BX46 CLINICALMICROSCOPE Instructions Sept 2013

In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment, this symbol indicates that the product must not be disposed of as unsorted municipal waste, but should be collected separately. Refer to your local Olympus distributor in EU for return and/or collection systems available in your country.

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense. FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance could void the user's authority to operate the equipment.

For Korea only B급 기기

(가정용 방송통신기자재)

이 기기는 가정용(B급) 전자파적합기기로서 주로 가정에서 사용하는 것을 목적으로 하며, 모든 지역에서 사용할 수 있습니다.

BX46

CONTENTS

Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section 10, “ASSEMBLY" (pages 31 to 34) carefully.

IMPORTANT -- Be sure to read this section for safe use of the equipment. --

1-3

1

MODULE NOMENCLATURE

2

CONTROLS

5-7

3

FLOW OF OBSERVATION

8,9

4

SIMPLIFIED OBSERVATION PROCEDURE

4

10-12

4-1 Basic Operation (Until Observation of Specimen)...10,11 4-2 Microscope Adjustments (How to Improve the Observed Image)...11,12

5

1 Adjusting the Interpupillary Distance... 11

2 Adjusting the Diopter... 11

3 Adjusting the Centering...12

4 Adjusting the Contrast...12

USING THE CONTROLS

13-23

5-1 Base...13,14 1 Adjusting the Brightness...13

2 Setting the LIM Brightness...13

3 Using the Filters...14

5-2 Focusing Block...15,16 1 Focusing Controls... 15

2 Adjusting the Focus... 15

3 Replacing the Fine Adjustment Knob...16 5 Pre-focusing Lever...16

4 Adjusting the Coarse Adjustment Knob Tension...16

5-3 Stage... 17,18 1 Placing the Specimen... 17

2 Adjusting the X- and Y-Axis Knob Tension... 18

3 Rotating the Stage... 18

5-4 Observation Tube... 19-22 1 Adjusting the Diopter...19

2 Using the Eye Shades...19

3 Mounting the Eyepiece Micrometer Disk... 20

4 Selecting the Light Path of the Trinocular Tube... 20 6 Using Eyepieces Incorporating a Micrometer... 22

5 Adjusting the Tilt... 21

5-5 Condenser... 22 5-6 Immersion Objectives... 23

6

CAMERA RECORDING

7

TROUBLESHOOTING GUIDE

25-27

8

SPECIFICATIONS

28,29

9

OPTICAL CHARACTERISTICS (UIS2 Series)

10

ASSEMBLY -- See this section for the replacement of the light bulb. --

11

HALOGEN LAMP SOCKET INSPECTION SHEET

24

30 31-34 35

n PROPER SELECTION OF THE POWER SUPPLY CORD... 36,37

BX46

IMPORTANT SAFETY PRECAUTIONS

(Fig. 1) 1. If potentially infectious samples may be observed, use protective gloves or other protective means to prevent the skin from contacting with samples directly. After observation, be sure to clean the portion contacted with samples. · Moving this product is accompanied with the risk of dropping the samples. Be sure to remove the samples before moving this product. · In case the samples is damaged by erroneous operation, promptly take the infection prevention measures. · Follow the procedures described in Chapter “Getting Ready” (see on page 2) prior to using the accessories of this product. Otherwise, the stability of the device will be lost and the dropped samples will cause the possibility of infection. · When you maintain the device which may have contacted with potentially infectious reagents, be sure to wear the protectors such as gloves, or clean the device prior to operation. · Before disposing of device contacted with potentially infectious samples, be sure to follow the regulations and rules of your local government. 2. Install the microscope on a sturdy, level table or bench. 3. Be careful not to tilt the microscope too much. Otherwise, the mobile parts such as the light path selector knob may move in unintended directions. 4. Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the section “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual. If the proper power cord is not used, product safety performance cannot be warranted. 5. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected. If the equipment is not grounded, Olympus can no longer warrant the electrical safety performance of the equipment.

Operation Using the LED Lamp Safety Note on LED (Light Emitting Diode) The LED incorporated in the LED lamp housing is a class 1 LED product. The light emitted by LED is basically safe, but do not look at the illumination light directly for an extended period to prevent your eye from being injured CLASS 1 LED PRODUCT

Operation Using the Halogen Bulb 1. To avoid potential shock hazards and burns when replacing the halogen bulb, set the main switch of the TL4 power supply unit to “ ” (OFF) and allow the lamp socket @ and bulb to cool before touching them. Designated bulb CAUTION

1 Fig. 1

6V30WHAL (PHILIPS 5761)

Always use the designated lamp bulb. Using other bulb may cause malfunction.

2. If the lamp cable or power cord comes in contact with the lamp socket or its surroundings, the cable or cord may be melted by the heat, causing an electric shock or fire hazard. To prevent this, distribute the lamp cable and power cord at enough distances from the lamp socket. 3. The standard service life of the lamp socket is eight (8) years of use or 20,000 hours of total power ON period, whichever is the shorter period. For details, see Inspection Sheet on page 35.

1

Restrictions in Use When all combinations (@ to ³) below are satisfied at the same time, the microscope may fall if tilted larger than a certain degree, comparing to other combinations. Please mount the camera port of the intermediate observation tube toward diagonally backward 45° to prevent from falling. @ The observation tube is either the trinocular tube, U-TTBI, U-ETBI or U-TTLBI. ² The intermediate observation tube is either U-TRUS or U-DP. ³ The camera heavier than 0.4kg is mounted on the intermediate observation tube described in ².

Safety Symbols The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in the safest possible manner. Symbol

Explanation Indicates that the surface becomes hot, and should not be touched with bare hands. Indicates a non-specific general hazard. Follow the description given after this symbol or in instruction manual. Indicates that the main switch is ON. Indicates that the main switch is OFF.

Caution engraving/label Caution engraving and label are placed at parts where special precaution is required when handling and using the microscope. Always heed the cautions. Caution engraving position Caution label position

Halogen lamp socket [Caution against high temperature] Rear panel [Caution against high temperature]

If a caution engraving or label is dirty or peeled off, contact Olympus for the replacement or other inquiry.

1

Getting Ready

(Fig. 2) 1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact. Also note that the microscope does not have a waterproof construction. 2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For the operating conditions, refer to Chapter 8, “SPECIFICATIONS”.) 3. When moving the microscope, remove the observation and specimen and carefully carry the microscope by holding the grasping part on the rear of the arm and the front part of the base as shown in Fig. 2. CAUTION

Fig. 2

2

To prevent damage to the microscope, never hold it by the stage or coarse/fine adjustment knob.

· Even when moving the microscope along the desktop surface, be sure to remove the specimen in advance. · When the microscope needs to be packaged for forwarding to a remote location, contact Olympus. 4. When the accessories of this product are used, make sure that the system height does not exceed 1 meter. Otherwise, the stability of the device will be lost. 5. When an arm, illuminator, intermediate attachment, observation tube and camera are to be attached to the upper part of the microscope frame, make sure that their total weight does not exceed 7 kg.

BX46

2

Maintenance and Storage 1. To clean the lenses and other glass components, simply blow dirt away using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute alcohol.

CAUTION

· Since the absolute alcohol is highly flammable, it must be handled carefully. Be sure to keep it away from open flames or potential sources of electrical sparks --- for example, electrical equipment that is being switched on or off, which could cause ignition of a fire. Also remember to always use it only in a well-ventilated room. · Set the main switch to “ ” (OFF) and wait for the lamp housing to cool down sufficiently before proceeding to maintenance.

2. Do not use organic solvents to clean the microscope components other than the glass components. To clean them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent. 3. Do not disassemble any part of the microscope except for the parts that are specified to be disassembled in this manual, as this could result in malfunction or reduced performance. 4. After using the microscope, set the main switch to “ ” (OFF), (wait for the lamp socket to cool down sufficiently if the halogen bulb has been used,) and keep it covered with a dust cover during storage. 5. Before disposing of this product, be sure to follow the regulations and rules of your local government.

3

Caution If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual. The following symbols are used to set off text in this instruction manual. CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in minor

}

4

or moderate injury or damage to the equipment or other property. It may also be used to alert against unsafe practices. : Indicates commentary (for ease of operation and maintenance).

Intended use This product has been designed to be used to observe magnified images of specimens in various routine work and research applications. This includes the observation of living cells or of specimen taken from tissues to gain physiological or morphological information at hospitals or laboratories. Typical field of applications are genetics, human blood and tissue examination, neurology, pharmacology and cellular biology. Do not use this instrument for any purpose other than its intended use. This product complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical devices. CE marking means the conformity to the directive.

Safety and EMC Precautions · This product complies with the emission and immunity requirements described in IEC61326 series. · The electromagnetic environment should be evaluated prior to operation of this product. Do not use this product in close proximity to the sources of strong electromagnetic radiation to prevent interference with the proper operation. · Use only power cord which OLYMPUS specifies. Otherwise the safety and EMC performance of the product can not be assured. · Be sure to ground the product. Otherwise our intended electric safety and EMC performance of the product can not be assured.

3

1 MODULE NOMENCLATURE } The modules mentioned below show only the typical product names. As there are some products that are not mentioned but also applicable to this microscope, check the latest catalogues or consult Olympus. For the products marked “ * ”, also read their instruction manuals.

Eyepieces

Observation tube

· WHN10X · WHN10X-H

· U-TTLBI · U-TBI-3 · U-BI30-2

Intermediate attachment · U-TRU · U-CA · U-DO3

Lamp housing · U-LH100-3 · U-LS30ADP + U-LS30-5 + TL4*

Slide holder · U-HLST-4 · U-HLS-4

Stage · U-SVRC · U-SVRC-CY

4

2 CONTROLS

BX46

· If you have not yet assembled the microscope, read section 10, “ASSEMBLY” (pages 31 to 34).

Interpupillary distance adjustment scale (Page 11)

Diopter adjustment ring (Page 19)

Allen screwdriver holder

Slide holder (Page 17)

Coarse adjustment knob (Page 15)

Aperture iris diaphragm ring (Page 12)

Y-axis knob (Page 18) X-axis knob (Page 18) Filter slider insertion slot

Coarse adjustment tension adjustment ring (Page 16)

Field iris diaphragm ring (Page 12)

5

Main switch (Page 10) I : ON : OFF Fine adjustment knob (Page 15) Detachable. LIM setting switch (Page 13)

Pre-focusing lever (Page 16)

LED brightness adjustment knob (Page 13)

Top lens swing-out lever (Page 12) Condenser height adjustment knob (Page 12)

6

LIM ON-OFF switch (Page 13) LIM indicator ON : Lit (Green) OFF : Extinguished

BX46

<< Modules for halogen lamp operation >> LS30 Adapter U-LS30ADP

Halogen Lamp Socket U-LS30-5

Guide pins

Guide pin holes

Lamp cable

Power Supply Unit TL4 Lamp socket connector

Brightness control knob

Main switch I : ON.

: OFF.

7

3 FLOW OF OBSERVATION } When the LED lamp is used and the LIM is set, the LED brightness adjustment knob is defeated. } When the halogen bulb is used, set the 32LBD filter in the filter slider and insert it into the insertion slot @.

(Controls Used)

Set the main switch to “ I ” (ON) and adjust the brightness.

Select the light path (trinocular tube).

LED lamp

Halogen bulb

@ Main switch 2 LED brightness adjustment knob*

3 Main switch 4 Brightness adjustment knob

(P. 13)

5 Light path selector knob

(P. 20)

6 Slide holder 7 X-/Y-axis knobs

(P. 17) (P. 18)

8 Revolving nosepiece 9 Top lens swing-out lever

(P. 12)

a Coarse/fine adjustment knobs

(P. 15)

b Binocular tube c Diopter adjustment ring d Condenser height adjustment knob e Condenser centering screws

(P. 11) (P. 19) (P. 12) (P. 12)

Adjust the aperture iris and field iris diaphragms.

f Aperture iris diaphragm ring g Field iris diaphragm ring

(P. 12) (P. 12)

Engage the objective to be used in the light path and bring the specimen in focus.

8 Revolving nosepiece 9 Top lens swing-out lever

(P. 12)

h Filter mount

(P. 14)

Place the specimen on the stage.

Engage the 10X objective in the light path.

Bring the specimen in focus.

Adjust the interpupillary distance. Adjust the diopter. Adjust the light axis.

Engage the required filters.

Adjust the brightness.

2 LED brightness adjustment knob*

Start observation. * The LED brightness adjustment knob is defeated when the LIM is set (see page 13).

8

(Page)

4 Brightness adjustment knob

(P. 13) (P. 13)

BX46

5 (trinocular tube only)

c

1

b

8 6

f e

7 2

h

g

4

3 9 a d

} Copy the observation procedure pages on separate sheets and post it near your microscope.

9

4 SIMPLIFIED OBSERVATION PROCEDURE 4-1 Basic Operation (Until Observation of Specimen) This section describes the basic operation of the microscope until the start of observation of a specimen. For the detailed operating procedure of each control, please read the description page specified below.

1

Press the main switch of the microscope frame to “ I ” (ON).

2

Rotate the knob to adjust the lamp brightness. (Details: Page 13)

3

Rotate the knob to lower the stage. (Details: Page 16)

4

Open the clamping lever of the slide holder and place the specimen slide. (Details: Page 17)

1

Fig. 3

2 Fig. 4

4

3 Fig. 5

10

BX46

5

5

Rotate the revolving nosepiece to engage the 10X objective in the light path.

6

Rotate the coarse and fine adjustment knobs to bring the specimen in focus. (Details: Page 19)

7

Rotate the stage knob to adjust the observation position.

7 6

Now you can observe the magnified image of the specimen. To improve the observation further, read section 4-2, “Microscope Adjustments” below.

Fig. 6

4-2 Microscope Adjustments (How to Improve the Observed Image) Adjust the microscope as described below to improve the observed image.

1

Adjusting the Interpupillary Distance 1

1

While looking through the eyepieces, adjust for binocular vision until the left and right fields of view coincide completely. } Note your interpupillary distance so that it can be quickly duplicated.

Fig. 7

2

Adjusting the Diopter The diopter adjustment ensures parfocality from low to high magnifications and makes it possible to avoid defocusing even when the objective is switched. For details, see page 19.

Fig. 8

11

3

Adjusting the Centering 8 2 1

4

3

5

7

10 11

6

1

Place the specimen.

2

Rotate the revolving nosepiece to select the 10X objective.

3

Raise the lever to engage the top lens in the light path.

4

Rotate the knobs to bring the specimen in focus.

5

Rotate the knob to raise the condenser to its upper limit.

6

Rotate the field iris diaphragm ring in the direction of the arrow so that the diaphragm image comes inside the field of view.

7

Rotate the knobs to focus on the field iris diaphragm image (A in Fig. 9).

8

Pull out the two condenser centering screws.

9

Insert the two condenser centering screws into the condenser centering thread holes and turn the two condenser centering screws to move the iris diaphragm image to the center of the field of view (B in Fig. 9).

9

10 Gradually open the field iris diaphragm until its image is inscribed in the field of view (C in Fig. 9). 11 Open the field diaphragm slightly until its image circumscribes the field of view slightly (D in Fig. 9). } With the 100X objective, the field iris diaphragm image cannot be observed unless the iris diaphragm is minimized. With the 4X objective, maximize the iris diaphragm to observe it.

A

B Fig. 9

4

C

D

Movement of Field Iris Diaphragm Image

Adjusting the Contrast Aperture iris diaphragm image

70-80% 30-20%

Objective pupil

1

12

NA scale

2

1

Set the condenser aperture iris diaphragm ring so that the condenser NA scale position indicates 70% of the NA of the objective in use. After this, observe the image and fine adjust the aperture iris diaphragm to the desired condition.

2

The image of the aperture iris diaphragm can be viewed by removing the eyepieces and looking into the observation tube. Adjust so that the image looks approximately like that shown in the figure above.

5 USING THE CONTROLS

BX46

5-1 Base 1

Adjusting the Brightness

(Figs. 10 & 11)

1. When the LED lamp is used for observation, turn the LED brightness adjustment knob @ clockwise to make illumination brighter. 2. When the halogen bulb is used for observation, adjust the brightness adjustment knob 2 of the TL4 power supply unit.

1 Fig. 10

2

Fig. 11

2 2 1 3

4 Fig. 12

Setting the LIM Brightness

(Fig. 12)

} LIM stands for Light Intensity Manager. It is effective when the U-LHLEDC LED lamp housing is used. } The desired brightness can be set per objective. Once the brightness for the objectives is set, selecting an objective adjusts the LED brightness automatically provided that the LIM ON-OFF switch @ is set to “ON” (which is indicated by the lighting of the LIM indicator 2). 1. Press the LIM ON-OFF switch @ to “OFF ” (so that the LIM indicator 2 is extinguished). 2. Engage a low-power objective in the light path and bring the specimen in focus. 3. Rotate the brightness adjustment knob 3 for optimum brightness for observation. 4. Press the LIM setting switch 4 to enter the current brightness. 5. Set the optimum brightness for the mounted objectives by repeating the above for each of them. To modify the LIM setting: Engage the objective you want to set the new LIM setting in the light path, set the LIM ON-OFF switch to “OFF”, and perform steps 3 and 4 above.

13

1

2

Fig. 13

3

Using the Filters

(Fig. 13)

} One of the filters listed below can be engaged in the light path by inserting the filter @ in the filter slider 2 and engaging the filter slider in the light path. Usable Filters

Applications

32LND1.5/3/6/12/25/50

For light brightness control, transmittance 1.5/3/6/12/25/50%

32LBD

For color temperature conversion

32IF550

For B&W contrast (Green)

} The LBD filter is not required when the LED lamp is used.

What is LBD filter? The color of the sun at the noon of a clear day is called the daylight color, which looks white to human eyes. On the other hand, the halogen bulb used as the light source of this microscope does not emit the daylight-color light. so the LBD filter is engaged in the light path to convert the color of halogen bulb light into daylight. In general, engaging the LBD filter makes it possible to observe specimens in natural color tones.

14

BX46

5-2 Focusing Block } The stage of this microscope is fixed at a low height to facilitate replacement of specimens. Take care not to let your hand and fingers interfere with the stage when operating the coarse adjustment knob.

1

Focusing Controls

(Figs. 14 & 15)

1

The focusing block consists of the controls listed in the following table, which also describes the functions of the controls.

2

No.

Name

1

Coarse adjustment knob

2

Fine adjustment dial Fine adjusts the focus position.

3

Fine adjustment knob

Fine adjusts the focus position. This knob can be attached to the fine adjustment dial on either the left or right side. (When the microscope is shipped, it is attached to the right-side dial.)

4

Pre-focusing lever

Sets the lower limit of the objective position (to prevent contact between the specimen and objective).

5

Tension adjustment ring

Adjusts the force required to rotate the coarse adjustment knob.

Adjusting the Focus

(Fig. 16)

5 Fig. 14

1

3

Function Moves the focus position widely.

4 Fig. 15

2

1

With both the coarse adjustment knob @ and fine adjustment knob 2, rotating the knob in the direction of the arrow raises the objective (lets the specimen approach the objective).

2

Fig. 16

15

3

Replacing the Fine Adjustment Knob

CAUTION

4

3 5 Fig. 17

4

1

Adjusting the Coarse Adjustment Knob Tension

CAUTION

5

Fig. 19

16

(Fig. 18)

Adjust the coarse adjustment knob tension using the tension adjustment ring @.

The coarse adjustment knob tension is preadjusted for easy use. However, if desired, you can change the tension using the tension adjustment ring @. Turning the ring in the direction of the arrow increases tension, and vice versa. The tension is too low if the stage drops by itself of focus is quickly lost after adjustment with the fine adjustment knob. In this case, turn the ring in the direction of the arrow to increase tension.

Fig. 18

1

The fine adjustment knob has been attached on the left side at the factory.

} The fine adjustment knob is designed detachable to prevent interference with hand during manipulation of the X-and Y-axis knobs. Usually attach the fine adjustment knob on the opposite side to the X- and Y-axis knobs. 1. Pull and remove the rubber cap @ from the fine adjustment knob. 2. Using the Allen screwdriver, loosen the clamping screw 2 and remove the fine adjustment knob 3. 3. Remove the seal from the fine adjustment knob screw hole on the other side and attach the knob by reversing the removal procedure. 4. Attach a provided seal on the screw hole 5 of the fine adjustment dial |, from which the fine adjustment knob has been removed. } The fine adjustment dial | can be operated with your fingertip or finger surface at the same time as manipulating the X- and Y-axis knobs.

1

2

(Fig. 17)

Pre-focusing Lever

(Fig. 19)

} The pre-focusing lever ensures that the objective does not come in contact with the specimen and simplifies focusing. After focusing on the specimen with the coarse adjustment knob, turn this lever @ in the direction of the arrow and lock; the lower limit on coarse adjustment movement is set at the locked position. After changing a specimen, approximate refocusing is easily accomplished by rotating the coarse adjustment knob to reach the pre-focused position. After this, all you have to do is make fine adjustments with the fine adjustment knob. } The objective’s vertical movement activated by the fine adjustment knob is not locked. } When the pre-focusing lever is locked, a coarse movement stroke is reduced due to the microscope’s mechanism, causing the revolving nosepiece not to be able to be lowered to the lower limit. To lower the revolving nosepiece to the lower limit, release the pre-focusing lever.