Glucose 201 with Plasma Conversion Operating Manual July 2014

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Table of Content HemoCue® Glucose 201 system ... 3 Components... ... 4 Start up ... 6 Audio signal ... 8 Determination ... 10 Capillary blood... 10 Venous and arterial blood.. ... 18 Maintenance... ... 20 Daily maintenance... 20 Optical parts ... 22 Troubleshooting Guide ... 24 Specifications ... ... 27

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HemoCue Glucose 201 with Plasma Conversion Operating Manual

The HemoCue® Glucose 201 Analyzer with plasma conversion provides laboratory quality analysis of glucose in whole blood, easily, quickly and conveniently. Utilization of a conversion factor allows the measured whole blood result to be displayed as a plasma equivalent glucose value. Capillary, venous or arterial whole blood may be used. This manual provides the basic instructions for routine use as well as technical specifications. Further information may be obtained from HemoCue America or your local distributor. All system components are designed and manufactured to provide maximum safety. Any other use of the system may impair the safety.

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Components

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QRG 1

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5

4

6

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1.

The HemoCue Glucose 201 Analyzer*

2.

AC adapter.**

3.

4 type AA or R6 batteries, 1.5 V.***

4.

A vial of HemoCue Glucose 201 Microcuvettes. Individually packaged HemoCue Glucose 201 Microcuvettes.***

5.

HemoCue Glucose 201 Operating manual and Quick Reference Guide.

6.

HemoCue Cleaner.

The HemoCue Glucose 201 Analyzer and power adapter are delivered in a carton. Open the carton on a stable surface and lift out the analyzer and the accessories. Consult local environmental authorities for proper disposal of batteries. * Do not open the cover of the analyzer. Note: The warranty is voided if the analyzer has been opened ** Only use adapters as listed under Specifications. *** Not included.

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Start-up

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1a

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1b

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1a. If A/C power is available, plug the power adapter into the power inlet at the back of the analyzer. 1b. If A/C power is not available, install 4 type AA batteries in the battery compartment as shown. 2.

Pull the cuvette holder out to the loading position.

3.

Press and hold the on/off button until the display is activated.

4.

The display shows ”SELFTEST” and the version number of the program, after which it will say ”GLU”. During the ”SELFTEST” the analyzer will automatically verify the performance of the optronic unit.

5.

After 15 seconds the display will show ”READY” and three flashing dashes. This indicates that the HemoCue Glucose 201 Analyzer is ready for use.

To turn the analyzer off, press and hold the on/off button until the display is turned off.

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Audio signal

2a

2b

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To activate an audio signal which will indicate when the result is displayed, follow the instructions below. 1.

Check that the analyzer is turned off. The display should be blank. Pull the cuvette holder out to the loading position.

2a,b. Press and hold the on/off button until the display shows a bell in the upper right corner. The audio signal is now activated. 3.

Wait until the analyzer display says ”READY” before performing an analysis.

To turn off the audio signal follow steps 1– 3 until the bell in the upper right corner disappears. The analyzer is delivered with the audio signal turned off.

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Measuring Capillary blood

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Note: In cases of severe hypotension or peripheral circulatory failure, glucose measurement from capillary samples may be misleading. In such circumstances it is recommended that venous or arterial whole blood be used.1 1.

After start-up, the cuvette holder should be in the loading position. The display will show “READY” and three flashing dashes.

2.

Make sure the patient’s hand is warm and relaxed. Use only the middle or ring finger for sampling. Avoid fingers with rings on.

3.

Clean with disinfectant and allow to dry or wipe off with a dry, lint free tissue.

4.

Using your thumb, lightly press the finger from the top knuckle towards the tip. This stimulates the blood flow towards the sampling point.

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5.

For best blood flow and least pain, sample at the side of the fingertip, not in the center.

6.

While applying light pressure towards the fingertip, puncture the finger using the lancet.

7.

Wipe away the first 2 or 3 drops of blood.

8.

Re-apply light pressure towards the fingertip until another drop of blood appears.

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9.

When the blood drop is large enough, fill the microcuvette in one continuous process. The microcuvette shall be completely filled. Do NOT

refill! 10. Wipe off excess blood from the outer surface of the microcuvette with lint free tissue, being careful not to touch the open end of the microcuvette. 11. Look for air bubbles in the filled microcuvette. If present, take a new sample. Small bubbles around the edge can be ignored. Note: If a second sample is to be taken from the same fingerstick, it is important that this should be done after the first sample has been read. Wipe away the remains of the first sample and take a second one from a new drop of blood.

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12. Place the microcuvette into the cuvette holder and start measurement as soon as possible but no later than 40 seconds after filling the microcuvette by gently pushing the cuvette holder to its measuring position.

13. During the measurement, “6” will be shown in the display.

14. After 40 –240 seconds, the glucose value of the sample is displayed. The result will remain on the display as long as the cuvette holder is in the mesuring position. When operating on battery power, the analyzer will automatically turn off after approximately 10 minutes. The result can be retrieved by pressing the on/off button until the display is reactivated. DO NOT remeasure the microcuvette! 15. Although the reagents are present in the microcuvette in extremely low quantities, consult local environmental authorities for proper disposal. Always handle blood specimens with care, as they might be infectious.

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Measuring Venous and arterial blood

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2a

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2b

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1.

The blood should be well mixed prior to performing the measurement.

2a,b. Place a drop of blood onto a hydrophobic surface, e.g., a plastic film, using a pipette, a DIFF-Safe™or directly from a syringe. 3.

Fill the microcuvette in one continuous process. The microcuvette shall be completely filled. Do NOT refill! Wipe off excess blood from the outer surface of the microcuvette with lint free tissue, being careful not to touch the open end of the microcuvette.

4.

Look for air bubbles in the filled microcuvette. If present, take a new sample. Small bubbles around the edge can be ignored.

Perform the analysis as per steps 12–15 on page 17.

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Maintenance Daily maintenance 2 1

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The cuvette holder should be cleaned after each day of use. 1. Pull the cuvette holder out to the loading position. 2. While pressing the catch, carefully rotate the cuvette holder sideways in open position as far as possible to the left. 3. Remove the cuvette holder from the analyzer, it will come off the stainless steel pin it rotates on. 4. Clean the cuvette holder with alcohol (20–70 %) or mild detergent. Wait 15 minutes before replacing the cuvette holder and using the analyzer. Make sure the cuvette holder is dry before inserting. The optical parts should be cleaned when directed to do so in the Troubleshooting Guide, see Maintenance Optical parts.

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Maintenance Optical parts 5a

5b

6

5c

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Dirty optical parts may cause an error code. Follow step 1–3 under Daily maintenance and then clean optical parts as follows. Note! Make sure that the HemoCue® Cleaner reaches both upper and lower cover glasses, see pictures 5a and 5c. 5. With the cuvette holder removed from the analyzer push the Cleaner into the opening of the optic unit, as far in as possible. Move from side to side 5–10 times, see picture 5b and thereafter push in and pull out the Cleaner 5–10 times, cleaning the cover glasses, placed to the left, see picture 5c. If the Cleaner is stained, repeat with a new Cleaner. 6. Wait 15 minutes before replacing the cuvette holder and using the analyzer. Make sure the cuvette holder is dry before inserting. The cover may be cleaned with alcohol (20–70 %) or mild detergent. Note! As an alternative to the HemoCue® Cleaner, a lint free cotton swab, non-pretreated, moistened with alcohol (20–70 % without additive) or water may also be used. If a cotton swab is used make sure it is not too wet and not too dry. Use a dry swab to wipe away excess liquid in the optic house after cleaning with a moistened swab. To avoid scratches on the cover glasses, only the cotton part of the swab should come in contact with the cover glasses.

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