Operating Manual
40 Pages
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KF 2 ICS Transmitted Light Microscope
Operating Manual
Carl Zeiss
KF 2 ICS
Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. The specifications are subject to change; the manual is not covered by an update service. ©
Unless expressly authorized, forwarding and duplication of this document, and the utilization and communication of its contents are not permitted. Violations will entail an obligation to pay compensation. All rights reserved in the event of granting of patents or registration of a utility model.
Issued by:
Carl Zeiss Microscopy D-07740 Jena Phone: ++49-36 41 64-16 16 Telefax: ++49-36 41 64-31 44 E-mail: [email protected] Internet: www.zeiss.de/micro
Number of this manual: B 40-028 e Date of issue: 10/97
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KF 2 ICS
Carl Zeiss
CONTENTS Page INTRODUCTION ... I Title page ... I Copyright ... II Contents ...III Notes on instrument safety ... IV Notes on warranty ...V Overall view... VI 1 1.1 1.2 1.3 1.4
DESCRIPTION ... 1-3 Terms, intended use ... 1-3 Instrument description ... 1-3 Modules ... 1-4 Technical data ... 1-6
2 2.1
START-UP... 2-3 Installation of the instrument ... 2-3
3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9
OPERATION ... 3-3 Objective selection... 3-3 Visual overall magnification... 3-4 Eyepiece reticles... 3-4 Setting the microscope in brightfield ... 3-6 Setting the microscope for phase contrast... 3-8 Setting the microscope in darkfield ... 3-10 Photomicrography ... 3-11 Videomicroscopy ... 3-12 illumination via mirror and condensor 0.9 AS ... 3-12
4 4.1 4.2
CARE, MAINTENANCE AND TROUBLESHOOTING ... 4-3 Care and maintenance of the instrument ... 4-3 Troubleshooting and requesting service... 4-4 ANNEX ... A-1 List of abbreviations... A-3 Physical and technical units ... A-4 EC conformity declaration... A-5 Certification in accordance with DIN EN ISO 9001 / DIN EN 46001 ... A-7
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KF 2 ICS
Notes on instrument safety The KF 2 ICS microscope, including its original accessories, may only be used for the microscope applications described in this manual.
The manufacturer cannot assume any liability for any other applications, possibly also involving individual modules or single parts. This also applies to all service or repair work which is not carried out by authorized service personnel. Furthermore, all guarantee/warranty claims also expire for all those parts which were not directly affected by the repair.
Particular attention must be paid to the following warning notes: The KF 2 ICS microscope was designed, produced and tested in compliance with DIN EN 61010-1 (IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments, and meets the requirements of appendix I of directive 73/23/EC and the relevant CSA and UL directives. The instrument meets the requirements of the EC directive 89/336/EC and the EMC legislation of November 9th 1992. This operation manual includes information and warnings which must be observed by the user.
The KF 2 ICS microscope is categorized as Protection Class I and has been allocated the protection degree IP 20. The power plug must be inserted in a properly installed socket featuring a grounding contact. The grounding effect must not be made ineffective by an extension cable which does not have a protective ground wire.
Before switching on the instrument, check whether it is suitable for the line voltage present. The voltage is indicated on the underside of the microscope stand. Incorrect voltage settings can destroy the instrument.
If it is established that the protection measures are no longer effective, the instrument must be switched off and safeguarded against inadvertent operation. For the repair of the KF 2 ICS microscope, please contact the Carl Zeiss Microscopy Service in Germany (see page 4-6) or your local Carl Zeiss agency.
Dust and dirt can impair the performance of the KF 2 ICS microscope. Therefore, protect the instrument against these influences as far as possible. If the microscope will not be used for longer periods of time (more than 6 hours), it must be protected using the dust cover.
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The instrument must be operated by trained personnel only, who must be aware of the possible danger involved with microscopy and the relevant application.
The KF 2 ICS microscope is an optical precision instrument which can be impaired in its performance or damaged when handled improperly.
All repairs inside the instrument may only be performed by the Carl Zeiss Microscopy Service in Germany (see page 4-6) or your local Carl Zeiss agency.
Notes on warranty: The manufacturer guarantees that the instrument has no material and production defects when delivered. You must inform us of any defects immediately and we must do anything to minimize the damage. If the manufacturer is informed of such a defect, he is obliged to remove it; it is his decision whether he does this by repairing the instrument or by delivering an instrument free of any defect. No guarantee is provided for defects caused by natural wear (wearing parts in particular).
The instrument manufacturer is not liable for damage caused by faulty operation, negligence or any other meddling with the instrument, or the use of accessories from other manufacturers. This forfeits all the claims against warranty.
With the exception of the work specified in this manual, no maintenance or repair of the KF 2 ICS may be performed. Repairs may only be performed by Carl Zeiss Microscopy Service staff or specially authorized personnel. Should any defect occur with the instrument, please contact the Carl Zeiss Microscopy Service in Germany (see page 4-6) or your local Carl Zeiss agency..
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Carl Zeiss
KF 2 ICS
Overall view of the KF 2 ICS microscope
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Carl Zeiss
DESCRIPTION Contents 1 1.1 1.2 1.3 1.4
DESCRIPTION ...1-3 Terms, intended use ...1-3 Instrument description ...1-3 Modules ...1-4 Technical data ...1-6
List of illustrations Fig. 1-1
KF 2 ICS microscope equipment...1-4
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DESCRIPTION
1.1
Terms, intended use
Manufacturer's name: KF 2 ICS microscope The KF 2 ICS microscope is a universal microscope for the visualization of fine structures and forms in biology and medicine. These are typical application fields of this microscope: -
Schools, universities
-
Medicine (for diagnosis)
-
Quality inspection
1.2
Instrument Description
The KF 2 ICS microscope is a small, compact instrument which permits high-performance transmittedlight microscopy at little effort. Major features: -
Sturdy, compact stand
-
Coaxial coarse and fine drive
-
Microscope stage with optional stand configuration: Fixed stage with spring clamps or mechanical stage 75 25 with specimen holder L or mechanical stage 75 25 with specimen holder R
-
Condenser 0.9 AS with aperture diaphragm for low-power observation
-
4-position nosepiece with W 0.8 thread
-
ICS objectives
-
Binocular tube 45°/20 ICS
-
Built-in illuminator with 6V 10W halogen lamp
-
Power unit with integrated, stabilized power supply
-
12 V battery operation on request
-
Illumination using external light source possible via mirror
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KF 2 ICS
1.3
Modules
Fig. 1-1
KF 2 ICS microscope equipment
1-4
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Carl Zeiss
KF 2 ICS microscope equipment
Cat. No.
KF 2 ICS microscope stand with 4-position W 0.8 nosepiece, condenser 0.9 AS with aperture diaphragm and additional lens for low-power observation, built-in illuminator, stabilized power supply, power cable with safety plug or American flat plug, 2 halogen lamps 6V/10W and instrument protection cover. 1.1
KF 2 ICS stand equipment with fixed stage and 2 spring clamps (230 V) KF 2 ICS stand equipment with fixed stage and 2 spring clamps (115 V)
450719-9901-000
KF 2 ICS stand equipment with mechanical stage 75 25 L and specimen holder with spring clip R (230 V) KF 2 ICS stand equipment with mechanical stage 75 25 L and specimen holder with spring clip R (115 V)
450713-9901-000
2
Binocular tube 45°/20 ICS
452928-0000-000
3.1
Eyepiece PL 10/18 Br. foc.
444132-9901-000
3.2
Eyepiece PL 10/18 Br.
444131-9901-000
4.1
CP-"Achromat" objective 5/0.12
440920-0000-000
4.2
CP-"Achromat" objective 10/0.25
440930-0000-000
4.3
CP-"Achromat" objective 40/0.65
440950-0000-000
4.4
CP-"Achromat" objective 100/1.25 Oil
440980-0000-000
4.5
CP-"Achromat" objective 40/0.65 Ph 2 (alternative to 440950) Ph 2 equipment Diopter d = 30 mm
440951-0000-000 465214-0000-000 444020-0000-000
5
Instrument protection cover
1.2
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450720-9901-000
450714-9901-000
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KF 2 ICS
Technical Data Dimensions
Dimensions (width depth height) ...185 185 400 mm (2) Weight Stand equipment... approx. 3 kg Binocular tube 45°/20 ICS... approx. 1 kg KF 2 ICS microscope, complete ... approx. 5 kg (3) Ambient conditions Storage and transport (in packaging): Permissible ambient temperature ... -40 to +70° C Permissible relative humidity (without condensation) ...max. 100 % Operation: Permissible ambient temperature ... +10 to +40° C Permissible relative humidity (without condensation) ...max. 90 % (4) Operating data Category of use... closed rooms Protection class... SK 1 Protection degree ... IP 20 Electrical safety...in compliance with DIN EN 61010 (IEC 1010-1) including CSA and UL directives Pollution degree ... 2 Excess voltage category ...II Radio interference suppression... in accordance with EN 55011, Class B Line voltage... 100 to 127 VAC (10%) or 220 to 240 VAC (10%) Line frequency...50/60 Hz Label of voltage range ... on instrument underside max. power consumption ... 35 VA battery supply voltage ...12 V DC (5) Light source Built-in illuminator system with halogen lamp... 6 V, 10W Lamp voltage (variable) ...0 to max. 6 V (6) Opto-mechanical data Stand with objective focusing ...coaxial coarse (fine drive, lift: 15 mm coarse drive: 1 rotation = 4 mm, fine drive: step-down ratio 1:10 Objective change... manually via 4-position nosepiece W 0.8" Largest possible eyepiece field... 20 mm Largest possible object field ... 5 mm Binocular tube ... interpupillary distance can be set between 55 and 75 mm
1-6
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START-UP Contents 2 2.1
START-UP...2-3 Installation of the instrument ...2-3
List of illustrations Fig. 2-1 Fig. 2-2 Fig. 2-3 Fig. 2-4 Fig. 2-5 Fig. 2-6
Attachment of binocular tube ...2-3 Positions of binocular tube...2-3 Screwing in objectives...2-4 Attachment of eyepieces ...2-4 Switch on line operation mode ...2-5 Switch on line/battery operation mode...2-6
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START-UP
2.1
Installation of the instrument
The KF 2 ICS microscope, including accessories, is delivered in standard packaging. We would recommend you to keep the packaging material so that the instrument can be stored for a longer period of time or returned to the manufacturer. (1)
Preparations
Remove the microscope from the transport case and place it on the worktable. (2)
Attachment of binocular tube 45°/20 ICS
Loosen hexagonal screw (2-1/2) using the SW 3 ball-headed screwdriver. Slightly incline the tube (2-1/1) and insert it into the stand via its dovetail. Turn the tube in the required observation position and tighten the hexagonal screw.
Fig. 2-1
Attachment of binocular tube
Fig. 2-2
Positions of the binocular tube
The eyepiece distance is matched to the
individual interpupillary distance by swinging the eyepiece tubes symmetrically towards one another. A higher (2-2/A) or lower (2-2/B) viewing height
is achieved by swiveling the tube.
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(3)
Screwing in objectives
Remove dust caps (2-2/2) according to the number of objectives and screw objectives (2-2/3) in nosepiece clockwise one by one, starting with the lowest magnification. Nosepiece eyes which are not required should be closed with dust caps.
Fig. 2-3
Screwing in objectives
(4)
Inserting the eyepieces
Remove both protection caps (2-4/1) from the
binocular tube (2-4/2). Insert the fixed eyepiece PL 10x/18 Br. (2-4/3) in
the right tube and the focusing eyepiece PL 10x/18 Br. foc. (2-4/4) in the left tube. The focusing eyepiece is used to compensate for myopia of both eyes.
Fig. 2-4
2-4
Inserting the eyepieces
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Switching on the line and line/battery modes
The illumination system of the KF 2 ICS has been designed for operation using a power unit, but is also available for line/battery operation on request. Subsequent conversion from line operation to line/battery operation is not possible.
View the type label (4-2/2) to check whether the built-in lamp supply complies with the present line voltage. Should the line voltage not lie within the range of the built-in lamp supply, please get in touch with the nearest Carl Zeiss agency.
a)
Switch on line operation
Connect instrument to the power plug via the
power cable. Set the required brightness via the control
(2-5/2). Depending on requirements, place attenuation
filter or conversion filter on support (2-5/3). Swing required objective (2-5/1) in the beam
path.
Fig. 2-5
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Switching on the line operation
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Carl Zeiss
b)
KF 2 ICS
Switch on the line/battery operation
Fig. 2-5
Switching on the line/battery operation
Line operation Connect KF 2 ICS to line plug via line cable. Set switch (2-6/4) to "Mains ON". Set the required brightness using the "Dimmer Mains" control (2-6/2). Battery operation: Connect plug (2-6/10) of the "Control panel for battery" to socket (2-6/5). Set switch (2-6/4) on the Standard KF 2 ICS in position "Battery ON". Set switch (2-6/7) on the "Control panel for battery" in position "Battery off". Connect "Control panel for battery" to car battery (12 V DC) via connection clamps (2-6/6).
Note the correct polarity of the connection clamps! Set switch (2-6/7) on the "Control panel for battery" in position "Battery on". The green LED (2-6/8) lights up. Set the required brightness using the "Dimmer Battery" control (2-6/9). Depending on requirements, place attenuation filter or conversion filter on support (2-6/3). Swing required objective (2-6/1) in the beam path.
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OPERATION Contents 3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9
OPERATION ...3-3 Objective selection...3-3 Visual overall magnification...3-4 Eyepiece reticles...3-4 Setting the microscope in brightfield ...3-6 Setting the microscope for phase contrast ...3-8 Setting the microscope in darkfield ... 3-10 Photomicrography ... 3-11 Videomicroscopy ... 3-12 Illumination via mirror and condenser 0.9 AS... 3-12
List of illustrations Fig. 3-1 Fig. 3-2 Fig. 3-3 Fig. 3-4 Fig. 3-5 Fig. 3-6 Fig. 3-7 Fig. 3-8 Fig. 3-9
Inserting an eyepiece reticle ...3-4 Setting the condenser lever...3-6 Setting the microscope in brightfield ...3-7 Setting the microscope for phase contrast...3-8 Setting the Ph rings via diopter or auxiliary microscope ...3-8 Illustration of the phase stop setting ... 3-9 Setting the microscope in darkfield ... 3-10 Attaching a SLR camera (CONTAX 167 MT)... 3-11 Illumination via mirror and condenser 0.9 AS... 3-12
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