HM 525 Instruction Manual July 2004

HM 525

INTENDED USE

Dear Customer, Before putting the instrument into operation, please read these operating instructions carefully to familiarize yourself with its proper operation and functions. The MICROM cryostat HM 525 is a highly efficient cryostat for sectioning techniques in routine and research. Only skilled or specially trained personnel must operate the microtome cryostat, i.e. placing the specimen onto a chuck, sectioning and transferring sections onto a slide. The listed and marked safety measures as well as the regulations and hygiene measures of your respective lab must strictly be observed.

MICROM Ser. No. :... Please check the MICROM Ser. No. on the type plate, which is placed on the rear side of your instrument and enter this number here. This way, questions and service can be handled faster.

Instruction Manual No. 387779 Issued on July 13, 2004

MICROM International GmbH Robert-Bosch-Strasse 49 D-69190 Walldorf

Telefon: (06227) 836-0 Telefax: (06227) 836-111

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

HM 525

Intended Use Table of Contents EC Certificate of Conformity Safety Precautions

PART 1 INTRODUCTION 1-1

Description of the HM 525

1-2

Technical data HM 525

PART 2 OPERATING INSTRUCTIONS 2-1

Setting up the cryostat

2-2

Initial turn-on

2-3 2-3-1 2-3-2 2-3-3 2-3-4 2-3-5 2-3-6 2-3-8 2-3-9

Basic operational rules Actual and set value of the chamber temperature Defrosting the evaporator Interrupting a defrosting cycle Emptying the defrosting liquid Illumination of the cryo chamber Freezing on a specimen Specimen orientation with zero positioning Handwheel lock

2-4 2-4-1 2-4-2

Cutting process Cutting movement and retraction Setting section thickness and trimming thickness

2-5 2-5-1 2-5-2 2-5-3 2-5-4

Feed Specimen coarse feed Memory function Trimming and first cuts Fine feed

2-6 2-6-1 2-6-2 2-6-3 2-6-4

Indication of cutting processes Section counter Sum of section thicknesses Remaining travel to front end position Setting the real time

2-7 2-7-1 2-7-2 2-7-3

Knife carriers Standard knife carrier Disposable blade carrier Disposable blade carrier EC 70

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

HM 525

2-8 2-8-1

Error code indication Definition of the error codes

2-9 2-9-1 2-9-2

Accessories Standard equipment Additional equipment

PART 3 THEORY OF OPERATION 3-1

Microtome cooling, defrost cycle

3-2

Cutting movement

3-3

Specimen coarse feed and trimming stages

PART 4 WORKING WITH THE CRYOSTAT 4-1

Preparations on the microtome and inside the cooling chamber

4-2

Freezing on the specimen

4-3

Orientation and trimming of specimens

4-4

Temperature list for cryo sectioning

4-5

Sectioning and taking off sections

4-6 4-6-1

How to avoid malfunctions Possible sources of errors – cause and removal

PART 5 MAINTENANCE AND CARE OF THE CRYOSTAT 5-1

Shutting-off for cleaning

5-2

Removing the microtome

5-3

Cleaning and care of the microtome

5-4

Cleaning the cooling lamella

part 6conditions for the transportation of the instrument 6-1

Taking back the instrument for repair or routine maintenance

6-2

Disposal of the instrument after final shutdown

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

HM 525

EC Certificate of Conformity Name and address of the manufacturer:

MICROM International GmbH Robert-Bosch-Straße 49 D-69190 Walldorf

Product designation: Type reference:

Microtome Cryostat HM 525

Notification to Competent Authorities: These medical device have been registered with the German authority as “Microtomes” under the EDMA-classification code: 23-06-02 The designated product complies with the laid down regulation: DIRECTIVE 98/79/EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 27 October 1998 on in vitro diagnostic medical devices The designated product complies with the EC regulations by strictly observing the following norms: DIN EN ISO 14971:2001-03

Medical devices - Application of risk management to medical devices (ISO 14971:2000).

DIN EN 61010-1:2002-08

Safety requirements for electrical equipment for measurement, control and laboratory use - Part 1: General requirements (IEC 61010-1:2001).

DIN EN 61010-2-101:2003-09

Safety requirements for electrical equipment for measurement, control and laboratory use - Part 2-101: Particular requirements for In-Vitro-Diagnostic-(IVD)-Medical instruments.

DIN EN 61326:2002-03

Electrical equipment for measurement, control and laboratory use - EMC requirements (IEC 613261:1997 + A1:1998 + A2:2000); German version EN 61326:1997 + A1:1998 + A2:2001

DIN EN ISO 9001:2000

Quality management systems - Requirements (ISO 9001:2000)

Hans Heid Managing Director MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

Walldorf, 15 June 2004

HM 525 SAFETY PRECAUTIONS

WARNING SIGNALS AND SYMBOLS The installation and routine use of the HM 525 is easy and safe if the instructions in this manual are being observed.

Note: Special instructions regarding operation of the instrument.

Warning: Special precautionary measures to prevent damage to equipment. For a long lifetime of the equipment, please observe these instructions carefully.

Caution – general danger spot: The instruction manual must strictly be observed whenever this symbol is visible on the instrument.

Biohazard: Warning of biological danger.

Radioactivity: Warning of radioactive danger.

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

HM 525 SAFETY PRECAUTIONS

CAUTION! The operator's safety is affected, when the instrument is not operated in accordance with this instruction manual. Please observe the following general precautions during operation of this instrument. Failure to comply with these precautions violates safety standards and the intended use of the instrument. MICROM International GmbH is not liable for misuse of the instruments and failure to comply with basic safety requirements.

INSTRUMENT GROUNDING To avoid injury from electrical current, the instrument must be connected with the protective earth. The instrument is equipped with a three wire ground plug. The power outlet must be connected to the protective earth and must meet the International Electrotechnical Commission (IEC) regulations.

CAUTION: MAINS VOLTAGE Never remove instrument covers during operation. Component replacements as well as adjustments must only be made by trained service personnel. Unplug the unit before removing or opening the covers.

DANGER IN EXPLOSIVE ENVIRONMENT The instrument must not be operated in the presence of flammable gases.

HAZARD OF FROSTBITE Avoid permanent touching of metal parts inside the cryostat microtome chamber as frostbite may occur at unprotected hands and arms.

HAZARD OF RADIOACTIVE RADIATION

When working with radioactive specimens observe all applicable radiation safety procedures. When working with radioactive contaminated material, appropriate safety and disinfection measures must be carried out. According to the rules and regulations concerning the handling of radioactive contaminated material of the respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must be worn. Radioactive contaminated waste must be disposed of according to the respective regulations.

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

HM 525 HAZARD OF INFECTION

Use the appropriate safety and disinfection measures when working with infectious specimens. According to the rules and regulations concerning the handling of infectious/radioactive contaminated material of the respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must be worn.

HAZARD OF BIOLOGICAL DANGER

Specimens used during the intended operation of the instrument might potentially be infectious. For this reason, it is recommended to observe the general laboratory regulations concerning protection against danger of infection. Information on decontamination media, their use, dilution and effective range of application can be read in the Laboratory Biosafety Manual : 1984 of the World Health Organization.

HAZARD OF MALFUNCTION To avoid the hazard of malfunction of an instrument, it must only be operated in a controlled electromagnetic environment. This means, that transmitters such as mobile phones must not be operated in their close vicinity. In case of malfunctions and/or service work, please turn off the instrument and contact your local dealer.

CARE IN USING MICROTOME KNIFE

To diminish the danger of being injured by the knife or blade, use the knife guard when adjusting specimen and knife. If possible, the specimen should be clamped in before the knife is inserted into the knife holder. Before changing the knife holder, always remove blade or knife! Unused knives should always be kept in a knife case. Never place the knife with the cutting edge upwards. Never try to catch a dropping knife!! Never check the sharpness of the cutting edge with your fingers. The cutting edge is extremely sharp!

WASTE DISPOSAL All debris, waste, defrosting liquid as well as infectious and radioactive contaminated material from operation must be disposed of in accordance with the respective regulations of the lab. Disinfection and cleaning liquids as well as section waste must be disposed of according to the respective regulations for special waste!

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

HM 525

1

INTRODUCTION

1-1 DESCRIPTION OF THE HM 525 Open top cryostat. Stainless steel cooling chamber. Chamber temperature regulation variable from +5°C down to –35°C. Graphic LED-display of set and actual temperatures, electronic control with user-oriented touchpad keyboard, battery-buffered memory and self-diagnostic system. Automatic defrosting and in addition a manual defrost cycle which can be activated when needed. Defrost interrupt on keystroke request. Integrated fast freezing device down to max. –60°C via Peltier element. Sliding window with heater. Integrated fluorescent lamp for cryo-chamber. Brush shelf and storage space inside the cryo chamber. Large, flat storage space on top of the housing with integrated storage space for marked slides. Rotary Microtome with backlash and maintenancefree cross roller bearings in stainless steel. Electromechanical feed system. Section thickness setting from 1 to 20 microns; up to 10 µm in 1 µm-increments, up to 20 µm in 2 µm-increments, Trimming thickness setting from 10 to 500 microns; up to 100 µm in 10 µm-increments, up to 300 µm in 50 µm increments and up to 500 µm in 100 µm-increments. Specimen retraction during the return stroke with optical indication. Horizontal feed range 28 mm. Vertical cutting stroke 60 mm. Max. specimen size 70 x 55 mm. Motorized coarse feed in two directions. Limit indication and automated switch off at front and rear limits of horizontal travel. Section counter and indication of sum of section thicknesses with reset-button. Indication of remaining travel. Mechanical handwheel brake in the lower reversal point of the specimen orientation.

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

Fine orientation of specimen 8° universally in x/yaxis with zero device. Rotatable on Z-axis, 360°. Basic outfit with 3 x 30 mm chucks, 1 x 40 mm chuck, 118 ml MICROM freezing medium, 100 ml cryostat oil, brush shelf and section waste tray, consisting of two parts.

HM 525

1-2

TECHNICAL DATA HM 525

Chamber temperature control:... +5°C to -35°C Fast freezing station via Peltier element depending on the chamber temperature: ...down to max. -60°C Defrosting:

automatic defrosting, adjustable ... with temperature control manual immediate defrosting

Microtome:

Section thickness range: ... 1 - 500 µm Fine section thickness range: ... 1 - 20 µm Resolution: ... 1 µm for 1 - 10 µm ... 2 µm for 10 - 20 µm Trimming thickness range: ... 10 - 500 µm Resolution: ... 10 µm for 10 - 100 µm ...50 µm for 100 - 300 µm ... 100 µm for 300 - 500 µm Specimen retraction during return travel... 40 µm Vertical specimen stroke ... 60 mm Horizontal specimen movement ... 28 mm

Read-outs:

Five-digit LED-display: section thicknesses, section counter, sum of section thicknesses, remaining travel to front end position and chamber temperature

Coarse feed:

... motorized, graduated, single and continuous

Size of chucks:

...30, 40 mm ... special sizes upon request x - and y - axes: ... universal 8° with zero positioning z - axis: ... up to 360°

Specimen orientation:

Cooling chamber illumination Sliding window Cutting drive: Cutting area:

... manually via handwheel ... horizontal specimen movement 28 mm ... vertical specimen stroke 60 mm

All temperatures refer to an ambient temperature of +20°C! Cont'd on page -2-

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

2

HM 525

page -21-2

TECHNICAL DATA HM 525

Handwheel brake:

...mechanical

Transportation and storage conditions Storage temperature range: -20°C up to +50°C Operating conditions:

... +5°C up to +35°C (at a max. rel. humidity of 60%) ... altitude up to 2000 m M.S.L. ...for indoor use only ... Floor loading requirements: 230 kg/m2

Power requirements:

... 100 V/50 Hz, 12 A, +/-10% ... 100 V/60 Hz, 12 A, +/-10% ... 115 V/60 Hz, 12 A, +/-10% ... 220...230 V/50 Hz, 6 A, +/-10% ...220 V/60 Hz, 6 A, +/-10% ...240 V/50 Hz, 6 A, +/-10%

Internal protection Primary circuits: Transformer: Illumination:

Secondary circuits: Window heating: CPU: Panel: Stepping motor/valve: Fast freezing station:

...T5.0AH, 100...115 V ...T2.5AH, 220...240 V ...T0,8AH, 100...115 V ...T0,4AH, 220...240 V

... T2,5AH ... 11 VAC/T0.5AH ... 11 VAC/T0.5AH ... 24 VAC/T2.5AH ... 2.3/8 VAC/T8.0AH

Pollution degree: Overvoltage category: Sound pressure:

... 2 ...II ... 45 dB(A) ... measured with 1 m distance to the instrument

Fluids and gases:

...cooling agent: R404a, ... filling amount: 100...120 V: 250 g ... 220...240 V: 230 g

Dimensions:

... Wide (w/o handwheel): 640 mm, deep: 760 mm, high: 1200 mm

Weight:

... 143 kg (depending on the model)

All temperatures refer to an ambient temperature of +20°C!

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

3

HM 525

1 11

2

10

17

4

18

6

7

8

9

10 12

9

16

14

5

18

3

20

2

22

COUNT

SECTION THICKNESS µm

SUM

RETRACT

P

15

DOWN

UP

14

500

10 20

400

30

300

40

MEMO

DEFR TIME

INTER RUPT

250 50

7

HM 520

CHAMBER TEMPERATURE ° C

TRIM THICKNESS µm

TRAVEL

8

MICROM

16

4

60

70 80 100 150 200

6

5 3

13 12 Fig. 2

1 = LED retraction 2 = Section thickness setting 3 = Trimming thickness setting 4 = LED fine feed 5 = LED trim feed 6 = Coarse feed, backwards 7 = Coarse feed, forwards 8 = Trim button 9 = Scroll button

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

10 = Display, left 11 = Reset button 12 = Memory button 13 = Setting defrost time 14 = Interruption defrosting cycle 15 = Down button and activation of Peltier element 16 = Up button 17 = Chamber illumination 18 = Display, right

4

HM 525

2

OPERATING INSTRUCTIONS

2-1

SETTING UP THE CRYOSTAT 1

Unpacking the instrument: ??Cut through the three packing straps and remove the packing. ??Remove the upper wooden cover (fig. 3.1). ??The hinges of the upper wooden cover are secured with tape. ??Remove this tape. ??Lift the packing (fig. 3.2) over the instrument. ??Remove the upper and lower supporting foams (fig. 3.3, 3.4, 3.5, 3.6) from the cryostat.

2

3

Note: The upper wooden cover now serves as an incline (fig. 3a.1) on which the cryostat can be moved from the palett to the floor by using the handle (fig. 3a.2) ??The hinges of the upper wooden cover (fig. 3.1) are inserted into the screws of the bottom plate (fig. 3a.3). The correct side for this is marked with arrows. ??The tool (fig. 3b.2, size 30) for the height adjustment of the setting feet of the cryostat is packed together with the accessories of the cryostat (separate carton on the foot rest of the cryostat). ??Turn the setting feet completely upwards via this tool so that the cryostat stands with its rollers on the bottom plate (fig. 3a.3). ??Via the handle (fig. 3a.2) roll the cryostat from the bottom plate (fig. 3a.3) over the wooden cover (fig. 3a.1). In doing so, the rear side of the instrument must show to the incline (fig. 3a.1). Caution: The instrument is very heavy. While the instrument rolls down on the incline, it must be guaranteed that the instrument does not move in an uncontrolled way.

HM

55

0M

VD

5 0

3

2

1

4

6 7

Fig. 3

HM550M VD

2 3

10

2

1

3

Fig. 3a

??Now the cryostat can be rolled to its site of installation. ??For possible return shipments, please keep the packing.

1

2 Fig. 3b MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

5

HM 525

Warning: The instrument must only be moved in standing or slightly tilted (approx. 30°) position. Choose installation site that ??enough ventilation for the cooling system is guaranteed. ??the distance between wall and rear panel is approx. 15 cm. For this, install the distance bolts (fig. 4a.1). ??the suction areas (fig. 4a.2) on either side are kept free. ??the mains switch for separating the instrument from the power supply is accessible any time.

1

SW13

Moreover, the installation site must be free from: ??draught by open doors or by air conditioning systems. ??direct exposure to sunlight into the cooling chamber.

Fig. 4

Note: Both measures reduce the formation of frost and therefore result in more favourable work conditions. A high air moisture as well as high ambient temperatures reduce the maximum performance of the instrument. ?? To fix the complete unit, tighten the screws (fig. 4.1) by using the attached tool. ?? Install the separate packed handwheel handle.

1 2

Fig. 4a

Warning: Instead of the handwheel handle, a transportation screw (fig. 4b.1) is inserted into the handwheel. This way, the handwheel is tightly and securely connected with the housing of the cryostat. It is absolutely necessary to remove this transportation screw before the initial turnon! ?? Loosen the transportation screw (fig. 4b.1) via the Allan key, size 6. ?? Now fix the separately packed handwheel handle on the handwheel with the attached screw by means of the Allan key.

Fig. 4b 1

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

HM 525 2-2

INITIAL TURN-ON

Note: The kind of the used examination materials and all special conditions for their processing, pre-treatment and, if necessary, storage as well as instrument controls for correct and safe operation is in the responsibility of the operator. The operator is also responsible for special equipment and materials and/or reagents for the operation of the instrument. Warning: Before turning on the instrument for the first time, please check if the power requirements indicated on the type plate (fig. 6) correspond to the power supply voltage being used. ??No other instruments should be connected to the circuit used for the cryostat, as the compressor needs high surge currents when started. ??Do not use multi-socket power outlets with small wire sizes for the supply of the instrument. ??We recommend to immediately install all accessories belonging into the cryo chamber, e.g. blade carrier. ??The instruction manual of the instrument can be found in the separate carton which is placed on the foot part of the cryostat. ??Connect the power line of the instrument to the power outlet. ??Turn on the power switch (fig. 5.1) on the rear side of the instrument. ??Close the heated sliding window and cool down the instrument. The cooling phase will take several hours depending on the set chamber temperature.

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

I 0

2

1 Fig. 5

1 = Power switch 2 = Mains plug

MICROM GmbH 69190 Walldorf, Germany

Type HMHM 520_ Type 525 Ser. No. _ _ _ _ Cat. No. _ _ _ _ _ _ _ _A /_ _ _V _ _ Hz

Chamber Refr.: R 404a Amount: _ _ _ _ _kg Max. Press.: 1900kPa Made in Germany

IVD

Fig. 6

HM 525 2-3

BASIC OPERATIONAL RULES

After having turned on the instrument, the two displays show the set chamber temperature as well as the number of sections, the section thickness sum as well as the remaining travel to the front end position. Section thicknesses are indicated via LEDs.

2-3-1

1

ACTUAL AND SET VALUE OF THE TEMPERATURE

CHAMBER TEMPERATURE ° C P

DOWN

UP

The cooling of the microtome chamber can be controlled by the corresponding part of the operating controls. The actual temperature of the microtome chamber is shown in °C on the digital display (fig. 7.1). Briefly press the UP- or DOWN-button (fig. 7.2 or 7.3), the set value of the temperature in the microtome chamber is shown for approx. 2 sec. Afterwards, the display shows again the actual value of the temperature of the microtome chamber. To change the set value, press permanently the UP-button (fig. 7.2) or DOWN-button (fig. 7.3). The UP-button leads to higher, the DOWN-button to lower temperatures. The valid range of the set value goes from +5°C down to -35°C. If the UP-button is used beyond the limit of +5°C, the display shows " - - -" and the temperature control is turned off. Using the DOWN-button the cooling system is turned on again.

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

2

MEMO

DEFR TIME

INTER RUPT

3

Fig. 7

HM 525 2-3-2

DEFROSTING THE EVAPORATOR

Every 24 hours the evaporator in the rear part of the microtome chamber is defrosted automatically. During a defrosting process, the display shows a temperature with the letter H. This temperature refers to the heating of the evaporator and not to the temperature of the cooling chamber, which is normally shown. This value, however, is of no importance for the operation of the instrument. Press the button DEFR TIME (fig. 8.4) and the set defrosting time is shown for approx. 2 sec. on the display (fig. 8.1). It is advisable to set the time of the defrosting process not during routine working time. To change the starting time of defrosting at intervals of 15 minutes, simultaneously press the button DEFR TIME (fig. 8.4) and the button UP (fig. 8.2) or DOWN (fig. 8.3). Defrosting can only be carried out at the set defrosting time, if the real time is set correctly. After finishing the adjusting process, the display shows the set temperature of the microtome chamber after two more seconds. Note: An immediate defrosting cycle can be released at any time. For this, press the button DEFR TIME (fig. 8.4) and additionally the reset button (fig. 2.11).

2-3-2

INTERRUPTING A DEFROSTING CYCLE

If needed, the daily defrosting can be interrupted or cancelled. Press the button INTERRUPT (fig. 8.5), the LED in this button lights up. If the function INTERRUPT is chosen during the defrosting period, the LED blinks for the rest of the suppressed defrosting process. A cancelled or interrupted defrosting process must be repeated later on, as otherwise the evaporator will cover completely with frost and cannot cool any more. For this, press the button (fig. 8.5) again to turn off the function INTERRUPT (LED is off). The next defrosting process will then be started when the next pre-selected defrosting time is reached.

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

HM 525 2-3-3

EMPTYING THE DEFROSTING LIQUID

Note: The liquid from the daily defrosting process is collected in the container (fig. 9.1). Please note that this container is emptied in due time according to the safety precautions in this instruction manual. We highly recommend to disinfect the container with a surface disinfection solution after having emptied it.

1

Fig. 9

2-3-5

ILLUMINATION OF THE COOLING CHAMBER

To illuminate the microtome chamber, a fluorescent lamp is above the chamber, which can be turned on and off by means of the button (fig. 10.1). At low temperatures and after long periods of being turned off, the lamp might flare when turning it on.

1

CHAMBER TEMPERATURE ° C

2

DOWN UP

MEMO

DEFR TIME

INTER RUPT

Fig. 10

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

HM 525 2-3-6

FREEZING-ON OF SPECIMENS

There are several possibilities to freeze-on specimens. Various specimen chucks are available. Round specimen chucks can be supplied with a diameter of 30 mm and 40 mm and rectangular specimen chucks with a size of 50, 55, 60 and 70 mm. Special sizes on request.

18

27

17

26

9 8

16

25

15

24

7 6

14

23

13

22

5

4

12

21

11

20

3

2

1

10

19

For the especially fast and almost artefact-free freezing-on, the HM 525 is equipped with a Peltier element (fig. 12.1) (down to max. –60°C surface temperature). 1

Fig. 11

?? Briefly press the down button (fig. 10.2) twice to activate the Peltier element. ?? Put the warm chuck into the hole (fig. 11.1) of the fast freezing rail. ?? Put freezing medium onto the chuck. ?? Place fresh tissue onto the still liquid freezing medium. ?? When the medium and tissue are frozen, the chuck can be inserted into the specimen clamping and can be fixed. ?? The specimen can now be sectioned. ?? The remaining 23 stations of the fast freezing station can also be used for freezing-on or storing specimens. 1 Note: The upper side of the freezing station must not be covered by frost or ice. To avoid this, use ethanol or the like.

Fig. 12

Note: The Peltier element automatically turns off after 8 min.

Caution: With regard to hazard of frostbite, it is absolutely necessary to observe the safety precautions listed at the beginning of this manual.

Fig. 13

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

HM 525 2-3-8

SPECIMEN ORIENTATION 2

In many cases, the orientation of the specimen in relation to the cutting edge would be advantageous. This can easily be done by means of the orienting specimen holder on the microtome.

1

?? Loosen the clamping lever (fig. 15.1). ?? Via the orienting lever (fig. 15.2) the specimen clamping can be moved in all directions by approx. 8°. ?? After having achieved the desired alignment, fix the position via the clamping lever. ?? The orienting lever is also used for clamping the chuck into the orienting specimen holder. Note: The specimen orientation includes a zero device for the specimen (chuck is always parallel in relation to the knife). Zero position can be felt noticeably.

MICROM International GmbH Robert-Bosch-Str. 49 D- 69190 Walldorf

Fig. 15