QIAxcel DNA Handbook 4th Edition April 2013

QIAGEN Sample and Assay Technologies QIAGEN is the leading provider of innovative sample and assay technologies, enabling the isolation and detection of contents of any biological sample. Our advanced, high-quality products and services ensure success from sample to result. QIAGEN sets standards in:



Purification of DNA, RNA, and proteins



Nucleic acid and protein assays



microRNA research and RNAi



Automation of sample and assay technologies

Our mission is to enable you to achieve outstanding success and breakthroughs. For more information, visit www.qiagen.com.

Contents Kit Contents

5

Unpacking the QIAxcel Gel Cartridge

6

Storage

6

Intended Use

7

Safety Information

8

Quality Control

8

Introduction

9

Principle and procedure

9

Equipment and Reagents to Be Supplied by User

11

Important Notes

12

Preparing the QIAxcel gel cartridge and buffer tray

12

Sample preparation recommendations

18

Marker selection

18

Method selection

20

Protocols  

Determination of DNA Fragment Sizes using QIAxcel ScreenGel Software

21

Determination of DNA Fragment Sizes using BioCalculator Software

24

Troubleshooting Guide

28

Appendix A: Creating a Process Profile in QIAxcel ScreenGel Software 29 Appendix B: Data Analysis

30

Aligning the gel image with BioCalculator software

30

Creating a DNA size marker reference table

32

Determination of DNA length

37

Appendix C: QIAxcel Methods

38

QIAxcel High Resolution methods

38

QIAxcel DNA Screening methods

40

QIAxcel DNA Fast Analysis methods

43

Appendix D: Removing Gel from Blocked Capillaries

44

Standard gel-droplet test

44

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Gel-droplet test with hot water

46

Gel-droplet test using the QX Cartridge Prep Station

47

Performing a signal check

49

References

50

Ordering Information

51

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Kit Contents QIAxcel DNA High Resolution Kit

(1200)

Catalog no.

929002

Number of assays

12 x 100

QIAxcel DNA High Resolution Cartridge (with smart key)

1

QX Separation Buffer*

40 ml

QX Wash Buffer*

40 ml

QX Mineral Oil

50 ml

QX DNA Dilution Buffer

15 ml

QX Intensity Calibration Marker

600 µl

QX 0.2 ml 12-Tube Strips

2

QX Colored 0.2 ml 12-Tube Strips

2

Handbook

1

* Contains sodium azide as a preservative.

QIAxcel DNA Screening Kit

(2400)

Catalog no.

929004

Number of assays

12 x 200

QIAxcel DNA Screening Cartridge (with smart key)

1

QX Separation Buffer*

40 ml

QX Wash Buffer*

40 ml

QX Mineral Oil

50 ml

QX DNA Dilution Buffer

15 ml

QX Intensity Calibration Marker

600 µl

QX 0.2 ml 12-Tube Strips

2

QX Colored 0.2 ml 12-Tube Strips

2

Handbook

1

* Contains sodium azide as a preservative.

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QIAxcel DNA Fast Analysis Kit

(3000)

Catalog no.

929008

Number of assays

12 x 250

QIAxcel DNA Fast Analysis Cartridge (with smart key)

1

QX FA Separation Buffer*

40 ml

QX Wash Buffer

40 ml

QX Mineral Oil

50 ml

QX Intensity Calibration Marker

600 µl

QX 0.2 ml 12-Tube Strips

2

QX Colored 0.2 ml 12-Tube Strips

2

QX DNA Size Marker 50 bp – 1.5 kb

100 µl

QX Alignment Marker 15 bp/3 kb

1.5 ml

Handbook

1

The QIAxcel DNA Fast Analysis Kit is not suited for concentration determination. * Contains sodium azide as a preservative.

Unpacking the QIAxcel Gel Cartridge For optimal performance, store the QIAxcel gel cartridge at 2–8°C until the first use. Prior to use, the QIAxcel gel cartridge should be placed into the QX Cartridge Stand and protected with the cover whenever exposed to light, or stored latched in the instrument in the “Park Position” with buffer in the buffer tray, and allowed to stand for at least 20 minutes (for more information, see “Preparing the QIAxcel gel cartridge and buffer tray”, page 12).

Storage All components of the QIAxcel DNA High Resolution Kit, and the QIAxcel DNA Screening Kit, except for the gel cartridge and the QX Intensity Calibration Marker, can be stored dry at room temperature (15–25°C). The QIAxcel gel cartridge and the marker should be stored at 2–8°C upon arrival. All components of the QIAxcel DNA Fast Analysis Kit, except for the gel cartridge and the markers, can be stored dry at room temperature. The QIAxcel gel cartridge and the markers should be stored at 2–8°C upon arrival.

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QIAxcel DNA Handbook 04/2013

Store QIAxcel DNA gel cartridges at 2–8°C until the first use. If the QIAxcel gel cartridge is not being used on a daily basis, close the purge port with the purge port seal, return the QIAxcel DNA gel cartridge to the blister package, inserting the capillary tips into the soft gel, and store at 2–8°C in an upright position (see orientation label on blister package). Note: Storing the QIAxcel DNA gel cartridge below 2°C can severely damage the cartridge. Prior to use, the QIAxcel DNA gel cartridge should be placed into the QX Cartridge Stand and protected with the cover whenever exposed to light, or stored latched in the instrument in the “Park Position” with buffer in the buffer tray, and allowed to stand for at least 20 minutes. If the QIAxcel DNA gel cartridge is being used on a daily basis, store the cartridge latched in the QIAxcel instrument in the “Park Position” (refer to Section 5.3 of the QIAxcel User Manual or the QIAxcel Advanced User Manual). Note: The QIAxcel instrument must be left on if the cartridge is stored in the “Park Position” and the cartridge must be latched. Do not switch the QIAxcel instrument off. If more than one QIAxcel DNA gel cartridge is being used on a daily basis, store the second cartridge in the QX Cartridge Stand in the dark or protected with the cover. Make sure that the cartridge stand reservoir is filled with wash buffer and covered with mineral oil (refer to Section 5.1 of the QIAxcel User Manual or Section 5.2.5 the QIAxcel Advanced User Manual). Alternatively, close the purge port with the purge port seal, return the QIAxcel DNA gel cartridge to the blister package, inserting the capillary tips into the soft gel, and store at 2–8°C in an upright position (see orientation label on blister package). The QIAxcel DNA gel cartridge can be stored in this manner until the expiration date indicated on the kit label.

Intended Use QIAxcel DNA Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease. All due care and attention should be exercised in the handling of the products. We recommend all users of QIAGEN® products to adhere to the NIH guidelines that have been developed for recombinant DNA experiments, or to other applicable guidelines.

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Safety Information When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. For more information, please consult the appropriate safety data sheets (SDSs). These are available online in convenient and compact PDF format at www.qiagen.com/safety where you can find, view, and print the SDS for each QIAGEN kit and kit component. 24-hour emergency information Chemical emergency or accident assistance is available 24 hours a day from: CHEMTREC USA & Canada  Tel: 1-800-424-9300 Outside USA & Canada  Tel: +1-703-527-3887 (collect calls accepted)

Quality Control In accordance with QIAGEN’s ISO-certified Quality Management System, each lot of QIAxcel DNA High Resolution Kit, QIAxcel DNA Screening Kit, and QIAxcel DNA Fast Analysis Kit is tested against predetermined specifications to ensure consistent product quality.

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QIAxcel DNA Handbook 04/2013

Introduction QIAxcel instruments, in combination with the QIAxcel DNA High Resolution Kit, the QIAxcel DNA Screening Kit, and the QIAxcel DNA Fast Analysis Kit, provide fully automated separation of DNA fragments according to size, with processing of up to 96 samples per run. QIAxcel technology, based on capillary electrophoresis using QIAxcel gel cartridges, provides unmatched resolution, speed, and throughput. QIAxcel gel cartridges are reusable, allowing multiple runs of 12 samples to be performed (up to 100 runs with the QIAxcel DNA High Resolution Kit, 200 runs with the QIAxcel DNA Screening Kit, and 250 runs with the QIAxcel DNA Fast Analysis Kit). QIAxcel instruments are preinstalled with methods suitable for most applications. In addition, customized methods can also be created - contact QIAGEN Technical Services for more details. QIAxcel ScreenGel® software supplied with the QIAxcel Advanced instrument provides both electropherogram and gel images of nucleic acid separation. Kits for separation and quantification of RNA and protein are also available (see ordering information, page 51).

Principle and procedure The QIAxcel Advanced system uses capillary gel electrophoresis to enable fast separation of nucleic acids based on size. Unlike traditional agarose gel electrophoresis, separation is performed in a capillary of a precast gel cartridge. Each sample is automatically loaded into an individual capillary (according to voltage and time parameters) and voltage is applied. The negatively charged nucleic acid molecules migrate through the capillary to the positively charged end (Figure 1, page 10). As with agarose gel electrophoresis, low-molecular-weight molecules migrate faster than high-molecular-weight molecules. As the molecules migrate through the capillary, they pass a detector that detects and measures the fluorescent signal. A photomultiplier detector converts the emission signal into electronic data, which are then transferred to the computer for further processing using QIAxcel ScreenGel software or BioCalculator software. After processing, the data are displayed as an electropherogram or gel image.

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The QIAxcel Advanced system offers a number of advantages over traditional agarose gel electrophoresis, including:



Higher detection sensitivity



Less sample wastage (minimal sample input volumes)



Improved fragment resolution



Fast analysis of up to 96 samples



Automated loading and analysis

Figure 1. Sample separation process using the QIAxcel Advanced system. Nucleic acid molecules are separated according to size by applying a voltage to a gel-filled capillary. A photomultiplier detector in the instrument detects the staining dye molecules as they migrate towards the positively charged end of the capillary. The data are converted to an electropherogram and a gel image by the QIAxcel ScreenGel software or the BioCalculator software (not shown).

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QIAxcel DNA Handbook 04/2013

Equipment and Reagents to Be Supplied by User When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. For more information, consult the appropriate safety data sheets (SDSs), available from the product supplier. For all users



Pipets and pipet tips



12-tube strips (e.g., QX 0.2 ml 12-Tube Strip, cat. no. 929703) or 96-well plates



Centrifuge with rotor suitable for 0.2 ml strips or 96-well plates, such as the Centrifuge 4-16 or Centrifuge 4-16K (for ordering information, see www.qiagen.com)



QX Alignment Marker (see “Marker selection”, page 18)* †



QX DNA Size Marker (see “Marker selection”, page 18)*†



Optional: QX DNA Dilution Buffer (cat. no. 929601) may be required to fill empty wells

For QIAxcel users



QIAxcel instrument and BioCalculator Software or QIAxcel ScreenGel software version 1.1.0 or higher (cat. no. 9021163)

For QIAxcel Advanced users



QIAxcel Advanced instrument (cat. no. 9001941) and QIAxcel ScreenGel software version 1.1.0 or higher (license included with cat. no. 9001941)

* For recommended combinations of QX Alignment Marker and QX DNA Size Marker, refer to Table 2, page 20. † QX Alignment Marker 15 bp/3 kb and QX DNA Size Marker 50 bp – 1.5 kb are supplied with the QIAxcel DNA Fast Analysis Kit.

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Important Notes Preparing the QIAxcel gel cartridge and buffer tray This procedure describes how to prepare the QIAxcel DNA High Resolution Cartridge, QIAxcel DNA Screening Cartridge, or QIAxcel DNA Fast Analysis Cartridge and buffer tray prior to DNA analysis. Important points before starting



The volume of buffer supplied is sufficient for a defined number of sample runs (see Table 1). If required, additional buffers can be purchased separately (see ordering information, page 53).

Table 1. Number of runs possible using the supplied buffer volumes Kit

Number of runs

Samples per run

QIAxcel DNA High Resolution Kit

100

12

QIAxcel DNA Screening Kit

200

12

QIAxcel DNA Fast Analysis Kit

250

12



The 0.2 ml 12-tube strips containing QX Alignment Marker and QX Intensity Calibration Marker (if required) should fit loosely in the MARKER1 and MARKER2 positions (see steps 12 and 19).



QX Alignment Markers should be replaced every 50 runs or 3 days, whichever comes first. Additional markers and buffers may need to be purchased (see ordering information, page 53).



When not in use, the 12-tube strip containing QX Alignment Marker should be stored at –20°C.



For optimal performance, store the QIAxcel gel cartridge at 2–8°C until required for use. Prior to use, the QIAxcel gel cartridge should be placed into the QX Cartridge Stand and protected with the cover, or stored latched in the instrument in the “Park Position” with buffer in the buffer tray, and allowed to stand for at least 20 minutes.

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QIAxcel DNA Handbook 04/2013

Things to do before starting



If prepared in advance, the 12-tube strip containing QX Alignment Marker should be equilibrated to room temperature (20–25ºC) and centrifuged briefly before use.



If the QIAxcel DNA gel cartridge is being used for the first time, intensity calibration should be performed (refer to “Intensity calibration”, page 16, and Section 5.4 of the QIAxcel User Manual or Section 6.5.1 of the QIAxcel Advanced User Manual). This is not necessary if the QIAxcel DNA gel cartridge has already been calibrated, unless it is being used on a different QIAxcel or QIAxcel Advanced instrument or a different computer is used to operate the instrument. If a different computer is being used to operate the QIAxcel or QIAxcel Advanced instrument, the calibration log file must be transferred to the new computer so that calibration does not need to be performed again.

Unpacking and preparing the QIAxcel gel cartridge For optimal performance, store the QIAxcel DNA gel cartridge at 2–8°C until required for use. Prior to use, the QIAxcel DNA gel cartridge should be placed into the QX Cartridge Stand protected with the cover, or stored latched in the instrument in the “Park Position” with buffer in the buffer tray, and allowed to stand for at least 20 minutes. 1. Remove all buffer bottles from the kit box. 2. Add 10 ml QX Wash Buffer to both reservoirs of the QX Cartridge Stand (provided with QIAxcel instruments) and cover with 2 ml mineral oil (supplied). 3. Remove the QIAxcel DNA gel cartridge from its packaging and carefully wipe off any soft gel debris from the capillary tips using a soft tissue. 4. Remove the purge cap seal from the back of the QIAxcel DNA gel cartridge and place the gel cartridge in the QX Cartridge Stand. Retain the purge port seal in case you need to store the QIAxcel DNA gel cartridge. Note: Use a soft tissue to wipe off any gel that may have leaked from the purge port. Note: Ensure that the capillary tips are submerged in QX Wash Buffer. 5. Incubate new cartridges in the QX Cartridge Stand for at least 20 minutes prior to use. Note: Once used, the QIAxcel DNA gel cartridge must be stored in a vertical orientation. For more information, see “Storage”, page 6.

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Preparing the QIAxcel gel cartridge.

Preparing the buffer tray 1. Allow all reagents to equilibrate to room temperature (15–25°C) before use. 2. Wash the buffer tray with hot water and rinse thoroughly with deionized water. 3. Fill the WP and WI positions of the buffer tray with 8 ml QX Wash Buffer. 4. Fill the BUFFER position of the buffer tray with 18 ml QX Separation Buffer or, if using a QIAxcel DNA Fast Analysis Cartridge, 18 ml QX FA Separation Buffer. 5. Carefully add mineral oil to cover all 3 positions to prevent evaporation: add 2 ml mineral oil to positions WP and WI and add 4 ml mineral oil to position BUFFER. 6. Insert the buffer tray into the buffer tray holder so that the slots for the 12-tube strips face the front of the instrument. Preparing QX Alignment Marker 1. Load 15 µl QX Alignment Marker into each tube of a QX 0.2 ml 12-Tube Strip. 2. Add 1 drop of mineral oil to each tube, and place the strip into the MARKER1 position of the buffer tray.

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QIAxcel DNA Handbook 04/2013

Preparing the buffer tray and inserting the buffer tray into the buffer tray holder.

Installing a QIAxcel DNA gel cartridge and smart key 1. Remove the QIAxcel DNA gel cartridge from the QX Cartridge Stand. 2. Open the cartridge door and place the QIAxcel DNA gel cartridge into the QIAxcel or QIAxcel Advanced instrument. The cartridge description label should face the front and the purge port should face the back of the instrument. 3. Insert the smart key into the smart key socket. The smart key can be inserted in either direction. 4. Close the cartridge door. 5. The cartridge identifier, number of runs remaining, and cartridge type will be displayed automatically in the software when the cartridge smart key is inserted. Note: The system will not recognize the cartridge and will not operate if the smart key is not inserted. A

B

Installing the QIAxcel gel cartridge and smart key in the A QIAxcel and B QIAxcel Advanced instruments.

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Intensity calibration Every QIAxcel DNA gel cartridge requires intensity calibration prior to sample analysis. The intensities of each capillary are normalized and a factor is applied for every subsequent run. This corrects for natural intensity reading variations between each capillary in the cartridge. Intensity calibration using QIAxcel ScreenGel Software The data for each cartridge intensity calibration are stored in a file named according to the cartridge and instrument identifiers (<cartridgeid>_<instrument-id>.xcc). This file is saved either in the default directory C:Documents and SettingsAll UsersApplication DataQIAGENQIAxcelScreenGelDataCartridgeCalibrationData or in a customized directory. If, for any reason, a different computer is used to the one on which the calibration file is saved, the calibration file should be transferred to the new computer. Otherwise, recalibration of the cartridge is required. Similarly, if the QIAxcel gel cartridge is used on a different QIAxcel instrument to the one it was calibrated on, another intensity calibration should be performed. Intensity calibration of the cartridge takes about 16 minutes. 1. Load 15 µl QX Intensity Calibration Marker into each tube of a QX Colored 0.2 ml 12-Tube Strip. Add a drop of mineral oil, and insert the strip into the MARKER2 position of the buffer tray. 2. Launch the calibration run by clicking the “Start calibration” button in the “Calibration” screen of the “Service” environment. 3. Once the calibration is complete, the calibration results are displayed next to the gel image or the electropherogram view. The result table shows the area, calibration factor, and the result (“Pass” or “Fail”) for each channel. Note: A successfully calibrated cartridge should have a normalized area calibrated range between 0.004–0.006 or, if using a QIAxcel DNA Fast Analysis Cartridge, between 0.0035–0.0065. 4. If one or more channels show no signals in the first run, refer to Appendix D, page 44. 5. If one or more channels show high background, refer to Section 8 of the QIAxcel User Manual or the QIAxcel Advanced User Manual. 6. If calibration fails more than twice, call QIAGEN Technical Services. Recalibration using QIAxcel ScreenGel Software To recalibrate a cartridge, repeat the procedure described in “Intensity calibration using QIAxcel ScreenGel Software”. The calibration results of the previous calibration procedure are discarded when recalibrating a cartridge.

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QIAxcel DNA Handbook 04/2013

Note: It is possible to calibrate a cartridge for which no calibration runs remain. In this case, 3 of the remaining regular runs are used instead of 1 calibration run. Intensity calibration using BioCalculator Software The data (individual calibration data files) for each cartridge intensity calibration are stored in the CALdata folder. This folder is saved in the BioCalculator root directory: C:Program FilesQIAxcel BioCalculator. A Calibration2.log file (cartridge calibration information) is saved automatically in the BioCalculator root directory: C:Program FilesQIAxcel BioCalculator. If, for any reason, a different computer is used to the one on which the Calibration2.log file is saved, the Calibration2.log file should be transferred to the new computer. Otherwise, recalibration of the cartridge is required. Similarly, if the QIAxcel gel cartridge is used on a different QIAxcel instrument to the one it was calibrated on, another intensity calibration should be performed. Intensity calibration of the cartridge takes about 16 minutes. 1. Load 15 µl QX Intensity Calibration Marker into each tube of a QX Colored 0.2 ml 12-Tube Strip, make sure no air bubbles are trapped in the solution, and place it into the MARKER2 position of the buffer tray. 2. Launch the calibration wizard by selecting “File” then “Intensity Calibration” in the “Instrument Control” window. 3. Click “Start” to begin the cartridge intensity calibration. 4. When calibration is complete, the “Calibration Verification” dialog box will open. This will show either “Pass” or “Fail” for each channel. Note: A successfully calibrated cartridge should have a normalized area calibrated range between 0.004–0.006 or, if using a QIAxcel DNA Fast Analysis Cartridge, between 0.0035–0.0065. 5. If one or more channels fail, repeat the calibration process using a new strip of QX Intensity Calibration Marker (see page 18). 6. If one or more channels show no signals in the first run, refer to Appendix C, page 38. 7. If one or more channels show high background, refer to Section 8 of the QIAxcel User Manual or the QIAxcel Advanced User Manual. 8. If calibration fails more than twice, call QIAGEN Technical Services.

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Recalibration using BioCalculator Software Note: Use a new strip of QX Intensity Calibration Marker for each recalibration run. Re-using the intensity calibration marker may affect the calibration data and can cause them to be out of range. 1. Load 15 µl QX Intensity Calibration Marker into each tube of a new QX Color 0.2 ml 12-Tube Strip. Make sure there are no air bubbles, and place the strip into the MARKER2 position of the buffer tray. 2. Launch the calibration wizard by selecting “File” then “Intensity Calibration” in the “Instrument Control” window. 3. Click “Recalibrate” and then “Start” to repeat the calibration routine.

Sample preparation recommendations The minimum sample volume required for analysis is 10 µl. Less than 0.1 µl of the sample will be injected into the QIAxcel gel cartridge for analysis. The remaining DNA can be kept for reanalysis or use in downstream applications such as sequencing or cloning. We do not recommend using the remaining DNA in amplification-based applications such as real-time PCR or nested PCR.

Marker selection Alignment marker The QIAxcel DNA Fast Analysis Kit contains QX Alignment Marker 15 bp/3 kb (cat. no. 929522) which should be used in combination with the corresponding methods. For optimal DNA fragment size determination using the QIAxcel DNA High Resolution Kit or the QIAxcel DNA Screening Kit, select a QX Size Marker with a fragment size close to the size of your samples (see ordering information, page 51). Ensure that the fragment of interest is smaller than the largest peak of the chosen size marker. A product finder is available at www.qiagen.com/QXmarker to help with selection. For example, if analyzing a sample containing DNA fragments that are 100–400 bp in size, QX Alignment Marker 15 bp/600 bp (cat. no. 929530) should be used. For general applications using the QIAxcel DNA High Resolution Kit or the QIAxcel DNA Screening Kit, QX Alignment Marker 15 bp/5 kb (cat. no. 929524) is suitable. Alignment markers are injected from the MARKER1 position of the buffer tray and co-migrate with the DNA samples for analysis.

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QIAxcel DNA Handbook 04/2013

DNA size marker QIAxcel ScreenGel software or BioCalculator software calculates the DNA fragment size based on the fragment migration time in comparison to a reference QX DNA Size Marker (see ordering information, page 51). The DNA size (bp) is calculated using a point-to-point calculation using 2 DNA size marker fragments. To enable accurate size measurements, the size of the DNA fragments to be analyzed must fall within the smallest and largest fragment sizes of the QX DNA Size Marker. The QIAxcel DNA Fast Analysis Kit contains the ready-to-use QX DNA Size Marker 50 bp – 1.5 kb. This marker should be used undiluted and is suitable for most applications of the QIAxcel DNA Fast Analysis Kit. For optimal DNA size determination using the QIAxcel DNA High Resolution Kit or the QIAxcel DNA Screening Kit, select a QX DNA Size Marker with fragments closest to the size of your DNA sample. For optimal concentration determination, we recommend diluting the QX DNA Size Marker in the same buffer that was used for the DNA samples. For undiluted DNA samples, we recommend diluting the 100 ng/µl QX DNA Size Marker in 1x PCR buffer or 1x restriction digestion buffer to the following final concentration:



10 ng/µl for L methods



30 ng/µl for M methods



50 ng/µl for H methods

For samples diluted in QX DNA Dilution Buffer, we recommend also diluting the DNA size marker to a final concentration of 5 ng/µl in QX DNA Dilution Buffer. Table 2 provides recommendations for combining QX DNA Size Markers and QX Alignment Markers.

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Table 2. Recommended QX DNA Size Marker and QX Alignment Marker combinations Size marker

Cat. no. Alignment marker

Cat. no.

QX DNA Size Marker pUC18/HaeIII (50 µl)

929550 QX Alignment Marker 15 bp/1 kb (1.5 ml)

929521

QX DNA Size Marker FX174/HaeIII (50 µl)

929551 QX Alignment Marker 15 bp/3 kb (1.5 ml)

929522

QX DNA Size Marker 25 bp – 500 bp (50 µl)

929560 QX Alignment Marker 15 bp/600 bp (1.5 ml)

929530

QX DNA Size Marker 100 bp – 929559 QX Alignment Marker 2.5 kb (50 µl) 15 bp/3 kb (1.5 ml)

929522

QX DNA Size Marker 50–800 bp (50 µl) v2.0

929561 QX Alignment Marker 15 bp/1 kb (1.5 ml)

929521

QX DNA Size Marker 250 bp – 929562 QX Alignment Marker 4 kb (50 µl) v2.0 50 bp/5 kb (1.5 ml)

929529

QX DNA Size Marker 250 bp – 929563 QX Alignment Marker 8 kb (50 µl) v2.0 15 bp/10 kb (1.5 ml)

929523

Method selection A number of preinstalled methods are available for QIAxcel DNA gel cartridges. To select the appropriate method for the samples being analyzed, refer to Appendix C, page 38, or Section 5.5 of the QIAxcel User Manual or Section 6.3.3 of the QIAxcel Advanced User Manual.

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