DM6000 FS Modular System Guide Jan 2012

Leica DM6000 FS Base Stand The Leica DM6000 FS is a research system microscope with motorized and automated functions for electrophysiological applications. The following contrast methods come into use: • Bright field • Phase contrast • Simple polarization contrast • Differential interference contrast (DIC) • Fluorescence The features: Adjustments of diaphragms and filters as well as adjustments of luminous intensity to the magnification and contrast methods can be carried out and reproduced automatically. The fluorescence illuminator, including filters, diaphragms and shutters, is completely automated. All motorized functions are controlled and displayed using the Leica STP6000 touch panel.

Fig. 1 Leica DM6000 FS

142 mm

142 mm

112 mm

391 mm 248 mm

385 mm 355 mm

42.5 mm

Fig. 2 Footprint

4

147 mm 185 mm

Fig. 3 Rear side

147 mm 185 mm

The Leica DM6000 FS Microscope Stand is composed of the following equipment, together having one order number:

Base stand 11 888 928 Consisting of: Stand base • Transmitted-light diaphragm module with automatic Constant Color Intensity Control (CCIC), infrared filters 750 – 850 nm and shutter. • Sealed transmitte-light output. • Baseplate with manual swing diffuser, which is switched off when the Dodt contrast method is activated. • Illuminator holder: Leica standard interface for transmittedlight lamp housings. • 4 mounting holes for fastening to anti-vibration tables or manual or motorized X-Y translation stages for moving the complete microscope. Rear side of the stand With various electronic interfaces • 1 x CTRMIC (external electronics housing) • 1 x RS232C • 2 x USB2 • 4 x EXT (for external auxiliary equipment)

Fig. 4 Leica DM6000 FS with Leica STP6000

Focus Motorized objective focusing with focus guide box and guide plate for changeable objective holders • Motorized travel range: 12 mm • Smallest translation: 0.015 μm • Max. speed: 5 mm/sec Guide plate • Encoded guide plate for three different objective holders and nosepieces with integrated fixture for DIC - objective prism slide possible Upper part of stand Consisting of: Receptacle for analyzer slider

Fig. 5 Leica DM6000 FS, rear view of the stand

1" motorized fluorescence axis • Incident-light diaphragm module with motorized FIM (fluorescence intensity manager) and motorized field diaphragm disk • 5-position encoded and motorized fluorescence cube disk • Illuminator holder: Leica standard interface for fluorescence lamp housing or 1" light guide couplers (such as the Leica EL6000) Tube change interface • For holding the fixed stage tube or Leica standard tubes 2 slide holders for filters or boosters 5

Control Boxes and Trigger Boxes Leica CTR5500 electronics box For use with a manual or fixed stage. With built-in power supply 12 V 100 W and control of the automatic microscope functions. Includes USB cable. AC power supply 90–250 V, 50–60 Hz 11 888 820

Fig. 6 Leica CTR7000 HS

Leica CTR6000 electronics box For use with a motorized (rack & pinion) stage. With built-in power supply 12 V 100 W for controlling the automatic microscope functions. Includes USB cable. AC power supply 90–250 V, 50–60 Hz 11 888 821 Leica CTR6500 electronics box For use with a motorized (spindle) scanning stage. With built-in power supply 12 V 100 W and for control of the automatic microscope functions. Includes USB cable. AC power supply 90–250 V, 50–60 Hz 11 888 822

Fig. 7 Leica CTR7000 HS, rear side

Leica CTR6500 HS electronics box For use with a motorized (spindle) scanning stage. With built-in power supply for 12 V 100 W and for controlling the automatic microscope functions and built-in sequencer for controlling the simple trigger box (11 640 004). Includes USB cable. AC power supply 90–250 V, 50–60 Hz 11 640 010 Leica CTR7000 HS electronics box (page 6, Fig. 6) For use with a motorized (spindle) scanning stage. With built-in power supply for 12 V 100 W and for controlling the automatic microscope functions and built-in sequencer for controlling the multi-trigger box and/or the NiDAQ board (11524007). Includes USB cable. AC power supply 90–250 V, 50–60 Hz 11 640 014

6

Leica SmartMove (page 7, Fig. 8) XYZ ergo control panel (without display) for controlling the electronic focus (z) and the motorized stage (xy). With 4 freely programmable function keys. 11 505 180 Leica STP6000 Smart Touch Panel (page 7, Fig. 9) XYZ ergo control panel with integrated color-touch screen / display and 11 variable function buttons for controlling every microscope function (focus, stage, DIC, objectives and fluorescence. 11 501 255 Peripheral triggering adapter(page 7, Fig. 10) A simple trigger box for connecting up to three external devices, such as a camera trigger signal, or system trigger "IN" (as start trigger for a sequence) and system trigger "OUT" (for synchronization). 11 640 004

Fig. 8 Leica SmartMove

BNC box (trigger box) (page 7, Fig. 11) Trigger for 5 system triggers (2 IN / 3 OUT), trigger for IL or TL shutter, synchronization trigger for NiDAQ box and camera trigger as well 11 640 002 NiDAQ box (page 7, Fig. 12) Data acquisition box for connecting up to 16 analog data sources with 16-bit resolution and up to 200 Hz sampling frequency 11 524 007

Fig. 9 Leica STP6000

Trigger demo set For testing and demonstration purposes, a trigger set is offered that can simulate one or more triggers via an external switch. The set consists of two cables and one pushbutton switch. 11 102 279

Fig. 10 Peripheral triggering adapter

Fig. 12 NiDAQ box

Fig. 11 BNC box (trigger box)

7

PC and Software

Fig. 13 Leica DM6000 FS

Leica AF6000 E without monitors The Leica AF6000 E includes a software license and a PC with mouse and hardware dongle. In addition to comprehensive "Online Help", the software license covers the following modules: "2D Time-lapse", "Acquisition", "Image Overlay", "Sharpening", "Blurring", "Micron Marker", "Single Line Measurement", and "Change of Contrast, Brightness, Gamma". "Z-stacking" and support for motorized stages are not included in this license. Keyboard and monitor also need to be ordered separately. 11 640 016 For detailed description of the modules and licenses, refer to the AF6000 E product documentation. Leica AF6000 without monitors The Leica AF6000 includes all of the components of the AF6000 E. In addition, the Leica AF6000 is designed for fast data acquisition and large data records, and triggering electrophysiological data and images. Other applications include, among others: "Multichannel" with fast image-overlay, "Z-stacking","Mark and find", and "Tile-scan". The Leica AF6000 includes a software license, a PC with mouse, and a hardware dongle. Keyboard and monitor need to be ordered separately. 11 640 008 For detailed description of the modules and licenses, refer to the Leica AF6000 E - 7000 product documentation. Live Data Mode Leica Application Suite Live Data Mode. Definition of data acquisition in "jobs" and "macros". It is possible to modify the data acquisition during the measurement. Trigger functions are implemented. 11 640 803 LCD monitor 22" (1680x1050) The Leica AF6000 E supports either one monitor or two of the same type. 11 600 261 Flat-screen monitor TFT 19" The Leica AF6000 E supports either one monitor or two of the same type. 11 600 169 USB PC keyboard French English (US) English (UK) Swedish Italian German Spanish

11 600 219 11 600 220 11 600 221 11 600 222 11 600 223 11 600 224 11 600 225

For cameras, refer to "Digital Image Documentation" in this brochure. 8

System Designs Three system designs are available. • Basic version without sequencer • System version with sequencer based on CTR6500 HS • High-end version with sequencer based on CTR7000 HS and data acquisition box

Basic version without sequencer • Leica DM6000 FS • Leica CTR5500 for manual stage or • Leica CTR6000 for motorized stage or • Leica CTR6500 for scanning stage • Leica STP6000 control panel • Leica AF6000 E • Camera

Fig. 14 Leica DM6000 FS basic version without sequencer

CTR-MIC-Connection

USB USB

Leica CTR5500 Leica CTR6000 Leica CTR6500

Firewire Camera Leica DFC365 FX

Leica DM6000 FS

Leica STP6000

PC with Leica AF6000 E

1-2 Monitors

9

System version with sequencer based on CTR6500 HS • Leica DM6000 FS • Leica CTR6500 HS • Small trigger box (peripheral triggering adapter) Camera trigger One system trigger IN (e.g. start-trigger for the sequence) One system trigger OUT (e.g. for synchronizing external instruments during sequence start) • Leica STP6000 • Leica AF6000 • Live Data Mode • Camera

Fig. 15 Leica DM6000 FS system version with sequencer

Trigger Box (small)

CTR-MIC-Connection

USB Leica CTR6500 HS

Out Trigger

External Device

In Trigger

External Device

Camera Trigger

USB

Firewire Camera Leica DFC365 FX

Leica DM6000 FS

Leica STP6000

10

PC with Leica AF6000 + Live Data Mode

1-2 Monitors

High-end version with sequencer based on CTR7000 HS and data acquisition box • Leica DM6000 FS • Leica CTR7000 HS • BNC box (trigger box) Camera trigger One system trigger IN (e.g. start-trigger for the sequence) One system trigger OUT (e.g. for synchronizing external instruments during sequence start) Two synchronization triggers for the data acquisition box (start-trigger and synchronization clock for image and data) • Data acquisition box Up to 16 analog channels via the data acquisition box • Leica STP6000 • Leica AF6000 • Live Data Mode • Camera

Fig. 16 Leica DM6000 FS high-end version with sequencer

Trigger Box (large)

CTR-MIC-Connection

USB Leica CTR7000 HS

Camera Trigger

Out Trigger

External Device

In Trigger

External Device

Start Trigger Sync. Trigger

USB

Firewire

Data Acquisition Box (NiDAQ)

Camera Leica DFC365 FX

Leica DM6000 FS

USB 1...16 Analog Inputs External Analog Device

Leica STP6000

PC with Leica AF6000 + Live Data Mode

1-2 Monitors

11

Objective Holders, Objective Changers and Objective Nosepieces The upper part of stand of the Leica DM6000 FS is equipped with an encoded objective receptacle for three different objective holders, objective changers or objective nosepieces each with an integrated holder for a DIC – objective prism slide. The objective holders, changers or nosepieces can be switched without tools and are optionally interchangeable. The microscope's electronics identify and activate them. With encoding, the system recognizes the objective in the beam path and automatically sets the obtained luminous intensity, the aperture and field diaphragm settings, as well as the desired contrast method. Every objective holder, changer or nosepiece is controlled by the motorized objective focus. As such, the automatic functions made available through the Leica Application Suite (LAS) can be used for all objectives, such as the "DIP-IN" function, which allows the objective to be lowered from the focus position into the fluid medium, and then, after the front lens has been wetted, returned back to focus-all without vibration and without disturbing the experimental setup. Objective holder for single objective (page 12, Fig. 17) An encoded individual slide bar for holding a fixed single objective with 75 mm parfocalizing distance and M32 x 0.75 mm objective threading. Including holder for a DIC objective-prism slider. With the fixed objective, 360° of work surface around the objective are available for microtools. 11 555 089 When using an objective with 45 mm parfocalizing distance and M25 x 0.75 mm objective threading, the parfocal adapter 45/75 is additionally required. 11 505 259 Fig. 17 Objective holder for single objective

12

Motorized objective changer for 2 objectives (Page 13, Fig. 18) An encoded and motorized 2-position objective changer for vibration-free objective changing, for example two water immersion objectives with M25 x 0.75 mm objective threading and 45 mm parfocalizing distance. Changing takes place with precise focusing including liquid wetting; no readjustment is necessary. Including holder for a DIC objective-prism slider. Because none of the objectives swings out toward the side or front, a working area of over 320° is kept free for the use of microtools. To prevent vibration and bubble formation, the objectives are moved in and out of the immersion at an angle and swung away upward and back on an elliptical track, while the second objective carries out the converse motion. 11 524 006 Motorized objective nosepiece for up to 6 objectives (page 13, Fig. 19) An encoded and motorized 6-position objective nosepiece for vibration-free objective changing, for example multiple water immersion objectives with M25 x 0.75 mm objective threading and 45 mm parfocalizing distance. Including holder for a DIC objective-prism slider. The nosepiece is rotated 90° laterally and is installed at a 22.5° angle to ensure that the objective is swung out almost precisely straight, either towards the front or back. When only three objectives are used, the trajectory of their swinging in and out is practically straight. To prevent vibration and bubble formation, the objectives are moved in and out of the immersion at an angle. 11 501 249

Fig. 18 Motorized objective changer for 2 objectives

Fig. 19 Motorized objective nosepiece for up to 6 objectives

13

Tubes, Magnification Changers and TV Adapters For the Leica DM6000 FS, a motorized trinocular tube that is specially designed for infrared applications is offered with a tube lens system that is adapted to fit the magnification changer for the Leica DM6000 FS. When no magnification changer is being used on the microscope, any manual tube belonging to the Leica DM series can be used. The tubes are securely attached to the stand cover with positive locking using precise dovetail ring switching and clamping screws. The eyepiece tubes of the tube system are designed based on the Siedentopf principle. Trinocular Tube DM FS(page 14, Fig. 20) Motorized, binocular observation tube for fixed stage with photo output for 2 magnification changers and/or one TV adapter, with 2 switch positions for 100% light to the eyepiece or 100% light to the camera output. 11 524 005

Fig. 20 Trinocular Tube DM FS with 3-position magnification changer

3-position magnification changer (page 14, Fig. 20) For Leica DM6000 FS (0.35x - 1.25x - 4.0x) Motorized, 3-position magnification changer including C-mount threading for the binocular observation tube for fixed stage with photo output (11 524 005) for use with fixed single objectives (e.g. 25x/ 0.95) with optical magnification levels 0.35x, 1.25x and 4.0 x. 11 524 002 2-position magnification changer For Leica DM6000 FS (1.25x - 4.0x) Motorized, 2-position magnification changer including C-mount threading for the binocular observation tube for fixed stage with photo output (11 524 005). For use, for example, with the motorized objective changer for 2 objectives (11 524 006) equipped with a 5x or 10x scanning objective and a working objective, e.g. 25x/0.95 W, with optical magnification levels 1.25x and 4.0 x. 11524003

Fig. 21 Trinocular Tube DM FS with 1-position camera adapter

14

1-position camera adapter (page 14, Fig. 21) For Leica DM6000 FS (1.25x) 1-position fixed magnification lens, C-mount threading for the binocular observation tube for fixed stage with photo output (11 524 005). For use, for example, with the motorized objective nosepiece for 6 objectives (11 501 249) equipped with a 5x scanning objective and/or 10x, 20x, 40x, 63x UVI water immersion objectives. 11 524 004

Fixed tubes of the DM series Tube BT25+ (page 15, Fig. 22) Binocular observation tube, fixed 30° viewing angle.

11 505 147 Tube BDT25+ (page 15, Fig. 23) Binocular observation tube with photo port, fixed 30° viewing angle, 3 switch positions: Beam split: 100%:0%, 50%:50%, 0%:100% 11 505 146

Fig. 22 Tube BT25+

Tube BDTP25 Like the BDT25+, but with orientation of the right eyepiece so that the cross-hairs remain aligned in the Pol eyepiece during adjustment of the interpupillary distance. 11 551 076

Photo holder sockets For 11501249 or tube BDTP25

Fig. 23 Tube BDT25+

Mono tube top attachment Photo/TV holder socket with one output.

11 505 161

Alternatively: Dual tube top attachment, fixed (50:50) Photo/TV holder socket with two outputs.

11 505 162

Alternatively: Dual tube top attachment, selectable (100:100), manual Photo/TV holder socket with two outputs. 11 505 223

Fig. 24 Tube AET22

Ergonomic tubes: Tube AET22 (page 15, Fig. 24) Binocular ergonomic tube with variable viewing angle 5°–32° and variable eyepiece extension 0–30 mm. 11 505 148 Tube EDT22 (page 15, Fig. 25) Binocular observation tube with photo port with variable viewing angle 5°–32°. Fixed beam splitting 50%:50%. 11 505 149

Fig. 25 Tube EDT22

Tube with image correction Tube HC L 2TU 4/5/7 (page 15, Fig. 26) Binocular observation tube with photo port with image correction, 20° viewing angle, beam split 100%:100%. 11 501 598

Fig. 26 Tube HC L 2TU 4/5/7

15

TV adapter (page 16, Fig. 27/28)

Fig. 27 TV adapter

You can adapt analog and digital cameras to all tubes with documentation output. The C-B and F-mount adapters are aligned to the dimensions of the holder thread. The various fixed and variable magnification factors allow adjustment of the rendering of the microscopic image on the camera chip. In order to display the largest possible portion of the field of view on the monitor, the magnification factor of the adapter must fit the chip size of the camera. If the magnification is too low, there will be a lack of uniformity to the illuminated area (shading) and/or vignetting.

Fig. 28 Vario TV adapter

Recorded picture diagonal in mm with 1-inch camera

2/3-inch camera

1/2-inch camera

1/3-inch camera

Order No.

Without zoom magnification, for 1-chip cameras only: C-mount adapter 1x HC

16

11

8

6

11 541 510

C-mount adapter 0.7x HC

–

15.7

11.4

7.8

11 541 543

C-mount adapter 0.55x HC

–

–

14.5

10.9

11 541 544

C-mount adapter 0.35x HC

–

–

–

17.1

11 541 512

–

19*–5

18–3.8

11 541 517

With variable magnification level (Vario TV adapter) for 1–3 chip cameras: C-mount 0.32–1.6x HC

–

Without variable magnification level, for 1-3 chip cameras: C-mount adapter 1x

–

–

16

12

11 543 706

B-mount adapter 1x

–

–

16

12

11 543 702

Additionally required for each: TV optics 0.5x HC * available beginning with Vario factor 0.42 x!

16

11 541 545

Stages and Specimen Holders Specimen stages on upright microscopes are attached to a stage bracket. On the Leica DM6000 FS, this stage bracket is located on the condenser holder and is designed for "demonstration stages" and smaller stages only. This is because decoupled stages are used with microscopes designed for electrophysiological applications, meaning the stages are not connected to the microscope directly, but instead are part of a working platform around the microscope. In this case, instead of the specimen being moved, generally the entire microscope, including the optics, is moved over the specimen. Refer also to: Peripherals for Fixed Stage Applications

Fig. 29 Mechanical stage for DM6000 FS

Four clamping screws are used to adapt the simple specimen stage. This also allows you to adjust the stage height mechanically independent of the drive movement. On mechanical stages, the x-y stage motion is controlled by a closed cable drive without a rack. Mechanical stage for Leica DM6000 FS (page 17, Fig. 29) Cross-stage with ceramic-coated stage plate 188 x 153 mm without rotation, mid-position opening 82 x 30 mm front, adjustment range 76 x 50 mm, right-hand operation with telescoping double connecting rod with separate torque setting for x and y movement. Without slide holder. 11 501 252

Fig. 30 Plug-in slide holder for 2 specimen slides

Ergonomic slide holder for one specimen With spring clips for clamping the front side of the specimen, for simultaneous right-handed stage and focus adjustment. Screw fitting on mechanical stage for DM6000 FS. 11 505 163 Plug-in slide holder for 2 specimen slides (page 17, Fig. 30) For insertion of up to two specimens into a slide adapter. Screw fitting on mechanical stage for DM6000 FS. 11 505 157 Single-hand slide holder for 1 specimen slide (page 17, Fig. 31) For insertion of one specimen into a slide adapter. Screw fitting on mechanical stage for Leica DM6000 FS. 11 505 156

Fig. 31 Single-hand slide holder for 1 specimen slide

Motorized cross-stage for Leica DM6000 FS Motorized cross-stage with step motor with tabletop 234 x 157 mm. Adjustment range 76 x 50 mm. Operation with Leica SmartMove, Leica STP6000 or Leica software. 11 501250 Single-hand slide holder for 1 specimen (page 17, Fig. 32) For insertion of one specimen in a slide adapter. Screw fitting on motorized stage for DM6000 FS. 11 505 181 Plug-in slide holder for 2 specimen slides For insertion of up to two specimens in a slide adapter. Screw fitting on mechanical stage for Leica DM6000 FS. 11 505 182

Fig. 32 Single-hand slide holder for 1 specimen

17

Objectives Based on the Leica principle of infinity distance correction of optics, the microscope objectives are infinity corrected for tube lens systems with 200 mm reference focal lengths. For regular bright field objectives, the parfocalizing distance is 45 mm and the objective threading is M25x0.75 mm. For the fixed stage objective 20x/1.00 W, the optical parfocalizing distance is 75 mm and the objective threading is M32x0.75 mm. For electrophysiology or patch-clamp applications, Leica offers special objective designs.

Fig. 33 Electrophysiological objectives

UVI water immersion objectives Very narrow, special objectives for the demands of electrophysiological applications, with high numerical aperture (N.A.), large free working distance (FWD), large access angle (Acc<) and precise, objective-side Wollaston prism, apochromatically corrected with flat front lens and a front area of Marcor and Macrolon. This eliminates the risk of damage to the optics and achieves a very high objective wettability. The following 4 objectives with 45 mm parfocalizing distance and M25x0.75 mm objective threading available: Objective

N.A.

FWD

Acc<

DIC

Prism S1/0.9

Order number

HCX APO L 10x / 0.30 W U-V-I

0.30

3.7 mm

> 45°

D1

K2

11 506 142

HCX APO L 20x / 0.50 W U-V-I

0.50

3.6 mm

> 45°

D1

K3

11 506 147

HCX APO L 40x / 0.80 W U-V-I

0.80

3.3 mm

> 45°

D1

K6

11 506 155

HCX APO L 63x / 0.90 W U-V-I

0.90

2.2 mm

> 43°

D1

K7

11 506 148

High-aperture infrared-optimized 25x electrophysiology objective (page 18, Fig. 34) Compact, special objective for the demands of electrophysiology applications. With optimized correction in the near infrared range (up to 900 nm), with high numerical aperture (N.A.), large free working distance (FWD), large access angle (Acc<). Apochromatically corrected with flat front lens and a front area of Marcor and Macrolon. This eliminates the risk of damage to the optics and achieves a very high objective wettability. The parfocalizing distance is 45 mm and the objective threading is M25x0.75 mm.

Fig. 34 Objective HCX IRAPO L 25x/0.95 W

Objective

N.A.

FWD

Acc<

DIC

Prism S1/0.9

Order number

HCX IRAPO L 25x/0.95 W

0.95

2.5 mm

> 41°

H2

K5

11 506 323

! When using this objective with the objective holder for single objective (11 555 089), the parfocal adapter 45/75 (11 505 259) is additionally required.

18

With the 3-position magnification changer (0.35x - 1.25x - 4.0x) 11 524 002, the following specifications, among others, result for the objective. Magnification changer

N.A.

Field of view in mm

Resolution in µm

Depth of focus in µm

Equivalent objective* 12.5x/0.25

0.35

0.25

1.28

1.10

11.6

1.25

0.95

0.36

0.29

0.81

40x/0.95

4.00

0.95

0.11

0.29

0.81

150x/0.95

High-aperture confocal-optimized 20x electrophysiology objective (page 19, Fig. 35) Compact special objective for the demands of electrophysiology applications, optimized for confocal and 2-photon applications, with high numerical aperture (N.A.), large free working distance (FWD), large access angle (Acc<). Apochromatically corrected with flat front lens and a front area of Marcor and Macrolon. This eliminates the risk of damage to the optics and achieves a very high objective wettability. The parfocalizing distance is 75 mm and the objective threading is M32x0.75 mm.

Fig. 35 Objective HCX APO L 20x/1.0 W

Objective

N.A.

FWD

Acc<

DIC

Prism S1/0.9

Order number

HCX APO L 20x/1.0 W

1.00

2.0 mm

> 39°

H/H2

K7/K16

11 507 701

! The objective holder for single objective 11 555 089 (page 19, Fig. 36) is always required for this objective. With the 3-position magnification changer (0.35x - 1.25x - 4.0x) 11 524 002, the following specifications, among others, result for the objective. Magnification changer

N.A.

Field of view in mm

Resolution in µm

Depth of focus in µm

Equivalent objective*

0.35

0.25

1.60

1.10

11.6

10x/0.25

1.25

1.00

0.45

0.27

0.73

32x/1.0

4.00

1.00

0.15

0.27

0.73

110x/1.0

For a detailed objective list, refer to http://www.leica-microsystems.com/objectives There you can also find all other bright field objectives of the various objective classes. Even earlier-type objectives can be adapted for further use. There is an adapter for objectives with RMS thread. When selecting the objectives, consider the intended use with regard to specimen covering, etc. For additional information on using objectives with various condenser heads and/or various contrast methods, refer also to the chapter on Contrast Methods in this brochure.

Fig. 36 Single objective holder

*) Equivalent objective: (11.2 mm camera chip / field of view) / 0.7 C-mount

19

Eyepieces and Graticules Eyepieces for tube eyepiece

0 1 2 3 4 5 6 7 8 9 10

Fig. 37 Graticule 11 506 950

All eyepieces have removable or fold-down eyecups and can be used with or without eyeglasses. Oculars identified with M are equipped with a focusing eyelens for dioptric equalization (from –6.8 to +4.2 or –6 to +5) and graticule holder. The outer diameter of the eyepiece is D = 30 mm. Graticule diameter D = 26 mm. The eyepiece data are engraved, e.g. HC PLAN 10x/20 $ M. HC PLAN = correction type, 10x = magnification/20 = field number FOV, $ = glasses type (high exit pupil), M = dioptric adjustment/graticule holder.

11506950

Fig. 38 Graticule 11 506 951

11506951

Fig. 39 Graticule 11 506 952

11506952

Eyepieces with FOV 20 • Eyepiece HC PLAN 10x/20 BR. • Eyepiece HC PLAN 10x/20 BR.M

11 507 801 11 507 802

Eyepiece with FOV 22 • Eyepiece HC PLAN S 10x/22 BR.M

11 507 807

Eyepiece with FOV 25 (Fig. 118 p. 45) • Eyepiece HC PLAN S 10x/25 BR.M

11 507 808

Special eyepieces with high magnification • Eyepiece HC PLAN 12.5x/16 BR.M • Eyepiece 16x/14B, adjustable • Eyepiece 25x/9.5B, adjustable • Spacer ring for eyepiece 16x/14B and eyepiece 25x/9.5B

11 506 515 10 445 301 10 445 302 11 506 808

Graticules for length measurements, comparison and counting methods For HC PLAN eyepieces • Graticule 10 mm = 100 divisions, D = 26mm • Graticule 10 mm = 200 divisions, D = 26mm • Crosshair graticule, D = 26 mm • Crosshair graticule with graduation, 10 mm = 100 divisions, D = 26 mm • Crosshair graticule with grid 10 x 10 mm, 0.1 mm graduation, D = 26 mm • Crosshair graticule with grid 10 x 10 mm, 1 mm graduation, D = 26 mm

11 506 950 11 506 951 11 506 953 11 506 952 11 506 954 11 506 955

Fig. 40 Graticule 11 506 953 11506953

20

Fig. 41 Graticule 11 506 954

Fig. 42 Graticule 11 506 955

Transmitted-Light Contrast Method, Condensers The transmitted-light illumination system is based on a commercially available 12 V, 100 W halogen bulb, the luminous intensity of which is automatically adjusted to the preset value based on the density of the specimen and the light stream in the objective. The adjustment is color-neutral, i.e. the color temperature remains constant. Refer to the corresponding chapters in this brochure for information on the various light sources, lamp housing and corresponding power supplies. The different transmitted-light methods generally require different condensers and/or condenser heads. The Leica DM6000 FS uses a condenser that is specially designed for applications in electrophysiology. Here it is important that the seal between the condenser head and the housing is watertight and that liquid is led away in the event of a "water accident".

Bright field, phase contrast, dark field, polarizationcontrast and differential interference contrast The encoded objective nosepiece recognizes the objective currently being used, and automatically adjusts the luminous intensity and field diaphragm to preset values. The user can adjust and overwrite the preset values at any time. Required components: Leica DM6000 FS Condenser (page 21, Fig. 43) Centerable condenser for Koehler illumination for objectives with magnifications 10x–100x for bright field, phase contrast, polarization contrast and differential interference contrast. Complete with: 11 505 245 • Manual condenser holder for attaching to the stand column and a positioning range of 28 mm and a manual stop for the optimum "Koehler" position. • Sub-condenser with 4 receptacles for prisms or light rings and one receptacle for the condenser head, with manual aperture diaphragm and manual centering for the illuminated field diaphragm (without prisms or condenser head) • Polarizer holder (without polarizer)

Fig. 43 Leica DM6000 FS Condenser

Condenser head 0.90 S1 (page 21, Fig. 44) Focal intercept 1 mm, low strain for all transmitted-light contrast methods. 11 505 150 Condenser head P 0.90 S1 (page 21, Fig. 44) Focal intercept 1 mm, extremely low strain for all transmittedlight contrast methods. 11 551 072 Condenser head P 1.40 OIL S1, achromatic (page 21, Fig. 44) Focal intercept 1 mm, extremely low strain, for highest microscopic resolution or for use with Dodt contrast. 11 551 004

Fig. 44 Condenser heads

21

Bright field For bright field applications, no additional inserts are necessary in the condenser. Contrasting is regulated completely by the aperture and illuminated field diaphragm.

Phase contrast and dark field

Fig. 45 Light rings

The Leica DM6000 FS, like all microscopes of the DM series, can also be equipped for dark field and phase contrast. This requires no more than installing the corresponding light rings or dark field stop in the condenser. These contrast methods, however, are not placed in the foreground when it comes to electrophysiology applications. Dark field is possible with a maximum objective aperture of 0.7. The light rings (page 22, Fig. 45) and the dark field stop, in a 32 mm version, must be inserted into the condenser disk. The following light rings are available on request: Light ring 1 for PH1 phase objectives Light ring 2 for PH2 phase objectives Light ring 3 for PH3 phase objectives Dark field stop

11 023 121 050 008 11 023 123 052 005 11 090 132 050 020 11 090 132 050 026

Focusing telescope (page 22, Fig. 46) For equal coverage adjustment of light and phase ring, and control of the compensation strip for interference contrast. 11 505 070

Fig. 46 Focusing telescope

22