BioRobot EZ1 DSP Workstation User Manual Ver 1 Feb 2007

Trademarks: QIAGEN®, BioRobot® (QIAGEN Group); Gigasept®, Lysetol®, Mikrozid® (Schülke & Mayr GmbH); DECON-QUAT® (Veltek Associates, Inc.). © 2007 QIAGEN, all rights reserved.

Contents

Contents 1

Safety Information

1-1

1.1

Proper use

1-2

1.2

Electrical safety

1-2

1.3

Environment

1-4

1.4

Biological safety

1-5

1.5

Chemicals

1-6

1.6

Waste disposal

1-6

1.7

Mechanical hazards

1-7

1.8

Heat hazard

1-7

1.9

Symbols on the BioRobot EZ1 DSP

1-8

1.10

Additional symbols in this user manual

1-9

2

Introduction

2-1

2.1

About this user manual

2-1

2.2 2.2.1 2.2.2 2.2.3

General information Technical assistance Policy statement Version management

2-1 2-1 2-2 2-2

2.3 2.3.1

Intended use of the BioRobot EZ1 DSP Requirements for BioRobot EZ1 DSP users

2-2 2-3

3 3.1 3.1.1 3.1.2 3.1.3 3.1.4 3.1.5

General Description External features of the BioRobot EZ1 DSP Workstation door Control panel EZ1 Card slot Status LEDs Connector panel

BioRobot EZ1 DSP Workstation User Manual 02/2007

3-1 3-2 3-2 3-2 3-3 3-4 3-5

Contents-1

Contents 3.2 3.2.1 3.2.2 4

Internal features of the BioRobot EZ1 DSP Worktable Pipettor head Installation Procedures

3-5 3-6 3-9 4-1

4.1

Requirements

4-1

4.2

AC power connection

4-1

4.3

Hardware installation

4-3

5

General Operation

5-1

5.1

Overview

5-1

5.2 5.2.1 5.2.2

Inserting and removing the EZ1 Card Inserting the EZ1 Card Removing the EZ1 Card

5-2 5-2 5-3

5.3 5.3.1 5.3.2

Switching the BioRobot EZ1 DSP on and off Switching on the BioRobot EZ1 DSP Switching off the BioRobot EZ1 DSP

5-4 5-4 5-5

5.4 5.4.1 5.4.2

Opening and closing the workstation door Opening the workstation door Closing the workstation door

5-5 5-6 5-6

5.5 5.5.1 5.5.2

Starting and stopping a protocol run Starting a protocol run Stopping a protocol run

5-6 5-6 5-7

5.6 5.6.1 5.6.2 5.6.3

Setting up the worktable 5-8 Removing and replacing the tray 5-9 Loading the reagent cartridges 5-9 Loading the elution tubes, filter-tips, and sample tubes 5-11

6

Preventive Maintenance

6-1

6.1

Regular maintenance procedure

6-2

6.2

Daily maintenance procedure

6-4

Contents-2

BioRobot EZ1 DSP Workstation User Manual 02/2007

Contents 6.3

Weekly maintenance procedure

6-5

6.4

Reagents for decontamination

6-6

7

Troubleshooting

7-1

7.1

Detecting an error

7-1

7.2

Error codes

7-2

8

Glossary

8-1

Appendix A

A-1

Technical data

A-1

Waste Electrical and Electronic Equipment (WEEE)

A-4

Appendix B

B-1

Warranty statement

B-1

Liability clause

B-2

Index

BioRobot EZ1 DSP Workstation User Manual 02/2007

Index-1

Contents-3

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Contents-4

BioRobot EZ1 DSP Workstation User Manual 02/2007

Safety Information

1

Safety Information This manual contains information and warnings that must be followed by the user to ensure safe operation of the BioRobot EZ1 DSP workstation and to maintain the instrument in a safe condition. Possible hazards that could harm the user or result in damage to the instrument are clearly stated at the appropriate places throughout this manual. The following safety conventions are used throughout this manual. WARNING

The term WARNING is used to inform you about situations that could result in personal injury to you or other persons. Details about these circumstances are given in a box like this one.

CAUTION

The term CAUTION is used to inform you about situations that could result in damage to the instrument or other equipment. Details about these circumstances are given in a box like this one. Before using the instrument, it is essential to read this manual carefully and to pay particular attention to any advice it contains concerning hazards that may arise from use of the instrument. The advice given in this manual is intended to supplement, not supersede, the normal safety requirements prevailing in the user’s country.

BioRobot EZ1 DSP Workstation User Manual 02/2007

1-1

Safety Information

1.1 WARNING

Proper use Risk of personal injury and material damage [W1] Improper use of the BioRobot EZ1 DSP may cause personal injuries or damage to the instrument. The BioRobot EZ1 DSP must only be operated by qualified personnel who have been appropriately trained. Servicing of the BioRobot EZ1 DSP must only be performed by QIAGEN Instrument Service Specialists. Use only QIAGEN® components, otherwise your right to make a claim under the guarantee may be invalidated. Carry out the maintenance regularly in accordance with the operating instructions. QIAGEN charges for repairs that prove to be required due to incorrect maintenance.

CAUTION

Damage to the instrument [C1] Avoid spilling water or chemicals onto the BioRobot EZ1 DSP. Instrument damage caused by water or chemical spillage will void your warranty. In case of an emergency, switch off the BioRobot EZ1 DSP at the power switch on the rear of the instrument and unplug the power cord from the wall power outlet.

CAUTION

1.2

Damage to the instrument [C2] Ensure that the BioRobot EZ1 DSP is switched off before you manually move the mechanical components of the instrument.

Electrical safety To ensure satisfactory and safe operation of the BioRobot EZ1 DSP, it is essential that the line power cord is connected to true electrical earth (ground).

1-2

BioRobot EZ1 DSP Workstation User Manual 02/2007

Safety Information WARNING

Electrical hazard [W2] Any interruption of the protective conductor (earth/ground lead) inside or outside the instrument or disconnection of the protective conductor terminal is likely to make the instrument dangerous. Intentional interruption is prohibited. Lethal voltages inside the instrument When the instrument is connected to line power, terminals may be live, and opening covers or removing parts is likely to expose live parts. When working with the BioRobot EZ1 DSP: „ Make sure the line power cord is connected to a line power outlet that has a protective conductor (earth/ ground). „ Do not attempt to make any internal adjustments or replacements. „ Do not operate the instrument with any covers or parts removed. „ If water or reagent has spilled inside the instrument, switch off the instrument and disconnect it from the line power supply. Call QIAGEN Technical Services. „ Servicing should be carried out only by QIAGEN Instrument Service Specialists. „ If the instrument becomes electrically unsafe for use, make the instrument inoperative and secure it against unauthorized or unintentional operation. Call QIAGEN Technical Services. The instrument is likely to be electrically unsafe when: „ It shows visible damage „ The line power cord shows signs of damage „ It has been stored under unfavorable conditions for a prolonged period „ It has been subjected to severe transport stresses

WARNING

Risk of electric shock Do not touch the BioRobot EZ1 DSP with wet hands.

BioRobot EZ1 DSP Workstation User Manual 02/2007

[W3]

1-3

Safety Information

Voltage rating label The following label appears on the back of the BioRobot EZ1 DSP. Ensure that the voltage rating specified on the label matches the voltage available at the installation site.

1.3

Environment Operating conditions

WARNING

Explosive atmosphere The BioRobot EZ1 DSP is not designed for use in an explosive atmosphere.

[W4]

The BioRobot EZ1 DSP will operate correctly under the following conditions: „ Indoors „ Ambient temperature of 5–40ºC (41–104ºF) „ Ambient relative humidity of <80% at <31ºC (88ºF) (no condensation)

Storage conditions If you intend to store the instrument for a prolonged period of time, unplug the power cord from the wall power outlet. The instrument can be stored at –25ºC to 70ºC (–13ºF to 158ºF).

1-4

BioRobot EZ1 DSP Workstation User Manual 02/2007

Safety Information

1.4

Biological safety Specimens and reagents containing materials from humans should be treated as potentially infectious. Use safe laboratory procedures as outlined in publications such as Biosafety in Microbiological and Biomedical Laboratories, HHS (www.cdc.gov/od/ohs/biosfty/biosfty.htm).

Samples Samples may contain infectious agents. You should be aware of the health hazard presented by such agents and should use, store, and dispose of such samples in accordance with the required safety regulations. WARNING

Samples containing infectious agents [W5] Some samples used with this instrument may contain infectious agents. Handle such samples with the greatest of care and in accordance with the required safety regulations. Always wear safety glasses, two pairs of gloves, and a lab coat. The responsible body (e.g., laboratory manager) must take the necessary precautions to ensure that the surrounding workplace is safe, and that the instrument operators are suitably trained and not exposed to hazardous levels of infectious agents as defined in the applicable Material Safety Data Sheets (MSDSs) or OSHA, ACGIH, or COSHH documents. Venting for fumes and disposal of wastes must be in accordance with all national, state, and local health and safety regulations and laws.

OSHA:

Occupational Safety and Health Administration (United States of America).

ACGIH:

American Conference of Government Industrial Hygienists (United States of America).

COSHH: Control of Substances Hazardous to Health (United Kingdom).

BioRobot EZ1 DSP Workstation User Manual 02/2007

1-5

Safety Information

1.5

Chemicals

WARNING

Hazardous chemicals [W6] Some chemicals used with this instrument may be hazardous or may become hazardous after completion of the protocol run. Always wear safety glasses, gloves, and a lab coat. The responsible body (e.g., laboratory manager) must take the necessary precautions to ensure that the surrounding workplace is safe and that the instrument operators are not exposed to hazardous levels of toxic substances (chemical or biological) as defined in the applicable Material Safety Data Sheets (MSDSs) or OSHA, ACGIH, or COSHH documents. Venting for fumes and disposal of wastes must be in accordance with all national, state, and local health and safety regulations and laws.

OSHA:

Occupational Safety and Health Administration (United States of America).

ACGIH:

American Conference of Government Industrial Hygienists (United States of America).

COSHH: Control of Substances Hazardous to Health (United Kingdom).

Toxic fumes If you work with volatile solvents, toxic substances, etc., you must provide an efficient laboratory ventilation system to remove vapors that may be produced.

1.6

Waste disposal Used labware, such as reagent cartridges and filter-tips, may contain hazardous chemicals or infectious agents from the purification process. Such wastes must be collected and disposed of properly in accordance with the local safety regulations. For disposal of waste electrical and electronic equipment (WEEE), see page A-4.

1-6

BioRobot EZ1 DSP Workstation User Manual 02/2007

Safety Information

1.7

Mechanical hazards The worktable of the BioRobot EZ1 DSP moves during operation of the instrument.

WARNING

Moving parts [W7] Never open the workstation door while the BioRobot EZ1 DSP is operating.

WARNING

Risk of overheating [W8] To ensure proper ventilation, maintain a minimum clearance of 15 cm and 30 cm at the rear and sides of the BioRobot EZ1 DSP, respectively. Slits and openings which ensure the ventilation of the BioRobot EZ1 DSP must not be covered.

1.8

Heat hazard The BioRobot EZ1 DSP worktable contains a heating system.

WARNING

Hot surface [W9] The heating system can reach high temperatures. Avoid touching it when it is hot.

BioRobot EZ1 DSP Workstation User Manual 02/2007

1-7

Safety Information

1.9 Symbol

1-8

Symbols on the BioRobot EZ1 DSP Location

Description

Heating system

Heat hazard - the temperature of the heating system can reach up to 95ºC

Near the tip rack

Biohazard - the tip rack may be contaminated with biohazardous material and must be handled with gloves

Type plate on the back of the instrument

CE mark for Europe

Type plate on the back of the instrument

UL listing mark for Canada and the USA

Type plate on the back of the instrument

WEEE mark for Europe

Type plate on the back of the instrument

In vitro diagnostic medical device

BioRobot EZ1 DSP Workstation User Manual 02/2007

Safety Information Symbol

SN

1.10 Symbol

Location

Description

Type plate on the back of the instrument

Serial number

Type plate on the back of the instrument

Legal manufacturer

Additional symbols in this user manual Location

Description

Front cover

Catalog number

Front cover

Handbook

BioRobot EZ1 DSP Workstation User Manual 02/2007

1-9

Safety Information Symbol on the Heating System

Symbol near the Tip Rack

1-10

BioRobot EZ1 DSP Workstation User Manual 02/2007

Introduction

2

Introduction Thank you for choosing the QIAGEN BioRobot EZ1 DSP workstation. We are confident it will become an integral part of your laboratory. Before using the instrument, it is essential to read this manual carefully and to pay particular attention to any advice it contains concerning hazards that may arise from use of this instrument.

2.1

About this user manual This manual provides information about the BioRobot EZ1 DSP. It guides you systematically through the following sections: 1. Safety Information 2. Introduction 3. BioRobot EZ1 DSP - General Description 4. Installation Procedures 5. General Operation 6. Preventive Maintenance 7. Troubleshooting 8. Glossary Appendices The Appendices contain the following: „ Contact information for technical assistance „ Technical data „ Warranty terms

2.2

General information

2.2.1

Technical assistance At QIAGEN we pride ourselves on the quality and availability of our technical support. Our Technical Service Departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of

BioRobot EZ1 DSP Workstation User Manual 02/2007

2-1

Introduction QIAGEN products. If you have any questions regarding the BioRobot EZ1 DSP or QIAGEN products in general, please do not hesitate to contact us. QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. This information is helpful to other scientists as well as to the researchers at QIAGEN. We therefore encourage you to contact us if you have any suggestions about product performance or new applications and techniques. For technical assistance and more information please call one of the QIAGEN Technical Service Departments or local distributors (see back cover).

2.2.2

Policy statement It is the policy of QIAGEN to improve products as new techniques and components become available. QIAGEN reserves the right to change specifications at any time. In an effort to produce useful and appropriate documentation, we appreciate your comments on this publication. Please contact QIAGEN Technical Services.

2.2.3

Version management This document is the BioRobot EZ1 DSP Workstation User Manual version 1, revision R1.

2.3

Intended use of the BioRobot EZ1 DSP The BioRobot EZ1 DSP workstation is designed to perform automated purification of nucleic acids for in vitro diagnostic purposes. The system is intended for use by professional users, such as technicians and physicians trained in molecular biological techniques and the operation of the BioRobot EZ1 DSP. Any diagnostic results that are generated using the sample preparation procedure in conjunction with any downstream diagnostic assay must be interpreted in conjunction with other clinical or laboratory findings.

2-2

BioRobot EZ1 DSP Workstation User Manual 02/2007

Introduction

2.3.1

Requirements for BioRobot EZ1 DSP users This table covers the general level of competence and training necessary for transportation, installation, use, maintenance, and servicing of the BioRobot EZ1 DSP.

Type of task

Personnel

Training and experience

Transportation

No special requirements

No special requirements

Installation

Laboratory technicians or equivalent

Appropriately trained and experienced personnel familiar with use of computers and automation in general

Routine use (running protocols)

Laboratory technicians or equivalent

Appropriately trained and experienced personnel familiar with use of computers and automation in general

Preventive maintenance

Laboratory technicians or equivalent

Appropriately trained and experienced personnel familiar with use of computers and automation in general

Servicing and annual preventive maintenance

QIAGEN Instrument Service Specialists only

BioRobot EZ1 DSP Workstation User Manual 02/2007

2-3

Introduction This page intentionally left blank

2-4

BioRobot EZ1 DSP Workstation User Manual 02/2007