HERAcell and HERAcell 240 Manuals various versions

THE IDEAL CO22 INCUBATOR The Heraeus® HERAcell by Kendro Laboratory Products is the ideal CO2 incubator for cell and tissue cultures, to include development of ova or embryos at or near body temperature. It provides everything needed to protect and grow your samples: ■ Fewer contamination-prone surfaces save time

and prevent culture loss. ■ ContraCon 90 ºC decontamination routine

decontaminates the entire chamber interior and is proven to eliminate mycoplasma. ■ Direct humidification offers the fastest humidity

recovery times and provides optimal growth conditions for cultures.

HERAcell® 240 [240 l (8.4 cu ft) internal volume] 02

Good growth for cultures ContraCon – effective decontamination A feature found only on Heraeus incubators, ContraCon is an automatic decontamination routine that uses moist heat at 90 ºC. In independent tests, the ContraCon routine was proven to completely eliminate mycoplasma.

Consistent culture conditions exist throughout the incubator interior. This is achieved by the air jacket temperature control. In addition, the CO2 sensor ensures long term stability of the incubation atmosphere. A newly developed uppertemperature limit protection ensures exact incubation temperature even in the event of a sensor failure.

A constant surface temperature on all contamination-prone surfaces is ensured. ContraCon’s decontamination efficiency was tested by a GLP approved laboratory and CAMR, Porton Down, UK.

Contamination protection Fewer surfaces means less area for contamination. HERAcells were designed to have fewer contamination-prone surfaces than competitive models.

Cultures are protected from drying out HERAcell uses a new type of water reservoir – one that replaces the standard water tray. This new system provides a high relative humidity (rH) and most importantly, allows rapid humidity recovery times. Compared to common CO2 incubators with water trays, the HERAcell’s humidity recovery time is up to ten times faster.

HERAcell® [150 l (5.3 cu ft) internal volume] 03

DESIGN DETAILS FOR MAXIMUM PROTECTION The reduction of contamination-prone surfaces inside the chamber is the most efficient method of preventing contamination in the long term.

Completely smooth The HERAcell’s inner casing is completely smooth and has rounded corners on all sides. Instead of large shelf fittings, neat grooves provide sufficient support for the shelf system. As a result, the inner casing can be thoroughly and easily cleaned.

Less means more

Compared with common CO2 incubators, all HERAcell surfaces are accessible. Potential sources of contamination, for example plastic fans and filter casings hidden behind ceiling panels, are not present in the HERAcell.

HERAcell components have up to 50% fewer contaminationprone surfaces than common CO2 incubators incorporating large shelf supports and water trays. This means less timeconsuming cleaning and greater contamination protection.

A new type of water reservoir Standard access port Every HERAcell incubator comes with a 42 mm access port to facilitate the installation of appliances within the incubator chamber, that require pass through of electrical cords or tubing outside the incubator. 04

Thanks to the patented tilted inner chamber, the water reservoir is large enough to hold three liters. In addition, the awkward handling and cleaning of a water tray is eliminated. The heated floor underneath the water reservoir provides humidity recovery times that are up to ten times faster than comparable water tray incubators.

Designed for ease-of-use The HERAcell interior including the fan and its cover are made of stainless steel or copper. For improved contamination protection, the use of plastics in the interior is reduced to a minimum. Doors can be set up for left or right handed usage to optimize your work space. All door gaskets can be removed by hand. They have smooth surfaces for easy cleaning.

Easy-to-view controls Two large illuminated displays make parameter control simple. You can simultaneously check and monitor the temperature and the CO2 levels at a glance.

Practical operation The pull-out tray underneath the incubator provides space for storage of supplies and operating instructions, as well as a place for keeping your research notes. It also serves as a convenient shelf during loading and unloading.

For long term incubation HERAcell 240 offers an additional High-Low Humidity Function to reduce the interior humidity from ≥ 95 % rH to 90 % rH.

05

FOR YOUR CULTURES

Protected from drying out

Reduction of contamination-prone surfaces

The ContraCon automatic decontamination routine consistently achieves excellent decontamination at a moist heat of 90 ºC.

The incubator’s high relative humidity and short recovery times prevent the culture media from evaporating.

Compared with other CO2 incubators, HERAcell has up to 2.5 m2 (27 sq ft) less contamination-prone surfaces.

In less than a day, the HERAcell’s entire interior, including all sensors, shelves, fan and the critical water reservoir, are decontaminated. We save you time by not requiring the removal, refitting or separate decontamination of sensors. There is no limit to the number of times that ContraCon can be used for decontamination.

The HERAcell incubator’s inclined/rounded cornered water reservoir provides rapid

There are no concealed or inaccessible areas for contaminants to collect.

4.19 Other CO2 incubators

3.26

2.95

2.68

2.47 1.8 HERAcell

60 64 38

Contamination-prone surfaces (m2)

UK.

Other CO2 incubators

laboratory and CAMR, Porton Down,

15

faces was tested by a GLP approved

HERAcell

ContraCon’s effectiveness on all sur-

Humidity recovery time (min)

108

120

Reliable decontamination with ContraCon

Contaminants tested: ■ Bacillus subtilis ■ Bacillus stearothermophilus (USP 23) ■ Enterococcus faecalis ■ Escherichia coli ■ Pseudomonas aeruginosa

Humidity recovery times – HERAcell compared with common CO2 incubators

Contamination-prone surfaces – HERAcell compared with other CO2 incubators

humidity recovery times even when the door is opened frequently or when low media volumes are used … up to ten times faster than competitive models.

Extended safety features

■ Staphylococcus epidermidis ■ Corynebacterium xerosis ■ Aspergillus niger

A second independent laboratory tested and confirmed ContraCon’s complete elimination of mycoplasma.

06

With water tray incubators, a layer of air between the incubator and tray, slows down the humidity recovery, resulting in high culture media evaporation rates. HERAcell has a heated floor which allows for direct heat transfer to the humidification water.

The unique upper temperature limit protection system not only warns of overheating, but it also maintains the set incubation temperature. The inner glass door of competitive CO2 incubators can accidentally be left open while the outer door is closed. All Heraeus CO2 incubator glass doors have a safety catch which reliably prevents this from happening. Stable culture conditions are ensured and protection is provided against contamination penetration.

EXTREMELY ECONOMICAL

Standard features

Gain valuable time

HERAcell incubators include a wide range of standard features that optimize the safety of your cultures. Compare HERAcell to other incubators!

Persistent contamination can delay projects. HERAcell incubators require less effort for cleaning and decontamination than competitive models. You will have more time for your research projects or tests.

HERAcell Common CO2 incubators

The fully automatic auto-start routine renders the incubator operational within a few hours. Water-jacket incubators require in excess of a day for start-up because the water requires heating.

MEASUREMENTS

100/3.9

637/25.1

100/3.9

Front view HERAcell (mm/in)

ContraCon decontamination routine works quickly and thoroughly with even the most persistent germs.

766/30.2

Features

✘❏

❏

Automatic decontamination routine

✘❏

❏

It decontaminates the entire interior chamber. There is no time consuming removal and assembling of fittings, hand cleaning of water trays or hours of chemical cleaning, rinsing and drying of sensors.

867/34.1

Interior with rounded corners and stainless

Side view HERAcell (mm/in)

steel fittings

✘❏

❏

Left or right hinged doors

✘❏

❏

Glass door safety catch

✘❏

❏

Upper temperature limit

100/3.9

780/30.7

100/3.9

240

protection for emergency operation

✘❏

❏

RS 232 interface

✘❏

❏

Floating contact

✘❏

❏

Auto-start routine

✘❏

❏

Pull-out shelf

✘❏

❏

Access port

✘❏

❏

Pump for water exchange

✘❏

❏

Stainless steel fan

Front view HERAcell 240 (mm/in)

834/32.8

934/36.8

Side view HERAcell 240 (mm/in) 07

TECHNICAL DATA Type Dimensions External (w x h x d) Internal

(w x h x d)

Total volume Shelves full width (w x d) No. of shelves, standard/maximum Max. load per shelf/ total load Shelves half width (w x d) No. of shelves, standard/maximum Max. load per shelf/ total load

Unit

HERAcell 150 l internal volume

HERAcell 240 240 l internal volume

mm inch mm inch l cu.ft.

637 x 867 x 766 25.1 x 34.1 x 30.2 470 x 607 x 530 18.5 x 23.9 x 20.9 approx. 150 5.3

780 x 934 x 834 30.7 x 36.8 x 32.8 607 x 670 x 583 23.9 x 26.4 x 23.0 approx. 240 8.4

mm inch no. kg

423 x 445 16.7 x 17.5 3/10 10/30

560 x 500 22.0 x 19.7 3/12 10/30

mm inch no. kg

– – – –

260 x 500 10.2 x 19.7 6/16 5/30

stainless steel/solid copper4) stainless steel/solid copper4)

stainless steel/solid copper4) stainless steel/solid copper4)

verified by accredited laboratories 90/9 25 bacteria, fungi, spores (USP 23) mycoplasma

verified by accredited laboratories 90/9 27 bacteria, fungi, spores (USP 23) mycoplasma

Material Interior chamber Shelves, fan impeller ContraCon decontamination routine Decontamination phase, on all surfaces Period (ambient temperature 20 °C) Efficiency spectrum

°C/hrs hrs

Temperature Temperature control range Temperature deviation, time1) /spatial1) Ambient temperature range Recovery time at 37°C2)

°C K °C min

air jacket temperature control TA +3 … 55 ± 0.1/± 0.4 +18 … 33 ≤ 10

air jacket temperature control TA +3 … 55 ± 0.1/± 0.5 +18 … 33 ≤ 10

Humidity Constant humidity3) Fill amount/water quality Recovery time at 95% rH2,3)

%rH l min

≥ 95 max. 3/distilled or autoclaved ≤ 15

≥ 95 max. 4.5/distilled or autoclaved ≤ 20

Gas purity Recovery time, 5% CO22)

vol – % vol – % bar PSI % min

0 … 20 ± 0.1 0.8 … max. 1 12 … 15 min. 99.5, medical quality ≤5

0 … 20 ± 0.1 0.8 … max. 1 12 … 15 min. 99.5, medical quality ≤ 10

Electrical data Rated voltage Rated output (230/120V) Rated frequency

V kW Hz

1/N/PE AC; 230 0.55/0.60 50/60

1/N/PE AC; 230 0.61/0.62 50/60

kg lb

70 165

85 200

CO2 Measure and control range Control accuracy Inlet pressure

Weight (excl. accessories)

2) 30 sec. door open, to 98 % of initial value 3) the relative humidity inside the incubator may increase during

incubation of open culture vessels

240

4) option

TA ambient temperature

Double chamber

1873/73.7

1734/68.2

HERAcell incubators can be stacked directly on top of each other. For stacking HERAcell 240 incubators please use stack adapter Cat. no. 50068677.

240

200/7.9

HERAcell (mm/in)

200/7.9

HERAcell 240 (mm/in) 08

Achtung: hier Seitenende!!!

1) DIN 12 880, part 2

OPTIONS AND ACCESSORIES

HERAtrays

Support frames

HERAtrays are shelves for the convenient transportation of your cultures. Each incubator shelf can be divided into 4 sections with these trays, They work well with a 3 or 6 door screen.

Support frames are available to provide protection against floor contamination. Choose between a height of 200 mm (8 inches) or 780 mm (31 inches). IR-CO2 gas tester The support frames can also The handheld IR-CO2 gas be castor-mounted, allowing easy transport of the HERAcell. tester is equipped with a maintenance-free infra-red cell to monitor the CO2 concentration. Data download and calibration functions are possible by using optional PM-COM software. The IR-CO2 gas tester performs according to GMP/GLP guidelines.

HERAtrays are available in stainless steel and copper.

3 door/6 door screen HERAcell can be equipped with a 3 or 6 door screen. The screen allows access to defined sections of the incubator without disturbing the inner atmosphere. This minimizes recovery times and contamination risks. The 6 door screen (available for HERAcell 240 only) is gas tight.

Half width shelves

Achtung: hier Seitenende!!! wegen Lochung

The half width shelves allow for separation of the incubators’ interior.

ORDER NUMBERS Catalog numbers Standard equipment: air-jacket heating, ContraCon decontamination routine, right hinged door Type

Description

HERAcell (230V, 50/60 Hz) HERAcell (230V, 50/60 Hz) HERAcell (230V, 50/60 Hz)

stainless steel inner chamber solid copper inner chamber twin chamber incubator, stainless steel inner chamber, complete with support frame twin chamber incubator, solid copper inner chamber, complete with support frame stainless steel inner chamber solid copper inner chamber twin chamber incubator, stainless steel inner chamber, complete with support frame twin chamber incubator, solid copper inner chamber, complete with support frame

HERAcell (230V, 50/60 Hz) HERAcell (120V, 50/60 Hz) HERAcell (120V, 50/60 Hz) HERAcell (120V, 50/60 Hz) HERAcell (120V, 50/60 Hz)

09

Cat. no. HERAcell (150 l internal volume)

Cat. no. HERAcell 240 (240 l internal volume)

51013568 51013569

51015690 51015691

50051905

–

50051906 51013668 51013669

– 51016103 51016104

50051907

–

50051908

–

ORDER NUMBERS Options

Description

left three inner doors for segmented access for stainless steel/copper incubator 6 door gas tight screen* six inner doors for segmented access for stainless steel and copper incubator 6 door gas tight screen* left hinged Half width shelves, stainless steel instead of full width shelves Half width shelves, solid copper instead of full width shelves

Cat. no. HERAcell (150 l internal volume)

Cat. no. HERAcell 240 (240 l internal volume)

51900293

51900293

51900295/51900325

–

– – – –

51900387 51900388 51900358 51900357

Cat. no. HERAcell (150 l internal volume)

Cat. no. HERAcell 240 (240 l internal volume)

Door hinged 3 door screen

Accessories

Description

Support frame Support cart Support frame Support cart with drawers and castors Castor set

200 mm/8 in (without castors) 200 mm/8 in (with castors) 780 mm/31 in (without castors)

50051376 50057161 50051436

50065754 50067224 50065753

780 mm/31 in, 3 drawers set with 4 castors for support frames no. 50051376, 50051436, 50065753 and 50065754 150 l unit: to stack up with BB16 240 l unit: to stack up with BB 6220 and B 5060/B 5061 240 l unit: to stack up two HERAcell 240 Shelf tray 1/3 width; 3 pcs. Shelf tray 1/4 width, 4 pcs. Shelf tray 1/2 width for half width shelf, 4 pcs. Shelf tray 1/3 width; 3 pcs. Shelf tray 1/4 width, 4 pcs. Shelf tray 1/2 width for half width shelf, 4 pcs.

50056459

–

50052528 50051938 – – 50051913 – – 50051914 – –

50052528 – 50066094 50068677 50065805 50065807 50065809 50065806 50065808 50065810

incl. 2 support bars

50051909

50065793

incl. 2 support bars

–

50065795

incl. 2 support bars

50051910

50065794

incl. 2 support bars 100–240 V, 50/60 Hz Pack of 5 spare inlet port filters IrDa computer interface and cable (incl. PM-COM Software)

– 50060283 50060287 50060289

50065796 50060283 50060287 50060289

Cat. no. HERAcell (150 l internal volume)

Cat. no. HERAcell 240 (240 l internal volume)

50051912/50052454

–

– – –

50067225 50067226 50067227

Stack adapter

HERAtray, stainless steel HERAtray, stainless steel HERAtray, stainless steel HERAtray, all copper HERAtray, all copper HERAtray, all copper Additional shelf, full-width, stainless steel Additional shelf, half width, stainless steel Additional shelf, full-width, copper Additional shelf, half width, copper IR-CO2 gas tester

Upgrade kit

Description

3 door screen

three inner doors for segmented access

6 door gas tight screen*

for stainless steel/copper incubator six inner doors for segmented access for stainless steel and copper incubator

Shelves, half width, stainless steel Shelves, half width, copper * without inner glass door. Kendro recommendation: apply 6 door gas tight screen only in combination with half width shelves or HERAtrays.

For Ordering or Technical Information

Internet

http://www.kendro.com

Quality Products – Lifetime Care Registered to ISO 9001. Kendro Laboratory Products meet or exceed stringent quality and product safety standards: CE for the European Union, and UL, cUL or CSA standards S00289 Printed in Germany VN 10C 06/01 4t Frotscher for North America. ©2001 Kendro Laboratory Products. All Rights Reserved

TT-HERAcell 3e

Asia Pacific North Kendro Laboratory Products (H.K.) Limited · Hong Kong · Tel. +852 2711-3910 · Fax +852 2711-3858 · [email protected] Asia Pacific South Kendro Laboratory Products · Lane Cove, Sydney · NSW 2066 · Tel. +61 (2) 9936 1540 · Fax +61 (2) 9427 9765 · [email protected] Europe, Middle East, Africa Kendro Laboratory Products International Sales · Hanau · Germany · Tel. +49 (1805) 536 376 · Fax +49 (1805) 112 114 · [email protected] Latin America Kendro Laboratory Products International Sales · Newtown, CT · USA · Tel. +1 (203) 270-2080 · Fax +1 (203) 270-2210 · [email protected] USA, Canada Kendro Laboratory Products · Newtown, CT · USA · Tel. +1 (800) 522-7746 · Fax +1 (203) 270-2166 · [email protected]

NEW

HERAEUS® HERACELL® 240 – CONTAMINATION PREVENTION WITHOUT COMPROMISE The New Upgraded HERAcell® 240 CO2 Incubator Offers Five Unique New Features

Heraeus® HERAcell® by Kendro Laboratory Products is the ideal CO2 incubator providing everything needed to protect and grow your cell and tissue cultures. Now with 3 new options: ● O2 control with auto-cal ● IR CO2 sensor ● Roller Bottle System

Left: Control panel of the new Heraeus® HERAcell® 240 CO2/O2 incubator. Right: Control panel of the new Heraeus® HERAcell® 240 CO2/O2 incubator with Roller Bottle System. HERAcell® 240 (240 l interior volume)

Two New Standard Features ● The basic model now includes the patented, auto-zero self-balancing CO2 control system ensuring long-term stability of CO2 levels, thereby stabilizing pH values in your culture media. ● Each HERAcell® 240 is equipped with a new water-level alarm to remind you to refill the water reservoir before it can run dry. This ensures high humidity levels preventing evaporation of the culture media. The sensor is decontaminated during the 90 °C ContraCon routine.

Quality Products – Lifetime Care

Unique O2 Control with Auto-Cal HERAcell® 240 now offers two optional O2 control ranges. Choose between 1 to 21 % O2 for applications requiring hypoxic conditions or a wide range from 5 to 90 % O2. The maintenance free zirconium sensor is automatically calibrated by the integrated auto-cal system, ensuring long-term stability of O2 concentration. The sensor is decontaminated during the 90 °C ContraCon routine. Unique New IR CO2 Sensor with Auto-Zero With the new HERAcell® 240 you have the choice between the well-established TC or the unique new IR CO2 sensor. Both with auto-zero, ensuring long-term stability and

thereby more constant pH values for good growth conditions. Both sensors remain in the incubator during the 90 °C ContraCon decontamination routine, eliminating the necessity of manual cleaning as with conventional IR sensors, thus saving time and reducing the risk of contamination. Unique New Roller Bottle System HERAcell® 240 can be equipped with up to 4 bottle turning devices for roller bottles between 58 to 186 mm in diameter. The rotational speed of each device is set independently. This unique system remains in the incubator during the 90 °C ContraCon decontamination routine, eliminating time consuming manual cleaning.

TECHNICAL DATA Type Dimensions External (w x h x d) Internal (w x h x d) Total volume ContraCon decontamination routine Decontamination phase Period (ambient temperature 20 °C) Efficiency spectrum

Unit

HERAcell® 240

mm/inch mm/inch l/cu.ft.

780 x 934 x 834/30.7 x 36.8 x 32.8 607 x 670 x 583/23.9 x 26.4 x 23.0 238/8.4 verified by accredited laboratories 90/9 27 bacteria, fungi, spores (USP 23) mycoplasma air jacket temperature control TA1) +3 … 55 ± 0.1/± 0.5 +18 … 33 ≤ 10

°C/hrs hrs

Temperature Temperature control range Temperature deviation, time 2) /spatial2) Ambient temperature range Recovery time at 37°C 3) Humidity Constant humidity 4) Fill amount/water quality Recovery time at 95% rH 3,4) CO2 Measure and control range Control accuracy Inlet pressure

°C K °C min %rH l min vol-% vol-% bar PSI % min kg/lbs

Gas purity Recovery time, 5% CO 23) Weight (excl. accessories)

≥ 95 max. 4.5/distilled or autoclaved ≤ 20 thermal conductivity sensor 0 … 20 ± 0.1 0.8 … max. 1 12 … 15 min. 99.5, medical quality ≤ 10 85/200

HERAcell® 240 CO2/O2 incubator with Roller Bottle System.

1) T = Ambient temperature 2) DIN 12 880, part 2 3) 30 sec. door open, to 98 % of initial value A 4) the relative humidity inside the incubator may increase during incubation of open culture vessels

ORDER NUMBERS Standard equipment: air-jacket heating, ContraCon decontamination routine, auto-zero, water-level alarm, right hinged door Type

Description

HERAcell® 240 (230V, 50/60 Hz) HERAcell® 240 (230V, 50/60 Hz) HERAcell® 240 (120V, 50/60 Hz) HERAcell® 240 (120V, 50/60 Hz)

stainless steel inner chamber solid copper inner chamber stainless steel inner chamber solid copper inner chamber

Catalog No. 51019557 51019558 51019559 51019560

New Options O2 control1), with 6 door gas tight screen and half-width shelves O2 control 2), with 6 door gas tight screen and half-width shelves IR CO2 Sensor Roller Bottle System for stainless steel/solid copper

1) N supply 2

vol-% vol-% 1 Level 2 Levels 3 Levels 4 Levels

1 ... 21 5 ... 90

51900702 51900703 51900571 51900572 51900573 51900574 51900614

2) N supply, range: 5 ... 21 Vol-%; O supply, range: 21 ... 90 Vol-% 2 2

HERAcell® 240 CO2/O2 incubator with 6 door gas tight screen, half-width shelves and copper interior.

Asia Pacific Kendro Laboratory Products (H.K.) Limited · Hong Kong · Tel. +852 2711-3910 · Fax +852 2711-3858 · [email protected] Europe, Middle East, Africa Kendro Laboratory Products International Sales · Hanau · Germany · Tel. +49 (1805) 536 376 · Fax +49 (1805) 112 114 · [email protected] Latin America Kendro Laboratory Products International Sales · Newtown, CT · USA · Tel. +1 (203) 270-2080 · Fax +1 (203) 270-2210 · [email protected] USA, Canada Kendro Laboratory Products · Newtown, CT · USA · Tel. +1 (800) 522-7746 · Fax +1 (203) 270-2166 · [email protected] Internet

http://www.kendro.com

Quality Products – Lifetime Care Registered to ISO 9001. Kendro Laboratory Products meet or exceed stringent quality and product safety standards. ©2002 Kendro Laboratory Products. All Rights Reserved. Printed in Germany 5C 06/02 4t Frotscher S00348

HERAcell240 Flyer 2e

For Ordering or Technical Information

ontraCon Exclusive C ine ination rout decontam TE A IN O ELIM PROVEN T ! A M S MYCOPLA

HERACELL® CO2 INCUBATORS: THE NEW DIMENSION Contamination prevention without compromise

THE IDEAL CO2 INCUBATOR The Heraeus® HERAcell® by Kendro Laboratory Products is the ideal CO2 incubator for cell and tissue cultures, including development of ova or embryos at or near body temperature. It provides everything needed to protect and grow your samples: ● Safe and stable incubation conditions ensure

optimum culture growth. ● ContraCon 90 ºC decontamination routine

decontaminates the entire chamber interior and is proven to eliminate mycoplasma. ● Air jacket maintains temperature uniformity and

quick set-up time. ● Direct humidification offers the fastest humidity

recovery times and provides optimal growth conditions for cultures. ● Available in copper or stainless steel. ● NEW All HERAcell® incubators are also available

with optional Trigas-O2-control for hyperoxic applications. ● 2 convenient sizes: 240 l (8.5 cu.ft.) and

150 l (5.3 cu.ft.). HERAcell® incubators are specifically designed to protect your valuable cultures.

HERAcell® 150 [150 l (5.3 cu.ft.) internal volume] 2

GENERAL FEATURES

ContraCon – effective decontamination A feature exclusive to Heraeus® incubators, ContraCon is an automatic decontamination routine that uses moist heat at 90 ºC. In independent tests, the ContraCon routine was proven to completely eliminate mycoplasma. ContraCon maintains a constant surface temperature on all contamination-prone surfaces. ContraCon’s decontamination efficiency was tested by a GLP approved laboratory and CAMR, Porton Down, UK. Contamination protection Fewer surfaces means less area for contamination. HERAcell® is designed to have fewer contaminationprone surfaces than competitive models.

NEW Humidity system protects cultures from drying out HERAcell® uses a new type of water reservoir – one that replaces the standard water tray. This new system provides a high relative humidity (rH) and most importantly, allows rapid humidity recovery times. Compared to common CO2 incubators with water trays, the HERAcell’s® humidity recovery time is up to five times faster. NEW Exclusive Water-level alarm, 1–2 days before the water reservoir is running dry All instruments are now equipped with a new type of water-level sensor which provides an audible and visual alarm in time when the water reservoir has to be refilled. Good growth for cultures Consistent culture conditions exist throughout the incubator interior. This is achieved by the air jacket temperature control. A newly developed uppertemperature limit protection ensures exact incubation temperature even in the event of a sensor failure. In addition, the TC CO2 sensor ensures long term stability of the incubation atmosphere. NEW IR CO2 sensor All HERAcell® incubators can be equipped with the patented, self-equilibrating IR-measuring system. This is the first IR CO2 sensor designed to resist a 90 °C thermal decontamination procedure (ContraCon).

HERAcell® 240 [240 l (8.4 cu.ft.) internal volume] 3

CONTAMINATION PREVENTION – SAFETY THROUGH DESIGN The reduction of contamination-prone surfaces inside the chamber is the most efficient method of preventing contamination in the long term.

Reduced internal surface areas The consequent reduction of the total surface area and especially minimizing inaccessible corners, cavities, tubing and pipes simplifies cleaning. HERAcell® has up to 2.5 m2 less contaminable surface area than other CO2 incubators. This corresponds to about the area of a door. Without hidden and inaccessible surfaces formation of hidden contamination is greatly reduced.

Completely thought out In contrast to other CO2 incubators, all HERAcell® surfaces are easily accessible. Potential sources of contamination, for example plastic fans and filter casings hidden behind ceiling panels, are not used in the HERAcell®.

Less means more HERAcell® components have up to 50 % fewer contamination-prone surfaces. This means less timeconsuming cleaning and greater contamination protection.

Contamination-prone surfaces [m2] – HERAcell® compared with other CO2 incubators 4.19

3.26 2.95 2.68 2.47

Easy cleaning HERAcell® has a completely smooth inner casing with rounded corners on all sides. The stainless steel interior has an electropolish finish achieving the highest quality of available surface.

1.8

HERAcell® 150

4

Other CO2 incubators

The easy snap in shelf mounting system uses no screws, bolts or other potential contamination traps in the interior space.

Access port HERAcell® incubators are supplied with a 42 mm (1.6 in) access port as standard. This allows cables, plugs and tubing to be easily inserted into the chamber.

High-Low Humidity function For long term incubation HERAcell® offers an additional High-Low Humidity function providing humidity levels of ≥ 95 % rH or ~ 90 % rH.

Convenient operation The pull-out tray underneath the incubator provides space for storage of supplies and operating instructions, as well as a place for research notes. It also serves as a convenient shelf during loading and unloading.

Newly developed water-level alarm This feature provides an audible and visual alarm when the water reservoir needs to be refilled to ensure a constant high level of humidity and to prevent cultures from drying out. The sensor is decontaminated during the 90 °C ContraCon decontamination routine.

Flexible application Doors can be set up for left or right handed usage optimizing your work space. All door gaskets can be removed by hand. They have smooth surfaces for easy cleaning.

Solid copper interior Solid copper is well known to prevent effectively contamination growth on its surface. The HERAcell® is available with a solid copper interior (chamber, complete shelfing system and fan) offering a perfect continuous contamination control.

Novel water reservoir The inner casing sloping down towards the back creates a water reservoir. This avoids inconvenient handling and cleaning of a water basin and eliminates potential contamination area.

6

TECHNOLOGY FOR MORE SAFETY HERAcell® takes care of the safety of your cultures thanks to ■ reliable automated decontamination routine ContraCon ■ relative humidity protection ■ reduction of contaminable surfaces ■ more safety in detail Maximum precautions In order to secure constant incubation conditions, HERAcell® incubators possess a number of important protection mechanisms: Temperature The new upper temperature limit protection not only provides an alarm in case of overheating, but also maintains the specified incubation temperature via a second automatic control loop.

Reliable controls Two large illuminated displays provide instant status of incubator parameters. With a single glance you can check temperature and CO2concentration at the same time – even in passing.

7

108

64

38 29

HERAcell® 150

Safety in detail All Heraeus® CO2 incubator glass doors have a safety catch which reliably prevents the inner door from mistakenly being left open. Stable culture conditions are ensured and protection is provided against contamination penetration.

HERAcell® 150 incubators ensure consistency of the most important incubation parameters and effectively protect valuable cell cultures.

ContraCon’s effectiveness on all surfaces was tested by a GLP approved laboratory and CAMR, Porton Down, UK. A second independent laboratory tested and confirmed ContraCon’s complete elimination of mycoplasma.

120

60

Humidity The novel water-level alarm continuously monitors the water level and provides an early warning if the water reservoir has to be refilled. Gassing The optional gas guard for CO2 and O2/N2 permits 2 respective gas supplies to be connected. As soon as the first supply is empty the controller switches automatically to the second supply and sounds an alarm.

Humidity recovery times [min] – HERAcell® compared with common CO2 incubators

Contaminants tested: ● Bacillus subtilis ● Bacillus stearothermophilus (USP 23) ● Enterococcus faecalis ● Escherichia coli ● Pseudomonas aeruginosa ● Staphylococcus epidermidis ● Corynebacterium xerosis ● Aspergillus niger

Other CO2 incubators

Reliable decontamination with ContraCon The ContraCon automatic decontamination routine consistently achieves excellent decontamination at a moist heat of 90 ºC. In less than a day, the HERAcell’s® entire interior, including all sensors, shelves, fan and the critical water reservoir, are decontaminated. You save time by not having to remove, refit or separately decontaminate any fixtures. There is no limit to the number of times that ContraCon can be used for decontamination. A homogenous heat distribution on all surfaces is important for effective decontamination.

Protected from drying out Especially when the door is opened frequently or when low media volumes are used, short recovery times become very important. The HERAcell® incubators inclined/rounded cornered water reservoir provides a large water surface and a direct heat transfer from the heated floor to the humidification water. With water tray incubators, a layer of air between the incubator and tray, slows down the humidity recovery, resulting in high culture media evaporation rates. Together with the patented, additional floor heating after door opening and during O2 gassing, the HERAcell® achieves humidity recovery times up to five times faster than competitive models.

Gas tight inner glas door All HERAcell® incubators now offer an optional 3 door (HERAcell® 150) or 6 door ( (HERAcell® 240) inner glas door. Inner doors allow access to defined sections of the incubator without disturbing the inner atmosphere. This minimizes recovery times and contamination risks.

EXTREMELY ECONOMICAL

Standard features HERAcell® incubators include a wide range of standard features that optimize the safety of your cultures. Gain valuable time Persistent contamination can delay projects. HERAcell® incubators require less effort for cleaning and decontamination than competitive models. You will have more time for your research projects or tests. There are considerably fewer surfaces to clean with the new HERAcell® instruments. Furthermore, “normal cleaning“ is sufficient, as decontamination is done automatically in the incubator – autoclaving is no longer necessary. The fully automatic auto-start routine renders the incubator operational within a few hours. Water-jacket incubators require in excess of a day for start-up because the water requires heating.

ContraCon decontamination routine works quickly and thoroughly with even the most persistent germs. It decontaminates the entire interior chamber. There is no time consuming removal and assembling of fittings, hand cleaning of water trays or hours of chemical cleaning, rinsing and drying of sensors. The reliable safety of the HERAcell® incubators is also an economic factor: fewer problems and breakdowns mean an increase in the efficiency of your work.

HERAcell® Other CO2 incubators Basic features

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Automatic decontamination routine

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All sensors thermally decontaminable

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Interior with rounded corners and stainless steel fittings

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Reduced interior surfaces

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All fittings made of stainless steel, incl. fan

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Left or right hinged doors

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Glass door safety catch

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Upper temperature limit protection

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RS232 interface

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Floating alarm contact

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High/Low Humidity function

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Water-level sensor

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Access port

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Pull-out shelf

Available Options

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Solid copper interior

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Gas tight inner glass door

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O2 control

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Integrated gas guard

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