MISTRAL 3000i Instruction Manual Feb 2006

SANYO GALLENKAMP PLC Health and Safety at Work Sanyo Gallenkamp is required under the Health and Safety at work, etc. Act, 1974 and other UK legislation as designers, manufacturers, suppliers and importers of articles for use at work to ensure that, as far as is reasonably practicable, articles which we design, produce, supply or import are safe and without risk to health. We are required to provide information on the safety and handling precautions to be observed when installing, operating, maintaining and servicing our products. Such advice is contained in this manual. We are also obliged to update this information should circumstances change and to operate a system to this end. We should also like to point out, however that you as users have an important responsibility in the provision and maintenance of safe working practices and conditions. Accordingly, we draw the following matters to your attention: 1.

This apparatus should only be used as intended and within its design parameters by suitably qualified and trained personnel who have read and understood the relevant sections of this manual.

2.

This manual should be readily available to such personnel at all times.

3.

In addition to that which is written in the manual, normal common-sense safety precautions must be taken at all times to avoid the possibility of accidents. Particular care is required when working with apparatus at high temperature or pressure.

4.

Installation, maintenance, repairs and servicing should only be carried out by an SANYO Gallenkamp approved engineer, and connection to electrical supplies should only be carried out by suitably trained personnel.

TECHNICAL SUPPORT, WARRANTY SERVICE AND MAINTENANCE UK customers; if you are in any doubt whatsoever regarding the correct use of this apparatus, or if you require any technical data or assistance, please contact the SANYO Gallenkamp Technical Support Department at; SANYO GALLENKAMP PLC

Monarch Way Belton Park Loughborough Leicester LE11 5XG

Telephone Fax E-mail: Web Page:

+44 (0) 1509 265265 +44 (0) 1509 269770 technical.support@sgplc.co.uk www.sanyogallenkamp.com

OVERSEAS CUSTOMERS: Should contact their local Sanyo Gallenkamp Distributor, details can be found on our website. ELECTRICITY SUPPLIES: Voltage and frequency Sanyo Gallenkamp electrical apparatus is offered and labelled for one, or for a choice of two or more voltage ranges and, where necessary, different frequencies of mains supply. Sanyo Gallenkamp does not accept any responsibility for the operation of any such apparatus should it be connected to electricity supplies which are normally outside, or vary outside, the stated voltage and frequency values for which it is designed, nor for any consequential loss, damage or injury, howsoever caused.

Read This Before Use!

SANYO Gallenkamp PLC Monarch Way, Belton Park, Loughborough, Leicestershire, LE11 5XG. U.K. Tel: +44 (0) 1509 265265 Fax: +44 (0) 1509 269770 E-Mail: sanyogall@sgplc.co.uk Web: www.sanyogallenkamp.com

DECLARATION OF CONFORMITY according to EN 45 014 - ( BS 7514 ) Document no: 71100.1254.1

SANYO Gallenkamp PLC. Registered Office: Monarch Way, Loughborough, Leicestershire LE11 5XG. Registered in England and Wales No. 2462454

Thank you for buying a MSE Centrifuge. Please read this operating manual before using your centrifuge; it will provide you and your colleagues with useful information on all aspects of the equipment. As our customer, we should like to ensure that you are totally satisfied at all times. Do not hesitate to contact: Sanyo Gallenkamp - your views are very important to us.

Sanyo Gallenkamp plc

ELECTRICITY SUPPLY Before connecting this apparatus to the electricity supply, check the information given on the rating plate and ensure that; A) Your supply is single phase a/c. (alternating current) of the stated frequency with neutral nominally at earth potential. B) Your supply voltage is within the stated range. C) The current rating is within the capacity of your outlet. D) Your plug or electricity supply circuit is fitted with a suitable fuse. Fuse rating

15 amp

WARNING! This apparatus must be earthed. The wires in the mains lead are coloured in accordance with the following code;

Live Neutral Earth

230v Brown Blue Green and Yellow

120v Black White Green and yellow

Green and Yellow or Green to terminal marked E (Earth), G (Ground), coloured Green or Green and Yellow or marked with the Earth symbol. Blue or white wire to terminal marked N (Neutral) or Common or coloured Blue. Brown or black wire to terminal marked L (Live) or Phase or coloured brown.

SANYO GALLENKAMP PLC PRODUCT WARRANTY Terms and Conditions We hope that you do not have the need to use the extensive warranty cover that Sanyo Gallenkamp Plc. extend to you. However should you have a problem, our prompt response is greatly helped if you have filled in and posted the pre-paid Warranty Registration Card supplied with your new equipment. Sanyo Gallenkamp Plc. give a one year warranty from the date of delivery. During this period, component parts proven to be defective in materials or workmanship will be repaired or replaced at our expense. Installation, commissioning and calibration are not covered by this warranty agreement. The Sanyo Gallenkamp Plc. approved service agent must be contacted for warranty determination and direction prior to any work being carried out. These warranties are only applicable to new products, and not second hand nor refurbished products even if repaired by Sanyo Gallenkamp Plc. Any such products are covered by separate warranty terms and conditions which will be made available on request. Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the equipment or the component part beyond the original one year warranty period unless agreed in writing by Sanyo Gallenkamp Plc. The above warranties are extended to the original purchaser upon full invoice payment. A purchase receipt or other proof of purchase may be required before warranty service will be performed. These warranties only cover failures due to defective workmanship which occur during the normal operation of the product by the original purchaser, and not failures which result from accident, misuse, abuse, neglect, mishandling, misapplication, alteration, faulty installation, electrical power fluctuations, dust, or other environmental extremes, modification or service other than by an approved service agent or following the written authority of the manufacturer, or damage that is attributable to acts of God. Expendable items such as motor brushes, door seals, lid seals, "O" rings or lamps are excluded. Sanyo Gallenkamp Plc., or its approved service agent, reserves the right to repair defective equipment on the premises of the customer, or at a service station, at the sole discretion of Sanyo Gallenkamp Plc. or their approved agent. In the event of return to an approved service centre the customer is responsible for the safe packaging of the instrument and notification to the service centre. Neither Sanyo Gallenkamp Plc. nor its agents are responsible for any damage occurring during shipment. Specification and Material Changes:- Sanyo Gallenkamp Plc. reserves the right to supply our latest and improved models at time of shipment. Taxes:- The prices quoted do not include any taxes imposed by the State or Country in which the purchase was made. Installation:- Installation of all equipment shall be by, and at the expense of the purchaser unless stated otherwise. Access to the site, and the provision of required utilities e.g. Power, water and drainage to suitable connections, will be the responsibility of the purchaser, and at the purchaser's expense. Limitation of liability:- In no event, whether as a result of breach of contract or warranty, shall Sanyo Gallenkamp Plc. be liable for any consequential or incidental damages including, but not limited to, loss of profit or revenues, loss of use of the equipment or any associated equipment, down time costs, costs of substitute equipment, costs of labour, costs due to delays or claims of purchaser's own customers for such damages. The purchaser agrees to indemnify Sanyo Gallenkamp Plc. and to hold them harmless from any and all liability, claims, demands, actions, suits, expenses or costs, including attorney's fees relating to such consequential or incidental damages. All expressed and implied warranties, including the warranties of merchantable quality and fitness for a particular purpose, are limited to the application period of one year. Validity:- Legal rights vary from country to country and states within countries, so some or all of the exclusions or limitations listed above may not apply, but if any part of these conditions shall be found to be unenforceable it shall not affect the validity or enforceability of the remainder of the conditions.

CONTENTS

8 TABLE OF CONTENTS

1.

GENERAL INFORMATION ... 10

2.

GENERAL DESCRIPTION... 13 2.1 2.2

3.

INSTALLATION ... 15 3.1 3.2 3.3 3.4

4.

Critical System Errors ... 27 Uncritical System Errors ... 30

ROTORS... 33 8.1 8.2 8.3 8.4 8.5 8.6

9.

How to enter a new program ... 25 How to copy an existing program... 26

EXPLANATION OF ERROR MESSAGES ... 27 7.1 7.2

8.

Setting run value ... 21 Run procedure... 21 Examination of set values ... 22 Changing set values ... 22 Changing from time to time hold ... 22 Changing between SPEED and RCF modes ... 22 Precool facility... 23 Programmes ... 23 Saving a programme ... 24 Recalling a Stored Programme ... 24 Stop procedure... 24 Power failure ... 24 Opening lid during power failure ... 24

PROGRAMMING EXAMPLES ... 25 6.1 6.2

7.

Set Keys ... 17 Operation Keys ... 19

OPERATION... 21 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 5.10 5.11 5.12 5.13

6.

Mounting ... 15 Forces acting on fixings ... 15 Connecting power supply ... 15 Accessories ... 15

THE CONTROL PANEL... 16 4.1 4.2

5.

Centrifuge Specification ... 14 Independent Speed Sensing - 120 volt machines only ... 14

Installation of Rotor ... 33 Removal of Rotor ... 33 Loading the Rotor ... 33 Balancing the rotor ... 34 Critical Speeds ... 34 Rotor Speed ... 34

ROTOR AND ACCESSORY TABLES ... 35

CONTENTS 9.1 9.2 10.

Table 1 Swing-out rotors ... 36 Table 2 Angle rotors ... 38

MAINTENANCE ... 39 10.1 10.2 10.3 10.4 10.5 10.6 10.7 10.8

11.

9

Cleaning ... 39 Disinfecting... 39 Drive shaft... 40 Rotors and buckets... 40 O-Rings. ... 40 Sterilisation. ... 40 Lid seal. ... 40 Detection of Corrosion ... 40

SERVICE INSTRUCTIONS ... 41

GENERAL INFORMATION 1.

10

GENERAL INFORMATION PLEASE READ THIS MANUAL BEFORE OPERATING THE CENTRIFUGE The following general instructions, and the precautions regarding the prevention of corrosion in rotors and accessories, as described under MAINTENANCE, must be noted and carefully observed. The maximum load should always be checked before commencing any operation. If the sample has a relative density greater than 1.2, the maximum permissible speed of the rotor should be calculated as given in this publication, and applied. Any liquid spilt in the centrifuge should be removed immediately because corrosion is a major cause of failure in centrifuges. Where particularly corrosive solutions are used, the samples should be placed in sealed containers and all necessary precautions observed. The following list gives some examples of corrosive liquids used, particularly in the biochemical field: Phenol/cresol/water, chloroform/isoamyl alcohol, salt solutions (especially ammonium sulphate), solutions of ammonium hydroxide and solutions of acids such as hydrochloric, trichloroacetic and perchloric acids. It should be noted that many other substances used in different fields are often equally corrosive. Users should always check whether or not a substance being used is corrosive in order to take suitable precautions. The load must be evenly distributed symmetrically round the rotating assembly so that it is evenly balanced and complies with the manufacturer’s instructions in this respect. After use the sleeves and adaptors in centrifuge rotors should be removed to allow thorough cleaning of rotors and accessories and so prevent corrosion. Special care is necessary when using infective material in centrifuges. Containers when used with such materials, should be closed while being centrifuged and sterilised immediately after use, using non-corrosive means. Care should be taken to preserve intact, accessories which have been supplied as sets, in which all the members possess closely similar weights. It is advisable to have the centrifuge checked by a competent engineer, preferably the manufacturer’s representative, at least once per year.

GENERAL INFORMATION

Figure 1 Front view of Mistral 3000i

11

GENERAL INFORMATION

12

Figure 2 Rear view of Mistral 3000i

GENERAL DESCRIPTION 2.

13

GENERAL DESCRIPTION The Mistral 3000i is a compact, large capacity, bench-top centrifuge with brushless motor, and incorporates microprocessor control, the functions of which are programmable from the key pad on the display panel. In the event of a malfunction a diagnostic indication will be displayed and the rotor is automatically allowed to come to rest. The unit is designed to comply with BS 4402-1982 - Safety Requirements of Laboratory Centrifuges. The M3000i centrifuge is CE marked in line with European Directives 89/336/EEC Electromagnetic Compatibility, see “Declaration of Conformity” for standard used. This centrifuge is designed to comply with the following standards:BS EN 61010-1 : 1993

Safety requirements for electrical equipment for measurement, control and laboratory use.

BS EN 61010-2-020 : 1995

Particular requirements for laboratory centrifuges.

GENERAL DESCRIPTION 2.1

Centrifuge Specification Dimensions: Height Width Depth Weight

14

383mm (15.1 ins) 826mm (32.5 ins) 600mm (23.6ins) 106kg (234 lbs)

Power Supply:

230 volts, single phase, 50 Hz (see Ratings Label) 120 volts, single phase, 60 Hz

Standard Operating Conditions:

Atmospheric Pressure: Ambient Temp.: Max. Operating Temp.: Heat Output: Noise Output:

Speed Range: Temperature Control:

200 to 6000 rev/min to an accuracy of 10 rev/min of set speed

Control range: 0C to 40C with an accuracy of ±2C of the set temperature. Selectable range: -9C to 40C

Timing Setting:

< 1 hour : 1 second to 59 minutes:59 seconds > 1 hour : 1 hour to 99 hours:59 minutes continuous in time hold

Programmes:

50 programmes can be stored in memory to save commonly used run data. 0 - 6003

RCF Range:

2.2

950-1015 mbar (13.78-14.71 psi) 10C to 30C 35C 1.5 kW approx. 64 dba at 1 metre

Independent Speed Sensing - 120 volt machines only To comply with certain USA standards the 120 volt machines are fitted with a small window through the cover. This permits independent speed calibration directly from the rotor with the use of a suitable stroboscopic light source. Note: Machines fitted with this window do not comply with the requirements laid down in BS 4402.

INSTALLATION 3. 3.1

15

INSTALLATION In order to install the Mistral 3000i these procedures should be followed. Mounting Before operating this machine it is essential that it is sited on a continuous level and solid surface, with all feet firmly on the bench. There must be no access to the underside of the centrifuge. Provision is made to allow securing to the surface. When mounting on a bench, it is important that at least 10 cm clearance is allowed at the sides and rear of the centrifuge when in its operating position.

3.2

Forces acting on fixings The maximum forces acting upon fixings when the centrifuge is secured to the working surface are: Rotational: 7,2000 Nm (5,310 lbf ft) Translation: 14,450 N (3,250 lbf)

3.3

Connecting power supply 230 volt Connect the 3 core to a suitable 3 pin plug fitted with a 10 amp fuse as follows: Brown wire to live (L) terminal Blue wire to neutral (N) terminal Green/yellow wire to earth (E) terminal 120 volt The centrifuge will have a power cable/moulded plug already fitted for 120 v supply. Black wire to live (L) White wire to neutral (N) Green/yellow wire to earth (E) Connect the centrifuge to the power supply and press the power switch on. The “POWER ON” and the “LID UNLOCKED” indicators will come on. Push the lid lock actuator to the right and open the lid. Remove transit packing from the centrifuge drive shaft if fitted.

3.4

Accessories The following accessories are supplied with the centrifuge: Description Quantity Fuse : 2 Lid Lock release key : 1 Operating manual : 1 Lifting handle : 2 M.8 screws x 25 mm long : 4 Rotor spanner : 1

THE CONTROL PANEL 4.

16

THE CONTROL PANEL The keypad has keys for setting variables, entering numbers and initiating functions. The main control panel keys are itemised below:1.Set Speed 2.Set Temperature 3.Set Time 4.Set Acceleration Rate 5.Set Brake Rate 6.Display RCF 7.Precool 8.Time Hold 9.Start 10.Stop 11.Program Copy 12.Program Recall

13.Cancel Entry 14.Special Key Functions 15.Refrigeration In Progress Indicator 16.Numeric Keys 0 to 9 17.Acceleration Display 18.Brake Rate Display 19.Status Display 20.Program Number Display 21.Time Hold In Progress Indicator 22.Speed/RCF Display 23.Temperature Display 24.Time Display

Figure 3 Main Control Panel

THE CONTROL PANEL

17

The function of each key on the main control panel and their associated displays are described below:4.1

Set Keys The set function keys are used to change the set values of speed, RCF, temperature, time, acceleration rate and brake rate. The set keys all work in the same manner. When the set key is pressed the set mode for that variable is entered and the appropriate display will go blank except for a flashing underscore in the least significant digit. The required value must then be entered using the number keys which will replace the underscore and scroll right to left as each digit is entered. The number will continue to flash until the set key is pressed again to finalise the change. The parameters and displays that can be changed by the set keys are described below. NOTE: Set keys have to be pressed again to set. Set Speed

The speed is displayed on a five digit display showing rotor speed in revolutions per minute to 10 rev/min. This display will show the set speed when the rotor is stationary and the actual speed when the rotor is running. If the set speed key is pressed during a run it will display the set speed for 5 seconds then automatically revert back to the actual speed. Set Temperature

The temperature is displayed on a two digit display showing the bowl temperature in degrees centigrade over a range of -9C to +40C. If the set temperature key is pressed when the refrigeration process is on it will display the set temperature for 5 seconds then automatically revert back to the actual temperature.

THE CONTROL PANEL

18

Set Time

When the machine is running the time is displayed on a four digit display showing the time in either hours and minutes, when the time to be displayed is greater than, or equal to 1 hour, or minutes and seconds when the time to be displayed is less than an hour. When the machine is in stationary mode the time display will be hours:minutes. Set RCF

The RCF is displayed on a five digit display, showing the set value when the rotor is stationery, and the actual value when the rotor is running. If the set RCF key is pressed during a run it will display the set RCF value for 5 seconds and then automatically revert back to the actual RCF value. The actual value can only be displayed when the rotor has been identified which is during a run when the speed is greater than 250 rev/min. Set Acceleration and Brake Rate There are two single digit displays one for the acceleration rate and one for the brake rate. The available rates are 0 to 9.

THE CONTROL PANEL

4.2

19

Operation Keys The operation keys are those used to make the unit perform one of the following functions :- Precool, Time hold, Start, Stop, Program Copy, Program recall, Cancel entry. The individual functions of the operation keys and their associated displays are described below:Precool

The precool key turns on the refrigeration when the rotor is stationary so that the bowl can be brought to the set temperature before a run. Time hold

The time hold key puts the unit into time hold mode when the rotor can be started and will run until the operator presses the stop button. The time hold indicator will illuminate to show this mode is active. The time display will count up from zero. Start

The start key is used to start a run. Stop

The stop key is used to stop a run, or truncate and save a program.

Program Copy

An existing program can be copied from one program number to another by pressing the copy program key followed by the new program number where you would like to put the copy.

THE CONTROL PANEL

20

Program Recall

The program recall function is used to call up an existing program for use. When the key is pressed the program display goes blank with a flashing underscore in the least significant digit. Press the program number required, and then press again to enter the program. Cancel Entry

The cancel entry key is used to cancel an error, mute the bleeper or clear a display in the set mode so that a new value can be entered. Special Function Keys Keypad Inhibit Pressing the decimal point followed by the 3 digit number code 1,2,3 will inhibit all keypad functions with the exception of STOP when the rotor is running, and PROG RECALL, START and PRECOOL when the rotor is stationary. To cancel press decimal point and 1,2,3 again. Rotor Run Timer This timer keeps a record of the number of hours the rotor has been running. The time is accessed by pressing the decimal point key followed by the numbers 4,5 6, this will be displayed until it is cancalled by pressing and then 4 5 6 again.

Status Display The Status Display is used to communicate the current state of the machine to the user. The display gives the highest priority message information at the time. The system errors have the highest priority followed by the operator errors, then the system status information.

OPERATION

21

5.

OPERATION

5.1

Setting run value Press

or

Enter the required value for the function selected using the numeric keypad. Repeat above until all run values required have been accepted. If a timed run is not required press

The following table shows the digit display range. Max. Digits

5.2

Speed Temperature Time

4 2 3

RCF Brake

4 1

Accel Programme

1 1

Allowed range 200 to 6,000 rev/min -9ºC to 40ºC 1 second to 59 minutes 59 seconds or 1 minute to 99 hours 59 minutes 10 to 6000g 0 to 9 0 (min. brake) 9 (max. brake) 0 to 9 0-49

Run procedure Ensure that the rotor is correctly loaded. Close the lid and press down firmly. Press

As the drive is engaged all displays will change to ACTUAL values.

At approximately 150 rev/min the microprocessor checks a signal received from the rotor which typifies its maximum speed and checks the SET speed. Should the SET speed be greater than the maximum allowed for the rotor in use the SET speed will automatically be reduced to the maximum allowed. When in RCF mode the speed check described above is still initiated after the SET RCF value has been converted to the equivalent speed value, if the SET RCF exceeds the maximum allowed it will be reduced to the maximum.