Carl Zeiss
Fisher Bioblock Scientific Stereomicroscopes
Stemi 1000-2000-2000C Stereomicroscope Operating Manual Dec 1998
Operating Manual
68 Pages
Preview
Page 1
Stemi 1000/2000/2000-C Stereo Microscopes
Operating Manual
12
Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. The specifications are subject to change; the manual is not covered by an update service.
Unless expressly authorized, forwarding and duplication of this document, and the utilization and communication of its contents are not permitted. Violations will entail an obligation to pay compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
Issued by:
Carl Zeiss Mikroskopie D-07740 Jena Telephone (**49) 03641 / 64-1616 Telefax: (**49) 03641 / 64-3144 Internet: [email protected] http://www.zeiss.de
Number of this manual:
B 40-002 e
Date of issue:
12/98
Stemi 1000, Stemi 2000, Stemi 2000-C
TABLE OF CONTENTS Page Title Copyright Table of contents...
I
List of figures...
III
Note...
IV
General view...
V
Notes on device safety...
VII
1
DESCRIPTION
1.1
Designation, purpose...
1-1
1.2
Description of the units...
1-2
1.3
Technical data...
1-4
1.4
Overview of modules...
1-8
1.5
Function elements...
1-29
2
OPERATION
2.1
Unpacking and setting up...
2-1
2.2
Commissioning...
2-3
2.2.1
Working with accessories...
2-4
3
FLUORESCENCE CONTRAST WITH STEREO MICROSCOPES
3.1
FL S (Fluorescence for Stereo microscope) configuration...
3-2
3.1.1
Name and intended application...
3-2
3.2
Configurations...
3-3
3.2.1
FL S configuration with KL 1500 electronic (not suitable for UV excitation) . . .
3-4
3.2.2
FL S configuration with HBO 50 or HBO 100...
3-6
3.2.3
FL S configuration with LUMATEC HBO 200...
3-8
B 40-002 e 12/98
I
Stemi 1000, Stemi 2000, Stemi 2000-C
4
CARE AND TROUBLESHOOTING
4.1
Care...
4-1
4.2
Troubleshooting and service...
4-2 Page
ANNEX –
List of abbreviations...
A-3
–
Certification in accordance with DIN ISO 9001/EN 29001/EN 46001...
A-5
–
EC conformity declaration...
A-7
II
12/98 B 40-002 e
Stemi 1000, Stemi 2000, Stemi 2000-C
LIST OF FIGURES Page Figure 1-1
Principal assemblies of the Stemi 2000-C...
1-2
Figure 1-2
Optical schematic of the Stemi 2000-C...
1-3
Figure 1-3
Basic microscope equipment...
1-8
Figure 1-4
Eyepieces/eyepiece cups/reticules/attachment systems/specimen measuring plate...
1-10
Figure 1-5
Stands...
1-12
Figure 1-6
Specimen stages...
1-14
Figure 1-7
Halogen lighting systems for vertical illumination...
1-16
Figure 1-8
Cold light sources for vertical illumination...
1-18
Figure 1-9
Halogen lighting systems for transmitted illumination...
1-20
Figure 1-10
Cold light sources for transmitted illumination...
1-22
Figure 1-11
Polarisation systems...
1-24
Figure 1-12
Documentation systems using photo cameras...
1-26
Figure 1-13
Adapters for video cameras...
1-28
Figure 1-14
Function elements of the Stemi 2000-C...
1-30
Figure 2-1
Stemi 2000-C packing unit in city box...
2-1
Figure 2-2
Unpacking...
2-2
Figure 2-3
Setting up the Stemi 2000-C...
2-2
Figure 2-4
Adjusting the Stemi 2000-C...
2-3
Figure 2-5
Inserting a graticule...
2-4
Figure 2-6
Fitting an attachment system to the Stemi 2000...
2-4
Figure 2-7
Mounting the polariser...
2-5
Figure 2-8
Mounting a reflex camera...
2-6
Figure 2-9
Mounting the microscope camera MC 80 for 35 mm photography...
2-7
Figure 2-10
Mounting a video camera...
2-8
B 40-002 e 12/98
III
Stemi 1000, Stemi 2000, Stemi 2000-C
Page Figure 3-1
Stemi 2000 with KL 1500 electronic...
3-2
Figure 3-2
Stemi SV 6 with HBO 100...
3-2
Figure 3-3
Stemi 2000 with HBO 50...
3-2
Figure 3-4
Stemi SV 11 with LUMATEC HBO 200...
3-2
Figure 3-5
FL S configuration with KL 1500 electronic...
3-4
Figure 3-6
...
3-5
Figure 3-7
...
3-5
Figure 3-8
...
3-5
Figure 3-9
...
3-5
Figure 3-10
FL S configuration with HBO 50 or HBO 100...
3-6
Figure 3-11
...
3-7
Figure 3-12
...
3-7
Figure 3-13
...
3-7
Figure 3-14
...
3-7
Figure 3-15
FL S configuration with LUMATEC HBO 200...
3-8
Figure 3-16
...
3-9
Figure 3-17
...
3-9
Figure 3-18
...
3-9
Figure 3-19
...
3-9
Figure 3-20
...
3-10
Figure 3-21
...
3-10
Figure 4-1
Lamp replacement...
4-2
IV
12/98 B 40-002 e
Stemi 1000, Stemi 2000, Stemi 2000-C
NOTE • The figures integrated in the text each have a figure number and a caption, e.g. ”Fig. 2-8” signifies: the figure in Section 2 with the serial number 8. In each figure, details discussed in the text are assigned a reference line marking and an item number. In the running text, ”T2 adapter (2-8/3)”: in Fig. 8 of Section 2, the T2 adapter is marked with the item number 3. • Refer to the annex for explanations of the abbreviations. • This instruction manual refers to the Stemi 1000, Stemi 2000 or the Stemi 2000-C microscope versions (see Page 1-9) and their options. Apply this manual analogously when working with other variants of the unit.
B 40-002 e 12/98
V
Stemi 1000, Stemi 2000, Stemi 2000-C
GENERAL VIEW (Basic unit)
Stemi 1000
Stemi 2000
Stemi 2000-C
VI
12/98 B 40-002 e
Stemi 1000, Stemi 2000, Stemi 2000-C
NEW MODEL We have included a further Stemi model in our line of Greenough-type stereo microscopes.
In response to many requests received from customers, the
Stemi 2000-CS
45 50 55
is now also available as a brand-new item in addition to the Greenough-type stereo microscopes Stemi 1000, Stemi 2000 and Stemi 2000-C. Unlike the Stemi 2000-C, the Stemi 2000-CS (”C” for ”Camera” and ”S” for ”Splitter”) features a
fixed camera port with a 50/50 % beam splitting ratio, i. e. the camera port has no switching lever. Unlike the Stemi 2000-C, the Stemi 2000-CS therefore provides the benefit of
simultaneous stereoscopic viewing while taking photographs or working in the monitor mode. All configurations or accessory components are identical to those of the other Stemi’s.
B 40-002 e 12/98
VII
Stemi 1000, Stemi 2000, Stemi 2000-C
NOTES OF DEVICE SAFETY The Stemi 1000, Stemi 2000 or the Stemi 2000-C stereo microscope including original accessories or options must only be used for the microscopy applications described in this operating manual.
The manufacturer cannot assume any liability for any other applications, possibly also involving individual modules or single parts. This also applies to all service or repair work that is not carried out by authorised service personnel. All guarantee/warranty claims also expire for all those parts that were not directly affected by repair.
Particular attention must be paid to the following warning notes:
The Stemi was designed and tested in conformity with the IEC Publication 1010-1 ”Safety requirements for electrical measuring devices, control equipment and laboratory apparatus” and was delivered in a safe state. This operating manual contains information and warnings which must be followed by the operator.
The Stemi is a light microscope conceived in accordance with the latest scientific and technical knowledge for visual, microphotographic and video analysis of microscopic specimens. The unit must only be used for the intended purpose. It is not intended for continuous unsupervised operation!
The Stemi has no special facilities to protect against samples that have caustic, toxic, radioactive or other effects that are damaging to health.
Users must check whether the available power supply agrees with the value specified on the rating plate of the respective power supply unit.
The power supply unit belonging to the Stemi is a protective class II unit.
Internal parts can carry hazardous voltages when a power supply unit is connected to the mains. The power supply unit must therefore be disconnected from the mains before opening it.
Make sure that only those fuses are used as spares for the respective power supply unit that are actually intended for the prescribed rated current and the specified version. It is forbidden to use provisional fuses or to short-circuit the fuse holders.
Modifications to the unit to keep in line with technical progress are always reserved.
VIII
12/98 B 40-002 e
Stemi 1000, Stemi 2000, Stemi 2000-C
1
Description
1.1
Designation, purpose
Manufacturer ’s designation: stereo microscope Stemi 1000 stereo microscope Stemi 2000 stereo microscope Stemi 2000-C stereo microscope Stemi 2000-CS Brief designation:
Stemi 1000 Stemi 2000 Stemi 2000-C Stemi 2000-CS
Within the product family of stereo microscopes from Carl Zeiss, these units fit in as follows: • Greenough type:
Stemi D Stemi DRC
• Telescopic type:
Stemi SV 6 Stemi SV 11 Stemi SV 11 Apo
Stemi 1000 Stemi 2000 Stemi 2000-C Stemi 2000-CS Stemi 1000 and Stemi 2000 are universally applicable Greenough type stereo microscopes that unite a high imaging quality, compact ergonomic design and internationally standardised connection and coupling points. While the Stemi 1000 and Stemi 2000 are units for visual stereoscopic observation, with its trinocular tube the Stemi 2000-C offers all possibilities of micrographic and video image documentation. Unlike the Stemi 2000-C, the Stemi 2000-CS features a fixed camera port with a 50/50 % beam splitting ratio, i. e. the camera port has no switching lever. Unlike the Stemi 2000-C, the Stemi 2000-CS therefore provides the benefit of simultaneous stereoscopic viewing while taking photographs or working in the monitor mode. Both stereo microscopes’ areas of use cover the following focal areas, among other things: • Materials technology and sciences • Biotechnology and medical technology • Semiconductors industry and research • Hobby and leisure IMPORTANT The units may only be used for the work and applications described in this manual. Refer also to the product liability declaration (Page VIII).
B 40-002 e 12/98
1-1
Stemi 1000, Stemi 2000, Stemi 2000-C
1.2
Description of the units
The Stemi 1000, Stemi 2000 and Stemi 2000-C are highly powerful stereo microscopes that are distinguished by compact and ergonomic design. Figure 1-1 shows the principal assemblies of the Stemi 2000-C. • Stand S with 260 mm column (1-1/4) • Stemi mount with drive for column 32 (1-1/6) • Stemi 2000-C microscope body (1-1/2) • Eyepieces W-PL 10x/23 Spec. foc. (1-1/1) with eye cup • Built-in 6 V, 10 W halogen vertical illumination (1-1/5) with 115/230 V – 6 V 50 VA power supply (1-1/3)
1
2 6 3 5 4
Figure 1-1
1-2
Principal assemblies of the Stemi 2000-C
12/98 B 40-002 e
Stemi 1000, Stemi 2000, Stemi 2000-C
Figure 1-2 schematically shows the optical beam path in the Stemi 2000-C. The unit is switched over between the right tube connector and the camera output with the aid of an adjustable reflector group located under the tube system that features 100/0 % and 0/100 % intensities.
Figure 1-2
Optical schematic of the Stemi 2000-C
B 40-002 e 12/98
1-3
Stemi 1000, Stemi 2000, Stemi 2000-C
1.3
Technical data
(1) Dimensions and weight Mikroscope • Stemi 1000 Dimensions (width x depth x height)... 65 x 336 x 371 mm Weight (with power supply, mains cable and lamp)... 4.33 kg • Stemi 2000 Dimensions (width x depth x height)... 65 x 336 x 371 mm Weight (with power supply, mains cable and lamp)... 4.53 kg • Stemi 2000-C Dimensions (width x depth x height)... 65 x 336 x 371 mm Weight (with power supply, mains cable and lamp)... 4.8 kg • Stand S Support area (width x depth)... 180 x 270 mm Weight... 1.3 kg • Stand S and power supply unit 230 V - 6 V 50 VA Support area (width x depth)... 263 x 270 mm Weight... 1.96 kg City Box Dimensions (closed) (width x depth x height)... 560 x 450 x 240 mm Weight (unequipped)... 4.75 kg
(2) Ambient conditions Storage and transport (in packaging) Permissible ambient temperature... -40 to +70 °C Permissible relative humidity... v 100 % Permissible atmospheric pressure... 500 to 1060 mbar Permissible sinusoidal oscillations... 10 to 150 Hz; 0.5 g Permissible impact (handling impact)... 30 g; 6 ms
1-4
12/98 B 40-002 e
Stemi 1000, Stemi 2000, Stemi 2000-C
Operation Permissible ambient temperature... +15 to +35 °C Permissible relative humidity... v 85 % Permissible atmospheric pressure... 800 to 1060 mbar Permissible impact (handling impact without... 10 g; 6 ms; 100 impacts in operating unit packaging) position (3) Operating data 230 V – 6 V 50 VA power supply (Order No. 458420) 120 V – 6 V 50 VA power supply (Order No. 458421) Mains voltage... 120 V or 230 V Permissible mains voltage fluctuation... +6/-10 % Mains frequency... 50/60 Hz Power consumption... max. 50 VA Light source... halogen 6 V 10 W Variation range... 2.4 ... 5.8 V continuously Class of protection... II Electrical degree of protection... IP 20 Electric safety... in acc. IEC 1010-1 RFI suppression... in acc. EN 55011 Class B Power supply unit, stabilised 6 V 20 W, 115 V – 230 V, variable 1.5 ... 6 V, 50 ... 60 Hz, 40 VA (Order No. 458415) Mains voltage... 100 V, 115 V, 220 V, 240 V, switchable Permissible mains voltage fluctuation... "10 % Mains frequency... 50 ... 60 Hz Power consumption... 40 VA Light source... halogen 6 V 20 W Variation range... 1.5 ... 6.0 V continuously Class of protection... I Electrical degree of protection... IP 40 Electric safety... in acc. IEC 1010-1 RFI suppression... in acc. EN 55011 Class B
B 40-002 e 12/98
1-5
Stemi 1000, Stemi 2000, Stemi 2000-C
Specimen stages, adjustment ranges Sliding stage... "20 mm, rotatable by 360° Spherical stage... "30° horizontal tilt angle, rotatable by 360° Rotary stage... rotatable by 360° Specimen guide D... 28 x 75 mm Expandable stage with specimen guide and holding frame... 76 x 50 mm Stage inlay plates Outer diameter... 84 mm Outer diameter (rotary stage)... 72 mm Stemi mount with drive for column 32 Focusing by means of focusing drive... 46 mm Mount for microscope body... ∅ 76 mm
(4) Optical and mechanical data Stemi 1000 microscope body Effective principle... according to GREENOUGH with optical compensation Stereo angle... 11° Zoom range of the pancratic lens... 0.7x ... 3.5x Free working distance (without dust glass)... 112 mm Viewing angle... 35° Adjustable eye distance... 55 to 75 mm Mount diameter... 76 mm Stemi 2000/2000-C microscope body Effective principle... according to GREENOUGH with mechanical compensation Stereo angle... 11° Zoom range of the pancratic lens... 0.65x ... 5.0x Free working distance (without dust glass)... 92 mm Viewing angle... 35°
1-6
12/98 B 40-002 e
Stemi 1000, Stemi 2000, Stemi 2000-C
Adjustable eye distance... 55 to 75 mm Mount diameter
... 76 mm
Replaceable optical systems Eyepieces... W-PL 10x/23 Br. spect. foc. W 10x/21 foc. W-PL 16x/16 Br. spect. foc. W 25x/10 foc. Attachment systems, fixed... ’ = 0.3x ’ = 0.4x ’ = 0.63x ’ = 2.0x
FOD = 285 mm FOD = 210 mm FOD = 130 mm FOD = 31 mm
Stemi 1000 visual viewing magnification ranges Basic microscope equipment... 7x ... 35x with replaceable optical system... 2,1x ... 175x Stemi 2000/2000-C visual viewing magnification ranges Basic microscope equipment... 6.5x ... 50x with replaceable optical system... 1.95x ... 250x VSS
Okular W 10x/21 foc.
0.3x
0.4x
285
210
W-PL 10x/23 spect. foc.
W-PL 16x/16 spect. foc.
W 25x/10 foc.
Total magnification
Specimen field (mm)
Total magnification
Specimen field (mm)
Total magnification
Specimen field (mm)
Total magnification
Specimen field (mm)
2.1x...10.5x
100.0...20.0
2.1x...10.5x
109.5...21.9
3.4x...16.8x
76.2...15.2
5.3x...26.3x
47.6...9.5
1.95x ... 15.0x
107.7...14
1.95x... 15.0x
118...15.3
3.1x...24.0x
82.1...10.7
4.9x...37.5x
51.3...6.7
2.8x...14.0x
75.0...15.0
2.8x...14.0x
82.1...16.4
4.5x...22.4x
57.1...11.4
7.0x...35.0x
35.7...7.1
2.6x...20.0x
80.8...10.5
2.6x...20.0x
88.5...11.5
4.2x...32.0x
61.5...8.0
6.5x...50.0x
38.5...5.0
4.4x...22.1x
47.7x...9.5
4.4x...22.1x
52.2...10.4
7.1x...35.3x
36.3...7.3
11.0x...55.1x
22.7...4.5
0.63x
130
4.1x...31.5x
51.3...6.7
4.1x...31.5x
56.2...7.3
6.6x...50.4x
39.1...5.1
10.2x...78.8x
24.4...3.2
1x
112 92
7.0x...35.0x
30.0...6.0
7.0x...35.0x
32.9...6.6
11.2x...56.0x
22.9...4.6
17.5x...87.5x
14.2...2.9
6.5x...50x
32.3...4.2
6.5x...50x
35.4...4.6
10.4x...80.0x
24.6...3.2
16.3x...125.0x
15.4...2.0
31
14.0x...70.0x
15.0...3.0
14.0x...70.0x
16.4...3.3
22.4x...112.0x
11.4...2.3
35.0x...175.0x
7.1...1.4
13x...100.0x
16.2...2.1
13x...100.0x
17.7...2.3
20.8x... 60x
12.3...1.6
32.5x...250x
7.7...1.0
2x
Stemi 2000
Stemi 1000
Microphotographic magnification scales for MC 80 (24 x 36 mm miniature format) Basic microscope equipment... from 1.62:1 to 12.5:1 with replaceable optical system... from 0.49:1 to 25:1
B 40-002 e 12/98
1-7
Stemi 1000, Stemi 2000, Stemi 2000-C
1.4
Overview of modules
Basic microscope equipment 1.2
2.2
1.1
2.1 2.3 2.4
1.3 2
1
2.6
1.5
2.7
2.5
1.4
3.2
3.1 3.3 3.4 3 3.6
3.7
3.5
Figure 1-3
1-8
Basic microscope equipment
12/98 B 40-002 e
Stemi 1000, Stemi 2000, Stemi 2000-C
Order No.
Microscope equipment 1
Stereo microscope Stemi 1000 consisting of • Stemi 1000 microscope body • Eyepiece W 10x/21 foc. 2x • Attachment systems adapter (contained in 455050) • Stemi mount with drive for column 32 • Stand S, with column 32, 260 mm long • Dust cover K
1.1 1.2 1.3 1.4 1.5 2
455050 455042 455094 455104 459300
2.7 not illus.
Stereo microscope Stemi 2000 with built-in 6 V, 10 W halogen vertical illumination (120 V)
495102 9904
3
Stereo microscope Stemi 2000-C with built-in 6 V, 10 W halogen vertical illumination (230 V) consisting of • Stemi 2000-C microscope body • Eyepiece W-PL 10x/23 spectacle focusing 2x • Eyepiece cup 2x • Stemi mount with drive for column 32 • Stand S with column 32, 260 mm long • Built-in 6 V, 10 W halogen vertical illumination (230 V) • Dust cover K
495104 9804
Stereo microscope Stemi 2000-C with built-in 6 V, 10 W halogen vertical illumination (120 V) but with: • 120 V – 6 V 50 VA power supply with mains cable and American flat plug
495104 9904
3.1 3.2 3.3 3.4 3.5 3.6 3.7 not illus.
not illus.
Stereo microscope Stemi 2000-CS with stand N and universal vertical illumination (230 V) (115 V)
Stemi 2000-C
495102 9804
2x 2x
Stemi 2000
Stereo microscope Stemi 2000 with built-in 6 V, 10 W halogen vertical illumination (230 V) consisting of • Stemi 2000 microscope body • Eyepiece W-PL 10x/23 spectacle focusing • Eyepiece cup • Stemi mount with drive for column 32 • Stand S with column 32, 260 mm long • Built-in 6 V, 10 W halogen vertical illumination (230 V) • Dust cover K
2.1 2.2 2.3 2.4 2.5 2.6
Stemi 1000
455052 455043 444801 455094 455104 485001 9804
459300
455053 455043 444801 455094 455104 485001 9804
459300
458421
000000 1006 130 000000 1006 132
Part of the microscope equipment Unit capable of functioning in conjunction with the microscope equipment
B 40-002 e 12/98
1-9
Stemi 1000, Stemi 2000, Stemi 2000-C
Accessories (1) Replaceable optical systems
2 1
1.1
2.1
2.1
1.2
1.3
1.4
3.1
4.1 3 4 5 6 7
4 5 6
3.2
4.2 2 3 4 5 6 7 8 2 3 4 5 6 7 8
3.3
4
4.3
3.4
3 8
7
6
8
7
6
3.5 54 3 2 1
0
5
10
15
20
25
mm
3.6 5.1
6.1
6.2
6.3
6.4
6.5
3
Figure 1-4
1-10
Eyepieces/eyepiece cups/reticules/attachment systems/specimen measuring plate
12/98 B 40-002 e