Medilite Microcentrifuges Instruction Manual June 2012

© 2012 Thermo Fisher Scientific Inc. All rights reserved. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye Chemical Co., Ltd. Hypaque is a registered trademark of Amersham Health As. RULON A and Tygon are registered trademarks of Saint-Gobain Performance Plastics. Alconox is a registered trademark of Alconox. Ficoll is a registered trademark of GE Healthcare. Haemo-Sol is a registered trademark of Haemo-Sol. Triton X-100 is a registered trademark of Sigma-Aldrich Co. LLC. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Electron LED GmbH Robert-Bosch-Straße 1 D - 63505 Langenselbold Germany

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc. The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser. Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or errorfree and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document, even if the information in the document is followed properly. This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents.

IM-448-3 printed in June 2012.

For Research Use Only. Not for use in diagnostic procedures.

WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96. It is marked by the following symbol:

Thermo Fisher Scientific has entered into agreements with recycling and disposal companies in all EU Member States for the recycling and disposal of this device. For information on recycling and disposal companies in Germany and on the products of Thermo Fisher Scientific, which fall under the RoHS Directive (Restriction of the use of certain hazardous substances in electrical and electronic equipment), please visit the website www.thermo.com/WEEERoHS

T Table of Contents Chapter 1

Introduction ... 1-1 Characteristics ...1-2 Technical Data ...1-3 Directives, Standards and Guidelines ...1-4 Mains Supply ...1-4 Rotor Selection ...1-5

Chapter 2

Installation ... 2-1 Receive the Unit ...2-2 Prepare the Installation Site ...2-2 Verify Power Configuration ...2-2 Moving the Unit ...2-3

Chapter 3

Operation ... 3-1 Warnings and Cautions ...3-2 Operating Controls ...3-2 Loading ...3-2 Starting a Run ...3-3 Stopping a Run ...3-3

Chapter 4

Maintenance ... 4-1 Care and Cleaning ...4-2

Chemical Compatibility Chart ... A-1 Contact Information ... B-1

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Preface Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions. The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is forbidden to copy or pass on this information without explicit approval. Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty.

Scope of Supply Article number

IM448

Quantity Centrifuge Medilite

1

Rotor

1

CD with instruction manual

1

Check

If any parts are missing, please contact your nearest Thermo Fisher Scientific representative. This symbol refers to general hazards. WARNING means that injuries or material damage or contamination could occur. CAUTION means that material damage could occur.

Intended Use This centrifuge is a laboratory product designed to separate components by generation of relative centrifugal force. The centrifuge is to be used for separating materials of different density or particle size suspended in a liquid. g ----------Maximum sample density at maximum speed: 1.2 cm 3

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P Accident Prevention

Accident Prevention

WARNING If a hazardious situation occurs, turn off the power supply to the centrifuge and leave the area immediately.

WARNING If the centrifuge is used in a manner nor specified in this manual, the protection provided by the centrifuge may be impaired. Prerequisite for the safe operation of the Sorvall Medilite is a work environment in compliance with standards, directives and trade association safety regulations and proper instruction of the user. The safety regulations contain the following basic recommendations: • Maintain a radius of at least 30 cm around the centrifuge. Remaining risk: Inproper use can cause damages, contamincation, and injuries with fatal consequences. • Implementation of special measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it is running. • The mains plug must be freely accessible at all times. Pull out the power supply plug or disconnect the power supply in an emergency.

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P Precautions

Precautions In order to ensure safe operation of the Sorvall Medilite, the following general safety regulations must be followed: • The centrifuge should be operated by trained specialists only. • The centrifuge is to be used for its intended use only. • While handling centrifuge, rotor, and samples you must wear laboratory clothing (e.g. gloves). • Plug the centrifuge only into sockets which have been properly grounded. • Do not move the centrifuge while it is running. • Do not lean on the centrifuge. • Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific. Exceptions to this rule are commercially available glass or plastic centrifuge tubes, provided they have been approved for the speed or the RCF value of the rotor. • Do not use rotors which show any signs of cracks. • Use only with rotors which have been properly installed. Follow the instructions in section “Rotor Installation” on page 4-2. • Use only with rotors which have been loaded properly. • Never overload the rotor. • Never open the door until the rotor has come to a complete stop. • Never use the centrifuge if parts of it are damaged or missing. • Do not touch the electronic components of the centrifuge or alter any electronic or mechanical components. • Please observe the safety instructions.

Please pay particular attention to the following aspects: • Location: well-ventilated environment • Place the centrifuge on a clean, dry surface, to make certain that the suction feet at the bottom grip the surface firmly. • Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts and rotor chamber have to be cleaned carefully. • Always balance the samples.

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Centrifuging hazardous substances: • Do not centrifuge explosive or flammable materials or substances which could react violently with one another. • The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone environment. • Do not centrifuge inflammable substances. Remaining risk: Inproper use can cause damages, contamincation, and injuries with fatal consequences. • Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety precautions. When centrifuging microbiological samples from the Risk Group II (according to the Biosafety Manual" of the World Health Organization (WHO)), aerosol-tight biological seals have to be used. For materials in a higher risk group, extra safety measures have to be taken. • If toxins or pathogenic substances have gotten into the centrifuge or its parts, appropriate disinfection measures have to be taken (see “Disinfection” on page 5-3). Remaining risk: Inproper use can cause damages, contamincation, and injuries with fatal consequences. • Highly corrosive substances which can cause material damage and impair the mechanical stability of the rotor, should only be centrifuged in corresponding protective tubes.

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Introduction Contents

• “Characteristics” on page 1-2 • “Technical Data” on page 1-3 • “Directives, Standards and Guidelines” on page 1-4 • “Mains Supply” on page 1-4 • “Rotor Selection” on page 1-5

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1 Introduction Characteristics

Characteristics The unit consists of: • A baseplate with three rubber suction cup pads for stability. • A brushless AC motor is attached to the cabinet. The motor is thermally protected in accordance with UL and CSA specifications. • A six or twelve place plastic 45° angle rotor. • A 30 minute timer (with hold function). • A viewport in the cover for easy speed verification.

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1 Introduction Technical Data

Technical Data The technical data of the Thermo Scientific Medilite is listed in the following table. Table 1-1. Technical data Thermo Scientific Medilite Feature

Value

Environmental conditions

-Indoor use only -Altitudes of up to 2,000 m above sea level -max. relative humidity 80 % up to 31°C; decreasing linearly up to 50% relative humidity at 40°C

Permissible ambient temperature during operation

+5°C to +40°C

+5°C to +40°C

Permissible ambient temperature during storage and shipping

+2°C to +50°C

+2°C to +50°C

Overvoltage category

II

II

Pollution degree

2

2

Heat dissipation

220-240V

120V

274BTU/h

290BTU/h

IP

20

20

Max running time

30min

30min

Speed nmax

2700rpm

3100rpm

Maximum RCF value at nmax

920x g

1228x g

Maximum kinetic energy

<285Nm

<285Nm

Noise level at maximum speed

< 65dB (A)

< 65dB (A)

241mm 457mmm 292mm 375mm

9.5in 18in 11.5in 14.75in

5.7kg

12.5lbs

Dimensions Hight Hight, cover open Width Depth Weight

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1 Introduction Directives, Standards and Guidelines

Directives, Standards and Guidelines Table 1-2. Directives, Standards and Guidelines Tension / Frequency 220-240V 50Hz/60Hz,

Marked

Produced and inspected according to the following standards and guidelines

CE

EN 61010-1, 2nd Edition

2006/42/EC Machine Directive

EN 61010-2-020, 2nd Edition

2004/108/EC EMC Directive 2006/95/EC Low Voltage Directive 120V 50Hz

UL Std. No. 61010-1 (2nd Edition)

cCSAus:

CAN/CSA-C22.2 No. 61010-1-04 CAN/CSA-C22.2 No. 61010-2-020-09 IEC 61010-2-020 2nd Edition

Mains Supply The following table contains an overview of the electrical connection data for the Thermo Scientific Medilite. This data is to be taken into consideration when selecting the mains connection socket. Table 1-3. Electrical connection data of theThermo Scientific Medilite Cat.

Mains voltage

Frequency

Rated current

Powerconsumption

Equipment fuse

Building fuse

449

Medilite 6-place rotor

220-240 V

50 / 60 Hz

0.6 A

80 W

1.25A, slow blow

16 AT

448

Medilite 6-place rotor

120 V

50 / 60 Hz

1.2 A

85 W

2.5A, slow blowr

15 AT

459

Medilite 12-place rotor

220-240 V

50 / 60 Hz

0.6 A

80 W

1.25A, slow blow

16 AT

458

Medilite 12-place rotor

120 V

50 / 60 Hz

1.2 A

85 W

2.5A, slow blow

15 AT

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1 Introduction Rotor Selection

Rotor Selection The Thermo Scientific Medilite is supplied with a rotor. Two rotors are available to choose from. 6-Place Rotor

47430

12-Place Rotor

47620

Aeroshield 10 mL sealed with cap and o-ring

2087

Tube Size (mm) O.D. x Length Min.

7 x100

O.D.x Length Maximum 6 tubes (in 6 or 12 places)

16x133 or 17x120

O.D.x Length Maximum 12 tubes

16x100

Maximum Volume 6 place

90 mL (6x15 mL)

12 place

130 mL (12x10 mL)

For more information visit our website at: www.thermoscientific.com/centrifuge.

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Installation Contents

• “Receive the Unit ” on page 2-2 • “Prepare the Installation Site” on page 2-2 • “Verify Power Configuration ” on page 2-2 • “Moving the Unit ” on page 2-3

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2 Installation Receive the Unit

Receive the Unit All units are shipped in protective packaging. Inspect the unit upon receipt and immediately file any damage claims with the shipper/carrier.

Prepare the Installation Site • The unit normally resides on a bench top. • Place the centrifuge on a clean, dry surface, to make certain that the suction feet at the bottom grip the surface firmly. Keep the area beneath the unit free of debris and loose materials.. CAUTION The resting surface must be level, to ensure quiet, vibration-free operation. A rigid and stable location is important. An improperly loaded centrifuge may vibrate or move. • A safety zone of at least 30 cm (12”)must be maintained around the centrifuge. WARNING No person or any hazardous materials Should be in the safety zone while the centrifuge operates for the unlikely event of a disruption. • The centrifuge should not be exposed to heat and strong sunlight. WARNING UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories to direct sunlight. • The set-up location must be well-ventilated at all times. • Lift the lever to release the latch. Remove the soft material covering the plastic shields (6 or 12) that are shipped in position inside the rotor. • Press down on the center hub to ensure that the rotor is secured to the shaft. Turn rotor by hand to verify it turns freely. • Inspect the shields for damage. Make sure the flange of the shield is flush with the surface of the rotor.

Verify Power Configuration Verify that the correct power cord and connector is provided for your installation. The unit requires a grounded power supply (3-outlet). If your facility does not have grounded power outlets, arrange for proper grounding.

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2 Installation Moving the Unit

WARNING ELECTRICAL HAZARD! Do not remove the grounding pin from the centrifuge power cord. Do not use the bare wired power cord to attach a power plug that does not have a grounding pin. The power cord provided with the unit is correctly rated for the highest current demand. This power cord should not be interchanged with cords from equipment with lower current demand. Exchange of power cords between equipment may create a fire hazard.

Moving the Unit To move the unit to a new location: WARNING Use caution when moving to avoid any injury

1. Check that the new site meets the criterias described in “Prepare the Installation Site” on page 2-2 before moving the unit. 2. Before moving, unplug the centrifuge and remove all accessories and the rotor. 3. Position a flat object, such as a tongue depressor, near a suction cup at the bottom of the unit. 4. Lift up an edge of the cup, and insert the flat object far enough to break the vacuum suction seal. 5. When all four suction cups are disengaged, lift the unit from the work surface. 6. When the unit is in its new location, ensure that the suction cups adhere correctly to the work surface.

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Operation Contents

• “Warnings and Cautions ” on page 3-2 • “Loading ” on page 3-2 • “Loading ” on page 3-2 • “Starting a Run ” on page 3-3 • “Stopping a Run ” on page 3-3

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3 Operation Warnings and Cautions

Warnings and Cautions WARNING To Avoid Electric Shock: Plug the power cord into a grounded outlet. Never remove the grounding prong from the power plug, or use any adapter which does not complete the grounding circuit. Always unplug the power cord before attempting to clean or service the centrifuge. CAUTION Do not exceed maximum rated speed for each rotor/ accessory combination. Maximum speeds can be found in “Technical Data” on page 1-3. All rotors and accessories are stamped with their cat. no.for easy identification. Do not spin any fluids which have a specific gravity greater than 1.5. Do not operate the unit if the shields or rotor show signs of damage. If deterioration is visible, replace the affected parts immediately. Do not open the lid while the unit is spinning. Ensure that loads are properly balanced around the rotor to minimize vibration. All Thermo Fisher Scientific accessories are stamped with their weight for easy balancing. Chemical damage can appear as crazing, frosting, peeling or similar deterioration of the inner cavity or exterior surface of the shields or rotor. Mechanical damage can appear as cracks, scratches or gouges on the shield or rotor surfaces.

Operating Controls

Lid Opener

Timer

Loading 1. Unlatch and open the cover. 2. Check that the rotor turns freely and that there are no loose objects in the chamber.

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3 Operation Starting a Run

3. Load the rotor symmetrically with 2, 3, 4, or 6 tubes in the 6-place rotor (but not 1 or 5) or 2, 3, 4, 6, 8, 9, or 12 in the 12-place rotor (but not 1, 5, 7 or 11). The tubes must be of equal size and with contents of equal (within 1 gram) weight. 4. Close the cover securely, ensuring that the latch is engaged.

Starting a Run 1. Turn the timer knob to the desired run duration. For runs of under 5 minutes, turn the timer past the five minute setting and then back to the desired time). For runs in the HOLD mode (infinite duration), turn the knob speed counter-clockwise to the ∞setting. The motor will take several minutes to reach top speed.. Note Periods of slight vibration during acceleration are normal. If excessive vibration occurs, turn the timer knob to zero (0). When the rotor has come to a stop, open the cover and check that the load is balanced. If the rotor is properly balanced and vibration persists, ensure that the suction cup feet are clean and adhering to the surface on which the unit was placed.

Stopping a Run 1. To stop a run, turn the timer knob to zero or allow the time to expire. 2. Wait until the rotor has come to a complete stop, then unlatch and open the cover.

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Maintenance Contents

• “Care and Cleaning ” on page 4-2

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