed even while the centrifuge is running. With the help of the

key , you can also centrifuge a sample for just a few seconds, if called for.

The Heraeus Pico 17 / 21 is equipped with various safety features: • The housing is made of impact-resistant plastic and the interior of armor steel. • The door is equipped with a view port and a lock. • The centrifuge door can only be opened while the centrifuge is switched on and the rotor has come to a complete stop. The centrifuge cannot be started until the door has been closed properly. • Door emergency release: For emergencies only, e.g. during power failures (see “Mechanical Emergency Door Release” on page 6-2)

Technical Data The technical data of the Heraeus Pico 17 / 21 is listed in the following table. Table 1-1. Technical data Heraeus Pico 17 Feature

Value

Environmental Conditions

-Use in interior Spaces -Altitudes of up to 2,000 m above Sea Level -Max. relative Humidity 80% up to 31°C; decreasing linearly up to 50% relative Humidity at 40°C.

1-2

Permissible Ambient Temperature during Operation

+5°C to +40°C

+5°C to +40°C

+5°C to +40°C

Permissible Ambient Temperature during Storage and Transport

-10°C to +50°C

-10°C to +50°C

-10°C to +50°C

Overvoltage Category

II

II

II

Pollution Degree

2

2

2

Heat Dissipation

Heraeus Pico 17

Heraeus Pico 17

Heraeus Pico 17

230 V

120 V

100 V

614 BTU/h

614 BTU/h

580 BTU/h

IP

20

20

20

Run Time

unlimited

unlimited

unlimited

Heraeus Pico 17 / 21

Thermo Scientific

1 Introduction and Description Technical Data

Table 1-1. Technical data Heraeus Pico 17 Feature

Value

Max Speed nmax

13 300 rpm (depending on the Rotor)

13 300 rpm (depending on the Rotor)

13 300 rpm (depending on the Rotor)

Min Speed nmin

300 rpm

300 rpm

300 rpm

Maximum RCF-Value at nmax

17,000 x g

17,000 x g

17,000 x g

Maximum Kinetic Energy

<1.90 kNm

<1.90 kNm

<1.90 kNm

Noise Level at maximum Speed

< 56 dB (A)

< 56 dB (A)

< 56 dB (A)

Height Width Depth

230 mm 240 mm 350 mm

230 mm 240 mm 350 mm

230 mm 240 mm 350 mm

Weight with emptyRotor

10.5 kg

10.5 kg

10.5 kg

Dimensions

Table 1-2. Technical data Heraeus Pico 21 Feature

Value

Environmental Conditions

-Use in interior Spaces -Altitudes of up to 2,000 m above Sea Level -max. relative humidity 80% up to 31°C; decreasing linearly up to 50% relative humidity at 40°C.

Permissible Ambient Temperature during Operation

+5°C to +40°C

+5°C to +40°C

+5°C to +40°C

Permissible Ambient Temperature during Storage and Transport

-10°C to +50°C

-10°C to +50°C

-10°C to +50°C

Overvoltage Category

II

II

II

Pollution Degree

2

2

2

Heat Dissipation

Heraeus Pico 21

Heraeus Pico 21

Heraeus Pico 21

230 V

120 V

100 V

785 BTU/h

751 BTU/h

785 BTU/h

IP

20

20

20

Run Time

unlimited

unlimited

unlimited

Max Speed nmax

14 800 rpm (depending on the Rotor)

14 800 rpm (depending on the Rotor)

14 800 rpm (depending on the Rotor)

Min Speed nmin

300 rpm

300 rpm

300 rpm

Maximum RCF-Value at nmax

21,100 x g

21,100 x g

21,100 x g

Maximum Kinetic Energy

<2.35 kNm

<2.35 kNm

<2.35 kNm

Noise level at maximum Speed

< 56 dB (A)

< 56 dB (A)

< 56 dB (A)

Dimensions

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1 Introduction and Description Directives, Standards and Guidelines

Table 1-2. Technical data Heraeus Pico 21 Feature

Value

Height Width Depth Weight with empty Rotor

230 mm 240 mm 350 mm

230 mm 240 mm 350 mm

230 mm 240 mm 350 mm

10.5 kg

10.5 kg

10.5 kg

Directives, Standards and Guidelines Table 1-3. Directives, Standards and Guidelines Tension / Frequency 230V 50Hz/60Hz,

Marking

Produced and inspected according to the following standards and guidelines

CE:

EN 61010-1, 2nd Edition

98/79/EC In-vitro-Diagnostika (IvD)

EN 61010-2-020, 2nd Edition

2006/42/EC Machine Directive:

EN 61010-2-101

2004/108/EC EMC Directive

EN 61326-1

2006/95/EC Low Voltage Directive:

EN 61326-2-6 EN 55011B EN 61000-6-2 EN ISO 13485

100V 50Hz

cCSAus:

UL Std. No. 61010-1 (2nd Edition) /CSA-C22.2 No. 61010-1, -04nd Edition CAN/CSA-C22.2 No. 61010-2-020-09 Part 2-020: IEC61010-2-020 (2nd Edition) (Pollution degree 2, Overvoltage category II) IEC 61010-2-101

Functions and Features The following table gives an overview of the important functional and performance characteristics of the Heraeus Pico 17 / 21. Table 1-4. Functions and Features

1-4

Component / Function

Description / Features

Structure / Housing

Sheet Metal with attached Plastic Housing and Steel Chamber

Rotor Chamber

Plastic Up to 48 ml of spilled Liquid is retained in the Chamber and cannot enter the Instrument.

Drive

Induction Drive without Carbon Brushes

Keys and Display

Easy-to-clean Keypad and Display Surface

Control

Microprocessor-controlled

Internal Memory

The most recent Data is saved

Heraeus Pico 17 / 21

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1 Introduction and Description Mains Supply

Table 1-4. Functions and Features Component / Function

Description / Features

Functions

RCF, Temperature, and pre-temp Selection

Door Lock

Automatic Door Closing and Locking starting from an initial hold Position

Mains Supply The following table contains an overview of the electrical connection data for the Heraeus Pico 17 / 21. This data is to be taken into consideration when selecting the mains connection socket. Table 1-5. Electrical connection data Cat.

Mains Voltage

Frequency

Rated Current

Power Consumption

Equipment Fuse

Building Fuse

75002410

PICO 17

230 V

50 / 60 Hz

1.4 A

180 W

4 A, 2-pole circuit breaker

16 AT

75002411

PICO 17

120 V

60 Hz

2.6 A

180 W

8 A, 2-pole circuit breaker

15 AT

75002412

PICO 17

100 V

60 Hz

2.9 A

170 W

8 A, 2-pole circuit breaker

16 AT

75002415

PICO 21

230 V

50 / 60 Hz

1.7 A

230 W

4 A, 2-pole circuit breaker

16 AT

75002416

PICO 21

120 V

60 Hz

3.4 A

220 W

8 A, 2-pole circuit breaker

15 AT

75002417

PICO 21

100 V

60 Hz

3.9 A

230 W

8 A, 2-pole circuit breaker

16 AT

Rotor Selection The Heraeus Pico 17 / 21 is is supplied with a rotor. Various rotors are available to choose from. Microliter 24 x 2

7500 3424

Microliter 36 x 0.5

7500 3436

Dual Rotor 18 x 2 / 0.5

7500 3418

PCR Rotor 4x8

7500 3440

PCR Rotor 8x8

7500 3489

Hematocrit Rotor

7500 3473

The technical data of the rotors and the corresponding adapters and reduction sleeves for various commercially available containers can be found in the corresponding rotor operating manuals. For more information visit our website at: http://www.thermo.com

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2

Before Use Contents

• “Before Setting up” on page 2-2 • “Transporting the Centrifuge” on page 2-2 • “Location” on page 2-2 • “Leveling the Centrifuge” on page 2-3 • “Mains Connection” on page 2-3 • “Storage” on page 2-4

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2-1

2 Before Use Before Setting up

Before Setting up 1. Check the centrifuge and the packaging for any shipping damage. Inform the shipping company and Thermo Fisher Scientific immediately if any damage is discovered. 2. Remove the packaging. 3. Remove the rotor transport protection. The cardboard is suppose to prevent damage due to impact. 4. Check the order for completeness (see “Scope of Supply” on page iii). If the order is incomplete, please contact Thermo Fisher Scientific.

Transporting the Centrifuge WARNING Always lift the centrifuge on both sides. Never lift the centrifuge by its front or the back panel. Transport the centrifuge if possible in its packaging and with the rotor transport protection. • Due to its weight (see “Technical Data” on page 1-2), the centrifuge should be carried by several people. • Always lift the centrifuge at both sides on the bottom plate.

Figure 2-1. Lifting the centrifuge at both sides.

• The centrifuge can be damaged by impacts.

Location The centrifuge should only be operated indoors. The set-up location must fulfill the following requirements: • A safety zone of at least 30 cm must be maintained around the centrifuge. People and hazardous substances must be kept out of the safety zone while centrifuging. • The supporting structure must be stable and free of resonance, for example a level laboratory bench. • The supporting structure must be suitable for horizontal setup of the centrifuge.

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2 Before Use Leveling the Centrifuge • The location must be free of grease and dust. • The centrifuge should not be exposed to heat and strong sunlight. WARNING UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories to direct sunlight. • The set-up location must be well-ventilated at all times.

Leveling the Centrifuge The horizontal leveling of the centrifuge must be checked every time after moving it to a different location. The supporting structure must be suitable for horizontal setup of the centrifuge. CAUTION If the centrifuge is not level, imbalances can occur and the centrifuge can be damaged. Do not place anything under the centrifuge to level.

Mains Connection

Main Power Switch Mains plug Figure 2-2. Mains Connection

1. Turn off the power supply switch on the back (press "0"). 2. Plug the centrifuge into grounded electrical sockets only. Note The centrifuge must be connected directly to the socket. Extension cords and multiple sockets are not allowed. 3. Check whether the cable complies with the safety standards of your country.

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2 Before Use Storage

4. Make sure that the voltage and frequency correspond to the figures on the rating plate. Establish the connection to the power supply with the connecting cable.

Storage • Before storing the centrifuge and the accessories it must be cleaned and if necessary disinfected and decontaminated. • Store the centrifuge in a clean, dust-free location. • Be sure to place the centrifuge on its feet. • Avoid direct sunlight.

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Control Panel Contents

• “Control Panel” on page 3-2 • “Keys” on page 3-2

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Heraeus Pico 17 / 21

3-1

3 Control Panel Control Panel

Control Panel In the control panel you find the keys and displays of the centrifuge. The main power switch is located on the left side. All parameters can be selected and changed during operation. Run Time

Speed / RCF Value

Toggle key

PULSE

OPEN

START

STOP

Keys The keys allow user intervention for controlling the operating mode as follows: Key

Display contents Normal start of the centrifuge. Start End run manually. Stop

Open door

Automatic release (possible only when device is switched on); Emergency release (see “Mechanical Emergency Door Release” on page 6-2).

Pulse

By pressing the key the centrifuge starts immediately and accelerates up to the end speed. Releasing the key initiates a stopping process at the highest braking curves. By pressing the key up and down you change the value in thedisplay. If you hold the key pressed, the display changes continuously at first slowly and after a few seconds at an accelerated pace to the higher or lower values. By pressing the key briefly, you increase or decrease the speed in one step. When pressing both arrows the cursor moves to the left.

Changing the display mode

3-2

Heraeus Pico 17 / 21

Use the key to change the display mode. (speed / RCF value, sample / chamber temperature, run time counter from start or preset speed on)

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