Megafuge 8 and 8R Instruction Manual May 2014

Thermo Scientific

Heraeus Megafuge 8 / 8R Centrifuge Instruction Manual 50143328-b • 05 / 2014

WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:

Table of Contents WEEE Compliance... 4 Preface... 7 Items Supplied... 7 Intended Use... 7 Precautions... 8 Preparation... 8 Hazardous Substances... 9 Operating... 9 Maintenance... 9

Symbols used on the centrifuge... 10 Symbols used in the manual... 10

Technical Specifications... 11 Technical Data... 11 Heraeus Megafuge 8... 11 Heraeus Megafuge 8R... 12

Directives, Standards and Guidelines... 13 Mains Supply... 14 Rotor Selection... 14

Transport and Set Up... 15 Before Setting Up... 15 Location... 15 Transporting... 15 Leveling... 16 Mains Connection... 16 Storage... 17 Shipping... 17

Control Panel... 19 Heraeus Megafuge 8... 19 Heraeus Megafuge 8R... 20

Operation... 21 Switching on the Centrifuge... 21 Open the Centrifuge Door... 21 Thermo Scientific

Heraeus Megafuge 8 / 8R Centrifuge | 5

Table of Contents

Close the Centrifuge Door... 21 Rotor Installation... 22 Acceleration / Deceleration Rates... 23 Pre-Selecting Speed/RCF... 24 Running Time Pre-Selection... 24 Continuous Operation... 25 Preselecting the Temperature... 25 Prewarming or Precooling the Centrifuge... 25

Programs... 26 Saving a Program... 26 Selecting a Program... 26

Centrifugation... 26 Maximum Loading... 26 Starting the Centrifuge Run... 27 Imbalance Indicator... 27 Stopping the Centrifuge Run... 28

Short-term Centrifugation... 28 Removing the Rotor... 28 Aerosol-tight Rotors... 29 Switch off Centrifuge... 29

System Menu... 31 Flowchart System Menu... 31

Maintenance and Care... 33 Cleaning Intervals... 33 Basics... 33 Rotor and Accessories Inspection... 33

Cleaning... 34 Cleaning the Filter Mat... 35

Disinfection... 35 Decontamination... 36 Autoclaving... 36 Service of Thermo Fisher Scientific...37 Shipping and Disposal... 37

Troubleshooting... 39 Mechanical Emergency Door Release... 39 Troubleshooting by Guide... 40 When to contact Customer Service... 42

Chemical Compatibility Chart... 43 Index... 49

6 | Heraeus Megafuge 8 / 8R Centrifuge

Thermo Scientific

P

Preface

Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions.

Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty.

Items Supplied Item

Quantity

Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge

1

Power Supply Cable

1

Instruction Manual

1

CD

1

™

™

™

If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.

Intended Use This centrifuge is a laboratory product used to separate substance mixtures of different densities. This centrifuge can become an In-vitro-diagnostics device (Directive 98/79/EC), if used together with the hematocrit rotor and his accessories. The hematocrit value is determined using the instructions written in the instruction manual of the hematocrit rotor. The instructions are based upon the reference method following DIN 58933, allowing to determinate the erythrocyte volume fraction within the blood. This centrifuge has to be operated by trained specialists only.

Thermo Scientific

Heraeus Megafuge 8 / 8R Centrifuge | 7

Precautions

Precautions w

WA R N I N G

Observe the safety instructions. Not following these instructions can cause damage. The centrifuge is to be used for its intended use only. Improper use can cause damages, contamination, and injuries with fatal consequences. The centrifuge should be operated by trained specialists only. In order to ensure safe operation of the Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge, general safety regulations must be followed. Mind the regulations in your country.

Set Up Conditions d

WA R N I N G

ƒƒ Plug the centrifuge only into sockets which have been properly grounded. ƒƒ Turn off the centrifuge at the main switch. The mains plug must be freely accessible at all times. Press the STOP key to shut down the centrifuge. Pull out the power supply plug or disconnect the power supply in an emergency. ƒƒ As safety zone maintain a clear radius of at least 30 cm around the centrifuge. Do not place any dangerous substances within this security zone. ƒƒ Set up in a well-ventilated environment, on a horizontally levelled and rigid surface with adequate load-bearing capacity.

Preparation d

WA R N I N G

ƒƒ It is the obligation of the operator to make sure, that the proper protective clothing is used. Mind the “Laboratory Biosafety Manual” of the World Health Organization (WHO) and the regulations in your country. ƒƒ Do not make any changes to the mechanical components of the rotor. ƒƒ Do not touch the electronic components of the centrifuge nor alter any electronic or mechanical components. ƒƒ Use only with rotors which have been properly installed. Follow the instructions on the Thermo Scientific™ AutoLock™ rotor exchange in section “Rotor Installation” on page 22. ƒƒ Do not use rotors, buckets or accessories which show any signs of removed protective coating, corrosion or cracks. Contact customer service for further advice or inspections. ƒƒ Use only with rotors which have been loaded properly. ƒƒ Never overload the rotor. ƒƒ Always balance the samples. ƒƒ Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific. Exceptions to this rule are commercially available glass or plastic centrifuge labwares, provided they have been designed to fit rotor or adapter cavities and are approved for the speed or the RCF value of the rotor. ƒƒ Make sure the rotor is locked properly into place before operating the centrifuge. ƒƒ Implement measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it is running. ƒƒ If used for foodstuffs, machinery for cosmetics or pharmaceutical products, only use closed or aerosol-tight containers for centrifugation.

8 | Heraeus Megafuge 8 / 8R Centrifuge

Thermo Scientific

Precautions

Hazardous Substances f

WA R N I N G

ƒƒ Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts and vessel have to be cleaned thoroughly. ƒƒ Do not centrifuge explosive or flammable materials or substances. ƒƒ The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone environment. ƒƒ Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety precautions. If centrifuging any hazardous materials mind the “Laboratory Biosafety Manual” of the World Health Organization (WHO) and any local regulations. When centrifuging microbiological samples from the Risk Group II (according to the “Laboratory Biosafety Manual” of the World Health Organization (WHO)), aerosol-tight biological seals have to be used. Look on the internet page of the World Health Organization (www.who.int) for the “Laboratory Biosafety Manual”. For materials in a higher risk group, extra safety measures have to be taken. ƒƒ If toxins or pathogenic substances have contaminated the centrifuge or its parts, appropriate disinfection measures have to be taken (“Disinfection” on page 35). ƒƒ Extreme care should be taken with highly corrosive substances which can cause damage and impair the mechanical stability of the rotor. These should only be centrifuged in fully sealed tubes. ƒƒ If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area immediately.

Operating w

WA R N I N G

ƒƒ Never use the centrifuge if parts of its cover panels are damaged or missing. ƒƒ Never start the centrifuge when the centrifuge door is open. ƒƒ Do not move the centrifuge while it is running. ƒƒ Do not lean on the centrifuge. ƒƒ Do not place anything on top of the centrifuge during a run. ƒƒ Never open the centrifuge door until the rotor has come to a complete stop and this has been confirmed in the display. ƒƒ The emergency door release may be used in emergencies only to recover the samples from the centrifuge, e.g. during a power failure (see section “Mechanical Emergency Door Release” on page 39). Do not open the centrifuge, while it is running. In any case of severe mechanical failure, such as rotor or bucket crash, the centrifuge is not aerosol-tight. In case of rotor failure the centrifuge can be damaged. Leave the room. Inform customer service.

Maintenance w

WA R N I N G

The centrifuge housing is not to be opened by the operator.

Thermo Scientific

Heraeus Megafuge 8 / 8R Centrifuge | 9

Symbols used on the centrifuge

Symbols used on the centrifuge This symbol refers to general hazards. CAUTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur.

This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe.

This symbol refers to information on hazards, described within the manual.

This symbol demands to disconnect mains before transporting or servicing the centrifuge.

This Symbol demands to check, if the rotor is installed correct by lifting it slightly at the handle. See “Rotor Installation” on page 22.

Symbols used in the manual This symbol refers to general hazards. CAUTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur.

This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe.

This symbol refers to electrical hazards.

10 | Heraeus Megafuge 8 / 8R Centrifuge

Thermo Scientific

Technical Specifications Technical Data

1

Heraeus Megafuge 8 Environmental Conditions

Use in interior spaces Altitudes of up to 2000 m above sea level Max. relative humidity 80% up to 31 °C; decreasing linearly to 50% relative humidity at 40 °C

Environmental Conditions during Storage and Shipping

Temperature: -10 °C to +50 °C Humidity: 15% to 85%

Permissible Ambient Temperature during Operation

+2 °C to +35 °C

Heat Dissipation1

120 V, 60 Hz

230 V, 50 / 60 Hz

0.31 kWh/h; 1060 Btu/h; 1120 kJ/h

0.31 kWh/h; 1060 Btu/h; 1120 kJ/h

Overvoltage Category

II

Pollution Degree

2

IP

20

Running Time

99 h 59 min 50 sec, hold

Maximum Speed nmax

16000 rpm

Minimum Speed nmin

300 rpm

Maximum RCF Value at nmax

24328 x g

Noise Level at Maximum Speed1

< 58 dB (A) (measured with a TX-150 rotor); < 61 dB (A) (measured with a MicroClick 24x2 rotor)

Maximum Kinetic Energy

8.12 kJ

Dimensions

Thermo Scientific

Height (open door / closed door)

660 mm / 310 mm

Width

370 mm

Depth

490 mm

Weight2

35 kg

1

Front Side Measurement, 1 m in front of the instrument at 1.6 m height

2

Without Rotor

Heraeus Megafuge 8 / 8R Centrifuge | 11

Chapter 1 | Technical Data

Heraeus Megafuge 8R Environmental Conditions

Use in interior spaces Altitudes of up to 3000 m above sea level Max. relative humidity 80% up to 31 °C; decreasing linearly to 50% relative humidity at 40 °C

Environmental Conditions during Storage and Shipping

Temperature: -10 °C to +50 °C Humidity: 15% to 85%

Permissible Ambient Temperature during Operation

+2 °C to +35 °C

Heat Dissipation1

120 V, 60 Hz

230 V, 50 / 60 Hz

0.35 kWh/h; 1170 Btu/h; 1140 kJ/h

0.35 kWh/h; 1170 Btu/h; 1140 kJ/h

Overvoltage Category

II

Pollution Degree

2

IP

20

Running Time

99 h 59 min 50 sec, hold

Maximum Speed nmax

17850 rpm

Minimum Speed nmin

300 rpm

Maximum RCF Value at nmax

30279 x g

Noise Level at Maximum Speed1

< 56 dB (A) (measured with a TX-150 rotor)

Maximum Kinetic Energy

10.1 kJ

Temperature Setting Range

-10 °C to +40 °C

Dimensions Height (open door / closed door)

700 mm / 320 mm

Width

460 mm

Depth

670 mm

Weight2

74 kg

1

Front Side Measurement, 1 m in front of the instrument at 1.6 m height

2

Without Rotor

12 | Heraeus Megafuge 8 / 8R Centrifuge

Thermo Scientific

Directives, Standards and Guidelines | Chapter 1

Directives, Standards and Guidelines Region

Directive

Standard

Europe

2006/95/EC Low Voltage (LVD)

EN 61010-1, 2nd Edition

2006/42/EC Machinery (MD)

EN 61010-2-101, 2nd Edition

2004/108/EC Electromagnetic Compatibility (EMC)

EN 61326-2-6

230 V, 50 / 60 Hz

98/79/EC In-vitro-Diagnostika (IvD)

EN 61010-2-020, 2nd Edition EN 61326-1 Class B EN ISO 13485

2002/96/EC Waste Electrical and Electronic Equipment (WEEE) 2011/65/EC Restriction of the use of certain hazardous substances in electrical and electronic equipment (RoHS) 1907/2006 Registration, Evaluation, Authorisation, and Restriction of Chemicals including substances of very high concern, SVHC (REACH) North America (USA & Canada) 230 V, 60 Hz 120 V, 60 Hz

FDA Device Class 1 Product code JQC: centrifuges for clinical use

CAN/CSA-C22.2 No. 61010-1-04 UL Std. No. 61010-1, 2nd Edition) CAN/CSA-C22.2 No. 61010-2-02009-Part 2-020 IEC 61010-2-020, 2nd Edition IEC 61010-2-101, 2nd Edition EN ISO 13485

China

IEC 61010-1, 2nd Edition

230 V, 50 / 60 Hz

IEC 61010-2-020, 2nd Edition IEC 61010-2-101, 2nd Edition EN 61326-1 Class B EN 61326-2-6 EN ISO 13485

Thermo Scientific

Heraeus Megafuge 8 / 8R Centrifuge | 13

Chapter 1 | Mains Supply

Mains Supply The following table contains an overview of the electrical connection data. This data is to be taken into consideration, when selecting the mains connection socket. Unit Article No.

Heraeus Megafuge 8

Heraeus Megafuge 8R

75007210

75007211

75007213

75007214

Mains Voltage

120 V

230 V

120 V

230 V

Frequency

60 Hz

50 / 60 Hz

60 Hz

50 / 60 Hz

Rated Current

5A

2A

8A

4A

Power Consumption

310 W

310 W

700 W

750 W

Equipment Fuse

10 AT

5 AT

15 AT

15 AT

Building Fuse

15 AT

16 AT

15 AT

16 AT

Rotor Selection The Thermo Scientific Heraeus Megafuge 8 / 8R Centrifuge is supplied without a rotor. Various rotors are available. If not stated otherwise the rotors can be used in the Thermo Scientific Heraeus Megafuge 8 centrifuge as also in the Thermo Scientific Heraeus Megafuge 8R centrifuge. Part No.

Thermo Scientific Rotors

75005701

TX-150 swinging bucket rotor

75005702

TX-150 round buckets

75005703

TX-150 50mL conical buckets

75005704

TX100S clinical swinging bucket rotor with sealed carriers

75005705

TX100 clinical swinging bucket rotor with carriers

75005706

M10 microplate swinging bucket rotor

75005723

M10 buckets

75005721

M10 sealed buckets

75005600

MT12 microtube swinging bucket rotor

75005709

HIGHConic III fixed angle rotor

75003623

CLINIConic fixed angle rotor

75005715

MicroClick 24x2 microtube rotor

75005719

MicroClick 30x2 microtube rotor

75005720

8x8 PCR Strip rotor

75005733

Hematocrit rotor

75003694

8 x 50 mL Individually Sealed rotor (only usable in the Heraeus Megafuge 8R)

75005765

MicroClick 18x5 microtube rotor (only usable in the Heraeus Megafuge 8R)

For further details on rotor use and safety, additional accessories, adapters and spare parts, please refer to the rotor manual. For more information visit our website at http://www.thermoscientific.com/centrifuges

14 | Heraeus Megafuge 8 / 8R Centrifuge

Thermo Scientific

Transport and Set Up Before Setting Up 1.

2

Check the centrifuge and the packaging for any shipping damage. Inform the shipping company and Thermo Fisher Scientific immediately if any damage is discovered.

2.

Remove the packaging.

3.

Check, if the items supplied are complete. Ssee “Items Supplied” on page 7. If the items supplied are incomplete, please contact Thermo Fisher Scientific.

Location The is to be centrifuge operated indoors. The set-up location must fulfill the following requirements: ƒƒ A safety zone of at least 30 cm must be maintained around the centrifuge. People and hazardous substances must be kept out of this safety zone while centrifuging. ƒƒ The supporting structure must be stable and free of resonance. ƒƒ The supporting structure must be suitable for horizontal setup of the centrifuge. ƒƒ The centrifuge is not to be exposed to heat and strong sunlight.

WA R N I NG UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.

ƒƒ The set-up location must be well-ventilated at all times.

Transporting WA R N I NG Always lift the centrifuge from both sides. Never lift the centrifuge by its front panel, its back panel or at its door. Always remove the rotor before moving the centrifuge.

Thermo Scientific

Heraeus Megafuge 8 / 8R Centrifuge | 15

Chapter 2 | Leveling

Due to its weight, the centrifuge should be carried by at least two people. Always lift the centrifuge at both sides. To prevent possible injuries at least two people should lift and carry the centrifuge by holding it at the bottom from opposite sides.

Transport the centrifuge and accessories upright within the associated packaging, if possible.

NO T I C E Store the original centrifuge packaging. Contact a shipping company or the customer service for the transport. Always remove the rotor before moving the centrifuge. If you do not remove the rotor you might damage the centrifuge drive or centrifuge spindle.

Leveling C A U T I ON If the centrifuge isn’t leveled, imbalances can occur and the centrifuge can be damaged. Do not place anything under the centrifuge feet to level the centrifuge.

The centrifuge is to be placed on horizontal and level supporting structures or benching. Hoirzontal level is to be checked when moving the centrifuge to a new location.

Mains Connection NO T I C E Plug the centrifuge into grounded electrical sockets only.

1.

Turn off the power supply switch.

16 | Heraeus Megafuge 8 / 8R Centrifuge

Thermo Scientific

Storage | Chapter 2

Power supply switch on the backside of the

Power supply switch on the right side of the

Heraeus Megafuge 8

Heraeus Megafuge 8R

2.

Check whether the cable complies with the safety standards of your country.

3.

Make sure that the voltage and frequency correspond to the figures on the rating plate.

4.

Establish the connection to the power supply with the connecting cable.

Storage WA R N I NG When removing the centrifuge and accessories from use if necessary clean, disinfect or decontaminate the entire system. In doubt contact the Thermo Fisher Scientific customer service.

ƒƒ Before storing the centrifuge and the accessories, it must be cleaned and if necessary desinfected and decontaminated. Centrifuge, rotors, buckets and accessories have to be thoroughly dried before storage. ƒƒ Store the centrifuge in a clean, dust-free location. ƒƒ Be sure to place the centrifuge on its feet. ƒƒ Avoid direct sunlight.

Shipping WA R N I NG Before shipping the centrifuge and accessories you have to clean and if necessary disinfect or decontaminate the entire system. In doubt contact the Thermo Fisher Scientific customer service.

Before shipping the centrifuge please bear the following in mind: ƒƒ The centrifuge must be clean and decontaminated. ƒƒ The decontamination must be confirmed in a decontamination certificate. Contact customer service for more details.

Thermo Scientific

Heraeus Megafuge 8 / 8R Centrifuge | 17

3

Control Panel

The control panel contains the keys and displays of the centrifuge (only the power switch is located on the right side (Heraeus Megafuge 8R) or on the backside (Heraeus Megafuge 8) of the device).

Heraeus Megafuge 8 1

11

Thermo Scientific

2

10

3

4

9

5

6

8

7

No.

Function

Display Contents

1

Status

The status of the centrifuge is displayed here.

2

Speed / RCF Value

The speed (rpm) or RCF value (x g) is displayed here.

3

Running Time

The running time is displayed here.

4

Acceleration / Deceleration Profiles

Press the key multiple times to cycle through the available profiles.

5

PULSE Key

Press the PULSE key to immediately start the centrifugation run and accelerate up to the maximal permissible end speed (depending on the used rotor). Releasing the key initiates a stopping process at the highest braking curves.

6

OPEN Key

Press the OPEN key to activate the automatic door release (possible only when device is switched on and when the rotor is fully stopped). “Mechanical Emergency Door Release” on page 39

7

STOP Key

Press the STOP key to manually end the centrifugation run.

8

START Key

Press the START key to start a centrifugation run or to accept the current settings.

9

Arrow Keys

Use these keys in order to modify the displayed value.

10

TOGGLE Key for Speed / RCF Value

Use the TOGGLE key to change the display mode. (Speed / RCF Value).

11

Program Keys

Use the Program Keys to save and load programs. “” on page 25

Heraeus Megafuge 8 / 8R Centrifuge | 19

Chapter 4 | Heraeus Megafuge 8R

Heraeus Megafuge 8R 1

13

2

12

3

4

11

5

6

7

10

9

8

No.

Function

Display Contents

1

Status

The status of the centrifuge is displayed here.

2

Speed / RCF Value

The speed (rpm) or RCF value (x g) is displayed here.

3

Running Time

The running time is displayed here.

4

Temperature

The temperature is displayed here.

5

Acceleration / Deceleration Profiles

Press the key multiple times to cycle through the available profiles.

6

PULSE Key

Press the PULSE key to immediately start the centrifugation run and accelerate up to the maximal permissible end speed (depending on the used rotor). Releasing the key initiates a stopping process at the highest braking curves.

7

OPEN Key

Press the OPEN key to activate the automatic door release (possible only when device is switched on and when the rotor is fully stopped). “Mechanical Emergency Door Release” on page 39

8

STOP Key

Press the STOP key to manually end the centrifugation run.

9

START Key

Press the START key to start a centrifugation run or to accept the current settings.

10

SNOWFLAKE Key

Use the key for prewarming or precooling the centrifuge.

11

Arrow Keys

Use these keys in order to modify the displayed value.

12

TOGGLE Key for Speed / RCF Value

Use the TOGGLE key to change the display mode. (Speed / RCF Value).

13

Program Keys

Use the Program Keys to save and load programs. “” on page 25

20 | Heraeus Megafuge 8 / 8R Centrifuge

Thermo Scientific

Operation

4

NOTE Only display pictures of the Heraeus Megafuge 8R centrifuge are shown. The display of the Heraeus Megafuge 8 is the same only missing the details for temperature.

Switching on the Centrifuge 1.

Turn on the power switch of the device. The device performs a self-check of its software. a.

When the centrifuge door is closed the display shows:

The speed and time displays read “0” and “00:00”; the current temperature inside the rotor chamber is displayed. b.

When the centrifuge door is open the display shows:

The speed and time displays show the pre-set values ; the set temperature inside the rotor chamber is displayed.

Open the Centrifuge Door Press the OPEN key.

Close the Centrifuge Door WA R N I NG Do not reach into the gap between the centrifuge door and the housing. Use the emergency release only for malfunctions and power failures (see “Mechanical Emergency Door Release” on page 39).

Thermo Scientific

Heraeus Megafuge 8 / 8R Centrifuge | 21

Chapter 4 | Rotor Installation

Ensure that the centrifuge platform is clear from objects. Keep hands and objects well clear of the underside and side of the centrifuge door when closing. Close the centrifuge door by pressing down on it lightly in the middle or on both sides of it. The centrifuge door mechanism will click and lock in place. Lids should not be slammed as excessive force may cause damage or disrupt samples.

NO T I C E The centrifuge door should audibly click into place.

Rotor Installation The approved rotors for the Thermo Scientific Heraeus Megafuge 8 / 8R are listed in section “Rotor Selection” on page 14. Use only the rotors from this list in the centrifuge. Permitted accessories are listed in the rotor manuals.

C A U T I ON Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge.

The centrifuge is equipped with an Auto-Lock-locking system. This system is used to automatically lock the rotor to the centrifuge spindle. The rotor does not have to be bolted on to the centrifuge spindle. Proceed as follows: 1.

Open the centrifuge door and if necessary remove any dust, foreign objects or residue from the chamber. Auto-Lock and O-Ring must be clean and undamaged.

1 2

2.

1

Auto-Lock

2

O-Ring

Place the rotor over the centrifuge spindle and lower vertically and slowly down the centrifuge spindle. If necessary a light push may be required to ensure connection. The rotor clicks automatically into place.

22 | Heraeus Megafuge 8 / 8R Centrifuge

Thermo Scientific