Saturn Laser System , RI IMS and RI Viewer User Manual Issue 5

CONTENTS SECTION 1

5

Preface5 SECTION 2

6

Introduction to Saturn™ Laser Systems

6

Intended Use Applicable Part Numbers Microscope Compatibility Installation

6 6 6 7

SECTION 3

8

Saturn™ Laser Systems Safety Precautions

8

Safety Precautions Guidance and Manufacturer’s Declaration – Electromagnetic Emissions (IEC 6060 1-1-2) Guidance and Manufacturer’s Declaration - Electromagnetic Immunity Guidance and Manufacturer’s Declaration - Electromagnetic Immunity User Interface Icons Symbols 

8 9 10 11 12 13 14

SECTION 4

15

Saturn™ Laser Systems Product Overview

15

Hardware Overview Saturn™ Laser System Component Part Numbers Saturn™ Laser System Mirror Module Part Number and Descriptions Saturn Active™ Mirror Modules Packaging and Handling Requirements Saturn™ Mirror Modules Laser Specifications Laser Specifications Ablation Laser Pilot Laser Objective Specifications  Electrical Specifications  Dimensions  Operating Range 

15 16 16 16 16 17 18 18 18 18 18 18 18 18

SECTION 5

19

RI Viewer™ 

19

Introduction How to View Live Images

19 19

CONTENTS How to Zoom & Pan the Image Changing Camera Settings How to Take a Picture How to Record Video How to Perform Measurements

19 19 20 20 20

SECTION 6

21

Saturn™ Laser Systems Operation

21

How to Configure the Laser for a New Procedure How to Check Objective Calibration How to Check Laser Target Alignment How to Check Hole Size Calibration How to Remove a Point from the Hole Size Calibration Graph How to Fire the Laser (Single Pulse) How to Adjust Hole Size/Pulse Width Laser Target  Exclusion Zone Hole Size Indicator  Preset Pulse Lengths How to Shut Down

21 21 21 22 22 23 23 23 23 23 24 24

SECTION 7

25

Assisted Hatching Procedure

25

Introduction Summary Ablation Procedure 

25 25 26

SECTION 8

28

Saturn™ Laser Systems Additional Modes

28

USA Clearance Regulations How to Enable Biopsy Mode How to Enable Multi-Pulse Mode How to Fire the Laser (Biopsy Mode) How to Fire the Laser (Multi-Pulse Mode)

28 28 28 28 29

SECTION 9

30

Saturn™ Laser Systems Troubleshooting

30

Problem Possible Cause Solution

30 30 30

CONTENTS SECTION 10

31

RI IMSI™

31

Introduction Specifications Hardware Overview Condenser Objectives IMSI Monitor Monitor Settings (for Medical Monitor Supplied by RI)  Sample Preparation  Observation Maintenance  Troubleshooting Problem Possible Cause Solution

31 31 31 31 31 32 32 32 32 33 34 34 34 34

SECTION 11

35

Appendices

35

How to Select Cameras Camera Flipping How to Set the Preset Zoom How to Configure the Foot Pedal/Keyboard How to View Still and Recorded Images How to Select Objectives How to Add Objectives How to Remove Objectives 

35 35 35 35 35 36 36 36

SECTION 12

37

Saturn™ Laser Systems Care And Maintenance

37

Disposal of Goods 

37

SECTION 13

38

Returns Procedure

38

Research Instruments Ltd  Customer Feedback

38 38

Section 1

Research Instruments Ltd

1

PREFACE This manual is the sole property of Research Instruments, and along with the Research Instruments products to which it applies, is provided for the exclusive use of Research Instruments clients. In no event does Research Instruments assume the liability for any technical or editorial errors of commission, or omission; nor is Research Instruments liable for direct, indirect, incidental, or consequential damages arising out of the use or inability to use this manual. © This manual is protected by copyright, all rights reserved, and no part hereof may be photocopied or reproduced in any form without the prior written consent of Research Instruments. Saturn™, RI Viewer™ and RI IMSI™ are trademarks of Research instruments Ltd. All other brand names are trademarks of their respective owners. The information in this manual is current at the time of publication. Our commitment to product improvement requires that we reserve the right to change equipment, procedures and specifications at any time. Note Assisted Hatching is not recommended for routine use in all ART patients. The system should be operated by qualified and trained personnel only.

5

Section 2

Introduction to Saturn™ Laser Systems

INTRODUCTION TO SATURN™ LASER SYSTEMS Intended Use The Saturn™ Laser System is intended ‘to ablate a User defined section of a gamete or embryo within ART’.

2

Applicable indications for use are subject to the regulations of the country into which the device is sold. Availability of a Saturn™ Laser System for clinical use is dependent on the regulatory approval status of the Saturn™ Laser System within the country the device is intended to be sold into.

0120 USA ONLY Ref: FDA 510(k) K060764 and K083208. The Saturn™ Laser System is to be used to drill a small tangential hole in, or to thin, the zona pellucida of the embryo in selected in vitro fertilization (IVF) patients with otherwise poor prognosis for successful pregnancy outcome such as: Advanced maternal age Prior failed IVF Cryopreserved embryos Abnormal zona pellucida morphology Only

Caution: Federal law restricts this device to sale by or on the order of a physician or a practitioner trained and certified in its use

Applicable Part Numbers 6-47-500 - Saturn 5 Active™ Laser 6-47-501 - Saturn 5™ Fixed Laser 6-47-510 - Saturn 5 Active™ Laser competitor replacement 6-47-511 - Saturn 5™ Fixed Laser competitor replacement 6-47-512 - Upgrade from Saturn™ to Saturn 5 Active™ 6-47-513 - Upgrade from Saturn™ to Saturn 5™ Fixed 6-47-514 - Upgrade from Saturn 3 Active™ to Saturn 5 Active™ 6-47-515 - Upgrade from Saturn 5™ Fixed to Saturn 5 Active™

Microscope Compatibility Zeiss Axiovert 40/100/200/Observer Nikon TMD, D200/300, TE200/300, TE2000, Ti Leica DMIRB, DMI3000B/4000B/6000B, DMIL Olympus IMT2, IX50/70, IX51/71/81, IX53/73/83

6

Section 2

Research Instruments Ltd

Installation Installation of the Saturn™ Laser System should be carried out by a Research Instruments technician or other RI-authorised personnel. Incorrect installation could result in reduced power output, laser misalignment or laser malfunction.

2

All relevant sections of this manual should be read and understood fully before any operation of the Saturn™ Laser System takes place. If the operator is unsure of any of the information contained in this manual, they should contact Research Instruments or an appointed representative before attempting to use this equipment. A Patients’ Guide to Laser Assisted Hatching (LAH) is provided on the RI Viewer™ installation CD (LAH Patients Guide.pdf). This can be opened using Adobe Reader or other PDF viewers. We recommend that copies are printed and made available to patients. For outside of the USA an installation manual will be supplied (6-47-500IM) No user serviceable parts.

7

Section 3

Saturn™ Laser Systems Safety Precautions

SATURN™ LASER SYSTEMS SAFETY PRECAUTIONS Safety This indicates cautionary text which should be followed to avoid injury to users or damage to samples Laser radiation is present within the control unit, the fibre optic patch lead and through the microscope to output at the objective. The Saturn™ laser system has been designed to ensure that emission of laser radiation at any point is within the limits stipulated by international safety standards, IEC60825-1:2008, and US 21CFR1040.10 for unprotected viewing (Class 1). As an extra precaution, an infrared blocking filter has been incorporated into the optical path leading to the eyepieces to reduce laser emission by a further 99%.

3

CAUTION hazardous radiation exposure may occur through the use of controls, executing adjustments or the performance of procedures other than those specified herein. Servicing and adjustment other than that specified in this manual should only be carried out by RI or RI authorised agents. DO NOT disassemble or modify any part of the Saturn™ Laser system, or substitute any component for any other. Doing so may result in exposure to dangerous levels of laser radiation. This voids the warranty and/or service contract. DO NOT use a magnifying glass or eye loupe above the objective lens. DO NOT operate the laser unless it is properly mounted to a microscope. ONLY use the power cable and power supply adaptor supplied with the system. The cable to the power supply is the Disconnect Device for this equipment. To remove all electrical power from this product, disconnect the power cable from the electrical outlet. Equipment should be positioned so as to allow easy access to the power cable. The appliance coupler or mains plug is used as the disconnect and must remain readily operable. WARNING To avoid the risk of electric shock, this equipment must only be connected to a supply mains with protective earth.

WARNING Not to be used in a patient environment

WARNING Before performing any procedure, the pilot laser must be used to check the alignment of the Active positioning system. The system must be re-aligned when necessary. See Section 6 - How to Check Laser Target Alignment.

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Section 3

Research Instruments Ltd

WARNING Thermal lensing is a defocusing of the laser beam caused by changes to the refractive index of the medium as it is heated. The degree of defocus depends on the total beam energy deposited in the medium. While slight defocus has no effect on the drilling properties, this effect may become a problem for higher beam energies. To eliminate any significant effects from thermal lensing, we recommend that the pulse length be kept below 1000μs for clinical Laser Assisted Hatching (LAH) zona drilling. WARNING Only a single opening should be made in the zona pellucida. Multiple openings or those that are too small may prevent embryo hatching or lead to abnormal development.

3

WARNING Do not operate the pilot laser with an embryo in the field of view. Exposure to the pilot laser may damage the embryo. WARNING The microscope itself must be maintained to a high standard. Problems such as worn focus mechanisms or an insecure video camera may lead to unreliable focus and image stability, and could lead to embryo damage. WARNING Do not operate the laser without the RI Viewer™ software running, as targeting and hole size indication will not be active, and the embryo may be damaged. WARNING The embryo may be damaged if objectives other than the red Saturn™ laser objective is used.

WARNING Do not modify this equipment without authorization of the manufacturer. WARNING Where an ITO glass heated stage is fitted, do not fire the laser with the microscope focussed into the ITO glass surface as this may damage the ITO coating.

Precautions •

To minimise the risk of damage to blastomeres, administer as few laser pulses as possible at the lowest energy levels possible to achieve the prescribed zona drilling or thinning effect.

•

Direct the laser beam toward a section of the zona pellucida where the adjacent perivitelline space is widest or next to an area of fragmentation.

•

A holding pipette should be used during laser treatment to minimize the risk of embryo movement.

•

Small openings in the zona pellucida may lead to embryo constriction and abnormal development.

•

To date there are no known reports showing a greater occurrence rate of major or minor defects in children derived from laser-hatched embryos. Long-term follow-up data on children born from laser-hatched embryos does not yet exist. A study of 134 such babies* found no increase in the major congenital malformations, chromosomal aberrations or minor congenital malformations between the LAH treated group and all deliveries at their hospital.

•

The device is not affected by and does not present any reciprocal interference to the microscope.

•

During normal use there is no contact with patient/sample. Standard lab cleaning procedures can be applied. *Kanyo, K., Konc, J. “A follow-up study of children born after diode laser assisted hatching.” European Journal of Obstetrics and Gynaecology. 110: 176-180 (2003).

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Section 3

Saturn™ Laser Systems Safety Precautions

Guidance and Manufacturer’s Declaration – Electromagnetic Emissions (IEC 6060 1-1-2) The Saturn™ Laser System is intended for use in the electromagnetic environment specified below. The customer or the user of the Saturn™ Laser System should assure that it is used in such an environment. Emissions test

3

RF emissions CISPR 11

RF emissions CISPR 11

Harmonic emissions IEC 61000-3-2 Voltage fluctuations/flicker emissions

10

Compliance

Group 1

Class B

Class A Complies

Electromagnetic environment guidance The Saturn™ Laser System uses RF energy only for its internal function. Therefore, its RF emissions are very low and are not likely to cause any interference in nearby electronic equipment. The Saturn™ Laser System is suitable for use in all establishments, including The Saturn™ Laser System domestic establishments and is suitable for use in all those directly connected to establishments, including the public low-voltage power domestic establishments and supply network that supplies those directly connected to buildings used for domestic the public low-voltage power purposes. supply network that supplies buildings used for domestic purposes.

Section 3

Research Instruments Ltd

Guidance and Manufacturer’s Declaration - Electromagnetic Immunity The Saturn™ Laser System is intended for use in the electromagnetic environment specified below. The customer or the user of the Saturn™ Laser System should ensure that it is used in such an environment. IMMUNITY test

IEC 60601 Test level

Compliance level

Electro magnetic environment guidance

Electrostatic discharge (ESD) IEC 61000-4-2

± 6 kV contact ± 8 kV air

± 6 kV contact ± 8 kV air

Floors should be wood, concrete or ceramic tile. If floors are covered with synthetic material, the relative humidity should be at least 30 %.

Electrical fast transient/burst IEC 61000-4-4

± 2 kV for power supply lines ± 1 kV for input/ output lines

± 2 kV for power supply lines ± 1 kV for input/ output Lines

Mains power quality should be that of a typical commercial or hospital environment.

Surge IEC 61000-4-5

± 1 kV line(s) to ± 1 kV differential line(s) Mode ± 2 kV line(s) to earth ± 2 kV common mode

Mains power quality should be that of a typical commercial or hospital environment.

Voltage dips, short interruptions and voltage variations on power supply input lines IEC 61000-4-11

<5 % UT (>95 % dip in UT ) for 0.5 cycle 40 % UT (60 % dip in UT) for 5 cycles 70 % UT (30 % dip in UT) for 25 cycles <5 % UT (>95 % dip in UT) for 5s

<5 % UT (>95 % dip in UT) for 0.5 cycle 40 % UT (60 % dip in UT) for 5 cycles 70 % UT (30 % dip in UT) for 25 cycles <5 % UT (>95 % dip in UT) for 5s

Mains power quality should be that of a typical commercial or hospital environment. If the user of the Model 004 image intensifier requires continued operation during power mains interruptions, it is recommended that the Model 004 Image Intensifier be powered from an uninterruptible power supply or a battery.

Power frequency (50/60 Hz) magnetic field

3 A/m

0.3 A/m

If image distortion occurs, it may be necessary to position the Model 004 image intensifier further from sources of power frequency magnetic fields or to install magnetic shielding. The power frequency magnetic field should be measured in the intended installation location to assure that it is sufficiently low.

IEC 61000-4-8

3

Note: UT is the a.c. mains voltage prior to application of the test level.

11

Section 3

Saturn™ Laser Systems Safety Precautions

Guidance and Manufacturer’s Declaration - Electromagnetic Immunity The Saturn™ Laser System is intended for use in the electromagnetic environment specified below. The customer or the user of the Saturn™ Laser System should assure that it is used in such an environment.

3

Conducted RF

3 Vrms

IEC 61000-4-6

150 kHz to 80 MHz

Radiated RF

3 V/m

IEC 61000-4-3

80 MHz to 2.5 GHz

3 Vrms

3 V/m

Portable and mobile RF communications equipment should be used no closer to any part of the Model 006, including cables, than the recommended separation distance calculated from the equation applicable to the frequency of the transmitter. Recommended separation distance d = 1,2√P d = 1,2√P 80 MHz to 800 MHz d = 2,3√P 800 MHz to 2 , 5 GHz where P is the maximum output power rating of the transmitter in watts (W) according to the transmitter manufacturer and d is the recommended separation distance in metres (m). Field strengths from fixed RF transmitters, as determined by an electromagnetic site survey, a should be less than the compliance level in each frequency range. b Interference may occur in the vicinity of equipment marked with the following symbol:

Note 1: At 80 MHz and 800 MHz, the higher frequency range applies. Note 2: These guidelines may not apply in all situations. Electromagnetic propagation is affected by absorption and reflection from structures, objects and people. Field strengths from fixed transmitters, such as base stations for radio (cellular/cordless) telephone and land mobile radios, amateur radio, AM and FM radio broadcast and TV broadcast cannot be predicted theoretically with accuracy. To assess the electromagnetic environment due to fixed RF transmitters, an electromagnetic site survey should be considered. If the measured field strength in the location in which the Model 006 is used exceeds the applicable RF compliance level above, the Model 006 should be observed to verify normal operation. If abnormal performance is observed, additional measures may be necessary, such as re-orienting or relocating the Model 006. b Over the frequency range 150 kHz to 80 MHz, field strengths should be less than 3 V/m. a

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Section 3

Research Instruments Ltd

User Interface Icons Symbol

Meaning

Symbol

Meaning

Take a picture

Laser control

Take a video

General settings

Stop video recording

Additional camera settings

View images

Take measurements

Choose objective magnification

Display rulers tool

Increase/Decrease zoom level

Instructions For Use

3

13

Section 3

Saturn™ Laser Systems Safety Precautions

Symbols Symbol

Meaning

Only

3

Caution: Federal law restricts this device to sale by or on the order of a (licensed healthcare practitioner).

Indicates disposal of goods instruction

Class 1 laser product

0120

In accordance with European Medical Device Directive 93/42/EEC

Indicates the medical device manufacturer, as defined in EU Directives 90/385/EEC, 93/42/EEC and 98/79/EC. Indicates the need for the user to consult the instructions for use for important cautionary information such as warnings and precautions that cannot, for a variety of reasons, be presented on the medical device itself. Important safety instructions

SN

14

The first four digits are a unique identifier assigned to the control unit and the last 2 digits signify the year of manufacture, e.g. 5001/13 (this denotes a unique serial number of 5001 and a year of manufacture 2013).

PILOT

0120

MANUFACTURED IN UNITED KINGDOM

QC

Saturn Laser System Research Instruments Ltd www.research-instruments.com

PC

LASER OUTPUT KEEP CLEAN

USE AC ADAPTOR 1-10-100

Section 4

Research Instruments Ltd

SATURN™ LASER SYSTEMS PRODUCT OVERVIEW Hardware Overview Rear Panel 5-47-010

CONSULT ACCOMPANYING DOCUMENTS

PILOT

0120

MANUFACTURED IN UNITED KINGDOM

QC

CLASS 1 LASER PRODUCT

Saturn Laser System Research Instruments Ltd www.research-instruments.com

PC

12V

2.5A

LASER OUTPUT KEEP CLEAN

USE AC ADAPTOR 1-10-100

Pilot laser brightness: The brightness of the pilot laser can be adjusted by turning the control to obtain the clearest image in the target alignment procedure.

4

The light on the front of the control unit indicates that the control unit is operational. If the unit is plugged in PART NUMBER: 2-47-012 SATURN REAR PANEL ARTWORK and the light is not on, contact Research Instruments for assistance. ECN: 1002 ISSUE 2 DRAWN: RGM System Components DATE:15/10/12

Power Supply Mirror Module

Control Unit Objective

Motor Module

Patch Lead Note Actual components may differ from those shown. The Saturn™ Laser System comes supplied with the following parts a control unit containing the lasers, mirror module, armoured patch lead and a special objective. If you have a Saturn Active™ system then an additional motor module will be supplied. The laser beams are delivered to the specimen through a fibre optic patch lead, collimator module (that is part of the mirror module for a fixed system or the motor module for an active system) and the special objective. A foot pedal for firing the laser is available as an optional extra, this is attached to the PC by a mini USB cable. The motor module receives coordinates from USB which moves the motors to those coordinates, the control unit then receives commands from USB to fire for a specifies length of time.

15

Section 4

Saturn™ Laser Systems Product Overview

Packaging and Handling Requirements No special packaging or handling requirements

Saturn™ Laser System Component Part Numbers Part Number 5-47-010 5-47-100 1-10-100 2-45-801

4

Description Control Unit with 1.5m Patch Lead Motor Module (Saturn™ Active) 12V 2.5A Medical PSU (x2 for Saturn Active™) Laser Objective (red)

Saturn™ Laser System Mirror Module Part Number and Descriptions Saturn Active™ Mirror Modules Part Number 5-46-163 5-46-150 5-46-160 5-46-151 5-46-152 5-46-159 5-46-135 5-46-132 5-46-130 5-46-140 5-46-141 5-46-142 5-46-144 5-46-158 5-46-110 5-46-120 5-46-122 5-46-121

16

Description Mirror module for IX53/73/83 left Mirror module for IX70 left Mirror module for IX70 right Mirror module for IX71 left Mirror module for IX71 right Mirror module for IMT2 Mirror module for TMD Mirror module for D300 Mirror module for TE300 Mirror module for TE2000 with Integra Ti™ Mirror module for TE2000 without Integra Ti™ Mirror module for Nikon Ti with Integra Ti™ Mirror module for Nikon Ti with X-Y Mirror module for Ax40 Mirror module for Ax200/Observer Mirror module for DMIRB Mirror module for DMI3000B Mirror module for DMI4000B/6000B

Section 4

Research Instruments Ltd

Saturn™ Mirror Modules 5-45-163 5-45-383 5-45-387 5-45-388 5-45-580 5-45-411 5-45-301 5-45-545 5-45-546 5-45-575 5-45-009 5-45-011 5-45-450 5-45-470 5-45-461 5-45-460

Collimator module for IX53/73/83 Collimator module for IX70 Collimator module for IX71 Collimator module for IX71 fluor Collimator module for IMT2 Collimator module for TMD Collimator module for D300/TE300 Collimator module for TE2000/Nikon Ti Collimator module for TE2000/Nikon Ti fluor Collimator module for Ax40 Collimator module for Ax100 Collimator module for Ax200/Observer Collimator module for DMIRB Collimator module for DMIL Collimator module for DMI3000B Collimator module for DMI4000B/6000B

4

17

Section 4

Saturn™ Laser Systems Product Overview

Laser Specifications Laser Specifications Output wavelength Power output from patch lead Maximum pulse length *Laser Safety Classification

Ablation Laser 1480nm 400mW 2.0ms Class 1

Pilot Laser 650nm 130µW (max) None Class 1

* IEC60825-1: 2008, US 21CFR 1040.10

Objective Specifications

4

Magnification N.A. Working distance Parfocal distance

40x 0.49 2.5mm 45mm (adaptor supplied for Nikon CFI60 systems)

Electrical Specifications Mains Adaptor for Control Unit and Motor Module Input Output Power (maximum) Voltage Current (maximum) Earth Connection

100-240VAC, 50-60Hz, 0.8-0.4A 30W 12VDC 2.50A Mandatory 3-pin plug for earthing (grounding)

Dimensions Control unit Weight

220mmx180mmx34mm (8.6”x7.1”x1.3”) 1.2Kg

Operating Range Temperature Humidity

10°C (50°F) to 42°C (108°F) 15% to 85% RH (Non Condensing)

USA Only Compliance with the emissions requirements of CISPR 22 Class A requires the following warning: “This is a class A product. In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures.”

18

Section 5

Research Instruments Ltd

RI VIEWER™ Introduction RI Viewer™ is the software that interacts with the Saturn™ and RI IMSI™ range of products from Research Instruments for use within an ART laboratory. Connected to a camera attached to a microscope, it provides on screen images of samples under the microscope. It can record video and store still images from the microscope in a PC’s file system. Supplied PC’s are IEC 60950-1 approved. The operator should be positioned in such a place to easily access the microscope and also view the image on the PC monitor.

How to View Live Images A camera will need to be connected to the PC running RI Viewer™ and the drivers installed correctly to view live images within RI Viewer™. The dongle must also be fitted to a USB port. RI Viewer™ software will automatically recognise both analogue and compatible digital camera devices connected to the PC. If there is only one camera device detected, it will show the live image for that device. If multiple camera devices are connected it will use the camera that was last selected from the Video Source drop down box.

5

If there are multiple cameras attached to the PC, you may select the device you want the live image to be viewed from. To do this select the camera from the dropdown list on the Settings panel. See Section 11 - How to Select Cameras.

How to Zoom & Pan the Image There are three ways to zoom into an area on the screen. 1. Place the mouse at a point on the screen and click and hold the right mouse button then release. This will zoom into the spot where the mouse is placed. To zoom out, click and hold the right mouse button. 2. Place the mouse at a point on the screen and use the scroll wheel on the mouse (if available).This will give the user control over how much digital zoom is given. 3. Using the tool bar at the bottom of the screen, click the + icon to zoom in incrementally. To zoom out by the same amount, click on the - icon on the tool bar. The magnifying glass icon will zoom in and out by the preset zoom. Whilst using digital zoom, a thumbnail image of the screen will appear in the top left of the screen. At the top of this panel is the amount of magnification you are using. See Section 11 - How to Set the Preset Zoom. When zoomed out fully, this panel will not appear on the screen. Zooming in and zooming out can also be achieved using the foot pedal. See Section 11 - How to Configure Foot Pedal/Keyboard.

Changing Camera Settings Clicking on the tool bar will show a panel indicating the camera name and frames per second (fps). Clicking on Additional Settings will allow the user to adjust settings on the video source. Only settings available to the video source chosen will appear.

19

Section 5 RI Viewer ™

How to Take a Picture 1. Click

on the tool bar.

2. A camera icon will flash briefly in the centre of the screen to let the user know that an image has been taken. Images are stored in a .bmp file format. Images are named by default (date and time) yyyymmddhhmmss.bmp. For example, a picture taken on the 3rd of January 2012 at 10:35:02 would be named 20120103103502.bmp.

How to Record Video 1. Click

on the tool bar.

2. Whilst video is being captured, a recording notification will flash in the top left corner of the screen.

5

3. Click

on the tool bar and the recording notification will stop.

4. Each video recording can last for a maximum of one hour. This eliminates the problem of accidentally leaving the software recording with the possibility of rendering the computer unusable. Recorded videos are stored in a .wmv file format Recorded videos are named by default (date and time) yyyymmddhhmmss.wmv. For example, a video taken on the 3rd of January 2012 at 10:35:02 would be named 20120103103502.wmv.

How to Perform Measurements 1. Click

on the tool bar.

2. Click on the screen to select a start point for the measurement. 3. Click on the screen a second time to select an end point for the measurement. The ends of the line can be dragged to change the measurement. Lines can be removed by clicking the cross in the context window. Lines are shown during laser operation but cannot be dragged/created and no measurements will be displayed. Lines and measurements are shown on an image when images are taken in line mode.

20