User Manual
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R65 Trolley Instruction Manual
User manual
R65 Trolley with warmed table top
33118 version 1.0
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TABLE OF CONTENTS 1. General information and service . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2. Unpacking and inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3. Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
3.1 Standard accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3.2 Optional equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 4. User Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 4.1 Definition of use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 4.2 General description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 5. Features and operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 5.1 Assembling the R65 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 5.2 Connection to the mains . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 5.3 Start of apparatus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 5.4 Keyboard functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 5.5 Operating the heated area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 5.6 Temperature setting and control . . . . . . . . . . . . . . . . . . . . . . . . . 8 5.7 Combination keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 5.8 Temperature alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 5.9 Warming up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 5.9.1 Safety barrier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 6. Menu function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 6.1 Overview of options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 UNIT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 RS232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Tn-t/Tn-b . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 INT.T . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 Ti.St . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 St.St . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 HEAT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 A-St . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 HOUR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 REST . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 7. User maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 7.2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 8. Trouble shooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 9. Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 10. Spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 10.1 List of spare part numbers . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 10.2 Spare parts - exploded view . . . . . . . . . . . . . . . . . . . . . . . . . . 25 11. Limited warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 12. Liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 13. Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
R65 Trolley Instruction Manual
1. General information and service COPYRIGHT This manual contains information that is subject to copyright. All rights reserved. This manual should not be photocopied, otherwise copied or distributed, completely or in part, without the approval of K-SYSTEMS Kivex Biotec. Users of K-SYSTEMS Kivex Biotec products should not hesitate to contact us if there are any unclear points or ambiguities in this manual.
© K-SYSTEMS Kivex Biotec Ltd Klintehøj Vænge 3-5 3460 Birkerød Denmark Tel.: +45 45 99 56 00 Fax: +45 45 99 56 19 E-mail: [email protected] Internet: www.k-systems.dk Version 1.0 06/2015
CAUTION If the equipment is used in a manner not specified by this manual, the safety of the user may be at risk and the equipment may be damaged. Always use the equipment as outlined in this instruction manual.
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R65 Trolley Instruction Manual
2. Unpacking and inspection • Unpack the equipment. • Check the packing list to insure all accessories are there. • Examine the packing material carefully for separately packed items. • Check the trolley for external surface damage. • Check that the display is not broken or damaged.
3. Accessories 3.1 Standard accessories PSU External power supply Order code: 24009
Mains Cable Standard Schuko - Order code: 52768 UK - Order code: 53886 US - Order code: 52773
3.2 Optional equipment Optional equipment consists of a range of warming blocks. Please contact your local distributor or K-SYSTEMS Kivex Biotec for details about the full range of warming blocks.
Examples of different types of warming blocks Warming block B01 Holds seven tubes of Ø17 mm Falcon 352001 Order code: 26001
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R65 Trolley Instruction Manual
Warming block B06 Holds twelve tubes of Ø12 mm Falcon 352003 NUNC 343923 Order code: 26006
Warming block B09 Holds two tubes of Ø17 mm Falcon 352095 Order code: 26018
Warming block B28 Holds one 4-well dish NUNC 176740/14444 Order code: 26029
Warming block B30 Holds five flushing syringes of 20 ml/Ø22.5 mm Order code: 26013
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R65 Trolley Instruction Manual
4. User Manual 4.1 Definition of use The K-SYSTEMS Kivex Biotecs R65 Trolley is designed for warming the media used during oocyte retrieval in both human and animal assisted reproduction. It can also be used for maintaining a stable temperature of cells and specimens in a test tube/syringe or similar.
4.2 General description The stainles steel surface maintains a preset temperature in the range ambient to max 42° C. The temperature is electronically controlled by a PID controller and can be read on the display in increments of 0.1° C.
RS 232 connector
PSU socket
Heated surface
External powersupply
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Heated drawer
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R65 Trolley Instruction Manual
5. Features and operation 5.1 Assembling the R65 No assembly needed. Just follow directions in 5.2 and the unit is now ready for use.
5.2 Connection to the mains The power supply is automatically regulated to the mains voltag. Connect the R65 to the mains supply cable lead. For safety reasons the unit has an external PSU and operates at a low voltage.
5.3 Start of apparatus Connect the Unit to the PSU and the PSU to the mains wall socket. To power up the unit, press the heat on button on the keyboard. The display indicates the current temperature, either on the table plate or in the drawer.
5.4 Keyboard functions
Alarm Heat ON/OFF LED Display Setpoint Arrow keys UP/DOWN
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R65 Trolley Instruction Manual
5.5 Operating the heated area Action Press heat ON/OFF key to show the actual temperature on either the table plate or in the drawer. Press arrow UP or arrow DOWN to shift between these temperatures.
Key
Temperature on table plate.
Temperature in drawer.
5.6 Temperature setting and control The display indicates the average temperature of the surface.
Action Press setpoint key until set temperature is shown.
Key
Press and hold setpoint key.
While holding the setpoint key in, press either the arrow UP or arrow DOWN key to raise or lower the set temperature. When the desired temperature is reached, let go of setpoint key.
Note
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The setpoint can be adjusted to a reading between ambient and 49,9° C.
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R65 Trolley Instruction Manual
5.7 Combination keys Action Press alarm and UP key.
Key
Display The keyboard is locked.
Press alarm and UP key.
The keyboard is unlocked.
Press Heat ON/OFF and setpoint key.
Switches between temperature and time. The heat in the drawer is turned off.
Press DOWN key and Heat ON/OFF.
Press DOWN key and Heat ON/OFF.
The heat in the drawer is turned back on.
5.8 Temperature alarm The temperature alarm indicates too high or too low temperature. The alarm will be activated if the temperature rises or falls more than 0.5° C from the setpoint. There will be an acoustic signal and the red LED light in the alarm key will be activated.
Action Press alarm key to mute the acoustic signal
Key
Press alarm key once again to bring the signal back on.
Note The red LED light and the acoustic signal will turn off when the temperature stabilises to the setpoint level.
5.9 Warming up Warming up from 20.0 to 37. 0° C takes less than 20 minutes, under normal conditions. When a cold item is placed on the heated area it may need up to 30 minutes to stabilise, depending on the thermal mass of the object and the initial temperature.
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R65 Trolley Instruction Manual
5.9.1 Safety barrier There is a greater heat loss from the edges of the warming plate, and the tolerances can not be kept as precisely in these areas. A high accuracy can only be maintained in the centre area of the warming plate. Please see picture below:
Top view
Our general recommendations: Dark grey area only: +/- 0,3 degrees Celcius Light grey area: +/- 0,8 degrees Celcius White area: No heat
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R65 Trolley Instruction Manual
6. Menu function The user can access a number of advanced functions via the menu.
Action Press and hold UP+DOWN key for 3 seconds to enter the menu.
Key
Display
The first option "UNIT" will appear in the display. Press UP/DOWN key to navigate within the menu. While holding the setpoint key in, press either the arrow UP or arrow DOWN key to change values within the options. Press and hold UP+DOWN key for 3 seconds again to exit the menu.
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R65 Trolley Instruction Manual
The menu consist of the 11 options listed below. For further detail see the description on the following pages.
6.1 Overview of options
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R65 Trolley Instruction Manual
UNIT In the UNIT option the displayed temperature unit can be set to either degrees Celsius or Fahrenheit. Follow these steps to navigate in the UNIT option.
Action Press and hold UP+DOWN key for 3 seconds to enter the menu.
Key
Display
The option "UNIT" will appear in the display. Press and hold setpoint key to change values. While holding the setpoint key in, press either the arrow UP or arrow DOWN key to select the required type of unit. When the required type has been selected let go of the setpoint key. Press and hold UP+DOWN key for 3 seconds to exit the menu.
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R65 Trolley Instruction Manual
RS232 The RS232 option is for serial communication and data logging of the temperature and alarm conditions. Follow these steps to navigate in the RS232 option.
Action Press and hold UP+DOWN key for 3 seconds to enter the the menu.
Key
Display
The option "UNIT" will appear in the display. Press DOWN key until the option "RS232" appears in the display. Press and hold setpoint key to change values. While holding the setpoint key in, press either the arrow UP or arrow DOWN key to select between "ON" and "OFF". "OFF" : The RS232 function is turned off. "ON": Data is send once every two seconds. When the required setting is activated, let go of the setpoint key. Press and hold UP+DOWN key for 3 seconds to exit the the menu.
Data logging the temperature valves during use:
Note
The RS232 cable must be connected before start.
Follow the steps in the table above and choose "ON". Choose one of the settings to get the required data
Note
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S-1
Sends the data for the last one hour.
S-2
Sends the data for the last two hours
send
Sends the data for the last five hours. The use of this function warrants extra equipment software (Ver. 3) and on some base models addition of a Serial PCB. The parameters on or off will not affect normal usage when serial communication is not connected.
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R65 Trolley Instruction Manual
Tn-t/Tn-b The Tn-t/Tn-b option is for calibration of the displayed temperature of the table top (Tn-t) and the drawer (Tn-b). If there is an offset between the value on the display and any measurements made with a high precision external temperature sensor, this can be corrected. The new temperature reading will be kept as the displayed value with temperature controlling conducted on this basis. Follow these steps to navigate in the Tn-t/Tn-b option.
Action Press and hold UP+DOWN key for 3 seconds to enter the menu.
Key
Display
The option "UNIT" will appear in the display. Press DOWN key until the option "Tn-t/Tn-b" appears in the display. Press and hold setpoint key to change values. While holding the setpoint key in, press either the arrow UP or arrow DOWN key to select the value of temperature. When the required temperature has been selected let go of the setpoint key. Press and hold UP+DOWN key for 3 seconds to exit the menu.
CAUTION
Any change in the factory set calibration should always be based on very certain temperature measurements. K-SYSTEMS Kivex Biotec only recommend the use of a high quality PT-100 sensor that is calibrated with the precision instrument used for measuring according to the manufacturers specifications. Ensure an optimal contact between the sensor and the place measured. Use only a sensor type that is correct for the purpose, and which has the correct size. If in doubt, contact your local distributor or K-SYSTEMS Kivex Biotec.
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R65 Trolley Instruction Manual
INT.T The INT.T (Integral Time) option is for changing the base value for the PID controller. This should not be attempted by unauthorised persons. If set at a different level the controlling principle will be affected. From the factory it is set to a closely calculated value specific for the model.
CAUTION Do not change the value here. If by mistake any changes are made or doubts occur if the value are correct please see the REST function in the menu for the restore of the defaults
Ti.St The Ti.St (Time Set) option is for setting the time. Follow these steps to navigate in the Ti.St option.
Action Press and hold UP+DOWN key for 3 seconds to enter the menu. The option "UNIT" will appear in the display. Press DOWN key until the option "Ti.St" appears in the display. Press and hold setpoint key to change values. While holding the setpoint key in, press the arrow UP to change the minutes and arrow DOWN key to change the hour. When the time is set, let go of the setpoint key. Press and hold UP+DOWN key for 3 seconds to exit the menu.
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Key
Display
R65 Trolley Instruction Manual
St.St The St.St (Start Set) option is the timer function for the heat. By using this option, time can be saved as the R65 can be ready for use before procedure start in the morning.
Note
This function will only work correctly if the clock is set.
Follow these steps to navigate in the St.St option.
Action Press and hold UP+DOWN key for 3 seconds to enter the menu.
Key
Display
The option "UNIT" will appear in the display. Press DOWN key until the option "St.St" appears in the display. Press and hold setpoint key to change values. While holding the setpoint key in, press the arrow UP to change the minutes and arrow DOWN key to change the hour. When the time is set, let go of the setpoint key. Press and hold UP+DOWN key for 3 seconds to exit the menu.
Note
If the function Heat is set, a dot will light up in the right side of display.
St.St is connected with HEAT. It must be chosen when the timer starts. To turn the heater on, please see the option HEAT.
WARNING
Always make sure when the timer function is being used, that the heated area is clear of any objects that might be damaged by the heat or adversely affected by it in any way. Caution should always be exercised when a heated area is turned on without any supervision.
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R65 Trolley Instruction Manual
HEAT The HEAT option is used for the heater to turn on at a certain time.
Note
This function only works in conjunction with St.St. If St.St is set to 8.00 and heat function is on, the heater will automatically turn on at 8.00 using 24 hour clock.
Follow these steps to navigate in the HEAT option.
Action Press and hold UP+DOWN key for 3 seconds to enter the menu.
Key
Display
The option "UNIT" will appear in the display. Press DOWN key until the option "HEAT" appears in the display. Press and hold setpoint key to change values. While holding the setpoint key in, press either the arrow UP or arrow DOWN key to select between "ON" and "OFF". When the required setting is activated, let go of the setpoint key. Press and hold UP+DOWN key for 3 seconds to exit the menu.
Note
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A dot will turn on in the right side of display, indicating that the Heat function is active.
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R65 Trolley Instruction Manual
A-St The A-St (Automatic Start) option is used to repeat the timer (St.St) function every day of the week.
Note
This function works in conjunction with St.St and HEAT. If St.St is set to 8.00 and heat function is on, the heater will automatically turn on at 8.00 every day.
Follow these steps to navigate in the A-St option.
Action Press and hold UP+DOWN key for 3 seconds to enter the menu.
Key
Display
The option "UNIT" will appear in the display. Press DOWN key until the option "A-st" appears in the display. Press and hold setpoint key to change values. While holding the setpoint key in, press either the arrow UP or arrow DOWN key to select between "ON" and "OFF". When the required setting is activated, let go of the setpoint key. Press and hold UP+DOWN key for 3 seconds to exit the menu.
Note
If this function is set to ‘on’ the heat function will be repeated every day, but if A-St function is set to ‘off’ the heat function will only be activated one time.
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R65 Trolley Instruction Manual
HOUR The HOUR option gives the opportunity to show the time on the display when the heat is off. Follow these steps to navigate in the HOUR option.
Action Press and hold UP+DOWN key for 3 seconds to enter the menu.
Key
Display
The option "UNIT" will appear in the display. Press DOWN key until the option "HOUR" appears in the display. Press and hold setpoint key to change values. While holding the setpoint key in, press either the arrow UP or arrow DOWN key to select between "ON" and "OFF". When the required setting is activated, let go of the setpoint key. Press and hold UP+DOWN key for 3 seconds to exit the menu.
If you want to see the time on the display when the heat is turned on. Follow these steps to switch between time and temperature
Action Press and hold setpoint key .
Key
While holding the setpoint key in, press the HEAT key one time, then let go of the setpoint key. The display now shows the time.
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Display