Nunc CryoFlex Tubing Instructions for Use Ver 4.1

Thermo Scientific Nunc CryoFlex Tubing – Instructions for Use

1

Cut a length of Nunc™ CryoFlex™ Tubing sufficient to extend 2 cm beyond the ends of the Nunc CryoTube™ Vial.

3

2

Place sample in CryoTube; tighten screw cap normally. Center the CryoTube in the CryoFlex.

4

Heat tubing gently while rotating, using a heat gun to shrink the tubing around the CryoTube.

Reheat end of the tubing and squeeze or crimp for top and bottom seal. Trim any excess tubing and, if desired, melt the crimped ends.

6

5

Remove sample by cutting

around the tubing at the base of the cap and pull vial from tubing.

To use as a “cane” for lowering CryoTube into liquid nitrogen: a) Heat and crimp one end of a full 50 cm length of CryoFlex. b) Insert CryoTube Vials successively. c) Heat and crimp between individual CryoTubes or at the top end of the CryoFlex ”cane” as desired.