Operating Instructions
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Operating Instructions
CO2 incubator HERAcell ® 240 with decontamination routine Sicherheitswerkbank HERAsafe HS 9/12/15/18
Valid: 08.20O2 / 50071613 A
CO2 Incubator HERAcell® 240 Operating Instructions
Copyright© These operating instructions are protected by copyright. Rights resulting thereof, particularly reprint, photomechanical or digital postprocessing or reproduction, even in part, are only allowed with the written consent of Kendro Laboratory Products GmbH. This regulation does not apply to reproductions for in-plant use. The contents of the operating instructions are subject to change without further notice. Concerning translations into foreign languages, the German version of these operating instructions is binding.
Trademarks HERAcell® and Heraeus are registered trademarks of Kendro Laboratory Products GmbH. All other trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers. Kendro Laboratory Products GmbH Heraeusstraße 12-14 D - 63450 Hanau Germany
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CO2 Incubator HERAcell® 240 Operating Instructions
Contents 1. General notes ... 6 1.1 General safety instructions ... 6 1.2 Warranty ... 7 1.3 Explanation of symbols ... 8 1.3.1 Symbols used in the operating instructions ... 8 1.3.2 Symbols used in the quick reference ... 9 1.3.3 Symbols on the device ... 10 1.4 Intended purpose of the device ... 11 1.4.1 Correct use ... 11 1.4.2 Incorrect use ... 11 1.5 Standards and directives ... 12 1.6 Safety notes on gases ... 12 2. Delivery ... 15 2.1 Packaging ... 15 2.2 Components standard equipment ... 15 2.3 Components optional equipment ... 16 2.4 Acceptance inspection ... 16 3. Installation of the device ... 17 3.1 Ambient conditions ... 17 3.2 Room ventilation ... 17 3.3 Space requirements ... 18 3.4 Transport ... 18 3.5 Stacking ... 19 3.6 Retrofitting/Modifications ... 19 3.7 Tray ... 19 4. Description of the device ... 20 4.1 Front view ... 20 4.2 Rear view ... 21 4.3 Safety devices ... 22 4.4 Work space atmosphere ... 22 4.5 ContraCon decontamination routine ... 23 4.6 Sensor system ... 24 4.7 Door switch ... 24 4.8 Supply interface ... 25 4.9 Work space components ... 26 5. Start-up ... 31 5.1 Preparing the work space ... 31 5.2 Installing the shelf system ... 31 5.3 Gas connection ... 34 5.4 Power supply connection ... 35 5.5 RS 232 interface connection ... 36 5.6 Connecting the alarm contact ... 38 6. Handling and control ... 40 6.1 Power switch ... 40 6.2 Operating panel ... 40 6.3 Control self-test ... 41 6.4 Setting the nominal temperature value ... 42 6.5 Setting the O2 nominal value ... 42 3
CO2 Incubator HERAcell® 240 Operating Instructions
Contents 6.6 6.7 6.8 6.9 6.10 6.11 6.12 6.13 6.14
Disabling the O2 control ... 43 Enabling the O2 control ... 44 Setting the CO2 nominal value ... 45 Setting the high/low humidity ... 45 Activating the auto-start routine ... 47 Reading failure codes ... 49 Failure code list ... 52 Resetting the thermal protection ... 53 Setting the drive roller speed ... 53
7. Operation ... 54 7.1 Preparing the device ... 54 7.2 Starting operation ... 55 8. Shut-down ... 56 8.1 Shutting the device down ... 56 9. Cleaning and disinfection ... 56 9.1 Decontamination procedures ... 57 9.2 Wipe/Spray disinfection ... 57 9.3 ContraCon decontamination routine ... 61 9.4 Activating the ContraCon decontamination routine ... 64 9.5 Cancelling the ContraCon decontamination routine ... 64 10. Maintenance ... 65 10.1 Inspections and checks ... 65 10.2 Service intervals ... 65 10.3 Preparing the temperature calibration ... 66 10.4 Temperature calibration procedure ... 67 10.5 Preparing the CO2 calibration ... 68 10.6 CO2 calibration procedure ... 69 10.7 Replacing the sterile filters ... 70 10.8 Replacing the device fuses ... 70 10.9 Replacing the door seal ... 71 11. Spare parts and accessories ... 72 11.1 List of spare parts and accessories ... 72 12. Technical data ... 74 13. Disposal ... 77 14. Principles of good microbiological proceedings ... 78 15. Device log ... 80 16. Certificate of decontamination ... 81 17. Addresses ... 83
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CO2 Incubator HERAcell® 240 Operating Instructions
Figures Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7 Fig. 8 Fig. 9 Fig.10 Fig.11 Fig.12 Fig.13 Fig.14 Fig.15 Fig.16 Fig.17 Fig.18 Fig.19 Fig.20 Fig.21 Fig.22 Fig.23 Fig.24 Fig.25 Fig.26 Fig.27 Fig.28 Fig.29 Fig.30 Fig.31
Device dimensions and minimal distances ... 18 Lift points ... 18 Stacking ... 19 Tray installation ... 19 Front view ... 20 Rear view ... 21 CO2 and O2/N2 sensors ... 24 Door switch ... 24 Supply interfaces ... 25 Work space layout ... 26 Water reservoir, gas humidification ... 27 Bottle turning device ... 28 Speed ranges ... 29 Rear panel openings ... 30 Support rail installation/removal ... 31 Support hook/Insertion sheet/Roller insert installation ... 32 Center strut/gas humidification installation ... 33 Gas supply line installation ... 34 Power supply connection ... 35 RS 232 interface ... 36 Example of external alarm system ... 39 Power switch ... 40 Operating panel functions ... 40 Blower wheel/Cover removal ... 59 ContraCon decontamination routine procedure ... 61 Temperature calibration ... 65 CO2 calibration ... 67 Sterile filter replacement ... 70 Device fuse replacement ... 70 Door seal replacement ... 71 Gas consumption ... 76
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CO2 Incubator HERAcell® 240 Operating Instructions
1.
General notes 1.1
General safety instructions
These operating instructions describe the CO2 incubator HERAcell® 240. The CO2 incubator has been manufactured in keeping with the latest technological developments and is operationally safe. However, the device may present potential hazards, particularly if it is operated by inadequately trained personnel or if it is not used in accordance with the intended purpose. Therefore, the following must be observed to prevent accidents: The CO2 incubator must only be operated by trained and authorized personnel. For personnel operating this device, the operator must prepare written instructions in a reasonable form based on these operating instructions, the safety data sheets, the hygiene regulations and the applicable Technical Guidelines, in particular: which decontamination measures are to be taken for the device and for the accessories used, which safety measures are to be taken when gases and pressurized gas containers are used, which measures are to be taken in case of an accident. Any repairs to the device must only be performed by adequately trained and authorized expert personnel. The contents of the operating instructions are subject to change without further notice. Concerning translations into foreign languages, the German version of these operating instructions is binding. Keep these operating instructions in the vicinity of the device so that safety instructions and important information are always accessible. Should you encounter problems that are not mentioned in these operating instructions, please contact Kendro Laboratory Products GmbH immediately for your own safety. For a list of all international Kendro sales organizations, please refer to the end of these operating instructions. Postal address Germany Kendro Laboratory Products GmbH Heraeusstraße 12-14 D - 63450 Hanau Germany
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Phone Sales Service
+ 49 (0) 1805-536376 + 49 (0) 1805-112110
Fax Sales Service
+ 49 (0) 1805-112114 + 49 (0) 1805-112114
Postal addresses USA Kendro Laboratory Products Inc. 275 Aiken Road Asheville, NC 28804 USA Phone +1 (800) 252-7100 Fax +1 (800) 645-3368 Kendro Laboratory Products L.P. 31 Pecks Lane Newton, CT06470-2337 USA Phone +1 (800) 522-7746 Fax +1(203)270-2166
CO2 Incubator HERAcell® 240 Operating Instructions
1.
General notes 1.2
Warranty
Kendro Laboratory Products warrant the operational safety and the operativeness of the CO2 incubator HERAcell® 240 only under the condition that: the device is operated and serviced exclusively in accordance with its intended purpose and as described in these operating instructions, the device is not modified, only original spare parts and accessories that have been approved by Kendro Laboratory Products are used, inspections and maintenance works are carried out at the specified intervals. The warranty is valid from the date of delivery of the device to the operator.
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CO2 Incubator HERAcell® 240 Operating Instructions
1.
General notes 1.3
Explanation of symbols
1.3.1
Symbols used in the operating instructions: WARNING! is used if non-observance may cause serious or even lethal injuries.
CAUTION! is used if non-observance may cause medium to minor injuries or damage.
NOTE is used for applicational hints and useful information.
Wear safety gloves!
Wear safety goggles!
Harmful liquids!
Electric shock!
Hot surfaces!
Fire hazard!
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CO2 Incubator HERAcell® 240 Operating Instructions
1.
General notes 1.3.2
Symbols used in the quick referenc:
Operational steps to be performed at the device
Display state of device indicators
Turn device on
Turn device off
Open device doors
Leave device doors open for at least 30 seconds
l
Check humidification system water level, refill as necessary, level between 1.8 l (min.) and 4.5 l (max.)
Refill 350 ml water for ContraCon disinfection routine
Initiate auto-start, keep key depressed for at least 5 seconds
Initiate ContraCon decontamination routine, keep key depressed for approx 5 seconds
Set nominal values for temperature, CO2 , O2, and speed
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CO2 Incubator HERAcell® 240 Operating Instructions
1.
General notes
Clean device work space
Close device doors
Remove samples and water from device
Charge device
Note in operating instructions, page ...
1.3.3
Symbols on the device:
CE symbol
VDE - safety-tested
Test certificate for USA/Canada
Observe operating instructions!
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CO2 Incubator HERAcell® 240 Operating Instructions
1.
General notes 1.4
Intended purpose of the device
1.4.1
Correct use
The CO2 incubator HERAcell® 240 is a laboratory device for preparing and cultivating cell and tissue cultures. The device allows the simulation of the special physiological ambient conditions for these cultures due to the exact control of: temperature, CO2 content, O2/N2 content, and the setting of an increased relative humidity. The HERAcell® 240 has been designed for installation and operation in the following fields of application: Laboratories for cytobiological and biotechnological experiments of safety levels L1, L2, and L3. Medical-microbiological laboratories in accordance with DIN 58 956. Laboratories in the central area of clinics and hospitals. The gases required for the incubator (CO2 and/or N2 / O2) are supplied to the device from a separate gas supply system, either from gas cylinders or from a central pressurized gas container. The layout of the gas supply system must ensure that the operating pressure of the gas supply lines can be set to a range between 0.8 bar (min.) to 1 bar (max.) and that the pressure cannot be changed. The CO2 incubator is suited for continuous operation. 1.4.2 Incorrect use: Do not use cell or tissue cultures in the device that are not in accordance with the regulations of safety levels L1, L2, and L3. Do not use tissues, substances or liquids that: are easily ignitible or explosive, release vapors that form combustible or explosive mixtures when exposed to air, release poisons.
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CO2 Incubator HERAcell® 240 Operating Instructions
1.
General notes 1.5
Standards and directives
The device is in accordance with the following standards and guidelines: DIN EN 61010 Low Voltage Guideline 73/23 EWG EMC Guideline 89/336 EWG UVV VBG 20 DIN 12880 Part 1/11.78 The following safety regulations must be observed if the device is operated within the territory of the Federal Republic of Germany: ZH 1/10 ZH 1/119 ZH 1/342 ZH 1/343 ZH 1/598 TRG 280 EC Official Gazette, L 374 Safety data sheets of the gas supplier relevant to the particular characteristics of CO2, O2, and N2. Principles of good microbiological proceedings, notice of the trade association of the German chemical industry. For other countries, the applicable national regulations are binding.
1.6
Safety notes on gases
Carbon dioxide (CO2): As CO2 is rated as a harmful gas, certain safety instructions must be observed when the CO2 incubator is started up and when the device is operated. NOTE Personnel instruction Personnel operating devices with CO2 supply must be instructed about the particularities in the handling of CO2 before starting their work: Correct operation of pressurized gas containers and gas supply systems (e.g. TRG 280), Obligation to report damages and shortcomings in CO2 supply lines, Measures to be taken in case of accidents or failures. These instructions must be repeated in appropriate intervals and must comprise the particular operating instructions of the gas supplier.
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CO2 Incubator HERAcell® 240 Operating Instructions
1.
General notes WARNING - Suffocation hazard! CO2 released in large amounts into the room atmosphere may cause suffocation. If CO2 is released, initiate safety measures immediately! Leave the room immediately and do not allow others to enter the room! Inform security service or fire department! Oxygen (O2): O2 is a gas that promotes combustion and may explode in combination with grease-containing materials. WARNING Oxygen explosion! O2 may explode in combination with oils, greases, and lubricants. If highly compressed oxygen comes in contact with grease- or oil-containing substances, the mixture may explode! For cleaning these device components, use only oil- and grease-free lubricants. Keep all connections and components of the oxygen system free from substances that contain oil, grease, or lubricant! CAUTION Fire hazard! Released oxygen promotes combustion. Do not use open flames in the vicinity of oxygenoperated systems! Do not smoke in the vicinity of oxygen systems. Do not expose the components of an oxygen system to excessive heat.
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CO2 Incubator HERAcell® 240 Operating Instructions
1.
General notes Nitrogen (N2): Nitrogen mixes easily with air. High concentrations of nitrogen reduce the oxygen content in the air. CAUTION Suffocation hazard! N2 released in large amounts into the room atmosphere may cause suffocation due to oxygen deficiency. If N2 is released, initiate safety measures immediately!! Leave the room immediately and do not allow others to enter the room! Inform security service or fire department! NOTE Installation work Any work to supply lines and pressurized gas containers, cylinders or containers used for storing N2, CO2 or O2 must only be carried out by expert personnel using the appropriate tools.
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CO2 Incubator HERAcell® 240 Operating Instructions
2.
Delivery 2.1
Packaging
The CO2 incubator HERAcell® 240 is delivered in a stable packaging box. All packaging materials can be separated and are reusable: Packaging materials Packaging carton Foam elements Pallet Packaging film Packaging ribbons
2.2
Recycled paper Styrofoam (CFC-free) Untreated wood Polyethylene Polypropylene
Components standard equipment CO2 or CO2/O2 incubator with solid glass door
CO2 or CO2/O2 incubator with 6-door gas tight screen (optional)
Quantity of the delivered components
Solid glass door with continuous shelves (standard version)
Version: Solid glass door with split shelves
Version: 6-door gas tight screen with continuous shelves
Version: 6-door gas tight screen with split shelves
Shelf Support rail for shelf Shelf supports for shelf Tray
3 4 6 1
6 6 12 1
3 4 6 1
6 6 12 1
Insert for pressure compensation opening
1
1
1
1
Plug for pipe channel Power supply cable
1 1
1 1
1 1
1 1
Connector, potentialfree contact
1
1
1
1
Spare caps, set CO2 connecting hose Immersion water pump
1 1 1
1 1 1
1 1 1
1 1 1
Open-end wrench, 24 mm
1
1
1
1
Allen wrench 2 mm for blower wheel
1
1
1
1
Allen wrench 3 mm for blower wheel cover
1
1
1
1
Operating instructions Quick reference
1 2
1 2
1 2
1 2
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CO2 Incubator HERAcell® 240 Operating Instructions
2.
Delivery 2.3
Components optional equipment CO2 or CO2/O2 incubator with solid glass door
Quantity of the delivered components
Solid glass door with continuous shelves (standard version)
Version: Solid glass door with split shelves
Version: 6-door gas tight screen with continuous shelves
Version: 6-door gas tight screen with split shelves
1
-
1
-
1
1
1
1
1
1
1
1
Bottle turning device (motor with four rollers, optional) 1, 2 O2 connecting hose set O2 gas humidification system connecting hose set 3 1 2
3
CO2 or CO2/O2 incubator with 6-door gas tight screen (optional)
For bottle turning devices, the version with split shelfs is not available. Each level of a bottle turning device is supplied in a separate box. The boxes (1 to 4) are delivered with the device. O2 sensor and gas humidification set are supplied in a separate box that is placed into the work space during the transport.
2.4
Acceptance inspection
After the device has been delivered, check the delivery immediately for: completeness, possible damages. If damages are detected or if components are missing, please contact the linehauler and Kendro Laboratory Products immediately.
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CO2 Incubator HERAcell® 240 Operating Instructions
3.
Installation of the device 3.1
Ambient conditions
The device must only be operated at locations that meet the particular ambient conditions listed below. Requirements: Draft-free and dry location. The minimal distance to adjacent surfaces must be observed on all sides (see Section 3.3.). The operating room must be equipped with an appropriate room ventilation. Solid, level, fire-proof surface. Vibration-proof substructure (floor stand, lab table) capable of bearing the weight of the device and of accessories (particularly if several devices are stacked). To ensure a constant incubation temperature of 37° C, the ambient temperature must be within a range of +18° C to +33° C. Relative humidity up to 80 % (max.). No direct exposure to sunlight. Devices that produce excessive heat are not allowed near the location of the HERAcell® 240.
3.2
Room ventilation
When CO2/O2/N2 is supplied, the work space of the incubator is slightly pressurized. The pressure is released through the pressure compensation opening into the operating room. As the pressure compensation and any opening of the glass door/gas tight screen during the operation of the device will release very small quantities of CO2/ O2/N2 into the operating room, the room ventilation must be capable of carrying the released gas safely off into the open. In addition, heat dissipating from the device during permanent operation may cause a change in the room climate. Therefore, the HERAcell® 240 must only be installed in rooms with sufficient ventilation. Do not install the device in room recesses without ventilation. The room ventilation should be a technical ventilation that is in accordance with the requirements of ZH 1/119 (Guidelines for laboritories) or some other suited ventilation system with appropriate capacity.
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CO2 Incubator HERAcell® 240 Operating Instructions
3.
Installation of the device 3.3
Space requirements
When installing the device, make sure that the installation and supply connections are freely accessible. The control box at the rear panel of the device may serve as a spacer to adjacent objects. The side distances given are minimal distances. To protect the CO2 incubator against contamination, use a floor stand even if the device is installed near the floor. The height of the floor stand should not fall below 200 mm. Several floor stands and carriers are available as options from Kendro (part numbers see Section 11.1, Spare parts and accessories). NOTE Accessibility of the devices To ensure the accessibility for care and maintenance works, keep larger side and rear distances.
3.4
Transport
For the transport do not lift the device using the doors or components attached to the device (e.g. control box on rear panel) as lift points. NOTE Lift points Lift the device only using the lift points shown in Fig. 2.
Fig. 1 Device dimensions and minimal distances in mm, F = Front
Fig. 2 Lift points
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CO2 Incubator HERAcell® 240 Operating Instructions
3.
Installation of the device 3.5
Stacking
No more than two HERAcell® 240 devices must be stacked on top of each other. An adapter plate [3] is used for the thermal separation of the two devices. The stacking elements [1] and [2] secure the devices on top of each other. If the devices are installed on mobile floor stands, make sure that the rollers are locked by a brake during the operation and that the rollers are facing forward to ensure maximal stability. NOTE Transporting stacked devices The stacking elements are are not connecting elements. Therefore, the transport of stacked devices on sloped surfaces is not allowed.
3.6
Retrofitting/Modifications
The hinges of both the outer door and the glass door can be mounted to either the left or right side of the device. The mounting can also be changed later from the left to the right side or vice versa. The standard version with glass door can also be substituted later with 6 separate gas tight screens.
Fig. 3 Stacking
NOTE Modifications Retrofittings and modifications must only be performed by the Technical Service of Kendro Laboratory Products.
3.7
Tray
After the device has been installed, the tray is inserted between the two guide straps below the bottom of the device. 1. Open the outer door of the device all the way to the stop. 2. Fold the guide straps [1] all the way down. 3. Position tray [4] with vertical edge [2] below cable guide [3]. The quick reference [5] faces the front of the device. 4. Lift tray slightly and tilt it downward. 5. Push tray in until resistance is observed, then push tray in all the way to the stop by pushing onto guide straps.
Fig. 4 Tray installation
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CO2 Incubator HERAcell® 240 Operating Instructions
4.
Description of the device 4.1 [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14]
Front view Stacking elements Plug caps Glass door Measuring cell with blower wheel and sensors Door switch Oxygen sensor (optional) Receptacle for bottle turning device Measuring opening Plug for bottle turning device Outer door Outer door seal, replaceable Stand, height-adjustable Drive roller for bottle turning device Gas humifification (optional)
[15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27]
Water level sensor Insert for bottle turning device (optional) Nameplate Tray, extractable Cylinders Power switch Support rail Shelf Latch, glass door Support hook for shelf Pressure compensation opening with insert Access port with plug Glass door seal, replaceable
Fig. 5 Front view
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