User Manual
121 Pages
Preview
Page 1
User Manual
January 2003
Trademarks Patented or patent-pending technology and/or registered or registration-pending trademarks of the QIAGEN Group: QIAGEN®, QIAsoft™, BioRobot®, MagAttract®. Adobe is a registered trademark of Adobe Systems Incorporated. Celeron and Pentium are registered trademarks of Intel Corporation. HEPA is a registered trademark of HEPA Corporation. Microsoft and Windows are registered trademarks of Microsoft Corporation. Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law. QIAGEN Robotic Systems are not available in all countries; please inquire. © 2003 QIAGEN, all rights reserved.
1023287 01/2003
QIAGEN Worldwide QIAGEN Companies Australia
QIAGEN Pty Ltd ABN 75 072 382 944
PO Box 25 • Clifton Hill • Victoria 3068 Orders 03-9489-3666 • Fax 03-9489-3888 • Technical 1-800-243-066
Canada
QIAGEN Inc.
2800 Argentia Road • Unit 7 • Mississauga • Ontario • L5N 8L2 Orders 800-572-9613 • Fax 800-713-5951 • Technical 800-DNA-PREP (800-362-7737)
France
QIAGEN S.A.
3 avenue du Canada • LP 809 • 91974 COURTABOEUF CEDEX Orders 01-60-920-920 • Fax 01-60-920-925 • Technical 01-60-920-930
Germany
QIAGEN GmbH
Max-Volmer-Straße 4 • 40724 Hilden Orders 02103-29-12000 • Fax 02103-29-22000 • Technical 02103-29-12400
Italy
QIAGEN S.p.A.
Via Grosio, 10/10 • 20151 Milano Orders 02-33430411 • Fax 02-33430426 • Technical 02-33430414
Japan www.qiagen.co.jp
QIAGEN K.K.
Forefront Tower II • 13-1, Kachidoki 3 Chome • Chuo-ku, Tokyo 104-0054 Telephone 03-5547-0811 • Fax 03-5547-0818 • Technical 03-5547-0811
Switzerland
QIAGEN AG
Auf dem Wolf 39 • 4052 Basel Orders 061-319-30-30 • Fax 061-319-30-33 • Technical 061-319-30-31
UK and Ireland
QIAGEN Ltd.
Boundary Court • Gatwick Road • Crawley • West Sussex, RH10 9AX Orders 01293-422-911 • Fax 01293-422-922 • Technical 01293-422-999
USA
QIAGEN Inc.
28159 Avenue Stanford • Valencia • CA 91355 Orders 800-426-8157 • Fax 800-718-2056 • Technical 800-DNA-PREP (800-362-7737)
www.qiagen.com
QIAGEN Distributors Please see the last page for contact information for your local QIAGEN distributor.
Contents
Contents 1
Safety Information ...1-1
1.1
Proper use ...1-2
1.2
Electrical safety ...1-3
1.3
Environment ...1-4
1.4
Biological safety ...1-5
1.5
Chemicals ...1-6
1.6
Waste disposal...1-6
1.7
Mechanical hazards ...1-7
1.8
Heat hazard ...1-7
1.9
Translations of warnings and cautions ...1-8
1.10
Symbols on the BioRobot M48 ...1-20
2
Introduction ...2-1
2.1
About this manual...2-1
2.2 2.2.1 2.2.2 2.2.3
General information...2-2 Policy statement ...2-2 Software for operating the BioRobot M48 ...2-2 Version management ...2-2
2.3 2.3.1
Intended use of the BioRobot M48...2-3 Requirements for BioRobot M48 users...2-4
3
BioRobot M48 - General Description ...3-1
3.1
External features of the BioRobot M48 ...3-2
3.2 3.2.1
Internal features of the BioRobot M48...3-5 Worktable...3-5
QIAGEN BioRobot M48 01/2003
Contents-1
Contents 3.2.2
Pipettor head ... 3-14
4
Installation Procedures...4-1
4.1
Requirements ... 4-1
4.2
AC power connection... 4-2
4.3
Hardware installation... 4-4
4.4
Software installation... 4-6
5
General Operation...5-1
5.1
Overview ... 5-1
5.2 5.2.1 5.2.2 5.2.3 5.2.4 5.2.5
Starting up and shutting down the BioRobot M48... 5-2 Starting up the BioRobot M48... 5-2 Opening the QIAsoft M software ... 5-3 Closing the QIAsoft M software ... 5-4 Error log window ... 5-4 Shutting down the BioRobot M48... 5-5
5.3 5.3.1 5.3.2
Main features of the QIAsoft M software... 5-5 Menu bar... 5-5 Protocol selection box ... 5-8
5.4 5.4.1 5.4.2
Running a protocol ... 5-8 Selecting a protocol ... 5-9 Checking the tip guard insert and waste chute ... 5-11 Loading the disposable tips ... 5-12 Loading the reagents ... 5-13 Importing and exporting a memo ... 5-14 Loading the sample prep plates... 5-15 Loading Heat/Cool Block 1... 5-16 Loading Heat/Cool Block 2... 5-17
5.4.3 5.4.4 5.4.5 5.4.6 5.4.7 5.4.8
Contents-2
QIAGEN BioRobot M48 01/2003
Contents 5.4.9 5.4.10 5.4.11 5.4.12 5.4.13 6
Loading the samples ...5-19 Confirming the worktable layout ...5-22 Checking the status of the protocol run...5-23 Viewing the results of the protocol run ...5-25 Sterilizing the BioRobot M48 ...5-27
Preventive Maintenance ...6-1
6.1 6.1.1
Daily maintenance procedure ...6-2 Cleaning the tip guard insert...6-4
6.2 6.2.1 6.2.2
Weekly maintenance procedure ...6-4 Greasing the O-rings ...6-5 Replacing the O-rings...6-6
6.3 6.3.1
Monthly maintenance procedure ...6-7 Performing an O-ring leakage test ...6-7
7
Troubleshooting...7-1
7.1
General troubleshooting...7-1
7.2 7.2.1 7.2.2 7.2.3
Errors displayed in the QIAsoft M software ...7-2 Recording an error ...7-2 Types of error log codes ...7-3 Common error codes ...7-8
8
Glossary...8-1
Appendix A... A-1 Technical assistance...A-1 Appendix B ...B-1 Technical data ... B-1
QIAGEN BioRobot M48 01/2003
Contents-3
Contents Appendix C... C-1 Warranty statement ... C-1 Liability clause ... C-2 Index ... Index-1
Contents-4
QIAGEN BioRobot M48 01/2003
Safety Information
1
Safety Information This manual contains information and warnings that must be followed by the user to ensure safe operation of the BioRobot® M48 workstation and to maintain the instrument in a safe condition. Possible hazards that could harm the user or result in damage to the instrument are clearly stated at the appropriate places throughout this manual. The following safety conventions are used throughout this manual.
WARNING
The term WARNING is used to inform you about situations that could result in personal injury to you or other persons. Details about these circumstances are given in a box like this one.
CAUTION
The term CAUTION is used to inform you about situations that could result in damage to the instrument or other equipment. Details about these circumstances are given in a box like this one. Translations of the warnings and cautions used in this manual are given at the end of this section. Each translated warning or caution has a reference number in square brackets at the top right of its box (e.g., [W1], [C1]). Before using the instrument, it is essential to read this manual carefully and to pay particular attention to any advice it contains concerning hazards that may arise from use of the instrument. The advice given in this manual is intended to supplement, not supersede, the normal safety requirements prevailing in the user’s country.
QIAGEN BioRobot M48 01/2003
1-1
Safety Information
1.1 WARNING
Proper use Risk of personal injury and material damage [W1] Improper use of the BioRobot M48 may cause personal injuries or damage to the instrument. The BioRobot M48 should only be operated by qualified personnel who have been appropriately trained. Servicing of the BioRobot M48 should only be performed by QIAGEN Instrument Service Specialists. Use only QIAGEN® components, otherwise your right to make a claim under the guarantee may be invalidated. Carry out the maintenance regularly in accordance with the operating instructions. QIAGEN charges for repairs that prove to be required due to incorrect maintenance.
CAUTION
Damage to the instrument [C1] Avoid spilling water or chemicals onto the BioRobot M48. Instrument damage caused by water or chemical spillage will void your Warranty. In case of an emergency, switch off the BioRobot M48 at the power switch on the left side of the instrument and unplug the power cord from the wall power outlet.
1-2
QIAGEN BioRobot M48 01/2003
Safety Information
1.2
Electrical safety To ensure satisfactory and safe operation of the BioRobot M48, it is essential that the line power cord is connected to true electrical earth (ground).
WARNING
Electrical hazard [W2] Any interruption of the protective conductor (earth/ground lead) inside or outside the instrument or disconnection of the protective conductor terminal is likely to make the instrument dangerous. Intentional interruption is prohibited. Lethal voltages inside the instrument When the instrument is connected to line power, terminals may be live, and opening covers or removing parts is likely to expose live parts. When working with the BioRobot M48: • Make sure the line power cord is connected to a line power outlet that has a protective conductor (earth/ ground). • Do not attempt to make any internal adjustments or replacements. • Do not operate the instrument with any covers or parts removed. • If water or reagent has spilled inside the instrument, switch off the instrument and disconnect it from the line power supply. Call QIAGEN Technical Services. • Servicing should be carried out only by QIAGEN Instrument Service Specialists. • If the instrument becomes electrically unsafe for use, make the instrument inoperative and secure it against unauthorized or unintentional operation. Call QIAGEN Technical Services.
QIAGEN BioRobot M48 01/2003
1-3
Safety Information The instrument is likely to be electrically unsafe when: - It shows visible damage - The line power cord shows signs of damage - It has been stored under unfavorable conditions for a prolonged period - It has been subjected to severe transport stresses WARNING
1.3
Risk of electric shock [W3] The panel on the left side of the BioRobot M48 contains connectors for the power cord and the computer cables. Do not touch this panel with wet hands.
Environment Operating conditions
WARNING
Explosive atmosphere [W4] The BioRobot M48 is not designed for use in an explosive atmosphere. The BioRobot M48 will operate correctly under the following conditions: • Indoors • Ambient temperature of 15–30ºC (59–86ºF) • Ambient relative humidity of <80% at <30ºC (86ºF)
Storage conditions If you intend to store the instrument for a prolonged period of time, unplug the power cord from the wall power outlet. The instrument can be stored at –40ºC to 65ºC (–40ºF to 149ºF).
1-4
QIAGEN BioRobot M48 01/2003
Safety Information
1.4
Biological safety Specimens and reagents containing materials from humans should be treated as potentially infectious. Use safe laboratory procedures as outlined in publications such as Biosafety in Microbiological and Biomedical Laboratories, HHS (www.cdc.gov/od/ohs/biosfty/biosfty.htm).
Samples Samples may contain infectious agents. You should be aware of the health hazard presented by such agents and should use, store, and dispose of such samples in accordance with the required safety regulations. WARNING
Samples containing infectious agents [W5] Some samples used with this instrument may contain infectious agents. Handle such samples with the greatest of care and in accordance with the required safety regulations. Always wear safety glasses, two pairs of gloves, and a lab coat. The responsible body (e.g., laboratory manager) must take the necessary precautions to ensure that the surrounding workplace is safe, and that the instrument operators are suitably trained and not exposed to hazardous levels of infectious agents as defined in the applicable Material Safety Data Sheets (MSDSs) or OSHA, ACGIH, or COSHH documents. Venting for fumes and disposal of wastes must be in accordance with all national, state, and local health and safety regulations and laws.
OSHA:
Occupational Safety and Health Administration (United States of America)
ACGIH:
American Conference of Government Industrial Hygienists (United States of America)
COSHH: Control of Substances Hazardous to Health (United Kingdom)
QIAGEN BioRobot M48 01/2003
1-5
Safety Information
1.5
Chemicals
WARNING
Hazardous chemicals [W6] Some chemicals used with this instrument may be hazardous or may become hazardous after completion of the protocol run. Always wear safety glasses, gloves, and a lab coat. The responsible body (e.g., laboratory manager) must take the necessary precautions to ensure that the surrounding workplace is safe and that the instrument operators are not exposed to hazardous levels of toxic substances (chemical or biological) as defined in the applicable Material Safety Data Sheets (MSDSs) or OSHA, ACGIH, or COSHH documents. Venting for fumes and disposal of wastes must be in accordance with all national, state, and local health and safety regulations and laws.
OSHA:
Occupational Safety and Health Administration (United States of America)
ACGIH:
American Conference of Government Industrial Hygienists (United States of America)
COSHH: Control of Substances Hazardous to Health (United Kingdom)
Toxic fumes If you work with volatile solvents, toxic substances, and so on, you must provide an efficient laboratory ventilation system to remove vapors that may be produced.
1.6
Waste disposal Used labware, such as sample prep plates and disposable tips, may contain hazardous chemicals or infectious agents from the purification process. Such wastes must be collected and disposed of properly in accordance with the local safety regulations.
1-6
QIAGEN BioRobot M48 01/2003
Safety Information
1.7
Mechanical hazards To protect users, the pipettor head of the BioRobot M48 will not move until the workstation door is closed.
WARNING
Moving parts [W7] To avoid contact with the moving pipettor head during operation of the BioRobot M48, do not insert your hands and fingers through the waste chute at the front of the instrument. Ventilation inlets are located on both sides of the instrument and contain dust filters. A fan blows out air through ventilation outlets located at the bottom or on the opposite side of the instrument. Another 2 fans blow out air from the worktable through a HEPA® filter.
WARNING
1.8
Risk of overheating [W8] To ensure proper ventilation, maintain a minimum clearance of 5 cm and 15 cm at the rear and left of the BioRobot M48, respectively. Slits and openings which ensure the ventilation of the BioRobot M48 must not be covered.
Heat hazard The right-hand side of the BioRobot M48 worktable contains 2 cooling and heating systems.
WARNING
Hot surface [W9] The cooling and heating systems can reach high temperatures. Avoid touching them when they are hot.
QIAGEN BioRobot M48 01/2003
1-7
Safety Information
1.9
Translations of warnings and cautions This subsection contains translations of the warnings and cautions used in this manual. Each warning or caution has a reference number in square brackets at the top right of its box.
WARNING
The term WARNING is used to inform you about situations that could result in personal injury to you or other persons. Details about these circumstances are given in a box like this one.
DE
WARNING (WARNUNG) WARNUNG weist auf Situationen und Umstände hin, die zu einer Verletzung des Benutzers oder anderer Personen führen können. Nähere Angaben zu der Art der Gefährdung und der Vermeidung solcher Situationen werden in einem Textfeld wie diesem neben der Warnung gemacht.
FR
WARNING (DANGER) La formule WARNING (DANGER) est utilisée pour avertir des situations pouvant occasionner des dommages corporels à l’utilisateur ou à d’autres personnes. Les détails sur ces circonstances sont données dans un encadré semblable à celui-ci.
1-8
QIAGEN BioRobot M48 01/2003
Safety Information WARNING
Risk of electric shock [W3] The panel on the left side of the BioRobot M48 contains connectors for the power cord and the computer cables. Do not touch this panel with wet hands.
DE
Gefährdung durch Elektrizität Auf der linken Seite des BioRobot M48 befindet sich das Feld mit den Anschlüssen und Buchsen für die Stromversorgung und die Computerkabel. Dieses Anschlussfeld darf nicht mit nassen Händen berührt werden.
FR
Risque de l’électrocution Le panneau avec les connexions pour le câble d’alimentation et les câbles de l’ordinateur se trouve du côté gauche du BioRobot M48. Ne pas y toucher avec des mains mouillées.
WARNING
Explosive atmosphere [W4] The BioRobot M48 is not designed for use in an explosive atmosphere.
DE
Explosionsfähige Atmosphären Der BioRobot M48 darf nicht in explosionsfähigen Atmosphären betrieben werden.
FR
Atmosphère explosive Le BioRobot M48 n’est pas conçue pour fonctionner dans une atmosphère explosive.
QIAGEN BioRobot M48 01/2003
1-11
Safety Information WARNING
Moving parts [W7] To avoid contact with the moving pipettor head during operation of the BioRobot M48, do not insert your hands and fingers through the waste chute at the front of the instrument.
DE
Bewegliche Geräteteile Um den Kontakt mit dem sich bewegenden Pipettierkopf während des Betriebs des BioRobot M48 auszuschließen, dürfen Hände oder Finger nicht in die Öffnung der Pipettenspitzen-Abwurfstation an der Vorderseite des Geräts gesteckt werden.
FR
Eléments mobiles Afin d’éviter le contact avec le bras pipetteur mobile, ne pas placer les mains ou doigts dans le canal d’évacuation de cônes à l’avant de l’appareil pendant que le BioRobot M48 est en marche.
1-16
QIAGEN BioRobot M48 01/2003
Safety Information Symbol
Location
Language
Description
EN
CE marking
DE
CE-Zeichen
FR
Marquage CE
EN
UL listing mark for Canada and the USA
DE
Symbol der UL-Zertifizierung in Kanada und den USA
FR
Certifié UL au Canada et aux Etats Unis
Location of Symbols on the BioRobot M48
1-22
QIAGEN BioRobot M48 01/2003
Introduction
2
Introduction Thank you for choosing the QIAGEN BioRobot M48 molecular biology workstation. We are confident it will become an integral part of your laboratory. Before using the instrument, it is essential to read this manual carefully and to pay particular attention to any advice it contains concerning hazards that may arise from use of this instrument.
2.1
About this manual This manual provides information about the BioRobot M48. It guides you systematically through the following sections: 1. Safety Information 2. Introduction 3. BioRobot M48 - General Description 4. Installation Procedures 5. General Operation 6. Preventive Maintenance 7. Troubleshooting 8. Glossary Appendices The Appendices contain the following: • Contact information for technical assistance • Technical data • Warranty terms
QIAGEN BioRobot M48 01/2003
2-1
Introduction
2.2
General information
2.2.1
Policy statement It is the policy of QIAGEN to improve products as new techniques and components become available. QIAGEN reserves the right to change specifications at any time. In an effort to produce useful and appropriate documentation, we appreciate your comments on this publication. Please contact QIAGEN Technical Services.
2.2.2
Software for operating the BioRobot M48 The QIAsoft™ M Operating System allows operation of the BioRobot M48. This software runs under the Microsoft® Windows® 2000 operating system, and uses typical Windows features such as menus, command buttons, and dialog boxes. You should be familiar with the Windows operating environment, especially the file management system. For more information, consult the Microsoft user guides.
Controlling the mouse The following terms for controlling the mouse are used in this manual.
2.2.3
Action
Description
Click
Click the left mouse button.
Select “XXX/xxx”
In the menu bar, click the “XXX” menu and select “xxx” from the drop-down list. An example is “Manual Operation/Sterilization”.
Version management This document is the BioRobot M48 User Manual version 1.0.
2-2
QIAGEN BioRobot M48 01/2003
Introduction
2.3
Intended use of the BioRobot M48 The BioRobot M48 is designed to perform fully automated purification of nucleic acids using MagAttract® magnetic particles. The BioRobot M48 is intended as a microtiter diluting and dispensing device. No claim or representation is intended for its use in identifying any specific organism or for a specific clinical use (diagnostic, prognostic, therapeutic, or blood banking). It is the user’s responsibility to validate the performance of the BioRobot M48 for any particular use, since its performance characteristics have not been validated for any specific organism. The BioRobot M48 may be used in clinical diagnostic laboratory systems after the laboratory has validated their complete system as required by CLIA ‘88 regulations in the U.S. or equivalents in other countries.
QIAGEN BioRobot M48 01/2003
2-3