Operating Manual
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Standard 25 ICS Transmitted Light Microscope
Operating Manual
Standard 25 ICS
Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. The specifications are subject to change; the manual is not covered by an update service. ©
Unless expressly authorized, forwarding and duplication of this document, and the utilization and communication of its contents are not permitted. Violations will entail an obligation to pay compensation. All rights reserved in the event of granting of patents or registration of a utility model.
Issued by:
Carl Zeiss Mikroskopie D-07740 Jena Telephone: (**49) 03641 / 64-1616 Telefax: (**49) 03641 / 64-3144 Internet: [email protected] http://www.zeiss.de
Number of this manual: B 40-029 e Date of issue: 01/98
II
B 40-029 e 01/98
Standard 25 ICS
CONTENTS
Page INTRODUCTION... I Title page ... I Copyright... II Contents ...III Notes on instrument safety ... IV Warranty notes ... VI Overall view ... VII
1 1.1 1.2 1.3 1.4 1.5 1.6 1.7
DESCRIPTION... 1-3 Name and intended application ...1-3 Instrument description and main features... 1-3 Microscope configurations and modules ... 1-4 Objectives ... 1-6 Eyepieces ... 1-7 Stage micrometers and eyepiece reticles ... 1-8 Technical Data... 1-10
2 2.1 2.2 2.3 2. 2.4.1 2.4.2 2.5 2.6 2.7 2.7.1 2.7.2 2.8
START-UP ... 2-3 Unpacking the instrument ... 2-3 Attachment of binocular tube 45°/20 ICS or binocular phototube 35°/20 ICS... 2-3 Screwing-in of objectives ... 2-3 Insertion of eyepieces ... 2-4 Insertion of eyepiece reticle... 2-4 Compensation of ametropia when eyepiece reticles are used ... 2-5 Setting of interpupillary distance and viewing height ... 2-5 Attachment of specimen holder to mechanical stage 75x30 R ... 2-6 Attachment of condensers... 2-7 Attach condenser 0.9 Z ... 2-7 Attach condenser II Z 0.9 Ph1, 2, 3 and D (0.6/0.9) ... 2-7 Connecting the instrument to the line... 2-8
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Standard 25 ICS
3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.7.1 3.7.2 3.8 3.9
OPERATION... 3-3 Switch on the instrument ... 3-3 Setting of transmitted-light brightfield for KÖHLER illumination... 3-4 Setting of transmitted-light phase contrast ... 3-6 Setting of transmitted-light darkfield ... 3-8 Setting of transmitted light polarization contrast... 3-9 Measurement of lengths ... 3-10 Attachment of photomicrography equipment ... 3-11 Attachment of SLR camera, e.g. CONTAX 167 MT ... 3-11 Attachment of MC 80 DX microscope camera (35 mm film cassette)... 3-13 Attachment of adapters for video cameras... 3-14 Insertion of 8x drawing eyepiece ... 3-16
4 4.1 4.2 4.2.1 4.2.2 4.2.3 4.3 4.4
CARE, MAINTENANCE AND TROUBLESHOOTING... 4-3 Care and maintenance of the instrument... 4-3 Troubleshooting... 4-4 Changing the fuses ... 4-4 Changing the 6 V 20 W halogen lamp... 4-5 Troubleshooting table ... 4-6 Table of spares, wearing parts and tools ... 4-10 Requesting service... 4-11
Annex...A-1 List of abbreviations ...A-3 Physical and technical units...A-4 Certification in accordance with DIN EN ISO 9001 / DIN EN 46001...A-5 EC conformity declaration ...A-7
IV
B 40-029 e 01/98
Standard 25 ICS
Notes on instrument safety The Standard 25 ICS microscope was designed, produced and tested in compliance with DIN 61010-1 (IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments, and meets the requirements of appendix I of directive 73/23/EC and the relevant CSA and UL directives. The microscope meets the requirements of the EC directive 89/336/EC and the EMC legislation of November 9, 1992. This operation manual includes information and warnings which must be observed by the user.
The following warning and information symbols are used in this manual:
NOTE This symbol (hand) is a warning which you must observe under all circumstances.
CAUTION This symbol (warning triangle) is a warning which indicates a hazard to the operation of the instrument.
CAUTION Disconnect the instrument from the line!
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V
Standard 25 ICS
The Standard 25 ICS microscope, including its original accessories, may only be used for the microscope techniques described in this manual. Particular attention must be paid to the following warning notes:
The Standard 25 ICS microscope is categorized as Protection Class I and has been allocated the protection degree IP 20. The power plug must be inserted in a properly installed socket featuring a grounding contact. The grounding effect must not be made ineffective by an extension cable which does not have a protective ground wire.
Before switching on the instrument, check whether it is suitable for the line voltage present. Admissible voltage: 100...240 V AC, 50...60 Hz (depending on the model). Always disconnect the instrument from the line before opening the instrument, before changing the voltage and before changing the fuses.
When changing the instrument fuses, make sure to use only those of the rated power required and the type indicated. The use of makeshift fuses and the short-circuiting of the fuse holders are not permitted.
If it is established that the protection measures are no longer effective, the instrument must be switched off and safeguarded against inadvertent operation.
Dust and dirt can impair the performance of the instrument. Therefore, protect the instrument against these influences as far as possible. If the microscope will not be used for longer periods of time, it must be protected using the dust cover.
The instrument must be operated by trained personnel only who must be aware of the possible danger involved with microscopy and the relevant application.
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Standard 25 ICS
The Standard 25 ICS microscope is an optical precision instrument which can be impaired in its performance or damaged when handled improperly.
Notes on warranty:
The manufacturer guarantees that the instrument has no material and production defects when delivered. You must inform us of any defects immediately and we must do anything to minimize the damage. If the manufacturer is informed of such a defect, he is obliged to remove it; it is his decision whether he does this by repairing the instrument or by delivering an instrument free of any defect. No guarantee is provided for defects caused by natural wear (wearing parts in particular).
The instrument manufacturer is not liable for damage caused by faulty operation, negligence or any other meddling with the instrument, or the use of accessories from other manufacturers. This forfeits all the claims against warranty.
With the exception of the work specified in this manual, no maintenance or repair of the Standard 25 ICS may be performed. Repairs may only be performed by Zeiss service staff or specially authorized personnel. Should any defect occur with the instrument, please get in touch with your local Zeiss agency first.
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VII
Standard 25 ICS
Overall view of the Standard 25 ICS microscope with polarization equipment
VIII
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Standard 25 ICS
DESCRIPTION Contents 1 1.1 1.2 1.3 1.4 1.5 1.6 1.7
DESCRIPTION ...1-3 Name and intended application...1-3 Instrument description and main features...1-3 Microscope configurations and modules...1-4 Objectives...1-6 Eyepieces...1-8 Stage micrometers and eyepiece reticles...1-8 Technical Data ... 1-10
List of illustrations Fig. 1-1 Fig. 1-2
Microscope modules...1-2 Standard 25 ICS microscope configurations...1-4
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1-1
Standard 25 ICS
Fig. 1-1 1 2 3 4
Microscope modules
Eyepieces Binocular tube Stand Coaxial coarse and fine drive
1-2
5 6 7 8
Condenser carrier with condenser Mechanical stage with specimen holder Objective Objective nosepiece
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Standard 25 ICS
1
DESCRIPTION
1.1
Name and intended application
Manufacturer's name: Standard 25 ICS microscope The Standard 25 ICS microscope is a universal microscope for the visualization of fine structures and forms in biology and medicine. These are typical application fields of the Standard 25 ICS: laboratory microscopy doctor's offices training (schools and universities).
1.2
Instrument description and main features
Thanks to its pyramid design, the Standard 25 ICS is a compact transmitted-light microscope. In addition to high-resolution ICS objectives and the major brightfield, darkfield, phase contrast and polarization contrast techniques, an optional camera port for photo and video documentation is also available to the user. Major instrument features: Sturdy and convenient stand in the pyramid design. User-friendly coaxial coarse and fine drive Mechanical stage 75x30 R with ceramic-coated stage surface and specimen holder Space-saving and continuously adjustable, integrated illuminator with long-life 6V 20W halogen lamp. Swing-in 0.9 Z condenser for brightfield, and Ph condenser II Z 0.9 for brightfield and phase contrast
Ph1, 2 and 3. Ball-bearing, 5-position nosepiece with W 0.8" thread. ICS objectives in the price/performance categories CP-Achromat, A-Plan and Achroplan. Binocular tube 45°/20 ICS with a viewing height of approx. 440 mm, and binocular phototube 35°/20
with sliding prism 100 obs /100 doc. 10x eyepieces for the field numbers 20 or 18, suitable for spectacle wearers, fixed and adjustable.
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1-3
Standard 25 ICS
1.3
Microscope configurations and modules
Fig. 1-2
Standard 25 ICS microscope configurations with polarization equipment
1-4
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Standard 25 ICS
Cat. No. Configurations Standard 25 ICS binocular microscope for transmitted-light brightfield and phase contrast using CP Achromat objectives 10x, 40x Ph2 and 100x "Standard 25 ICS" binocular microscope for transmitted-light brightfield using objectives "A-Plan" 10x, 40x and 100x "Standard 25 ICS" binocular microscope for transmitted-light brightfield and phase contrast using objectives "A-Plan" 10x, 40x Ph 2 and 100x "Standard 25 ICS" binocular microscope for transmitted-light brightfield and phase contrast using objectives "A-Plan" 10x, 20x Ph 2 and 40x Ph 2 "Standard 25 ICS" binocular microscope for transmitted-light brightfield and phase contrast using objectives "A-Plan" 10x Ph 1, 40x Ph 2 and 100x Ph 3 "Standard 25 ICS" binocular microscope for transmitted-light brightfield using objectives "A-Plan" 10x, 40x and 100x and phototube "Standard 25 ICS" binocular microscope for transmitted-light brightfield and phase contrast using objectives "A-Plan" 10x, 40x Ph 2 and 100x and phototube "Standard 25 ICS" binocular microscope for transmitted-light brightfield and phase contrast using objectives "A-Plan" 10x Ph 1, 40x Ph 2 and 100x Ph 3 and phototube Modules 1.1
"Standard 25 ICS" microscope stand with mechanical stage 75x30 R, 5-position nosepiece W 0.8, including integrated illuminator stabilized power supply 220 ... 240 V/50 ... 60 Hz / 45 VA
1.1.1
Line cable with European plug
1.2
"Standard 25 ICS" microscope stand with mechanical stage 75x30 R, 5-position nosepiece W 0,8, including integrated illuminator stabilized power supply 100 ... 127 V / 50 ... 60 Hz / 45 VA
490841 9804 490841 9904 490845 9804 490845 9904 490846 9804 490846 9904 490847 9804 490847 9904 490848 9804 490848 9904 490865 9804 490865 9904 490866 9804 490866 9904 490868 9804 490868 9904
450815 9902
450816 9902
1.2.1
Line cable with American flat plug
1.3
Long-life 6 V 20 W halogen lamp (spare lamp)
380079 9690
1.4
Conversion filter CB 3; d=32x2 mm
467852
1.5
Conversion filter CB 12, d=32x2 mm
467850 9901
1.6
Interference wide-band filter, green, d=32x4 mm
467803
2.1
Binocular tube 45°/20 ICS
452928
2.2
Binocular phototube 35°/20 ICS (100% obs / 50% obs : 50% doc)
452929
3.1
Swing-in 0.9 Z condenser
445211
3.2
Phase stop carrier Ph 2 for 445211
470864
3.3
Phase contrast condenser II Z 0.9 Ph 1, 2, 3 and D (0.6/0.9)
445210
not shown
Dry darkfield condenser 0.7 - 0.85 and Z condenser holder
465506, 445215
not shown
Dry darkfield condenser 0.8 - 0.95 and Z condenser holder
465505, 445215
not shown
Ultra-condenser 1.2 - 1.4 and Z condenser holder
465500, 445215
3.4
Diopter d=30 mm
444020
4.1
Dust cover K
459300
4.2
Dust cover G (if binocular phototube is used)
459306
All the above microscope configurations with catalogue numbers ending in 9804 feature the line voltage 230 V, and those ending in 9904 have a line voltage of 115 V.
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Standard 25 ICS
1.4
Objectives
The objectives are the optical centerpiece of the microscope. The following is an example of how objectives can be labelled: CP ACHROMAT 10x/0.25 /where 10x
=
0.25
=
objective magnification, with a defined color ring on the objective being allocated to each magnification step (Zeiss color code) numerical aperture
= :
infinite tube length can be used with cover slip thickness D = 0 or 0.17
=
can be used with cover slip thickness D = 0.17
= =
oil immersion objective phase contrast objective with a green color ring and phase stop Ph 2
or 0.17 and Oil Ph 2
The objective magnification multiplied by the eyepiece magnification (minimum 10x) results in the visual overall magnification, e.g. 10 x 10 = 100x. The numerical aperture x 1000, e.g. 0.25x1000 = 250x, is the highest useful magnification, i.e. no further details are resolved above that limit. The objective labeling indicates that these objectives may only be used with microscopes featuring an infinite tube length and not with instruments the objectives of which are marked with "160" as their mechanical tube length. The exact observance of the cover slip thickness of 0.17 mm is all the more necessary the higher the numeric aperture of the objective. Therefore, so-called "Corr" objectives can be set for different cover slip thicknesses via a correction ring. For this, a specimen area is searched, and the position of the correction ring where optimum focus and image contrast are obtained is determined (refocusing is always required). Immersion objectives are always insensitive to differences in cover slip thickness. When immersion objectives are used, the air between the cover slip and the objective is replaced with a liquid, which is immersion oil in most cases. The plastic oiler containing 20 ml of 581 N immersion oil is particularly suitable for this purpose. Due to their short working distance, objectives 25x and higher feature resilient mounts (specimen protection). To prevent oil contamination of the specimen when the nosepiece is turned, the resilient mounts of the immersion objectives can be locked in their lifted position by turning them to the right (do not forget to unlock them again!).
1-6
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Standard 25 ICS
The following objectives are available for the Standard 25 ICS microscope: Microscopy Technique
Transm.-light brightfield
Phase contrast
Transm-light brightfield
Phase contrast
Transm.-light brightfield
Phase contrast
Transm.-light brightfield
Phase contrast
Transm.-light brightfield
B 40-029 e 01/98
Objective
Magnification/Num. Aperture
Free working distance in mm
Cover slip thickness D in mm
Cat.No.
CP-Achromat CP-Achromat CP-Achromat CP-Achromat CP-Achromat
5x/0.12 10x/0.25 40x/0.65 100x/1.25 Oil
11.2 5.1 0.3 0.07
0.17 0.17
440920 440930 440950 440980
CP-Achromat CP-Achromat CP-Achromat CP-Achromat
10x/0.25 Ph 1 40x/0.65 Ph 2 100x/1.25 Oil Ph 2 100x/1.25 Oil Ph 3
5.1 0.3 0.07 0.07
0.17 0.17 0.17
440931 440951 000000 1007 159 440981
A-Plan A-Plan A-Plan A-Plan A-Plan A-Plan
5x/0.12 10x/0.25 20x/0.45 40x/0.65 100x/1.25 Oil
9.9 4.4 0.53 0.43 0.22
0.17 0.17 0.17
441020 441030 441040 441050 441080
A-Plan A-Plan A-Plan A-Plan
10x/0.25 Ph 1 20x/0.45 Ph 2 40x/0.65 Ph 2 100x/1.25 Oil Ph 3
4.4 0.53 0.43 0.22
0.17 0.17 0.17
441031 441041 441051 441081
Achrostigmat Achrostigmat Achrostigmat
20x/0.45 40x/0.85 Oil
1.6 0.28
0.17 0.17
440140 440250
Achrostigmat LD-Achrostigmat LD-Achrostigmat
20x/0.45 Ph 2 20x/0.30 Ph 1 32x/0.40 Ph 1
1.6 1.0...2.2 1.5...2.2
0.17 0...2.0 0.5...1.5
440141 440147 440149
Achroplan Achroplan Achroplan Achroplan Achroplan Achroplan Achroplan Achroplan
4x/0.10 10x/0.25 20x/0.45 40x/0.65 50x/0.90 Oil 63x/0.80 63x/0.95
11.1 4.8 2.07 0.59 0.29 0.29 0.15
440020 440030 440040 440050 440057 440060 440068
Achroplan Achroplan
100x/1.25 Oil 100x/1.25 Oil Iris
0.19 0.19
0.17 0.17 0.17 0.17 0 (no cover slip) 0.17 0.17
Achroplan Achroplan Achroplan Achroplan
10x/0.25 Ph 1 20x/0.45 Ph 2 40x/0.65 Ph 2 100x/1.25 Oil Ph 3
4.8 2.07 0.59 0.19
0.17 0.17 0.17
440031 440041 440051 440081
Plan-Neofluar Plan-Neofluar
2.5x/0.075
9.3
-
440310
440080 440086
1-7
Standard 25 ICS
1.5
Eyepieces
The following eyepieces are offered for the Standard 25 ICS:
Eyepiece
Image Angle
Cat. No.
Eyepiece PL 10x/18 Br.
39°
444131 9901
Eyepiece PL 10x/18 Br. foc.
39°
444132 9901
Eyepiece E-PL 10x/20 Br.
43°
444231 9901
Eyepiece E-PL 10x/20 Br. foc.
43°
444232 9902
Eyepiece PL 16x/16 Br.
54°
444053
Eyepiece PL 16x/16 Br. foc.
54°
444054
If required, eyecups for the eyepieces can be ordered under the Cat. No. 444801. 1.6
Stage micrometers and eyepiece reticles
Measuring and counting using the microscope requires stage micrometers and eyepiece reticles, a small selection of which is listed below: Illustration
Description, Technical Data
Cat. No.
Stage micrometer, positive 5 + 100/100 y D 474026 = 0.17 mm gradation on the + y-axis: 5 mm in 5 intervals; gradation on the - y-axis: 1 mm in 100/100 mm = 10 m, accuracy 1m
Crossline micrometer disk 14:140 / d =26 mm gradation length=14 mm increments = 0.1mm gradation tolerance 0.001 mm
1-8
454060
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Standard 25 ICS
Crossline disk / d = 26 mm
474064
Crossline micrometer disk 10:100 / d =26 mm gradation length=10 mm increments = 0.1mm gradation tolerance 0.001 mm
474066 9901
Net micrometer 12.5x12.5/5;10 / d = 26 mm 474068 area 12.5x12.5 mm, divided in fields of 5x5 or 10x10.
Photo reticle MC 2.5x / d = 26 mm 454075 for 35 mm photography with an additional magnification of 2.5x or for large-format photography with a 10x additional magnification.
If an eyepiece reticle is used, the binocular tube or the phototube must be equipped with two foc. eyepieces containing an adjustable eyelens, into one of which the eyepiece reticle is mounted.
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Standard 25 ICS
1.7 (1)
Technical Data Dimensions (width x depth x height)
Stand with binocular tube... approx. 200 x 350 x 450 mm Stand with phototube ... approx. 200 x 375 x 460 mm Overall height including T2 adapter and CONTAX 167 MT camera housing... approx. 570 mm (2)
Weight
Standard 25 ICS with binocular tube... approx. 6.7 kg (3)
Ambient conditions
Storage and transport (in packaging): Permissible ambient temperature ... -40 to + 70° C Permissible relative humidity (without condensation) ...max. 100 % Operation: Permissible ambient temperature ... +10 to + 35° C Permissible relative humidity (without condensation) ...max. 85 % Altitude... max. 2000 m Atmospheric pressure ... 800 hPa to 1060 hPa (4)
Operating data
Operation... closed rooms Protection class...I Enclosure protection... IP 20 Electrical safety...in compliance with DIN EN 61010 (IEC 1010-1) ...including CSA and UL directives Pollution degree ... 2 Overvoltage category...II Radio interference suppression... in accordance with EN 55011, Class B Line voltage... 100 to 127 V AC (10%) or 220 to 240 V AC (10%) Line frequency... 50 to 60 Hz Label of voltage range ... see instrument rear Power consumption...max. 45 VA Output voltage ...stabilized, adjustable from 1.5 to 6 V
1-10
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Standard 25 ICS
(5)
Fuses according IEC 127:
for 230 V...T 0.2 A; 250 V; 5 x 20 mm for 115 V...T 0.4 A; 250 V; 5 x 20 mm (6) Light source Halogen lamp ... HAL 6 V 20 W Adjustment of the light source ... continuous, 1.5 to 6 V DC 1 Color temperature at 6 V ... 2800 K Light flux ... 280 lm Average life ... 1000 h Luminous area ...2.0 x 2.0 mm (7)
Opto-mechanical data 2
Stand with stage focusing ... with coarse drive (4mm/rot) ... and fine drive (0.4mm /rot) ...Overall lift 15 mm Objective change ... manually via 5x nosepiece Objectives... ICS line of objectives with W 0.8" thread Eyepieces... 30 mm plug-in diameter with field number 18... Pl 10x/18 Br. and Pl 10x/18 Br. foc. or with field number 20... E-Pl 10x/20 Br. and E-Pl 10x/20 Br. foc. Specimen stage ... mechanical stage 75x30 R with ceramic surface Dimensions (width x depth) ...160 x 140 mm Travel range (width x depth) ...75 x 30 mm Specimen holder ...with spring clip to the right or for one-handed operation Swing-in condenser 0.9 Z... for M Obj. < 10x swing out condenser front lens 0.9 ... for MObj. 10x swing in condenser front lens 0.9 Binocular tube 45°/20 ICS maximum field number... 20 interpupillary distance... can be set between 55 and 75 mm Viewing angle ...45° Viewing height ...approx. 440 mm Visual port...tube factor 1x
1
For photography using artificial light color reversal film for 3200 K, the conversion filter produces the correct color temperature in the light path.
2
The scale on the coarse drive (0 to 400) permits the orienting measurement of the object thickness: 1 increment corresponds to approx. 5 µm
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CB 3 (467852)
1-11
Standard 25 ICS
Binocular phototube 35°/20 maximum field number ... 20 interpupillary distance ... can be set between 55 and 75 mm viewing angle... 35° viewing height ... approx. 430 mm visual port... tube factor 1x camera/video port ... tube factor 1x camera/video port ... interface 60 mm switched via sliding prism ... 100% obs / 50% obs : 50% doc
1-12
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