Heratherm IGS series General Protocol Operating Instructions Aug 2012

C Contents Chapter 1 Safety Notes ... 1-1 Basic Operating Precautions ... 1-1 Operational Safety Rules ... 1-2 Warranty ... 1-2 Explanation of Safety Information and Symbols ... 1-3 Safety Notes and Symbols Used Throughout These Operating Instructions ... 1-3 Additional Symbols for Safety Information... 1-4 Symbols on the Incubator... 1-5 Intended Purpose of the Incubator ... 1-5 Correct Use ... 1-5 Incorrect Use ... 1-5 Standards and Directives ... 1-5 Chapter 2 Delivery of the Incubator... 2-1 Packaging ... 2-1 Acceptance Inspection ... 2-1 Scope of Supply ... 2-2 Chapter 3 Installation... 3-1 Ambient Conditions... 3-1 Location Requirements... 3-1 Intermediate Storage... 3-2 Room Ventilation... 3-2 Space Requirements... 3-3 Installation Built-in Units... 3-3 Table-top units ... 3-4 Floor Stand Units ... 3-5 Transport ... 3-7 Stacking kit ... 3-9 Installing the Stacking Feet... 3-9 Installing the Stacking Adapter ... 3-10 Installing the Anti-tilt Anchor ... 3-10 Floor stand unit spacers ... 3-13 Chapter 4 Product Description ... 4-1 Heratherm IGS Series Incubator Overview... 4-1 Safety Devices... 4-8 Work Space Atmosphere ... 4-8 Sensing and Control System ... 4-8 Data Communications & Alarm Interface ... 4-9 RS-232 Interface... 4-9

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Contents

AC Power Socket... 4-10 Fuses... 4-10 Work Space Components ... 4-10 Inner Chamber ... 4-10 Access Port ... 4-10 Tube Access Ports... 4-12 Chapter 5 Start-up ... 5-1 Installing the Shelf System for Table-top Units ... 5-1 Initial Installation ... 5-1 Installing the Perforated Shelves ... 5-2 Preparing the Work Space ... 5-2 Installation of the Support Rails (only for table-top Units) ... 5-3 Installing the Shelf Support Brackets ... 5-3 Installing the Perforated Shelves ... 5-4 Installing/Removing air baffles... 5-5 Levelling the Table-top Incubator Unit ... 5-6 Connecting Power ... 5-6 Connecting to the Power Supply Source... 5-6 Connecting the RS-232 Interface ... 5-7 Chapter 6 Operation... 6-1 Preparing the Incubator... 6-1 Starting Operation... 6-1 Chapter 7 Handling and Control ... 7-1 Powering Up ... 7-5 Switching the Incubator Off / Powering Down ... 7-5 Temperature Set Value ... 7-6 Timer ... 7-7 Stopping a Timer... 7-8 Power Outlet ... 7-10 Settings... 7-11 Error Log... 7-11 Calibration ... 7-12 Temperature Display Unit... 7-13 Chapter 8 Shut-down ... 8-1 Shutting the Incubator Down... 8-1 Chapter 9 Cleaning and Disinfection... 9-1 Cleaning ... 9-1 Wipe / Spray Disinfection ... 9-1 Preparing the Manual Wipe/Spray Disinfection ... 9-2 Predisinfection... 9-3 Cleaning ... 9-3 Final Disinfection ... 9-3 Chapter 10 Maintenance ... 10-1 Inspections and Checks ... 10-1

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Contents

Service Intervals...10-2 Preparing Temperature Calibration...10-2 Comparison Measurement Procedure...10-3 Temperature Calibration Procedure ...10-3 Replacing the Door Seal ...10-4 Replacing the Power Cord...10-4 Returns for Repair...10-5 Chapter 11 Disposal...11-1 Overview of Materials Used ...11-1 Chapter 12 Error Codes...12-1 Chapter 13 Technical Data ...13-1 Chapter 14 Spare Parts and Accessories ...14-1 Chapter 15 Device Log ...15-1 Chapter 16 Contact ...16-1

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L List of Figures Figure 3-1 Table-top Incubators, dimensions and required clearances... 3-4 Figure 3-2 Floor Stand Incubators, dimensions and required clearances... 3-5 Figure 3-3 Floor Stand Incubators, dimensions and required clearances... 3-6 Figure 3-4 Lift Points... 3-7 Figure 4-1 Heratherm IGS 60/IGS 100/IGS 180 Front View... 4-2 Figure 4-2 Heratherm IGS 60/IGS 100/IGS 180 Rear View ... 4-3 Figure 4-3 Heratherm IGS 400 Front View... 4-4 Figure 4-4 Heratherm IGS 400 Rear View... 4-5 Figure 4-5 Heratherm IGS 750 Front View... 4-6 Figure 4-6 Heratherm IGS 750 Rear View... 4-7 Figure 4-7 Sensor System (for table-top units) ... 4-8 Figure 4-8 Sensor System (for floor stand units) ... 4-8 Figure 4-9 Signal Interfaces and Power Socket ... 4-9 Figure 4-10 Shelf System ... 4-11 Figure 4-11 Tube Access Ports... 4-12 Figure 5-1 Sliding the Retaining Spring into the Support Rail ... 5-1 Figure 5-2 Installing the Shelving ... 5-2 Figure 5-3 Support Rail Installation... 5-3 Figure 5-4 Shelf Support Installation ... 5-4 Figure 5-5 Installing the Perforated Shelves ... 5-4 Figure 5-6 Removing the bottom plate ... 5-5 Figure 5-7 Removing the left and right support profiles... 5-5 Figure 5-8 Removing the rear air baffle IGS 400 / 750 ... 5-5 Figure 5-9 AC Power Supply Socket ... 5-7 Figure 7-1 Control Panel for Heratherm IGS Series Incubators ... 7-1 Figure 10-1 Door Seal Replacement ... 10-4

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List of Figures

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Safety Notes Basic Operating Precautions These operating instructions describe Heratherm incubators. Heratherm incubators have been manufactured to the latest state of the art and have been tested thoroughly for flawless functioning prior to shipping. However, the incubator may present potential hazards, particularly if it is operated by inadequately trained personnel or if it is not used in accordance with the intended purpose. Therefore, the following must be observed for the sake of accident prevention: • Heratherm incubators must be operated by adequately trained and authorized professional personnel. • Heratherm incubators must not be operated unless these operating instructions have been fully read and understood. • The present operating instructions, applicable safety data sheets, plant hygiene guidelines and the corresponding technical rules issued by the operator shall be used to create written procedures targeted at personnel working with the subject matter device, detailing: • the decontamination measures to be employed for the incubator and the accessories used with it, • the safety precautions to be taken when processing specific agents, • the measures to be taken in case of accidents. • Repair work on the incubator must be carried out only by trained and authorized expert personnel. • The contents of these operating instructions are subject to change at any time without further notice. • Concerning translations into foreign languages, the German version of these operating instructions is binding. • Keep these operating instructions close to the incubator so that safety instructions and important information are always accessible. • Should you encounter problems that are not detailed adequately in these operating instructions, please contact Thermo Electron LED GmbH immediately for your own safety.

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Safety Notes Operational Safety Rules

Operational Safety Rules The following rules must be heeded when working with Heratherm incubators: • Observe the sample weight limits specified for your Heratherm incubator as a whole and its shelving in particular; see chapter 13, “Technical Data.” • Do not load the bottom of the interior workspace to avoid the risk of overheating any samples placed there and to prevent the temperature sensor from being damaged. • Arrange the samples evenly throughout the work space, making sure not to place them too closely to the interior walls to ensure a uniform temperature distribution. • Do not load your Heratherm incubator with substances that exceed the capabilities of the available lab apparatus and Personal Protection Equipment to provide sufficient degrees of protection to users and third parties. • Check the door seal every six months for proper sealing performance and possible damage. • Do not process any samples containing hazardous chemical substances that may be released into the ambient air through defective seals or may cause corrosion or other defects on parts of the Heratherm incubator.

Warranty Thermo Fisher Scientific warrants the operational safety and functions of the Heratherm incubators only under the condition that: • the incubator is operated and serviced exclusively in accordance with its intended purpose and as described in these operating instructions, • the incubator is not modified, • only original spare parts and accessories that have been approved by Thermo Scientific are used (third-party spares without Thermo Scientific approval void the limited warranty), • inspections and maintenance are performed at the specified intervals, • an operation verification test is performed after each repair activity. The warranty is valid from the date of delivery of the incubator to the customer.

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Safety Notes Explanation of Safety Information and Symbols

Explanation of Safety Information and Symbols Safety Notes and Symbols Used Throughout These Operating Instructions

Indicates a hazardous situation which, if not avoided, will result in death or serious injuries.

Indicates a hazardous situation which, if not avoided, could result in death or serious injuries.

Indicates a situation which, if not avoided, could result in damage to equipment or property.

Is used for useful hints and information regarding the application.

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Safety Notes Explanation of Safety Information and Symbols

Additional Symbols for Safety Information

Wear safety gloves!

Wear safety goggles!

Harmful liquids!

Electric shock!

Hot surfaces!

Fire hazard!

Explosion hazard!

Suffocation hazard!

Biological hazard!

Contamination hazard!

Danger of tipping!

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Safety Notes Intended Purpose of the Incubator

Symbols on the Incubator Observe operating instructions

Mark of conformity USA/Canada

120 Volts AC power socket

Intended Purpose of the Incubator Correct Use Heratherm incubators are laboratory devices for preparing and cultivating cell and tissue cultures. The devices employ precision temperature control for simulating the specific physiological ambient conditions for these cultures.

Incorrect Use To avoid the risk of explosion do not load the incubator with tissue, material, or liquids that: • are easily flammable or explosive, • release vapor or dust that forms combustible or explosive mixtures when exposed to air, • release poisons, • do not pour any liquids on the bottom of the interior surface or into a collecting basin inside the unit. • release dust • exhibit exothermic reactions • are pyrotechnical substances

Standards and Directives The incubator complies with the following standards and guidelines: • IEC EN 61010 - 1, IEC EN 61010 - 2 - 010 • Low Voltage Directive 2006/95/EC

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Safety Notes Standards and Directives

• EMC Directive 2004/108/EC Additionally, the incubator is in compliance with many other international standards, regulations and directives not listed here. Should you have any questions regarding compliance with national standards, regulations and directives applicable for your country, please contact your Thermo Fisher Scientific sales organization.

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Delivery of the Incubator Packaging Heratherm incubators are delivered in a rugged packaging box. All packaging materials can be separated and are reusable: Packaging materials Packaging carton:

Recycled paper

Foam elements:

Styrofoam (CFC-free)

Pallet:

Chemically untreated wood

Packaging film:

Polyethylene

Packaging ribbons:

Polypropylene

Acceptance Inspection After the incubator has been delivered, check the delivery immediately for: • completeness, • possible damage. If components are missing or damage is found on the incubator or the packaging, in particular damage caused by humidity and/or water, please notify the carrier as well as Thermo Scientific Technical Support immediately.

Risk of injury Should sharp edges have formed in damaged areas or elsewhere on the device, take all necessary precautions to protect personnel handling the incubator. For example, have them wear protective gloves and other personal protection equipment.

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Delivery of the Incubator Scope of Supply

Scope of Supply Incubators

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Quantity of components supplied (pieces)

IGS Series

Perforated shelves

2

Support rail for shelf table-top units

4

Power cord

1

Operating manual

1

Shelf support

4

Clip springs for table-top units

4

Anti-tilt anchor

1

Short reference guide

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Installation Ambient Conditions Location Requirements Built-in units of incubators can, heating and drying ovens must be operated with an air exhaust system and exhaust hose (only original Thermo accessory should be used). For safety reasons, the installation space should be made of non-combustible materials, according to DIN 4102. During installation of built-in units, ensure that the escaping air will be safely discharged out of the installation space. The incubator must only be operated in a location that meets all of the ambient condition requirements listed below: • Installation location indoors in dry areas free from drafts. • The dust burden may not exceed the contamination category 2 based on EN 61010-1. Using the incubator in an atmosphere with electrically conductive dust is prohibited. • The minimal distance to adjacent surfaces must be observed on all sides (see section “Space Requirements” on page 3-3) • The operating room must be equipped with appropriate ventilation. • Solid, level, fire-proof surface; no flammable materials opposite to the rear panel of the incubator. • Vibration-proof substructure (floor stand, lab table) capable of bearing the dead weight of the incubator and its accessories (in particular when stacking the table-top units). • The electrical circuitry of the incubator has been designed for an operating height of up to 2000 m above sea level. • Relative humidity up to 80% (maximum; preferably 60-70%), non condensing. • Should condensation exist, wait until the moisture has evaporated completely before connecting the incubator to a power source and powering up. • The ambient temperature must be within a range of +18 °C to +32 °C (64.4 °F to 89.6 °F).

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Installation Intermediate Storage

• Avoid direct exposure to sunlight. • Devices that produce excessive amounts of heat must not be placed near the incubator. • The table-top incubator should not be operated directly on the floor of the lab, but must be placed on a substructure (optional: must be ordered separately). This prevents the penetration of dust or dirt into the device. • Power line voltage variations must not exceed ±10 % of the nominal voltage. • Transient surges must lie within the range of levels that normally occur in the power supply system. The impulse withstand voltage based on surge category II of IEC 60364-4-443 shall be applied at the nominal voltage level. • Consider installing one dedicated upstream circuit breaker per incubator to avoid multiple device failures in case of an electrical fault.

Contamination hazard Do not place the incubator directly on the lab floor, but mount it on the floor stand or on a lab work surface (option; to be ordered separately). Contaminants, such as bacteria, viruses, fungi, prions, and other biological substances may use the open door to migrate easily from the floor into the incubator’s work space.

Intermediate Storage When the incubator is placed in intermediate storage, which is permissible for a maximum of four weeks, make sure that the ambient temperature is between 20 °C to 60 °C (68 °F to 140 °F) and the maximum relative humidity does not exceed 90%, non-condensing.

Room Ventilation Heat dissipating from the incubator during continuous operation may cause a change in the room climate. Therefore, the incubator must only be installed in rooms with sufficient ventilation. Do not install the incubator in room recesses without ventilation. When several devices are to be placed in the same room, additional ventilation may have to be provided as necessary. To avoid any impact of the heat dissipated by the incubator on the ambient climate the room must be vented by means of a laboratory-grade ventilation system that complies with applicable local and national health and safety regulations and has sufficient capacity. If excessive temperatures tend to occur in the operating room, be sure to provide a thermal protection means that cuts out the power supply to mitigate the impact of overtemperature scenarios.

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Installation Space Requirements

Space Requirements For built-in units following clearances should be kept:

A, B, C and D see Operating Instructions of the unit. E (mm/inch)

F (mm/inch)

G (mm/inch)

H (mm/inch)

100 / 4

50 / 2

80 / 3,2

30 / 1,2

Installation Built-in Units IGS

Clearances, without exhaust hose.

IMH/IMH-S

Clearances, close air port with plug, supplied with the unit.

OGS/OMS

Clearances, with additional space to operate to air slide, with air exhaust system and exhaust hose Ø 40 mm (1.58 in)/1.5 m (59 in) (original Thermo accessory), shortened to required length.

OGH/OGH-S OMH/OMH-S

Clearances, with air exhaust system and exhaust hose Ø 40 mm (1.58 in)/1.5 m (59 in) (original Thermo accessory), shortened to required length, operating temperature up to max. 250 °C (482 °F).

After connecting the built-in unit to the electrical power supply, avoid damaging the power cord, then sliding into the installation position. When installing the incubator, make sure that the installation and supply connections remain freely accessible. The specified side clearances represent minimum distances.

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Installation Space Requirements

Table-top units

Figure 3-1 Table-top Incubators, dimensions and required clearances Table 3-1 Incubator Dimensions Model

A (mm/inch*)

B (mm/inch)

C (mm/inch)

D (mm/inch)

IGS 60

530/20.1

565/22.2

720/28.3

540/21.3

IGS 100

640/25.2

565/22.2

820/32.3

650/25.6

IGS 180

640/25.2

738/29.1

920/36.2

650/25.6

* Dimensions in inches are rounded equivalents specified for information only. Depth of handle /display (66 mm/2.6 in) not

included in overall depth specified; height of adjustable feet (36 mm/1.4 in) not included in overall height specified.

Table 3-2 Required Clearances

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E (mm/inch)

F (mm/inch)

G (mm/inch)

H (mm/inch)

80/3.1

50/2.0

200/7.9

300/11.8

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Installation Space Requirements

Floor Stand Units 400 liter units

Figure 3-2 Floor Stand Incubators, dimensions and required clearances Table 3-3 Incubator Dimensions Model

A (mm/inch)

B (mm/inch)

C (mm/inch)

D (mm/inch)

IGS 400

755/29.7

770/30.3

1655/65.2

810/31.9

* Depth of handle /display (66 mm/2.6 in) not included in overall depth specified. Width of hinge (23 mm) not included in overall width.

Table 3-4 Required Clearances E (mm/inch)

F (mm/inch)

H (mm/inch)

I (mm/inch)

120 / 4.7

50 / 2

200 / 7.9

200 / 7.9

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