Saturn 5 Laser Systems User Manual Issue 11

CONTENTS SECTION 1 - PREFACE

4

SECTION 2 - INTRODUCTION TO THE SATURN 5 LASER SYSTEM

5

Intended Use Indication for Use - USA Customers Only Applicable Part Numbers Microscope Compatibility Installation

5 5 5 5 6

SECTION 3 - SAFETY WARNINGS

7

Precautions Guidance and Manufacturer’s Declaration – Electromagnetic Emissions (IEC 60601-1-2) Guidance and Manufacturer’s Declaration - Electromagnetic Immunity

9 9 10

SECTION 4 - PRODUCT OVERVIEW

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System Components Saturn 5 Laser System Component Part Numbers Saturn 5 Active Laser System Mirror Module Part Numbers Saturn 5 Laser System Collimator Module Part Numbers Hardware Overview Laser Specifications Objective Specifications  Electrical Specifications  Mains Adaptor for Control Unit and Motor Module Dimensions  Operating Range 

13 14 14 15 15 16 16 16 16 16 16

SECTION 5 - RI VIEWER 

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Introduction User Interface Icons How to Zoom & Pan the Image How to Set the Preset Zoom How to Select Cameras How to View Live Images Camera Flipping Changing Camera Settings Objectives How to Select Objectives How to Add Objectives How to Configure the Foot Pedal/Keyboard How to Remove Objectives  How to Check Objective Calibration How to Perform Measurements How to Record Video RI Viewer Gallery  Advice for Use

17 17 18 18 18 18 19 19 19 19 19 20 20 20 21 21 22 27

SECTION 6 - SATURN 5 LASER SYSTEM INTENDED USES

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Laser Assisted Hatching Embryo Biopsy procedure Cleavage Stage Blastomere Biopsy Trophectoderm /Blastocyst Biopsy Blastocyst Collapse

28 28 28 29 29

SECTION 7 - LASER ABLATION PROCEDURE

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Hole Size Selection Ablation Procedure 

31 31

SECTION 8 - LASER OPERATION

33

How to Startup How to Configure the Laser for a New Procedure How to Check Laser Target Alignment for Saturn 5 Active Laser System How to Check Laser Target Alignment for Saturn 5 Fixed Laser System How to Check Hole Size Calibration How to Remove a Point from the Hole Size Calibration Graph Laser Target Hole Size Indicator  Exclusion Zone How to Adjust Hole Size/Pulse Width Preset Pulse Lengths How to Fire the Laser (Single Pulse) Biopsy Mode USA Clearance Regulations How to Enable Biopsy Mode How to Fire the Laser (Biopsy Mode) How to Shut Down

33 33 33 33 34 34 35 35 35 35 35 36 36 36 36 37 37

SECTION 9 - TROUBLESHOOTING FOR SATURN 5 LASER SYSTEM

38

SECTION 10 - CARE AND MAINTENANCE

39

Cleaning Servicing Disposal of Goods

39 39 39

SECTION 11 - REPAIRS AND RETURNS PROCEDURES

40

RI Repairs System  40 RI Returns System 40 Contact Details 40 Obligation to Inform 40 Feedback40

Section 1 Preface

1

Research Instruments Ltd

SECTION 1 - PREFACE Thank you for choosing the Saturn 5 Laser System. This manual provides all necessary information to use the Research Instruments Ltd Saturn 5 Laser System. The system should be operated by trained personnel only. All sections of this manual should be read and understood fully before any operation of the system. Please see the Intended Use for more information. If the operator is unsure of any of the information contained in this manual, they should contact Research Instruments or an appointed representative before attempting to use this equipment. In no event does Research Instruments Ltd (RI) assume the liability for any technical or editorial errors of commission, or omission; nor is Research Instruments Ltd liable for direct, indirect, incidental, or consequential damages arising out of the use or inability to use this manual. © This manual is protected by copyright, all rights reserved, and no part hereof may be photocopied or reproduced in any form without the prior written consent of Research Instruments Ltd. Saturn™ and RI Viewer™ are trademarks of Research instruments Ltd. All other brand names are trademarks of their respective owners. The information in this manual is current at the time of publication. RI is constantly updating its products, and therefore reserves the right to introduce changes in design, equipment and technical features at any time. The latest version of the User Manual as well as RI Viewer software can be downloaded from software.research-instruments.com. The Saturn 5 Laser System manual belongs with the laser and should be passed on with the Saturn 5 Laser System if relocated to another clinic.

This indicates cautionary text which should be followed to avoid injury to users or damage to samples. The system should be operated by qualified and trained personnel only.

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Research Instruments Ltd

Section 2

Introduction to Saturn 5™ Laser Systems

SECTION 2 - INTRODUCTION TO THE SATURN 5 LASER SYSTEM Intended Use

2

The Saturn Laser System is intended ‘to ablate a User defined section of a gamete or embryo within ART’.

0120

Indication for Use - USA Customers Only For use in assisted reproduction procedures to ablate or thin the zona pellucida of an embryo to facilitate assisted hatching or recovery of cells for pre-implantation genetic diagnosis (blastomeres). The device can also be used on blastocyst stage embryos for biopsy of trophectoderm cells for pre-implantation diagnosis procedures, and blastocyst collapse prior to vitrification procedures.

Only Caution: Federal law restricts this device to sale by or on the order of a physician or a practitioner trained and certified in its use Applicable indications for use are subject to the regulations of the country into which the device is sold.

Applicable Part Numbers 6-47-500 - Saturn 5 Active Laser System 6-47-501 - Saturn 5 Fixed Laser System

Microscope Compatibility Zeiss Axiovert 40/100/200/Observer Nikon TMD, D200/300, TE200/300, TE2000, Ti Leica DMIRB, DMI3000B/4000B/6000B, DMIL, DMi8 Olympus IMT2, IX50/70, IX51/71/81, IX53/73/83

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Section 2

Introduction to Saturn 5™ Laser Systems

Research Instruments Ltd

Installation The PC is an intrinsic part of the Saturn 5 Laser System. It may be connected to a Local Area Network (LAN) only. Only install RI Viewer software. We recommend PC security features such as anti-virus software/firewall be installed consistent with the facility’s IT policy and that each user of the system has a password protected user profile assigned to the PC in order to prevent unauthorised access.

2

Installation of the Saturn 5 Laser System should be carried out by a Research Instruments technician or other RI-authorised personnel. Incorrect installation could result in reduced power output, laser misalignment or laser malfunction. Relocation of this system should be treated as a re-installation and should, therefore, be carried out by authorised personnel. Please note that installation and servicing is covered separately in the Saturn 5 Laser System Installation Manual (6-47-500IM). Users within the USA will not be provided with the Saturn 5 Laser System Installation Manual. A Patients Guide to Laser Assisted Hatching (LAH), Laser Assisted Biopsy (LAB) and Blastocyst Collapse are provided on the RI Viewer installation CD. These can be opened using Adobe Reader or other PDF viewers. We recommend that copies are printed and made available to patients.

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Research Instruments Ltd

Section 3

Saturn™ 5 Laser Systems Safety Warnings

SECTION 3 - SAFETY WARNINGS Laser radiation is present within the control unit, the fibre optic patch lead and through the microscope to output at the objective. The Saturn 5 Laser System has been designed to ensure that emission of laser radiation at any point is within the limits stipulated by international safety standards, IEC 60825-1:2014, and US 21CFR1040.10 for unprotected viewing (Class 1). As an extra precaution, an infrared blocking filter has been incorporated into the optical path leading to the eyepieces to reduce laser emission by a further 99%.

3

For a description of controls and for the location of laser aperture on control unit, see Section 4 Hardware Overview. CAUTION Hazardous radiation exposure may occur through the use of controls, executing adjustments or the performance of procedures other than those specified herein. Servicing and adjustment other than that specified in this manual should only be carried out by RI or RI authorised agents. DO NOT disassemble or modify any part of the Saturn 5 Laser System, or substitute any component for any other. Doing so may result in exposure to dangerous levels of laser radiation. This voids the warranty and/or service contract.

DO NOT use a magnifying glass or eye loupe above the objective lens.

DO NOT operate the laser unless it is properly mounted to a microscope.

ONLY use the power cable and power supply adaptor supplied with the system. The cable to the power supply is the Disconnect Device for this equipment. To remove all electrical power from this product, disconnect the power cable from the electrical outlet. Equipment should be positioned so as to allow easy access to the power cable. The appliance coupler or mains plug is used as the disconnect and must remain readily operable. WARNING To avoid the risk of electric shock, this equipment must only be connected to a supply mains with protective earth.

WARNING Not to be used in a patient environment

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Section 3

Saturn™ 5 Laser Systems Safety Warnings

Research Instruments Ltd

WARNING Before performing any procedure, the pilot laser must be used to check the alignment of the Active positioning system. The system must be re-aligned when necessary. See Section 8 How to Check Laser Target Alignment. WARNING Thermal lensing is a defocusing of the laser beam caused by changes to the refractive index of the medium as it is heated. The degree of defocus depends on the total beam energy deposited in the medium. While slight defocus has no effect on the drilling properties, this effect may become a problem for higher beam energies. To eliminate any significant effects from thermal lensing, we recommend that the pulse length be kept below 1000μs for clinical Laser Assisted Hatching (LAH) zona drilling.

3

WARNING Only a single opening should be made in the zona pellucida. Multiple openings or those that are too small may prevent embryo hatching or lead to abnormal development.

WARNING Do not operate the pilot laser with an embryo in the field of view. Exposure to the pilot laser may damage the embryo. WARNING The microscope itself must be maintained to a high standard. Problems such as worn focus mechanisms or an insecure video camera may lead to unreliable focus and image stability, and could lead to embryo damage. WARNING Do not operate the laser without the RI Viewer software running, as targeting and hole size indication will not be active, and the embryo may be damaged. WARNING The embryo may be damaged if objectives other than the red Saturn 5 Laser System objective is used.

WARNING Do not modify this equipment without authorisation of the manufacturer.

WARNING Where an ITO glass heated stage is fitted, do not fire the laser with the microscope focussed into the ITO glass surface as this may damage the ITO coating.

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Section 3

Research Instruments Ltd

Saturn™ 5 Laser Systems Safety Warnings

Precautions •

To minimise the risk of damage to blastomeres, administer as few laser pulses as possible at the lowest energy levels possible to achieve the prescribed zona drilling or thinning effect.

•

Direct the laser beam toward a section of the zona pellucida where the adjacent perivitelline space is widest or next to an area of fragmentation.

•

A holding pipette should be used during laser treatment to minimise the risk of embryo movement.

•

Small openings in the zona pellucida may lead to embryo constriction and abnormal development.

•

To date there are no known reports showing a greater occurrence rate of major or minor defects in children derived from laser-hatched embryos. Long-term follow-up data on children born from laser-hatched embryos does not yet exist. A study of 134 such babies* found no increase in the major congenital malformations, chromosomal aberrations or minor congenital malformations between the LAH treated group and all deliveries at their hospital.

•

The device is not affected by and does not present any reciprocal interference to the microscope.

•

During normal use there is no contact with patient/sample.

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*Kanyo, K., Konc, J. “A follow-up study of children born after diode laser assisted hatching.” European Journal of Obstetrics and Gynaecology. 110: 176-180 (2003).

Guidance and Manufacturer’s Declaration – Electromagnetic Emissions (IEC 60601-1-2) The Saturn 5 Laser System is intended for use in the electromagnetic environment specified below. The customer or the user of the Saturn 5 Laser System should assure that it is used in such an environment. Emissions test

RF emissions CISPR 11

RF emissions CISPR 11 Harmonic emissions IEC 61000-3-2 Voltage fluctuations/flicker emissions

Compliance

Group 1

Class B

Class A Complies

Electromagnetic environment guidance The Saturn 5 Laser System uses RF energy only for its internal function. Therefore, its RF emissions are very low and are not likely to cause any interference in nearby electronic equipment. The Saturn 5 Laser System is suitable for use in all establishments, including domestic establishments and those directly connected to the public low-voltage power supply network that supplies buildings used for domestic purposes.

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Section 3

Saturn™ 5 Laser Systems Safety Warnings

Research Instruments Ltd

USA Only Compliance with the emissions requirements of CISPR 22 Class A requires the following warning: “This is a class A product. In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures.”

Guidance and Manufacturer’s Declaration - Electromagnetic Immunity

3

The Saturn 5 Laser System is intended for use in the electromagnetic environment specified below. The customer or the user of the Saturn 5 Laser System should ensure that it is used in such an environment. IMMUNITY test

IEC 60601 Test level

Compliance level

Electro magnetic environment guidance

Electrostatic discharge (ESD) IEC 61000-4-2

± 6 kV contact ± 8 kV air

± 6 kV contact ± 8 kV air

Floors should be wood, concrete or ceramic tile. If floors are covered with synthetic material, the relative humidity should be at least 30 %.

Electrical fast transient/burst IEC 61000-4-4

± 2 kV for power supply lines ± 1 kV for input/ output lines

± 2 kV for power supply lines ± 1 kV for input/ output Lines

Mains power quality should be that of a typical commercial or hospital environment.

Surge IEC 61000-4-5

± 1 kV line(s) to ± 1 kV differential line(s) Mode ± 2 kV line(s) to earth ± 2 kV common mode

Mains power quality should be that of a typical commercial or hospital environment.

Voltage dips, short interruptions and voltage variations on power supply input lines IEC 61000-4-11

<5% UT (>95% dip in UT ) for 0.5 cycle 40% UT (60% dip in UT) for 5 cycles 70% UT (30% dip in UT) for 25 cycles <5 % UT (>95 % dip in UT) for 5s

<5 % UT (>95 % dip in UT) for 0.5 cycle 40 % UT (60 % dip in UT) for 5 cycles 70 % UT (30 % dip in UT) for 25 cycles <5 % UT (>95 % dip in UT) for 5s

Mains power quality should be that of a typical commercial or hospital environment. If the user of the Saturn 5 Laser System requires continued operation during power mains interruptions, it is recommended that the Saturn 5 Laser System be powered from an uninterruptible power supply or a battery.

Power frequency (50/60 Hz) magnetic field

3 A/m

0.3 A/m

The power frequency magnetic field should be measured in the intended installation location to assure that it is sufficiently low.

IEC 61000-4-8

Note: UT is the a.c. mains voltage prior to application of the test level.

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Section 3

Research Instruments Ltd

Saturn™ 5 Laser Systems Safety Warnings

Guidance and Manufacturer’s Declaration - Electromagnetic Immunity Continued IMMUNITY test

IEC 60601 Test level

Compliance Electro magnetic environment - guidance level

Conducted RF

3 Vrms

3 Vrms

IEC 61000-4-6

150 kHz to 80 MHz

Radiated RF

3 V/m

IEC 61000-4-3

80 MHz to 2.5 GHz

3 V/m

Portable and mobile RF communications equipment should be used no closer to any part of the Saturn 5 Laser System including cables, than the recommended separation distance calculated from the equation applicable to the frequency of the transmitter.

3

Recommended separation distance d = 1,2√P d = 1,2√P 80 MHz to 800 MHz d = 2,3√P 800 MHz to 2 , 5 GHz where P is the maximum output power rating of the transmitter in watts (W) according to the transmitter manufacturer and d is the recommended separation distance in metres (m). Field strengths from fixed RF transmitters, as determined by an electromagnetic site survey, a should be less than the compliance level in each frequency range. b Interference may occur in the vicinity of equipment marked with the following symbol:

Note 1: At 80 MHz and 800 MHz, the higher frequency range applies. Note 2: These guidelines may not apply in all situations. Electromagnetic propagation is affected by absorption and reflection from structures, objects and people. Field strengths from fixed transmitters, such as base stations for radio (cellular/cordless) telephone and land mobile radios, amateur radio, AM and FM radio broadcast and TV broadcast cannot be predicted theoretically with accuracy. To assess the electromagnetic environment due to fixed RF transmitters, an electromagnetic site survey should be considered. If the measured field strength in the location in which the Saturn 5 Laser System is used exceeds the applicable RF compliance level above, the Saturn 5 Laser System should be observed to verify normal operation. If abnormal performance is observed, additional measures may be necessary, such as re-orienting or relocating the Saturn 5 Laser System. b Over the frequency range 150 kHz to 80 MHz, field strengths should be less than 3 V/m. a

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Section 3

Saturn™ 5 Laser Systems Safety Warnings

Research Instruments Ltd

Safety/Information Symbols Symbol

Meaning Indicates instruction for disposal of goods.

3 In accordance with Annex II of the European Medical Device Directive 93/42/EEC, as amended by Directive 2007/47/EC under the supervision of notified body No.0120, SGS, UK Ltd.

0120

Indicates the medical device manufacturer. Indicates the need for the user to consult the instructions for use for important cautionary information such as warnings and precautions that cannot, for a variety of reasons, be presented on the medical device itself.

Follow instructions for use.

SN

Only

CLASS 1 LASER PRODUCT

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The first four digits are the serial number, a unique identifier assigned to the product. The last four digits signify the month and year of manufacture, eg 5001/0214 (this denotes a unique serial number of 5001 and confirms manufacture in February 2014). Caution: US Federal law restricts this device for sale to or on the order of a licensed healthcare practitioner. Class 1 laser product

Research Instruments Ltd

Section 4

Saturn™ 5 Laser Systems Product Overview

SECTION 4 - PRODUCT OVERVIEW Welcome to the User Manual for the Research Instruments Saturn 5 Laser System. This manual covers the set-up, operation and maintenance of the system. For use in assisted reproduction procedures to ablate or thin the zona pellucida of an embryo to facilitate assisted hatching or recovery of cells for pre-implantation genetic diagnosis (blastomeres). The device can also be used on blastocyst stage embryos for the biopsy of trophectoderm cells for pre-implantation diagnosis procedures, and blastocyst collapse prior to vitrification procedures. The system is used with an inverted microscope, PC and camera to provide an optical system and means of firing a laser. The system cannot be used without a PC or RI viewer software. The Saturn 5 Laser System is offered in two options, fixed or active models. The active model allows the user to move the position of the laser to the target area on the sample. The fixed model has the laser in a fixed position and the user moves the sample to the target area.

4

All laser movement (Active model only) and laser firing is user controlled.

System Components Power Supply Mirror Module

Control Unit Objective

Motor Module

Patch Lead Note Actual components may differ from those shown. The Saturn 5 Laser System comes supplied with the following parts, a control unit containing the lasers, mirror module, armoured patch lead and a special objective. If you have a Saturn 5 Active Laser System then an additional motor module will be supplied. The laser beams are delivered to the specimen through a fibre optic patch lead, collimator module (that is part of the mirror module for a fixed system or the motor module for an active system) and the special objective. A foot pedal for firing the laser is available as an optional extra. This is attached to the PC by a mini USB cable. The motor module receives coordinates via the USB which moves the motors to those coordinates. The control unit then receives commands from the software to fire for a specific length of time.

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Section 4

Saturn™ 5 Laser Systems Product Overview

Research Instruments Ltd

The following tables give part numbers of system components and microscope adaptors. All parts are supplied or manufactured by Research Instruments Ltd.

Saturn 5 Laser System Component Part Numbers Part Number 5-47-010 5-47-100 1-10-100 5-45-500 5-45-507

4

Description Control Unit with 1.5m Patch Lead Motor Module (Saturn 5 Active Laser System) 12V 2.5A Medical PSU (x2 for Saturn 5 Active Laser System) Laser Objective (red) 40x Laser Objective (red) 20x*

Saturn 5 Active Laser System Mirror Module Part Numbers Part Number 5-46-163 5-46-150 5-46-160 5-46-151 5-46-152 5-46-159 5-46-135 5-46-132 5-46-130 5-46-140 5-46-141 5-46-142 5-46-144 5-46-158 5-46-110 5-46-120 5-46-122 5-46-121 5-46-124

Description Mirror module for Olympus IX53/73/83 left Mirror module for Olympus IX50/70 left Mirror module for Olympus IX50/70 right Mirror module for Olympus IX51/71/81 left Mirror module for Olympus IX51/71/81 right Mirror module for IMT2 Mirror module for TMD Mirror module for Nikon Diaphot 200/300 Mirror module for TE 200/300 Mirror module for TE2000 with Integra Ti Mirror module for TE2000 without Integra Ti Mirror module for Nikon Ti with Integra Ti Mirror module for Nikon Ti with X-Y Mirror module for Axiovert 40 Mirror module for Axiovert 200/Observer Mirror module for DMIRB Mirror module for DMI3000B Mirror module for DMI4000B/6000B Mirror module for DMi8

*Not cleared for use in USA market.

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Section 4

Research Instruments Ltd

Saturn™ 5 Laser Systems Product Overview

Saturn 5 Laser System Collimator Module Part Numbers 5-45-163 5-45-383 5-45-387 5-45-388 5-45-580 5-45-411 5-45-301 5-45-545 5-45-546 0120 5-45-575 only 5-45-009 5-45-011 5-45-450 5-45-470 5-45-461 5-45-460 5-45-463

Collimator module for Olympus IX53/73/83 Collimator module for Olympus IX50/70 Collimator module for Olympus IX51/71/81 Collimator module for Olympus IX71/71/81 fluor Collimator module for IMT2 Collimator module for TMD Collimator module for Nikon Diaphot 300/TE300 Collimator module for TE2000/Nikon Ti FOLLOW 1 CollimatorINSTRUCTIONS module for TE2000/NikonCLASS Ti fluor FOR USE 12V 2.5A LASER PRODUCT Collimator module for Axiovert 40 Saturn Laser System QC Research Instruments Ltd, LASER OUTPUT Bickland Industrial Park, module for Axiovert 100 PILOT Collimator Falmouth, Cornwall, UK KEEP CLEAN TR11 4TA USE AC ADAPTOR 1-10-100 PC Collimator module for Axiovert 200/Observer MANUFACTURED IN UNITED KINGDOM www.research-instruments.com Collimator module for DMIRB Collimator module for DMIL Collimator module for DMI3000B Collimator module for DMI4000B/6000B Collimator module for DMi8

4

Hardware Overview Rear Panel FOLLOW INSTRUCTIONS FOR USE

0120 only

PILOT

CLASS 1 LASER PRODUCT

Saturn Laser System Research Instruments Ltd, QC Bickland Industrial Park, Falmouth, Cornwall, UK TR11 4TA www.research-instruments.com MANUFACTURED IN UNITED KINGDOM

PC

12V

2.5A

LASER OUTPUT KEEP CLEAN

USE AC ADAPTOR 1-10-100

Pilot laser brightness: The brightness of the pilot laser can be adjusted by turning the control knob on the rear panel to obtain the clearest image in the target alignment procedure. Graphic PART NUMBER: 2-47-012AR Note: part number changed from SATURN REAR PANEL ARTWORK 2-47-012 to 2-47-012AR follow The light on the front of the control unit indicates that the controltounit is operational. If the unit is ECN: 1164 RI numbering convention. Issue plugged Instruments for assistance. ISSUE 4 in and the light is not on, contact Research numbers continue previous sequence. DRAWN: DP DATE:20/12/13

Pantone Reference Pantone: 300 Pantone: Black Pantone: White

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Section 4

Saturn™ 5 Laser Systems Product Overview

Research Instruments Ltd

Laser Specifications Laser Specifications Output wavelength Power output from patch lead Maximum pulse length *Laser Safety Classification

Ablation Laser 1480nm 400mW 2.0ms Class 1

Pilot Laser 650nm 180µW (max) None Class 1

* IEC 60825-1:2014, US 21CFR 1040.10

Objective Specifications

4

Magnification N.A. Working distance Parfocal distance

40x 20x* 0.49 0.25 2.5mm 2.5mm 45mm (adaptor supplied for Nikon CFI60 systems)

*Not cleared for use in USA market. Electrical Specifications Mains Adaptor for Control Unit and Motor Module Input Output Power (maximum) Voltage Current (maximum) Earth Connection

100-240VAC, 50-60Hz, 0.8-0.4A 30W 12VDC 2.50A Mandatory 3-pin plug for earthing (grounding)

Dimensions Control unit Weight

220mmx180mmx34mm (8.6”x7.1”x1.3”) 1.2Kg

Operating Range Temperature Humidity

16

10°C (50°F) to 42°C (108°F) 15% to 85% RH (Non Condensing)

Section 5 RI Viewer™

Research Instruments Ltd

SECTION 5 - RI VIEWER Introduction RI Viewer is the software that interacts with the Saturn 5 Laser System for use within an ART laboratory. Connected to a camera attached to a microscope, it provides on screen images of samples under the microscope. It can record video and store still images from the microscope in a PC’s file system. Supplied PCs are IEC 60950-1 approved. The operator should be positioned in such a place to easily access the microscope and also view the image on the PC monitor. RI Viewer software is provided in a CD format but can be downloaded from the RI website. Once installed the RI Viewer application can be started by double clicking the RI Viewer icon on the desktop.

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User Interface Icons Icon

Meaning

Icon

Meaning

Take Picture

Laser Control

Take Video

General Settings

Stop Video Recording

Additional Camera Settings

Open RI Viewer Gallery

Take Measurements

Choose Objective Magnification

Display Rulers Tool

Increase/Decrease Zoom Level

Instructions For Use

17

Section 5 RI Viewer ™

Research Instruments Ltd

How to View Live Images A camera will need to be connected to the PC running RI Viewer and the drivers installed correctly to view live images within RI Viewer. The dongle must also be fitted to a USB port. RI Viewer software will automatically recognise both analogue and compatible digital camera devices connected to the PC. If there is only one camera device detected, it will show the live image for that device. If multiple camera devices are connected, it will use the camera that was last selected from the Video Source drop down box. If there are multiple cameras attached to the PC, you may select the device you want the live image to be viewed from. To do this select the camera from the dropdown list on the Settings panel.

How to Zoom & Pan the Image There are three ways to zoom into an area on the screen.

5

1. Place the mouse at a point on the screen and click and hold the right mouse button then release. This will zoom into the spot where the mouse is placed. To zoom out, click and hold the right mouse button. 2. Place the mouse at a point on the screen and use the scroll wheel on the mouse (if available).This will give the user control over how much digital zoom is given. 3. Using the tool bar at the bottom of the screen, click the + icon to zoom in incrementally. To zoom out by the same amount, click on the - icon on the tool bar. The magnifying glass icon will zoom in and out by the preset zoom. Whilst using digital zoom, a thumbnail image of the screen will appear in the top left of the screen. At the top of this panel is the amount of magnification you are using. When zoomed out fully, this panel will not appear on the screen. Zooming in and zooming out can also be achieved using the foot pedal.

How to Set the Preset Zoom 1. Click the Additional Camera Settings Icon on the tool bar. 2. Drag the Preset Zoom slider to the required zoom.

How to Select Cameras 1. Click the General Settings Icon on the tool bar. The Video Source drop down box will have a list of camera names referring to the cameras connected to the PC. 2. Click on the drop down box to allow the selection of cameras. Clicking on the required camera in the list will change the live image to that of the selected camera. Note: The list of available cameras is automatically populated to show the attached devices. Camera names in this list cannot be altered by the user.

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Research Instruments Ltd

Section 5 RI Viewer™

Camera Flipping 1. Click the General Settings Icon on the tool bar. 2. Tick Flip Video Horizontal (for camera devices that support these options). 3. Tick Flip Video Vertical (for camera devices that support these options). Camera flipping is for those microscope set ups where the camera may be required to be inverted. This is carried out during installation where a camera port selected for one requires camera flipping to show the image correctly on the screen. This will be carried out by a trained RI Service Engineer or company representative. When camera flipping is required it is recommended to carry out Laser Target Alignment as per instructions before carrying out any procedure.

Changing Camera Settings Clicking the Additional Camera Settings icon on the tool bar will show a panel indicating the camera name and frames per second (fps). Clicking on Additional Settings will allow the user to adjust settings on the video source. Only settings available to the video source chosen will appear.

5

Objectives Objectives can be changed on the microscope to enable the user to get a better field of view or better magnification. If you require accurate measurements of the image, the objective chosen in RI Viewer must be the same magnification as the objective chosen in the caddy.

How to Select Objectives 1. Click the Choose Objective Magnification Icon on the tool bar. 2. Click the required objective magnification.

How to Add Objectives 1. Click Display Rulers Tool Icon on the tool bar. 2. Click the Objective Calibration button. 3. Click New Objective. The magnification will be the same as the current magnification. 4. Click the required objective to edit Objective magnification and Fine Adjustment values.

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Section 5 RI Viewer ™

Research Instruments Ltd

How to Remove Objectives 1. Click Display Rulers Tool Icon on the tool bar. 2. Click the Objective Calibration button. 3. Click X next to the objective to be removed.

How to Check Objective Calibration 1. Click the Display Rulers Tool Icon on the tool bar. 2. Click the Objective Calibration button to open the Objective Calibration panel. 3. Place an object of known dimensions (a stage micrometer is supplied with each system for this purpose) in the field of view. Ensure that the objective selected in RI Viewer matches that being used on the microscope.

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4. The stage micrometer supplied measures 100µm between the longer lines. For best accuracy, position each end point of the line at exactly the same relative position on the scale, for example at the right hand edge of each vertical line. 5. Drag the rulers to the point where the stage micrometer is going to be measured from and use the fine adjustment up and down until the ruler scale matches the stage micrometer.

How to Configure the Foot Pedal/Keyboard 1. Click the General Settings Icon on the tool bar. 2. From the Settings panel click the Keyboard/Foot Pedals Shortcuts. 3. Click the corresponding edit button to assign a shortcut. 4. Hold down the key or press the foot pedal to assign. 5. Click Save and Quit. Once the shortcuts are set up, press the shortcut keys or the corresponding foot pedal to activate the command.

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